Polyamine biosynthesis in Phytomonas: Biochemical characterisation of a very unstable ornithine decarboxylase
The metabolism of polyamines as well as their functions as growth regulators in plants have been extensively studied for many years. However, almost nothing is known about the biosynthesis and roles of these substances in Phytomonas spp., parasites of several plants. We have used HPLC and electropho...
Guardado en:
| Autor principal: | |
|---|---|
| Otros Autores: | , , , |
| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
2010
|
| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| LEADER | 15721caa a22015857a 4500 | ||
|---|---|---|---|
| 001 | PAPER-7598 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203721.0 | ||
| 008 | 190411s2010 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-84755161369 | |
| 024 | 7 | |2 cas |a agmatine, 306-60-5; arginine, 1119-34-2, 15595-35-4, 7004-12-8, 74-79-3; benzyloxycarbonylleucylleucylleucinal, 133407-82-6; eflornithine, 67037-37-0, 70052-12-9; ornithine, 70-26-8, 7006-33-9; ornithine decarboxylase, 9024-60-6; proteasome, 140879-24-9; putrescine, 110-60-1, 333-93-7; pyridoxal 5 phosphate, 54-47-7; spermidine, 124-20-9, 334-50-9; spermidine synthase, 37277-82-0, 67016-02-8; spermine, 306-67-2, 71-44-3; Ornithine Decarboxylase, 4.1.1.17; Polyamines; Protozoan Proteins; Putrescine, 110-60-1; Spermidine, 124-20-9; Spermine, 71-44-3 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a IJPYB | ||
| 100 | 1 | |a Marcora, M.S. | |
| 245 | 1 | 0 | |a Polyamine biosynthesis in Phytomonas: Biochemical characterisation of a very unstable ornithine decarboxylase |
| 260 | |c 2010 | ||
| 270 | 1 | 0 | |m Carrillo, C.; Fundación Instituto Leloir, IIBBA - CONICET, Patricias Argentinas 435, C1405BWE Buenos Aires, Argentina; email: ccarrillo@leloir.org.ar |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Algranati, I.D., Sánchez, C.P., González, N.S., Polyamines in Trypanosoma cruzi and Leishmania mexicana (1990) The Biology and Chemistry of Polyamines, pp. 137-146. , Oxford University Press, Oxford, S.H. Goldemberg, I.D. Algranati (Eds.) | ||
| 504 | |a Ariyanayagam, M.R., Fairlamb, A.H., Ovothiol and trypanothione as antioxidants in trypanosomatids (2001) Mol. Biochem. Parasitol., 115, pp. 189-198 | ||
| 504 | |a Arteaga-Nieto, P., Villagomez-Castro, J.C., Calvo-Méndez, C.C., López-Romero, E., Partial purification and characterization of ornithine decarboxylase from Entamoeba histolytica (1996) Int. J. Parasitol., 26, pp. 253-260 | ||
| 504 | |a Bacchi, C.J., Nathan, H.C., Hutner, S.H., McCann, P.P., Sjoerdsma, A., Polyamine metabolism: a potential therapeutic target in trypanosomes (1980) Science, 210, pp. 332-334 | ||
| 504 | |a Berlin, C.M., Schimke, R.T., Influence of turnover rates on the responses of enzymes to cortisone (1965) Mol. Pharmacol., 1, pp. 149-156 | ||
| 504 | |a Calvo-Mendez, C., Villagomez-Castro, J.C., López-Romero, E., Ornithine decarboxylase activity in Entamoeba invadens (1993) Int. J. Parasitol., 23, pp. 847-852 | ||
| 504 | |a Canellakis, E.S., Kyriakidis, D.A., Rinehart, C.A., Huang, S.C., Panagiotidis, C., Fong, W.F., Regulation of polyamine biosynthesis by antizyme and some recent developments relating the induction of polyamine biosynthesis to cell growth. Review (1985) Biosci. Rep., 5, pp. 189-204 | ||
