Porphyrin biosynthesis in Rhodopseudomonas palustris-VIII. Purification and properties of deaminase
1. 1. Uroporphyrinogen I synthetase from Rhodopseudomonas palustris has been isolated and purified. 2. 2. Assay conditions were determined. 3. 3. Sephadex G-100 column chromatography was found to increase 4-fold the degree of purification yielding a deaminase that was purified 72-fold. 4. 4. Some pr...
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1987
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| LEADER | 08966caa a22006977a 4500 | ||
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| 001 | PAPER-839 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203011.0 | ||
| 008 | 190411s1987 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-45949131077 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 100 | 1 | |a Kotler, M.L. | |
| 245 | 1 | 0 | |a Porphyrin biosynthesis in Rhodopseudomonas palustris-VIII. Purification and properties of deaminase |
| 260 | |c 1987 | ||
| 270 | 1 | 0 | |m del C. Batlle, A.M. |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Batlle, Grinstein, Porphyrin biosynthesis I Studies on erythrocyte preparations (1964) Biochimica et Biophysica Acta (BBA) - General Subjects, 82, pp. 1-12 | ||
| 504 | |a Batlle, Rossetti, Enzyme polymerization of porphobilinogen into uroporphyrinogens (1977) Int. J. Biochem., 8, pp. 251-267 | ||
| 504 | |a Battersby, Fookes, Gustafson-Potter, Matcham, McDonald, Proof by synthesis that unrearranged Hydroxymethylbilane is the product from Deaminase and the substrate for cosynthetase in the biosynthesis of Urogen III (1979) Journal of the Chemical Society, Chemical Communications, pp. 1155-1158 | ||
| 504 | |a Battersby, Fookes, Matcham, McDonald, Order of assembly of the four pyyrole rings during biosynthesis of the natural porphyrins (1979) Journal of the Chemical Society, Chemical Communications, pp. 539-541 | ||
| 504 | |a Battersby, Fookes, Matcham, McDonald, Gustafson-Potter, Biosynthesis of the natural porphyrins experiments on the ring-closure steps and with the hydroxy-analogue of porphobilinogen (1979) Journal of the Chemical Society, Chemical Communications, pp. 316-319 | ||
| 504 | |a Battersby, Fookes, McDonald, Meegan, Biosynthesis of Type-III porphyrins proof of intact enzymic conversion of the head-to-tail bilane into Urogen III by intramolecular rearrangement (1978) Journal of the Chemical Society, Chemical Communications, pp. 185-186 | ||
| 504 | |a Bradford, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding (1976) Analyt. Biochem., 72, pp. 248-254 | ||
| 504 | |a Bogorad, Enzymic mechanisms in porphyrin synthesis (1963) Possible enzymatic blocks in porphyrias, 104, pp. 676-680. , Ann. N.Y. Acad. Sci | ||
| 504 | |a Burton, Fagerness, Hosozawa, Jordan, Scott, 13CNMR Evidence for a new intermediate, Pre-uroporphyrinogen, in the enzymatic transformation of porphobilinogen into Uroporphyrinogens I and III (1979) Journal of the Chemical Society, Chemical Communications, pp. 202-204 | ||
| 504 | |a Cohen-Bazire, Sistrom, Stanier, Kinetic studies of pigment synthesis by non-sulfur purple bacteria (1957) J. Cell. comp. Physiol., 49, pp. 25-68 | ||
| 504 | |a Frydman, Feinstein, Studies on porphobilinogen deaminase and uroporphyrinogen III cosynthetase from human erythrocytes (1974) Biochim. biophys. Acta, 350, pp. 358-373 | ||
| 504 | |a Higuchi, Bogorad, The purification and properties of Uroporphyrinogen I synthase and uroporphyrinogen III cosynthetase (1975) Interactions between the enzymes, 244, pp. 401-408. , Ann. N.Y. Acad. Sci | ||
| 504 | |a Jordan, Shemin, Purification of Uroporphyrinogen I synthetase from Rp spheroides (1973) J Biol Chem, 248, pp. 1019-1024 | ||
| 504 | |a Juknat, Kotler, Batlle, Enzymic ciclotetramerization of porphobilinogen into Uroporphyrinogens (1987) Porphyrin biosynthesis in Rp palustris—IV, , In preparation | ||
| 504 | |a Llambias, Batlle, Uroporphyrinogen III cosynthetase (1970) Evidence for the existence of a polypyrrolic substrate in soybean callus tissue, 6, pp. 285-288. , FEBS Lett | ||
| 504 | |a Llambias, Batlle, Studies on the porphobilinogen deaminase-uroporphyrinogen cosynthetase system of cultured soybean cells (1971) Biochem J, 121, pp. 327-340 | ||
| 504 | |a Llambias, Batlle, Porphyrin biosynthesis—VII Avian erythrocyte porphobilinogen deaminase-uroporphyrinogen III cosynthetase its purification properties and the separation of its components (1971) Biochim Biophys Acta, 227, pp. 180-191 | ||
