Teeth and bones as sources of DNA for genetic diversity and sex identification of Commerson's dolphins (Cephalorhynchus commersonii) from Tierra del Fuego, Argentina

Museum specimens are often the only source of genetic material for many species of cetaceans. Most of the protocols that have been developed to extract DNA from teeth and bone samples involve mechanical or chemical disintegration of the material. An alternative method to the mechanical reduction pro...

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Autor principal: Pimper, L.E
Otros Autores: Remis, M.I, Goodall, R.N.P, Baker, C.S
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 2009
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100 1 |a Pimper, L.E. 
245 1 0 |a Teeth and bones as sources of DNA for genetic diversity and sex identification of Commerson's dolphins (Cephalorhynchus commersonii) from Tierra del Fuego, Argentina 
260 |c 2009 
270 1 0 |m Pimper, L.E.; Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Güiraldes 2160, Buenos Aires, Argentina; email: liditas@ege.fcen.uba.ar 
506 |2 openaire  |e Política editorial 
504 |a Baker, C.S., Flórez-González, L., Abernethy, R.B., Rosenbaum, H.C., Slade, R.W., Capella, J., Mitochondrial DNA variation and maternal gene flow among humpback whales of the southern hemisphere (1998) Marine Mammal Science, 14, pp. 721-737 
504 |a Boom, R., Sol, C.J.A., Cansen, C.L., Wertheim-van Pillen, P.M.E., van der Noorda, J., Rapid and simple method for purification of nucleic acids (1990) Journal of Clinical Microbiology, 28, pp. 495-503 
504 |a Dalebout, M.L., van Helden, A., Van Waerebeek, K., Baker, C.S., Molecular genetic identification of southern hemisphere beaked whales (Cetacea: Ziphiidae) (1998) Molecular Ecology, 7, pp. 687-694 
504 |a Dalebout, M.L., Baker, C.S., Cockroft, V.G., Mead, J.G., Yamada, T.K., A comprehensive molecular taxonomy of beaked whales (Cetacea: Ziphiidae) using a validated mitochondrial and nuclear DNA database (2004) Journal of Heredity, 95, pp. 459-473 
504 |a Excoffier, L, Laval, G, & Schneider, S, 2005, Arlequin version 3.0: An integrated software package for population genetics data analysis. Evolutionary Bioinformatics Online, 1, 47-50; Gilson, A., Syvanen, M., Levine, K., Banks, J., Deer gender determination by polymerase chain reaction: Validation study and application to tissues, bloodstains, and hair forensic samples from California (1998) California Fish and Game, 84, pp. 159-169 
504 |a Goodall, R.N.P., Cameron, I.S., Exploitation of small cetaceans off southern South America (1980) Report of the International Whaling Commission, pp. 445-450. , 30 
504 |a Goodall, R. N. P., Schiavini, A. C. M., & Fermani, C. (1994). Net fisheries and net mortality of small cetaceans off Tierra del Fuego, Argentina. In W. F. Perrin, G. P. Donovan, & J. Barlow (Eds.), Gillnets and cetaceans. Report of the International Whaling Commission (Special Issue 15), 295-306; Goodall, R. N. P., Galeazzi, A. R., Leatherwood, S., Miller, K. W., Cameron, I. S., Kastelein, R. K., et al. (1988). Studies of Commerson's dolphins, Cephalorhynchus commersonii, off Tierra del Fuego, 1976-1984, with a review of information on the species in the South Atlantic. In R. L. Brownell, Jr. & G. P. Donovan (Eds.), The biology of the genus Cephalorhynchus. Report of the International Whaling Commission (Special Issue 9), 3-70; Hall, T.A., BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT (1999) Nucleic Acids Symposium Series, pp. 95-98 
504 |a Hanaoka, Y., Inoue, M., Tsai, T.H., Minaguchi, K., Fundamental and practical study for DNA analysis using tooth as a source of DNA (1995) Nippon Hoigaku Zasshi, 49, pp. 1-10 
504 |a Higgins, D.G., Sharp, P.M., Clustal: A package for performing multiple sequence alignment on a microcomputer (1988) Gene, 73, pp. 237-244 
504 |a Höss, M., Pääbo, S., DNA purification from Pleistocene bones by a silica-based purification method (1993) Nucleic Acids Research, 21, pp. 3913-3914 
504 |a Kemp, B.M., Smith, D.G., Use of bleach to eliminate contaminating DNA from the surface of bones and teeth (2005) Forensic Science International, 154, pp. 53-61 