| 504 | |a Cánepa, G.E., Carrillo, C., Armesto, A.R., Bouvier, L.A., Miranda, M.R., Pereira, C.A., Phytomonas: transport of amino acids, hexoses and polyamines (2007) Exp. Parasitol., 117, pp. 106-110 | ||
| 504 | |a Carrillo, C., Cejas, S., Gonzalez, N.S., Algranati, I.D., Trypanosoma cruzi epimastigotes lack ornithine decarboxylase but can express a foreign gene encoding this enzyme (1999) FEBS Lett., 454, pp. 192-196 | ||
| 504 | |a Carrillo, C., Cejas, S., Cortés, M.M., Ceriani, C., Huber, M.A., González, N.S., Algranati, I.D., Sensitivity of trypanosomatid protozoa to DFMO and metabolic turnover of ornithine decarboxylase (2000) Biochem. Biophys. Res. Commun., 279, pp. 663-668 | ||
| 504 | |a Carrillo, C., González, N.S., Algranati, I.D., Trypanosoma cruzi as a model system to study the expression of exogenous genes coding for polyamine biosynthetic enzymes. Induction of DFMO resistance in transgenic parasites (2007) Biochim. Biophys. Acta, 1770, pp. 1605-1611 | ||
| 504 | |a Cataldi, A.A., Algranati, I.D., Polyamines and regulation of ornithine biosynthesis in Escherichia coli (1989) J. Bacteriol., 171, pp. 1998-2002 | ||
| 504 | |a Ceriani, C., González, N.S., Algranati, I.D., Ornithine decarboxylase from Crithidia fasciculata is metabolically unstable and resistant to polyamine down-regulation (1992) FEBS Lett., 301, pp. 261-264 | ||
| 504 | |a (1998) A Guide to the Polyamines, , University Press, Oxford, S.S. Cohen (Ed.) | ||
| 504 | |a Dollet, M., Plant diseases caused by flagellate protozoa (Phytomonas) (1984) Annu. Rev. Phytopathol., 22, pp. 115-132 | ||
| 504 | |a Dollet, M., Phloem-restricted trypanosomatids form a clearly characterised monophyletic group among trypanosomatids isolated from plants (2001) Int. J. Parasitol., 31, pp. 459-467 | ||
| 504 | |a Eichler, W., Properties of purified l-ornithine decarboxylase (EC 4.1.1.17) from Tetrahymena thermophila (1989) J. Protozool., 36, pp. 577-582 | ||
| 504 | |a Fairlamb, A.H., Cerami, A., Metabolism and functions of trypanothione in the Kinetoplastida (1992) Annu. Rev. Microbiol., 46, pp. 695-729 | ||
| 504 | |a Fairlamb, A.H., Blackburn, P., Ulrich, P., Chait, B.T., Cerami, A., Trypanothione: a novel bis(glutathionyl)spermidine cofactor for glutathione reductase in trypanosomatids (1985) Science, 227, pp. 1485-1487 | ||
| 504 | |a Gillin, F.D., Reiner, D.S., McCann, P.P., Inhibition of growth of Giardia lamblia by difluoromethylornithine, a specific inhibitor of polyamine biosynthesis (1984) J. Protozool., 31, pp. 161-163 | ||
| 504 | |a González, N.S., Algranati, I.D., Regulation of putrescine uptake in Leishmania mexicana promastigotes (1994) Cell. Mol. Biol., 40, pp. 907-914 | ||
| 504 | |a González, N.S., Sánchez, C.P., Sferco, L., Algranati, I.D., Control of Leishmania mexicana proliferation by modulation of polyamine intracellular levels (1991) Biochem. Biophys. Res. Commun., 180, pp. 797-804 | ||
| 504 | |a González, N.S., Ceriani, C., Algranati, I.D., Differential regulation of putrescine uptake in Trypanosoma cruzi and other trypanosomatids (1992) Biochem. Biophys. Res. Commun., 188, pp. 120-128 | ||
| 504 | |a González, N.S., Huber, M.A., Algranati, I.D., Spermidine is essential for normal proliferation of trypanosomatid protozoa (2001) FEBS Lett., 508, pp. 323-326 | ||
