Differentiation of human embryonic stem cells into cardiomyocytes
Background: The role of stem cells in the treatment of several conditions, especially heart diseases, is under permanent investigation. Human embryonic stem cells have been successfully differentiated in vitro into cardiomyocytes. Methods of cell culture and cardiomyocyte differentiation are well es...
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| Otros Autores: | , , , , , , , , |
| Formato: | Capítulo de libro |
| Lenguaje: | Español |
| Publicado: |
2009
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| Acceso en línea: | Registro en Scopus Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| LEADER | 06594caa a22008297a 4500 | ||
|---|---|---|---|
| 001 | PAPER-8358 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203810.0 | ||
| 008 | 190411s2009 xx ||||fo|||| 00| 0 spa|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-77952366332 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a RACDA | ||
| 100 | 1 | |a Scassa, M.E. | |
| 245 | 1 | 0 | |a Differentiation of human embryonic stem cells into cardiomyocytes |
| 246 | 3 | 1 | |a Diferenciación en cardiomiocitos a partir de células madre embrionarias humanas |
| 260 | |c 2009 | ||
| 270 | 1 | 0 | |m Miriuka, S. G.; Instituto FLENI, Departamento de Cardiología, Montañeses 2325, C1428AQK Ciudad Autónoma de Buenos Aires, Argentina |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Evans, M.J., Kaufman, M.H., Establishment in culture of pluripotential cells from mouse embryos (1981) Nature, 292, pp. 154-156 | ||
| 504 | |a Thomson, J.A., Itskovitz-Eldor, J., Shapiro, S.S., Waknitz, M.A., Swiergiel, J.J., Marshall, V.S., Embryonic stem cell lines derived from human blastocysts (1998) Science, 282, pp. 1145-1147 | ||
| 504 | |a Kehat, I., Kenyagin-Karsenti, D., Snir, M., Segev, H., Amit, M., Gepstein, A., Human embryonic stem cells can differentiate into myocytes with structural and functional properties of cardiomyocytes (2001) J Clin Invest, 108, pp. 407-14 | ||
| 504 | |a Leor, J., Human embryonic stem cell transplantation to repair the infarcted myocardium (2007) Heart, 93 (10), pp. 1278-84 | ||
| 504 | |a Laflamme, M.A., Murry, C.E., Regenerating the heart (2005) Nat Biotechnol, 23, pp. 845-56 | ||
| 504 | |a Kolossov, E., Lu, Z., Drobinskaya, I., Gassanov, N., Duan, Y., Sauer, H., Identification and characterization of embryonic stem cell-derived pacemaker and atrial cardiomyocytes (2005) FASEB J, 19, pp. 577-579 | ||
| 504 | |a Adewumi, O., Aflatoonian, B., Ahrlund-Richter, L., Amit, M., Andrews, P.W., Beighton, Characterization of human embryonic stem cell lines by the International Stem Cell Initiative (2007) Nat Biotechnol, 25, pp. 803-16. , International Stem Cell Initiative | ||
| 504 | |a Takahashi, K., Yamanaka, S., Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors (2006) Cell, 126 (4), pp. 663-76 | ||
| 504 | |a Takahashi, K., Yamanaka, S., Induction of pluripotent stem cells from mouse embryonic and adult human fibroblasts by defined factors (2006) Cell, 131, pp. 663-76 | ||
| 504 | |a Yu, J., Vodyanik, M.A., Smuga-Otto, K., Antosiewicz-Bourget, J., Frane, J.L., Tian, S., Induced pluripotent stem cell lines derived from human somatic cells (2007) Science, 318, pp. 1917-20 | ||
| 504 | |a Hanna, J., Wernig, M., Markoulaki, S., Sun, C.W., Meissner, A., Cassady, J.P., Treatment of sickle cell anemia mouse model with iPS cells generated from autologous skin (2007) Science, 318, pp. 1920-1923 | ||
| 504 | |a Park, I.H., Arora, N., Huo, H., Maherali, N., Ahfeldt, T., Shimamura, A., Disease-specific induced pluripotent stem cells (2008) Cell, 134, pp. 877-86 | ||
