Combined expression patterns of QTL-linked candidate genes best predict thermotolerance in Drosophila melanogaster
Knockdown resistance to high temperature (KRHT) is a thermal adaptation trait in Drosophila melanogaster. Here we used quantitative real-time PCR (qRT-PCR) to test for possible associations between KRHT and the expression of candidate genes within quantitative trait loci (QTL) in eight recombinant i...
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2009
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| LEADER | 12557caa a22012137a 4500 | ||
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| 001 | PAPER-8366 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203810.0 | ||
| 008 | 190411s2009 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-70349172507 | |
| 024 | 7 | |2 cas |a Drosophila Proteins | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a JIPHA | ||
| 100 | 1 | |a Norry, F.M. | |
| 245 | 1 | 0 | |a Combined expression patterns of QTL-linked candidate genes best predict thermotolerance in Drosophila melanogaster |
| 260 | |c 2009 | ||
| 270 | 1 | 0 | |m Norry, F.M.; Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, (C-1428-EHA) Buenos Aires, Argentina; email: fnorry@ege.fcen.uba.ar |
| 506 | |2 openaire |e Política editorial | ||
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| 504 | |a Wright, T.R.F., The genetics of biogenic amine metabolism, sclerotization, and melanization in Drosophila melanogaster (1987) Advances in Genetics, 24, pp. 127-222 | ||
| 504 | |a Zeng, Z.B., QTL mapping and the genetic basis of adaptation: recent developments (2005) Genetica, 123, pp. 25-37 | ||
| 520 | 3 | |a Knockdown resistance to high temperature (KRHT) is a thermal adaptation trait in Drosophila melanogaster. Here we used quantitative real-time PCR (qRT-PCR) to test for possible associations between KRHT and the expression of candidate genes within quantitative trait loci (QTL) in eight recombinant inbred lines (RIL). hsp60 and hsc70-3 map within an X-linked QTL, while CG10383, catsup, ddc, trap1, and cyp6a13 are linked in a KRHT-QTL on chromosome 2. hsc70-3 expression increased by heat-hardening. Principal Components analysis revealed that catsup, ddc and trap1 were either co-expressed or combined in their expression levels. This composite expression variable (e-PC1) was positively associated to KRHT in non-hardened RIL. In heat-hardened flies, hsp60 was negatively related to hsc70-3 on e-PC2, with effects on KRHT. These results are consistent with the notion that QTL can be shaped by expression variation in combined candidate loci. We found composite variables of gene expression (e-PCs) that best correlated to KRHT. Network effects with other untested linked loci are apparent because, in spite of their associations with KRHT phenotypes, e-PCs were sometimes uncorrelated with their QTL genotype. © 2009 Elsevier Ltd. All rights reserved. |l eng | |
| 536 | |a Detalles de la financiación: Umweltbundesamt | ||
| 536 | |a Detalles de la financiación: Natural Sciences and Engineering Research Council of Canada | ||
| 536 | |a Detalles de la financiación: Agencia Nacional de Promoción Científica y Tecnológica | ||
| 536 | |a Detalles de la financiación: We thank Ary Hoffmann for letting us use his SH lines when starting our baselines, Jesper Dahlgaard for earlier collaboration on the set up of QTL lines, Pablo Sambucetii and Alejandra Scannapieco for collaboration on the set up of RIL, Jane Frydenberg for advice with the application of the qRT-PCR procedures, and two anonymous reviewers for helpful comments on the manuscript. This research was supported by frame and center grants from the Danish Natural Sciences Research Council to VL. Additional support from ANPCyT, UBA and CONICET-Argentina to FMN and from the Chinese–Danish Government Scholarship to YJL is also acknowledged. | ||
| 593 | |a Biological Sciences, Ecology and Genetics, University of Aarhus, Ny Munkegade, Bldg. 1540, DK-8000 Aarhus C, Denmark | ||
| 593 | |a College of Plant Protection, Shandong Agricultural University, Daizong Street 61, Tai'an, Shandong 271018, China | ||
| 593 | |a Danish Agricultural Advisory Service, National Centre for Fur Animals, Udkaersvej 15, DK-8200 Aarhus N, Denmark | ||
| 593 | |a Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, (C-1428-EHA) Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a GENE EXPRESSION PRINCIPAL COMPONENTS |
| 690 | 1 | 0 | |a HEAT KNOCKDOWN RESISTANCE |
| 690 | 1 | 0 | |a QUANTITATIVE POLYMERASE CHAIN REACTION |
| 690 | 1 | 0 | |a QUANTITATIVE TRAIT LOCI |
| 690 | 1 | 0 | |a RECOMBINANT INBRED LINES |
| 690 | 1 | 0 | |a DROSOPHILA PROTEIN |
| 690 | 1 | 0 | |a CHROMOSOME |
| 690 | 1 | 0 | |a FLY |
| 690 | 1 | 0 | |a GENE EXPRESSION |
| 690 | 1 | 0 | |a POLYMERASE CHAIN REACTION |
| 690 | 1 | 0 | |a TEMPERATURE TOLERANCE |
| 690 | 1 | 0 | |a ANIMAL |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a CHEMISTRY |
| 690 | 1 | 0 | |a DROSOPHILA MELANOGASTER |
| 690 | 1 | 0 | |a FEMALE |
| 690 | 1 | 0 | |a GENE EXPRESSION REGULATION |
| 690 | 1 | 0 | |a GENETICS |
| 690 | 1 | 0 | |a HEAT |
| 690 | 1 | 0 | |a MALE |
| 690 | 1 | 0 | |a METABOLISM |
| 690 | 1 | 0 | |a QUANTITATIVE TRAIT LOCUS |
| 690 | 1 | 0 | |a ANIMALS |
| 690 | 1 | 0 | |a DROSOPHILA MELANOGASTER |
| 690 | 1 | 0 | |a DROSOPHILA PROTEINS |
| 690 | 1 | 0 | |a FEMALE |
| 690 | 1 | 0 | |a GENE EXPRESSION REGULATION |
| 690 | 1 | 0 | |a HOT TEMPERATURE |
| 690 | 1 | 0 | |a MALE |
| 690 | 1 | 0 | |a QUANTITATIVE TRAIT LOCI |
| 690 | 1 | 0 | |a DROSOPHILA MELANOGASTER |
| 700 | 1 | |a Larsen, P.F. | |
| 700 | 1 | |a Liu, Y. | |
| 700 | 1 | |a Loeschcke, V. | |
| 773 | 0 | |d 2009 |g v. 55 |h pp. 1050-1057 |k n. 11 |p J. Insect Physiol. |x 00221910 |w (AR-BaUEN)CENRE-668 |t Journal of Insect Physiology | |
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| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_00221910_v55_n11_p1050_Norry |y Handle |
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