| 504 | |a Hanfrey, C., Sommer, S., Mayer, M.J., Burtin, D., Michael, A.J., Arabidopsis polyamine biosynthesis: absence of ornithine decarboxylase and the mechanism of arginine decarboxylase activity (2001) Plant J., 27, pp. 551-560 | ||
| 504 | |a Hayashi, S., Murakami, Y., Rapid and regulated degradation of ornithine decarboxylase (1995) Biochem. J., 306, pp. 1-10 | ||
| 504 | |a Heby, O., Polyamines and cell cycle (1980) Polyamines in Biomedical Research, pp. 17-34. , Wiley and Sons, New York, J.M. Gaugas (Ed.) | ||
| 504 | |a Heby, O., Luk, G.D., Schindler, J., Polyamine synthesis inhibitors act as both inducers and suppressors of cell differentiation (1987) Inhibition of Polyamine Metabolism. Biological Significance and Basis for New Therapies, pp. 156-186. , Academic Press, Orlando, Florida, P.P. McCann, A.E. Pegg, A. Sjoerdsma (Eds.) | ||
| 504 | |a Heby, O., Persson, L., Rentala, M., Targeting the polyamine biosynthetic enzymes: a promising approach to therapy of African sleeping sickness, Chagas' disease, and leishmaniasis (2007) Amino Acids, 33, pp. 359-366 | ||
| 504 | |a Hunter, K.J., Strobos, C.A., Fairlamb, A.H., Inhibition of polyamine biosynthesis in Crithidia fasciculata by d,l-alpha-difluoromethylornithine and d,l-alpha-difluoromethylarginine (1991) Mol. Biochem. Parasitol., 46, pp. 35-43 | ||
| 504 | |a Igarashi, K., Kashiwagi, K., Polyamines: mysterious modulators of cellular functions (2000) Biochem. Biophys. Res. Commun., 271, pp. 559-564 | ||
| 504 | |a Jankevicius, J.V., Jankevicius, S.I., Campaner, M., Conchon, I., Maeda, L.A., Teixeira, M.M.G., Freymuller, E., Camargo, E.P., Life cycle and culturing of Phytomonas serpens (Gibbs), a trypanosomatid parasite of tomatoes (1989) J. Protozool., 36, pp. 265-271 | ||
| 504 | |a Lee, D.H., Goldberg, A.L., Selective inhibitors of the proteasome-dependent and vacuolar pathways of protein degradation in Saccharomyces cerevisiae (1996) J. Biol. Chem., 271, pp. 27280-27284 | ||
| 504 | |a Lee, D.H., Goldberg, A.L., Proteasome inhibitors: valuable new tools for cell biologists (1998) Trends Cell Biol., 8, pp. 397-403 | ||
| 504 | |a Li, F., Hua, S.B., Wang, C.C., Gottesdiener, K.M., Procyclic Trypanosoma brucei cell lines deficient in ornithine decarboxylase activity (1996) Mol. Biochem. Parasitol., 78, pp. 227-236 | ||
| 504 | |a Matsufuji, S., Matsufuji, T., Miyazaki, Y., Murakami, Y., Atkins, J.F., Gesteland, R.F., Hayashi, S., Autoregulatory frame shifting in decoding mammalian ornithine decarboxylase antizyme (1995) Cell, 80, pp. 51-60 | ||
| 504 | |a Metcalf, B.W., Bey, P., Danzin, C., Jung, M.J., Casara, P., Velvet, J.P., Catalytic irreversible inhibition of mammalian ornithine decarboxylase (1978) J. Am. Chem. Soc., 100, pp. 2551-2553 | ||
| 504 | |a (1998) Polyamine Protocols, , Humana Press, Totowa, New Jersey, D.M.L. Morgan (Ed.) | ||
| 504 | |a Morris, D.R., Pardee, A.B., Multiple pathways of putrescine biosynthesis in Escherichia coli (1966) J. Biol. Chem., 241, pp. 3129-3135 | ||
| 504 | |a Mûller, S., Coombs, G.H., Walter, R.D., Targeting polyamines of parasitic protozoa in chemotherapy (2001) Trends Parasitol., 17, pp. 242-249 | ||
| 504 | |a Murakami, Y., Matsufuji, S., Kameji, T., Hayashi, S., Igarashi, K., Tamura, T., Tanaka, K., Ichihara, A., Ornithine decarboxylase is degraded by the 26S proteasome without ubiquitination (1992) Nature, 360, pp. 597-599 | ||