| 520 | 3 | |a Background: The role of stem cells in the treatment of several conditions, especially heart diseases, is under permanent investigation. Human embryonic stem cells have been successfully differentiated in vitro into cardiomyocytes. Methods of cell culture and cardiomyocyte differentiation are well established; signals regulating cardiogenesis have been identified and the cardiomyocytes generated have been used in models of myocardial regeneration. However, several questions still remain and are currently under active investigation. Objective: To develop a culture system that is suitable for the induction of embryonic stem cells to cardiomyocyte differentiation. Material and Methods: Four human embryonic stem cell lines were used. The cells were cultured and differentiation was induced using methods previously described. The presence of cells in an undifferentiated state and cardiomyocyte differentiation was detected by immunohistochemical studies (fluorescent staining) and RT-PCR. Results: The methodology used allowed stem cells growth in the culture, and maintained them in an undifferentiated state. Cardiomyocyte differentiation was achieved in the four cell lines used, yet with uneven efficacy. This was confirmed by the expression of myocardial transcription factors and heart structural proteins. Conclusions: Our system allowed human embryonic stem cell growth and differentiation in the culture. These preliminary results encourage us to continue developing our methods with induced pluripotent stem cells. |l eng | |
| 593 | |a Laboratorio de Biología del Desarrollo Celular, FLENI | ||
| 593 | |a Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires | ||
| 593 | |a Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) | ||
| 593 | |a Departamento de Cardiología, FLENI | ||
| 690 | 1 | 0 | |a CARDIOMYOCYTE |
| 690 | 1 | 0 | |a EMBRYONIC STEM CELLS |
| 690 | 1 | 0 | |a MYOGENESIS |
| 690 | 1 | 0 | |a STRUCTURAL PROTEIN |
| 690 | 1 | 0 | |a TRANSCRIPTION FACTOR |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a CELL CULTURE |
| 690 | 1 | 0 | |a CELL DIFFERENTIATION |
| 690 | 1 | 0 | |a CELL GROWTH |
| 690 | 1 | 0 | |a EMBRYONIC STEM CELL |
| 690 | 1 | 0 | |a HEART DISEASE |
| 690 | 1 | 0 | |a HEART MUSCLE CELL |
| 690 | 1 | 0 | |a HUMAN |
| 690 | 1 | 0 | |a HUMAN CELL |
| 690 | 1 | 0 | |a IMMUNOHISTOCHEMISTRY |
| 690 | 1 | 0 | |a IN VITRO STUDY |
| 690 | 1 | 0 | |a PLURIPOTENT STEM CELL |
| 690 | 1 | 0 | |a PROTEIN EXPRESSION |
| 690 | 1 | 0 | |a PROTEIN STRUCTURE |
| 690 | 1 | 0 | |a REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION |
| 690 | 1 | 0 | |a SIGNAL TRANSDUCTION |
| 690 | 1 | 0 | |a TISSUE REGENERATION |
| 700 | 1 | |a Fernández Espinosa, D. | |
| 700 | 1 | |a Questa, M. | |
| 700 | 1 | |a Videla Richardson, G. | |
| 700 | 1 | |a Losino, N. | |
| 700 | 1 | |a Luzzani, C. | |
| 700 | 1 | |a Guberman, A.S. | |
| 700 | 1 | |a Grancelli, H.O. | |
| 700 | 1 | |a Sevlever, G. | |
| 700 | 1 | |a Miriuka, S.G. | |
| 773 | 0 | |d 2009 |g v. 77 |h pp. 458-464 |k n. 6 |p Rev. Argent. Cardiol. |x 00347000 |t Revista Argentina de Cardiologia | |
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| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_00347000_v77_n6_p458_Scassa |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00347000_v77_n6_p458_Scassa |y Registro en la Biblioteca Digital |
| 961 | |a paper_00347000_v77_n6_p458_Scassa |b paper |c PE | ||
| 962 | |a info:eu-repo/semantics/article |a info:ar-repo/semantics/artículo |b info:eu-repo/semantics/publishedVersion | ||