Identification of the product of an Agrobacterium tumefaciens chromosomal virulence gene.
The chvB operon of Agrobacterium tumefaciens is required for bacterial attachment to plant cells and for efficient crown gall tumor formation. As defined by the virulence phenotypes of mutants with transposon insertions mapping in the region, the operon was previously mapped to a 5-kilobase (kb) str...
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| Otros Autores: | , , , , |
| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
1988
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| LEADER | 04784caa a22008057a 4500 | ||
|---|---|---|---|
| 001 | PAPER-804 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203008.0 | ||
| 008 | 190411s1988 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0023966335 | |
| 024 | 7 | |2 cas |a beta galactosidase; glucan, 9012-72-0, 9037-91-6; trypsin, 9002-07-7; beta-1,2-glucan, 9051-99-4; beta-Galactosidase, EC 3.2.1.23, 9051-99-4; beta-Glucans; DNA Transposable Elements; Glucans; Trypsin, EC 3.4.21.4 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 100 | 1 | |a Zorreguieta, A. | |
| 245 | 1 | 0 | |a Identification of the product of an Agrobacterium tumefaciens chromosomal virulence gene. |
| 260 | |c 1988 | ||
| 270 | 1 | 0 | |m Zorreguieta, A. |
| 506 | |2 openaire |e Política editorial | ||
| 520 | 3 | |a The chvB operon of Agrobacterium tumefaciens is required for bacterial attachment to plant cells and for efficient crown gall tumor formation. As defined by the virulence phenotypes of mutants with transposon insertions mapping in the region, the operon was previously mapped to a 5-kilobase (kb) stretch of chromosomal DNA. We report here that the operon is actually about 8.5 kb long and that it contains a 7-kb gene coding for a large membrane protein involved in the synthesis of cyclic beta-1,2-glucan. Mutants with transposon insertions within the 5-kb phenotypically defined operon do not synthesize this functional protein, do not synthesize beta-1,2-glucan, and do not form tumors. However, mutants with insertions that map up to 3.5 kb downstream of the phenotypically defined operon synthesize truncated proteins that are active in beta-1,2-glucan synthesis. These mutants form tumors. The truncated proteins correspond closely in size with the map positions of the insertions, suggesting that the insertions truncate the proteins by translational termination. A plasmid that contains only the phenotypically defined chvB operon also codes for a truncated protein. A fusion product between the protein and beta-galactosidase carried on a Tn3-HoHo1 insertion was observed in one mutant. Partial trypsin digestion of wild-type inner membranes generated truncated proteins that were active in beta-1,2-glucan synthesis, demonstrating that a large portion of the protein is not required for beta-1,2-glucan synthesis. The correlation between beta-1,2-glucan synthesis by the truncated proteins and tumorigenesis strongly implicates the polysaccharide product of this protein in tumor formation. |l eng | |
| 593 | |a Instituto de Investigaciones Bioquímicas Fundación Campomar, Facultad de Ciencias Exactas y Naturales, Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a BETA 1,2 GLUCAN |
| 690 | 1 | 0 | |a BETA GALACTOSIDASE |
| 690 | 1 | 0 | |a BETA GLUCAN |
| 690 | 1 | 0 | |a BETA-1,2-GLUCAN |
| 690 | 1 | 0 | |a GLUCAN |
| 690 | 1 | 0 | |a TRYPSIN |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a BACTERIAL CHROMOSOME |
| 690 | 1 | 0 | |a BACTERIAL GENE |
| 690 | 1 | 0 | |a BIOSYNTHESIS |
| 690 | 1 | 0 | |a GENETICS |
| 690 | 1 | 0 | |a HIGH PERFORMANCE LIQUID CHROMATOGRAPHY |
| 690 | 1 | 0 | |a MOLECULAR CLONING |
| 690 | 1 | 0 | |a OPERON |
| 690 | 1 | 0 | |a PATHOGENICITY |
| 690 | 1 | 0 | |a PHENOTYPE |
| 690 | 1 | 0 | |a PLASMID |
| 690 | 1 | 0 | |a POLYACRYLAMIDE GEL ELECTROPHORESIS |
| 690 | 1 | 0 | |a RESTRICTION MAPPING |
| 690 | 1 | 0 | |a RHIZOBIUM RADIOBACTER |
| 690 | 1 | 0 | |a TRANSPOSON |
| 690 | 1 | 0 | |a VIRULENCE |
| 690 | 1 | 0 | |a BETA-GALACTOSIDASE |
| 690 | 1 | 0 | |a BETA-GLUCANS |
| 690 | 1 | 0 | |a CHROMATOGRAPHY, HIGH PRESSURE LIQUID |
| 690 | 1 | 0 | |a CHROMOSOMES, BACTERIAL |
| 690 | 1 | 0 | |a CLONING, MOLECULAR |
| 690 | 1 | 0 | |a DNA TRANSPOSABLE ELEMENTS |
| 690 | 1 | 0 | |a ELECTROPHORESIS, POLYACRYLAMIDE GEL |
| 690 | 1 | 0 | |a GENES, BACTERIAL |
| 690 | 1 | 0 | |a GLUCANS |
| 690 | 1 | 0 | |a OPERON |
| 690 | 1 | 0 | |a PHENOTYPE |
| 690 | 1 | 0 | |a PLASMIDS |
| 690 | 1 | 0 | |a RESTRICTION MAPPING |
| 690 | 1 | 0 | |a RHIZOBIUM RADIOBACTER |
| 690 | 1 | 0 | |a TRYPSIN |
| 690 | 1 | 0 | |a VIRULENCE |
| 700 | 1 | |a Geremia, R.A. | |
| 700 | 1 | |a Cavaignac, S. | |
| 700 | 1 | |a Cangelosi, G.A. | |
| 700 | 1 | |a Nester, E.W. | |
| 700 | 1 | |a Ugalde, R.A. | |
| 773 | 0 | |d 1988 |g v. 1 |h pp. 121-127 |k n. 3 |p Mol. Plant Microbe Interact. |x 08940282 |t Molecular plant-microbe interactions : MPMI | |
| 856 | 4 | 1 | |u https://www.scopus.com/inward/record.uri?eid=2-s2.0-0023966335&doi=10.1094%2fMPMI-1-121&partnerID=40&md5=db568480cc04ad35fe8861d95a498051 |y Registro en Scopus |
| 856 | 4 | 0 | |u https://doi.org/10.1094/MPMI-1-121 |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_08940282_v1_n3_p121_Zorreguieta |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08940282_v1_n3_p121_Zorreguieta |y Registro en la Biblioteca Digital |
| 961 | |a paper_08940282_v1_n3_p121_Zorreguieta |b paper |c PE | ||
| 962 | |a info:eu-repo/semantics/article |a info:ar-repo/semantics/artículo |b info:eu-repo/semantics/publishedVersion | ||
| 999 | |c 61757 | ||