| 504 | |a Pegg, A.E., Polyamine metabolism and its importance in neoplastic growth and a target for chemotherapy (1988) Cancer Res., 48, pp. 759-774 | ||
| 504 | |a Poulin, R., Lu, L., Ackermann, B., Bey, P., Pegg, A.E., Mechanism of the irreversible inactivation of mouse ornithine decarboxylase by alpha-difluoromethylornithine. Characterization of sequences at the inhibitor and coenzyme binding sites (1992) J. Biol. Chem., 267, pp. 150-158 | ||
| 504 | |a Satishchandran, C., Boyle, S.M., Antagonistic transcriptional regulation of the putrescine biosynthetic enzyme agmatine ureohydrolase by cyclic AMP and agmatine in Escherichia coli (1984) J. Bacteriol., 157, pp. 552-559 | ||
| 504 | |a Smith, T.A., Recent advances in the biochemistry of plant amines (1975) Phytochemistry, 14, pp. 865-890 | ||
| 504 | |a Van Nieuwenhove, S., Schechter, P.J., Declercq, J., Bone, G., Burke, J., Sjoerdsma, A., Treatment of gambiense sleeping sickness in the Sudan with oral DFMO, an inhibitor of ornithine decarboxylase: first field trial (1985) Trans. R. Soc. Trop. Med. Hyg., 79, pp. 692-698 | ||
| 504 | |a Wilkinson, S.R., Meyer, D.J., Kelly, J.M., Biochemical characterization of a trypanosome enzyme with glutathione-dependent peroxidase activity (2000) Biochem. J., 352, pp. 755-761 | ||
| 504 | |a Yanagisawa, H., Suzuki, Y., Corn agmatine iminohydrolase: purification and properties (1981) Plant Physiol., 67, pp. 697-700 | ||
| 520 | 3 | |a The metabolism of polyamines as well as their functions as growth regulators in plants have been extensively studied for many years. However, almost nothing is known about the biosynthesis and roles of these substances in Phytomonas spp., parasites of several plants. We have used HPLC and electrophoretic analyses to investigate the presence and metabolism of polyamines in Phytomonas Jma strain, detecting both putrescine and spermidine but not spermine. Experiments carried out by incubation of intact parasites with labelled ornithine or putrescine showed the formation of radioactive putrescine or spermidine, respectively. These results indicated that Phytomonas Jma can synthesise these polyamines through the action of ornithine decarboxylase (ODC) and spermidine synthase. On the other hand, we could not detect the conversion of arginine to agmatine, suggesting the absence of arginine decarboxylase (ADC) in Phytomonas. However, we cannot ensure the complete absence of this enzymatic activity in the parasite. Phytomonas ODC required pyridoxal 5'-phosphate for maximum activity and was specifically inhibited by α-difluoromethylornithine. The metabolic turnover of the enzyme was very high, with a half-life of 10-15 min, one of the shortest found among all ODC enzymes studied to date. The parasite proteasome seems to be involved in degradation of the enzyme, since Phytomonas ODC can be markedly stabilized by MG-132, a well known proteasome inhibitor. The addition of polyamines to Phytomonas cultures did not decrease ODC activity, strongly suggesting the possible absence of antizyme in this parasite. © 2010 Australian Society for Parasitology Inc. |l eng | |
| 536 | |a Detalles de la financiación: Universidad de Buenos Aires | ||
| 536 | |a Detalles de la financiación: National Research Council | ||
