Effect of estradiol and tamoxifen on the anchorage-independent growth of the subpopulations derived from MCF-7 breast carcinoma cells: Cytogenetic analysis of the stem cell subpopulation

The MCF-7 breast carcinoma cell line can be separated by Percoll density gradient centrifugation into several subpopulations, A to F, one of which (E) has been previously suggested to be highly enriched in stem cells. The anchorage-independent growth of the different fractions and its sensitivity to...

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Autor principal: Podhajcer, O.L
Otros Autores: Resnicoff, M., Bover, L., Medrano, E.E, Slavutsky, I., Larripa, I., Mordoh, J.
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 1988
Acceso en línea:Registro en Scopus
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024 7 |2 scopus  |a 2-s2.0-0023700205 
024 7 |2 cas  |a estradiol, 50-28-2; tamoxifen, 10540-29-1; Estradiol, 50-28-2; Genetic Markers; Tamoxifen, 10540-29-1 
040 |a Scopus  |b spa  |c AR-BaUEN  |d AR-BaUEN 
030 |a ECREA 
100 1 |a Podhajcer, O.L. 
245 1 0 |a Effect of estradiol and tamoxifen on the anchorage-independent growth of the subpopulations derived from MCF-7 breast carcinoma cells: Cytogenetic analysis of the stem cell subpopulation 
260 |c 1988 
270 1 0 |m Mordoh, J.; Instituto de Investigaciones Bioquímicas Fundación Campomar, Antonio Machado 151, 1405 Buenos Aires, Argentina 
506 |2 openaire  |e Política editorial 
504 |a Buick, Pollack, (1984) Cancer Res, 44, pp. 4909-4918 
504 |a Resnicoff, Medrano, Podhajcer, Bravo, Bover, Mordoh, (1987) Proc. Natl. Acad. Sci. USA, 84, pp. 7295-7299 
504 |a Suele, Vazquez, Long, Alberty, Brennan, (1973) J. Natl. Cancer Inst, 51, pp. 1409-1415 
504 |a Barkley Butter, Berlinski, Hillman, Kelsey, Toenniges, (1986) Cancer Res, 46, pp. 6339-6348 
504 |a Yang, Soule, McGrath, (1977) J. Natl. Cancer Inst, 59, pp. 1357-1367 
504 |a Shin, Freedman, Risser, Pollack, (1975) Proc. Natl. Acad. Sci. USA, 72, pp. 4435-4439 
504 |a Kahn, Shin, (1979) J. Cell Biol, 82, pp. 1-16 
504 |a Smith, (1979) J. Natl. Cancer Inst, 62, pp. 225-230 
504 |a Hamburger, Salmon, (1977) Science, 197, pp. 461-463 
504 |a Seabright, (1971) Lancet, 2, pp. 971-972 
504 |a ISCN, An International System for Human Cytogenetic Nomenclature (1978) (1978) Birth Defects: Original Article Series, 14. , The National Foundation, New York, No. 8 
504 |a ISCN, (1978) Cytogenetic and Genome Research, 21, pp. 309-404. , also in 
504 |a Medrano, E. E., Resnicoff, M., Larcher, F., Cafferata, E. G. A., Podhajcer, O., Bover, L., and Molinari, B. Submitted for publication; Whang-Peng, Lee, Kao-Shan, Seibert, Lippman, (1983) J. Natl. Cancer Inst, 71, pp. 687-695 
504 |a Human Gene Mapping 8 (1985) Eight International Workshop on Human Gene Mapping, 40, pp. 1-4. , 2nd ed., Cytogenet. Cell Genet 
504 |a Podhajcer, Bravo, Sorin, Guman, Cerdeiro, Mordoh, (1986) Cancer, 58, pp. 720-729 
520 3 |a The MCF-7 breast carcinoma cell line can be separated by Percoll density gradient centrifugation into several subpopulations, A to F, one of which (E) has been previously suggested to be highly enriched in stem cells. The anchorage-independent growth of the different fractions and its sensitivity to estradiol (E2) and tamoxifen (TAM) was assayed. The anchorage-independent growth capacity of the different fractions was E>A>B> D>C,F. The E fraction had the highest clonogenic index (6.62 ± 1.18) and was unaffected by E2 or TAM. The karyotypic analysis of the E fraction revealed features similar to those of the unfractionated cell line. It is suggested that the high growth rate of fraction E is due to an enrichment in stem cells and not to the existence of a different clone. © 1988.  |l eng 
536 |a Detalles de la financiación: Universidad de Buenos Aires 
536 |a Detalles de la financiación: Universidad Nacional del Centro de la Provincia de Buenos Aires 
536 |a Detalles de la financiación: Centro de Investigación en Computación 
536 |a Detalles de la financiación: This work has been supported by Grants from the Consejo National de Investigaciones Cientificas y Tecnicas (CONICET), the University of Buenos Aires and the Comisi6n de Investigaciones Cientilicas de la Provincia de Buenos Aires (CIC). L. B., E. E. M., I. L., I. S. and J. M. are members of the CONICET, 0. L. P. is member of the CIC, and M. R. is a Fellow of the Fundaci6n Maria Calderon de la Barca. 
593 |a Hospital Mariano R. Castex, Balcarce 900, 1650 San Martin, Provincia de Buenos Aires, Argentina 
593 |a Instituto de Investigaciones Bioquímicas Fundación Campomar, Antonio Machado 151, 1405 Buenos Aires, Argentina 
593 |a Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Buenos Aires, Argentina 
593 |a Academia Nacional de Medicina, Coronel Diaz, 2497, 1425 Buenos Aires, Argentina 
690 1 0 |a ESTRADIOL 
690 1 0 |a TAMOXIFEN 
690 1 0 |a ANCHORAGE INDEPENDENT GROWTH 
690 1 0 |a CELL CULTURE 
690 1 0 |a CELL GROWTH 
690 1 0 |a CELL STRAIN MCF 7 
690 1 0 |a CONTROLLED STUDY 
690 1 0 |a CYTOLOGY 
690 1 0 |a FEMALE 
690 1 0 |a HUMAN 
690 1 0 |a HUMAN CELL 
690 1 0 |a KARYOTYPING 
690 1 0 |a PRIORITY JOURNAL 
690 1 0 |a STEM CELL 
690 1 0 |a ULTRASTRUCTURE 
690 1 0 |a BREAST NEOPLASMS 
690 1 0 |a CELL ADHESION 
690 1 0 |a CELL DIVISION 
690 1 0 |a CELL LINE 
690 1 0 |a ESTRADIOL 
690 1 0 |a GENETIC MARKERS 
690 1 0 |a HUMAN 
690 1 0 |a KARYOTYPING 
690 1 0 |a SUPPORT, NON-U.S. GOV'T 
690 1 0 |a TAMOXIFEN 
690 1 0 |a TUMOR STEM CELLS 
700 1 |a Resnicoff, M. 
700 1 |a Bover, L. 
700 1 |a Medrano, E.E. 
700 1 |a Slavutsky, I. 
700 1 |a Larripa, I. 
700 1 |a Mordoh, J. 
773 0 |d 1988  |g v. 179  |h pp. 58-64  |k n. 1  |p Exp. Cell Res.  |x 00144827  |w (AR-BaUEN)CENRE-797  |t Experimental Cell Research 
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856 4 0 |u https://doi.org/10.1016/0014-4827(88)90348-5  |y DOI 
856 4 0 |u https://hdl.handle.net/20.500.12110/paper_00144827_v179_n1_p58_Podhajcer  |y Handle 
856 4 0 |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00144827_v179_n1_p58_Podhajcer  |y Registro en la Biblioteca Digital 
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