A single-step purification of an extracellular fungal laccase

A single-step purification procedure for Neurospora crassa laccase is reported. It used Celite chromatography which permits the concentration of the extracellular enzyme from large culture volumes. This method is useful to isolate any fungal or plant laccase as well as other coppercontaining protein...

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Autor principal: Grotewold, E.
Otros Autores: Taccioli, G.E, Aisemberg, G.O, Judewicz, N.D
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: Kluwer Academic Publishers 1988
Acceso en línea:Registro en Scopus
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100 1 |a Grotewold, E. 
245 1 2 |a A single-step purification of an extracellular fungal laccase 
260 |b Kluwer Academic Publishers  |c 1988 
270 1 0 |m Judewicz, N.D.; Instituto de Investigaciones en Ingenieria Genetica y Biologia Molecular. INGEBI (CONICET), and Facultad de Ciencias Exactas y Naturales (UBA), Vuelta de Obligado 2490, Buenos Aires, 1428, Argentina 
506 |2 openaire  |e Política editorial 
504 |a Aisemberg, G.O., Taccioli, E.G., Grotewold, E., Judewicz, N.D., Differential gene expression promoted by cycloheximide in Neurospora crassa (1987) Experimental Mycology, 11, pp. 122-127 
504 |a Bollag, J.M., Leonowicz, A., Comparative studies of extracellular fungal laccases (1984) Applied and Environmental Microbiology, 48, pp. 849-854 
504 |a Dubernet, A., Ribéreau-Gayon, P., Lerner, H.R., Harel, E., Mayer, A.M., Purification and properties of laccase from Botrytis cinerea (1977) Phytochemistry, 16, pp. 191-193 
504 |a Durrens, P., The phenol oxidases of the ascomycete Podospora anserina: the three forms of the major laccase activity (1981) Archives of Microbiology, 130, pp. 121-124 
504 |a Esser, K., Dick, S., Gielen, W., Die Phenoloxydasen des Ascomyceten Podospora anserina. II. Reinigung und Eigenschaften der Laccase (1964) Archive für Mikrobiologie, 48, pp. 306-318 
504 |a Evans, C.S., Lignin degradation (1987) Process Biochemistry, 22, pp. 102-105 
504 |a Fahraeus, G., Reinhammar, B., Large scale production and purification of laccase from culture of the fungus Polyporus versicolor and some properties of laccase A (1967) Acta Chemica Scandinavica, 21, pp. 2367-2378 
504 |a Froehner, S.C., Eriksson, K.E., Induction of Neurospora crassa laccase with protein synthesis inhibitors (1974) Journal of Bacteriology, 120, pp. 450-457 
504 |a Froehner, S.C., Eriksson, K.E., Purification and properties of Neurospora crassa laccase (1974) Journal of Bacteriology, 120, pp. 458-465 
504 |a Gigi, O., Marbach, I., Mayer, A.M., Induction of laccase formation in Botrytis (1980) Phytochemistry, 19, pp. 2273-2275 
504 |a Gigi, O., Marbach, I., Mayer, A.M., Properties of gallic acid-induced extracellular laccase of Botrytis cinerea (1981) Phytochemistry, 20, pp. 1211-1213 
504 |a Horowitz, N.H., Feldman, H.M., Pall, M.L., Derepression of tyrosinase synthesis in Neurospora by cycloheximide, actinomycin D. and puromycin (1970) Journal of Biological Chemistry, 245, pp. 2784-2788 
504 |a Katan, T., Galun, E., A rapid and efficient method for the purification of tyrosinase from Neurospora (1975) Analytical Biochemistry, 67, pp. 485-492 
504 |a Kirk, T.K., Farrell, R.L., Enzymatic ‘combution’: the microbial degradation of lignin (1987) Annual Review of Microbiology, 41, pp. 465-505 
504 |a Kurtz, M.B., Champe, S.P., Purification and characterization of the conidial laccase of Aspergillus nidulans (1982) Journal of Bacteriology, 151, pp. 1338-1345 
504 |a Lerch, K., Deinum, J., Reinhammar, B., The state of copper in Neurospora laccase (1978) Biochimica et Biophysica Acta, 534, pp. 7-14 
504 |a Marbach, I., Harel, E., Mayer, A.M., Inducer and culture medium dependent properties of extracellular laccase from Botrytis cinerea (1983) Phytochemistry, 22, pp. 1535-1538 
504 |a Marbach, I., Harel, E., Mayer, A.M., Molecular properties of extracellular Botrytis cinerea laccase (1984) Phytochemistry, 23, pp. 2713-2717 
504 |a Mayer, A.M., Harel, E., Polyphenoloxidases in plants (1979) Phytochemistry, 18, pp. 193-215 
504 |a Mosbach, R., Purification and some properties of laccase from Polyporus versicolor (1963) Biochimica et Biophysica Acta, 73, pp. 204-212 
504 |a Scott, W.A., Biochemical genetics of morphogenesis in Neurospora (1975) Annual Review of Microbiology, 30, pp. 85-104 
504 |a Vogel, H.J., A convenient growth medium for Neurospora (Medium N) (1956) Microbiology and Genetics Bulletin, 13, pp. 42-43 
504 |a Ward, E.W.B., Stoessl, A., Post-infectional inhibitions from plants. III. Detoxification of capsidiol, an antifungal compound from peppers (1972) Phytopathology, 62, pp. 1186-1187 
520 3 |a A single-step purification procedure for Neurospora crassa laccase is reported. It used Celite chromatography which permits the concentration of the extracellular enzyme from large culture volumes. This method is useful to isolate any fungal or plant laccase as well as other coppercontaining proteins. This work also takes advantage of the 100-fold induction of the enzyme by low concentrations of cycloheximide. © 1988 Oxford University Press.  |l eng 
593 |a Instituto de Investigaciones en Ingenieria Genetica y Biologia Molecular. INGEBI (CONICET), and Facultad de Ciencias Exactas y Naturales (UBA), Vuelta de Obligado 2490, Buenos Aires, 1428, Argentina 
700 1 |a Taccioli, G.E. 
700 1 |a Aisemberg, G.O. 
700 1 |a Judewicz, N.D. 
773 0 |d Kluwer Academic Publishers, 1988  |g v. 4  |h pp. 357-363  |k n. 3  |p Mircen Journal  |x 02650762  |t Mircen Journal of Applied Microbiology and Biotechnology 
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