| 504 | |a Lockwood, Rimington, Purification of an enzyme converting porphobilinogen to uroporphyrinogen (1957) Biochem. J., 67, pp. 8-11 | ||
| 504 | |a Lowry, Rosebrough, Farr, Randall, Protein measurement with the folin phenol reagent (1951) J. biol. Chem., 193, pp. 265-275 | ||
| 504 | |a Moore, Labbe, Assays for ALA and PBG determination (1964) Clin. Chem., 10, pp. 1105-1109 | ||
| 504 | |a Polo, Stella, Wider, Batlle, Lenezner, Altuna, Magnin, Porfirinas en la piel de profiria cutánea tardía por cromatografia liquida a alta presión (1983) Prensa Med Mex, 70, pp. 433-440 | ||
| 504 | |a Rimington, Spectral absorption coefficients of some porphyrins in the Soret band region (1960) Biochem J, 75, pp. 620-623 | ||
| 504 | |a Rossetti, Enzymic ciclotetramerization of porphobilinogen (1978) Its mechanism, , Ph. D. thesis, University of Buenos Aires | ||
| 504 | |a Rossetti, Batlle, Polypyrrol intermediates in porphyrin biosynthesis (1977) Studies with Euglena gracilis, 8, pp. 277-283. , Int. J. Biochem | ||
| 504 | |a Rossetti, Juknat, Kotler, Fumagalli, Batlle, Occurrence of multiple molecular forms of porphobilinogenase in diverse organisms: the minimum quaternary structure of porphobilinogenase is a protomer of one deaminase and one isomerase domain (1980) Int. J. Biochem., 12, pp. 761-767 | ||
| 504 | |a Sancovich, Batlle, Grinstein, Porphyrin biosynthesis—VI. Separation and purification of Porphobilinogen deaminase and uroporphyrinogen isomerase from cow liver (1969) Porphobilinogenase an allosteric enzyme, 191, pp. 130-143. , Biochim. biophys. Acta | ||
| 504 | |a Sancovich, Ferramola, Batlle, Grinstein, Preparation of porphobilinogen (1970) Methods in Enzymology, 17 A, pp. 220-222. , H. Tabor, C.W. Tabor, Academic Press, New York | ||
| 504 | |a Sancovich, Ferramola, Batlle, Kivilevich, Grinstein, Studies on cow liver porphobilinogen deaminase (1976) Acta physiol. latinoam., 26, pp. 376-386 | ||
| 504 | |a Williams, Morgan, McDonald, Battersby, Purification of porphobilinogen deaminase from Euglena gracilis and studies of its kinetics (1981) Biochem. J., 193, pp. 301-310 | ||
| 520 | 3 | |a 1. 1. Uroporphyrinogen I synthetase from Rhodopseudomonas palustris has been isolated and purified. 2. 2. Assay conditions were determined. 3. 3. Sephadex G-100 column chromatography was found to increase 4-fold the degree of purification yielding a deaminase that was purified 72-fold. 4. 4. Some properties of the isolated enzyme were studied. The optimal pH was about 7.6-7.8. Porphyrin formation was linear with time. The presence of several thiol reagents was found to be no essential for deaminase activity. 5. 5. Deaminase exhibited classical Michaelis-Menten kinetics Km and Vmax were estimated. 6. 6. Molecular weight determinations, by means of gel filtration on a calibrated Sephadex G-100 column, gave values of 74,000 ± 7400. © 1987. |l eng | |
| 536 | |a Detalles de la financiación: National Council for Scientific Research | ||
| 536 | |a Detalles de la financiación: Acknowledgements--This work was supported by grants from CON~CET (Argentine National Research Council), SUBCYT, Secretaria de Salud Pfiblica del Ministerio de SMud Pflbtica y Banco de la Nacidn Argentina. AIcira M. deI C. Baffle holds the post of Scientific Researcher in CONICET; Mdnica L. Kotlcr, Si[via A. Fumagalli and Adda A. Juknat de Gerainik are a Research Fei1ows from CONICET. This paper forms part of the Doctora[ Thesis of M6nica L Kofler ~o be presented to the University of Buenos Aires for her PhD. We are very grateful to Mrs Dora Beatriz RicciI}o de Aprea and Miss Hilda Gasparoli for their skilful technical assistance. We Mso wish to express our gratitude to Dr M. V. Rossetti for her kind support in some experiments. | ||
| 593 | |a Centro de Investigaciones sobre Porfirinas y Porfirias (CIPYP), (CONICET Facultad de Ciencias Exactas y Naturales, University of Buenos Aires), Ciudad Universitaria, Pabellon II 2 Piso, Buenos Aires, Argentine | ||
| 700 | 1 | |a Fumagalli, S.A. | |
| 700 | 1 | |a Juknat, A.A. | |
| 700 | 1 | |a del C. Batlle, A.M. | |
| 773 | 0 | |d 1987 |g v. 87 |h pp. 601-606 |k n. 3 |x 03050491 |w (AR-BaUEN)CENRE-2752 |t Comparative Biochemistry and Physiology -- Part B: Biochemistry and | |
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