cAMP regulation of phenobarbital-mediated induction of delta-aminolevulinate synthase mRNA in hepatocytes from normal and experimental diabetic rats.

We examined the mechanism underlying the effect of cAMP on delta-aminolevulinate synthase mRNA biosynthesis in isolated hepatocytes from normal and experimental diabetic rats. We have demonstrated that the potentiation by dibutyryl cAMP of the phenobarbital-mediated induction of delta-aminolevulinat...

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Detalles Bibliográficos
Autor principal: Varone, C.L
Otros Autores: Canépa, E.T, Llambías, E.B, Grinstein, M.
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 1994
Acceso en línea:Registro en Scopus
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Registro en la Biblioteca Digital
Aporte de:Registro referencial: Solicitar el recurso aquí
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008 190411s1994 xx ||||fo|||| 00| 0 eng|d
024 7 |2 scopus  |a 2-s2.0-0028508667 
024 7 |2 cas  |a 5 aminolevulinate synthase, 9037-14-3; bucladesine, 16980-89-5, 362-74-3; cyclic AMP, 60-92-4; phenobarbital, 50-06-6, 57-30-7, 8028-68-0; 5-Aminolevulinate Synthetase, EC 2.3.1.37; Bucladesine, 362-74-3, 50-06-6; Cyclic AMP, 60-92-4; Phenobarbital, 50-06-6; RNA, Messenger 
040 |a Scopus  |b spa  |c AR-BaUEN  |d AR-BaUEN 
100 1 |a Varone, C.L. 
245 1 0 |a cAMP regulation of phenobarbital-mediated induction of delta-aminolevulinate synthase mRNA in hepatocytes from normal and experimental diabetic rats. 
260 |c 1994 
270 1 0 |m Varone, C.L. 
506 |2 openaire  |e Política editorial 
520 3 |a We examined the mechanism underlying the effect of cAMP on delta-aminolevulinate synthase mRNA biosynthesis in isolated hepatocytes from normal and experimental diabetic rats. We have demonstrated that the potentiation by dibutyryl cAMP of the phenobarbital-mediated induction of delta-aminolevulinate synthase enzyme activity, observed in our previously reported studies, reflects an increased amount of its mRNA. The inducing effect exerted by phenobarbital on the biosynthesis of delta-aminolevulinate synthase mRNA in diabetic hepatocytes is greater than that observed in normal cells. This enhanced response to the increased level of endogenous cAMP in diabetic hepatocytes is apparently sufficient for a maximum activation of the cAMP-dependent protein kinase. The present results suggest that in rat liver dibutyryl cAMP modulates delta-aminolevulinate synthase mRNA biosynthesis by acting predominantly, if not exclusively, at the level of gene transcription.  |l eng 
593 |a Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina 
690 1 0 |a 5 AMINOLEVULINATE SYNTHASE 
690 1 0 |a BUCLADESINE 
690 1 0 |a CYCLIC AMP 
690 1 0 |a MESSENGER RNA 
690 1 0 |a PHENOBARBITAL 
690 1 0 |a ANIMAL 
690 1 0 |a ARTICLE 
690 1 0 |a BIOSYNTHESIS 
690 1 0 |a DOSE RESPONSE 
690 1 0 |a EXPERIMENTAL DIABETES MELLITUS 
690 1 0 |a GENE EXPRESSION REGULATION 
690 1 0 |a GENETICS 
690 1 0 |a HALF LIFE TIME 
690 1 0 |a IN VITRO STUDY 
690 1 0 |a LIVER 
690 1 0 |a MALE 
690 1 0 |a METABOLISM 
690 1 0 |a MOLECULAR GENETICS 
690 1 0 |a NORTHERN BLOTTING 
690 1 0 |a NUCLEOTIDE SEQUENCE 
690 1 0 |a RAT 
690 1 0 |a RAT STRAIN 
690 1 0 |a TIME 
690 1 0 |a 5-AMINOLEVULINATE SYNTHETASE 
690 1 0 |a ANIMALS 
690 1 0 |a BASE SEQUENCE 
690 1 0 |a BLOTTING, NORTHERN 
690 1 0 |a BUCLADESINE 
690 1 0 |a CYCLIC AMP 
690 1 0 |a DIABETES MELLITUS, EXPERIMENTAL 
690 1 0 |a DOSE-RESPONSE RELATIONSHIP, DRUG 
690 1 0 |a GENE EXPRESSION REGULATION, ENZYMOLOGIC 
690 1 0 |a HALF-LIFE 
690 1 0 |a LIVER 
690 1 0 |a MALE 
690 1 0 |a MOLECULAR SEQUENCE DATA 
690 1 0 |a PHENOBARBITAL 
690 1 0 |a RATS 
690 1 0 |a RATS, INBRED STRAINS 
690 1 0 |a RNA, MESSENGER 
690 1 0 |a TIME FACTORS 
700 1 |a Canépa, E.T. 
700 1 |a Llambías, E.B. 
700 1 |a Grinstein, M. 
773 0 |d 1994  |g v. 72  |h pp. 381-390  |k n. 9-10  |p Biochem. Cell Biol.  |x 08298211  |w (AR-BaUEN)CENRE-3918  |t Biochemistry and cell biology = Biochimie et biologie cellulaire 
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856 4 0 |u https://doi.org/10.1139/o94-051  |y DOI 
856 4 0 |u https://hdl.handle.net/20.500.12110/paper_08298211_v72_n9-10_p381_Varone  |y Handle 
856 4 0 |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08298211_v72_n9-10_p381_Varone  |y Registro en la Biblioteca Digital 
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