| 536 | |a Detalles de la financiación: We wish to thank Dr. S.H. Goldemberg for helpful discussions and C. Zadikian for technical assistance. This work was partially supported by the National Research Council (Argentina) and the University of Buenos Aires. | ||
| 593 | |a Fundación Instituto Leloir, IIBBA - CONICET, Buenos Aires, Argentina | ||
| 593 | |a Departamento de Química Biológica - FCEN, Universidad de Buenos Aires (UBA), Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a METABOLIC TURNOVER |
| 690 | 1 | 0 | |a MG-132 |
| 690 | 1 | 0 | |a ORNITHINE DECARBOXYLASE |
| 690 | 1 | 0 | |a PHYTOMONAS JMA |
| 690 | 1 | 0 | |a PROTEASOME |
| 690 | 1 | 0 | |a AGMATINE |
| 690 | 1 | 0 | |a ARGININE |
| 690 | 1 | 0 | |a BENZYLOXYCARBONYLLEUCYLLEUCYLLEUCINAL |
| 690 | 1 | 0 | |a EFLORNITHINE |
| 690 | 1 | 0 | |a ORNITHINE |
| 690 | 1 | 0 | |a ORNITHINE DECARBOXYLASE |
| 690 | 1 | 0 | |a POLYAMINE |
| 690 | 1 | 0 | |a PROTEASOME |
| 690 | 1 | 0 | |a PUTRESCINE |
| 690 | 1 | 0 | |a PYRIDOXAL 5 PHOSPHATE |
| 690 | 1 | 0 | |a SPERMIDINE |
| 690 | 1 | 0 | |a SPERMIDINE SYNTHASE |
| 690 | 1 | 0 | |a SPERMINE |
| 690 | 1 | 0 | |a BIOCHEMISTRY |
| 690 | 1 | 0 | |a BIODEGRADATION |
| 690 | 1 | 0 | |a ELECTROKINESIS |
| 690 | 1 | 0 | |a ENZYME ACTIVITY |
| 690 | 1 | 0 | |a EXPERIMENTAL STUDY |
| 690 | 1 | 0 | |a GROWTH REGULATOR |
| 690 | 1 | 0 | |a INHIBITION |
| 690 | 1 | 0 | |a METABOLISM |
| 690 | 1 | 0 | |a PARASITE |
| 690 | 1 | 0 | |a PLANT |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a CONTROLLED STUDY |
| 690 | 1 | 0 | |a ELECTROPHORESIS |
| 690 | 1 | 0 | |a ENZYME ACTIVITY |
| 690 | 1 | 0 | |a ENZYME ANALYSIS |
| 690 | 1 | 0 | |a ENZYME DEGRADATION |
| 690 | 1 | 0 | |a ENZYME STABILITY |
| 690 | 1 | 0 | |a HALF LIFE TIME |
| 690 | 1 | 0 | |a HIGH PERFORMANCE LIQUID CHROMATOGRAPHY |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a PHYTOMONAS |
| 690 | 1 | 0 | |a PHYTOMONAS JMA |
| 690 | 1 | 0 | |a POLYAMINE SYNTHESIS |
| 690 | 1 | 0 | |a SIGNAL TRANSDUCTION |
| 690 | 1 | 0 | |a ENZYME STABILITY |
| 690 | 1 | 0 | |a ORNITHINE DECARBOXYLASE |
| 690 | 1 | 0 | |a POLYAMINES |
| 690 | 1 | 0 | |a PROTOZOAN PROTEINS |
| 690 | 1 | 0 | |a PUTRESCINE |
| 690 | 1 | 0 | |a SPERMIDINE |
| 690 | 1 | 0 | |a SPERMINE |
| 690 | 1 | 0 | |a TRYPANOSOMATINA |
| 690 | 1 | 0 | |a PHYTOMONAS |
| 700 | 1 | |a Cejas, S. | |
| 700 | 1 | |a González, N.S. | |
| 700 | 1 | |a Carrillo, C. | |
| 700 | 1 | |a Algranati, I.D. | |
| 773 | 0 | |d 2010 |g v. 40 |h pp. 1389-1394 |k n. 12 |p Int. J. Parasitol. |x 00207519 |w (AR-BaUEN)CENRE-5208 |t International Journal for Parasitology | |
| 856 | 4 | 1 | |u https://www.scopus.com/inward/record.uri?eid=2-s2.0-84755161369&doi=10.1016%2fj.ijpara.2010.04.003&partnerID=40&md5=8057bf200a58b291d97f30d56142be58 |y Registro en Scopus |
| 856 | 4 | 0 | |u https://doi.org/10.1016/j.ijpara.2010.04.003 |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_00207519_v40_n12_p1389_Marcora |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00207519_v40_n12_p1389_Marcora |y Registro en la Biblioteca Digital |
| 961 | |a paper_00207519_v40_n12_p1389_Marcora |b paper |c PE | ||
| 962 | |a info:eu-repo/semantics/article |a info:ar-repo/semantics/artículo |b info:eu-repo/semantics/publishedVersion | ||
| 999 | |c 68551 | ||