504 |a Lacépède, B.G.E., (1804) Histoire naturelle des cétacés, , Paris: Chez Plasson 
504 |a Maddison, W. P, & Maddison, D. R, 1992, MacClade Release Version 3.04, Analysis of phylogeny and character evolution. Sunderland, MA: Sinauer Associates; Nei, M., (1987) Molecular evolutionary genetics, , New York: Columbia University Press 
504 |a Pichler, F.B., Baker, C.S., Loss of genetic diversity in the endemic Hector's dolphin due to fisheries-related mortality (2000) Proceedings of the Royal Society of London, Series B, 267, pp. 97-102 
504 |a Pichler, F.B., Olavarría, C., Resolving Chilean dolphin (Cephalorhynchus eutropia, Gray 1846) synonymy by sequencing DNA extracted from teeth of museum specimens (2001) Revista de Biología Marina y Oceanografía, 36 (1), pp. 117-121 
504 |a Pichler, F.B., Dalebout, M.L., Baker, C.S., Non-destructive DNA extraction from sperm whale teeth and scrimshaw (2001) Molecular Ecology Notes, 1, pp. 106-109 
504 |a Pichler, F.B., Robineau, D., Goodall, R.N.P., Meÿer, M.A., Olivarría, C., Baker, C.S., Origin and radiation of Southern Hemisphere coastal dolphins (genus Cephalorhynchus) (2001) Molecular Ecology, 10, pp. 2215-2223 
504 |a Rohland, N., Siedel, H., Hofreiter, M., Nondestructive DNA extraction method for mitochondrial DNA analyses of museum specimens (2004) BioTechniques, 36, pp. 814-821 
504 |a Rosenbaum, H.C., Egan, M.G., Clapham, P.J., Brownell Jr., R.L., Desalle, R., An effective method for isolating DNA from historical specimens of baleen (1997) Molecular Ecology, 6, pp. 677-681 
504 |a Rosenbaum, H.C., Egan, M.G., Clapham, P.J., Brownell Jr., R.L., Malik, S., Brown, M.W., Utility of North Atlantic Right Whale museum specimens for assessing changes in genetic diversity (1837) Conservation Biology, 14 (6). , 2000 
520 3 |a Museum specimens are often the only source of genetic material for many species of cetaceans. Most of the protocols that have been developed to extract DNA from teeth and bone samples involve mechanical or chemical disintegration of the material. An alternative method to the mechanical reduction process is presented here for 212 Commerson's dolphin (Cephalorhynchus commersonii) samples collected in Tierra del Fuego, Argentina, from 1974 to 2004. From the total samples, 82.5% (n = 175) allow for amplification to occur of a minimum length of 200 pb for the mtDNA control region, 66.5% (n = 141) of 400 bp, and 51% (n = 108) of 500 bp. A total of 11 haplotypes were defined from 10 polymorphic sites. From the initial number of samples, 64.6% could be sexed via molecular markers. This method is effective for processing large quantities of degraded samples over a short time period, analysing a representative number of mtDNA haplotypes, and allowing robust estimation of historical genetic diversity and trends over time.  |l eng 
593 |a Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Güiraldes 2160, Buenos Aires, Argentina 
593 |a Museo Acatushún de Aves y Mamíferos Marinos Australes (AMMA), Sarmiento 44, 9410 Ushuaia, Tierra del Fuego, Argentina 
593 |a Centro Austral de Investigaciones Científicas (CADIC), Bernardo Houssay 200, 9410 Ushuaia, Tierra del Fuego, Argentina 
593 |a School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand 
593 |a Marine Mammal Institute, Hatfield Marine Science Center, Oregon State University, 2030 SE Marine Science Drive, Newport, OR 97365, United States 
690 1 0 |a BONES 
690 1 0 |a CEPHALORHYNCHUS COMMERSONII 
690 1 0 |a COMMERSON'S DOLPHIN 
690 1 0 |a DNA EXTRACTION 
690 1 0 |a POPULATION GENETIC ANALYSIS 
690 1 0 |a TEETH 
690 1 0 |a BIODEGRADATION 
690 1 0 |a BIOMARKER 
690 1 0 |a BONE 
690 1 0 |a DNA 
690 1 0 |a DOLPHIN 
690 1 0 |a GENETIC ANALYSIS 
690 1 0 |a POPULATION GENETICS 
690 1 0 |a SEX DETERMINATION 
690 1 0 |a TOOTH 
690 1 0 |a CEPHALORHYNCHUS COMMERSONII 
690 1 0 |a CETACEA 
651 4 |a ARGENTINA 
651 4 |a SOUTH AMERICA 
651 4 |a TIERRA DEL FUEGO [(PRV) ARGENTINA] 
700 1 |a Remis, M.I. 
700 1 |a Goodall, R.N.P. 
700 1 |a Baker, C.S. 
773 0 |d 2009  |g v. 35  |h pp. 330-333  |k n. 3  |p Aquatic Mamm.  |x 01675427  |t Aquatic Mammals 
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