Protein kinase C in Trypanosoma cruzi epimastigote forms: partial purification and characterization
A protein kinase C activity from epimastigote forms of Trypanosoma cruzi was characterized. Cytosolic extracts were chromatographed on DEAE-cellulose columns giving two peaks of kinase activity which were eluted at 0.1 and 0.15 M NaCl. The first activity peak requires Ca2+ and phosphatidylserine for...
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| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
1989
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| LEADER | 08617caa a22010097a 4500 | ||
|---|---|---|---|
| 001 | PAPER-739 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203004.0 | ||
| 008 | 190411s1989 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0024322313 | |
| 024 | 7 | |2 cas |a protein kinase C, 141436-78-4; Calcium, 7440-70-2; Diglycerides; Egtazic Acid, 67-42-5; Histones; Phosphatidylserines; Protein Kinase C, EC 2.7.1.37; Tetradecanoylphorbol Acetate, 16561-29-8 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a MBIPD | ||
| 100 | 1 | |a Gomez, M.L. | |
| 245 | 1 | 0 | |a Protein kinase C in Trypanosoma cruzi epimastigote forms: partial purification and characterization |
| 260 | |c 1989 | ||
| 270 | 1 | 0 | |m Téllez-Iñón, M.T.; Instituto de Investigaciones en Ingenieria Genetica y Biologia Molecular (INGEBI), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Nishizuka, Studies and perspectives of protein kinase C (1986) Science, 233, pp. 305-312 | ||
| 504 | |a Schafer, Bygrave, Matzenauer, Marmé, Identification of a calcium- and phospholipid-dependent protein kinase in plant tissue (1985) FEBS Lett., 187, pp. 25-30 | ||
| 504 | |a Kikkawa, Nishizuka, The role of protein kinase C in transmembrane signaling (1986) Annu. Rev. Cell. Biol., 2, pp. 149-178 | ||
| 504 | |a Rando, Regulation of protein kinase C activity by lipids (1988) FASEB J., 2, pp. 2348-2355 | ||
| 504 | |a Berridge, Inositol trisphosphate and diacylglycerol, two interacting second messengers (1987) Annu. Rev. Biochem., 56, pp. 159-193 | ||
| 504 | |a Oliveira, Antunes, De Mello, Growth of Trypanosoma cruzi epimastigotes controlled by shifts in cyclic AMP mediated by adrenergic ligands (1984) Mol. Biochem. Parasitol., 11, pp. 283-292 | ||
| 504 | |a Rangel-Aldao, Allende, Triana, Piras, Henriquez, Piras, Possible roles of cAMP in the differentiation of Trypanosoma cruzi (1987) Mol. Biochem. Parasitol., 22, pp. 39-43 | ||
| 504 | |a Gonzales-Perdomo, Romero, Goldenberg, Cyclic AMP and adenylate cyclase activators stimulate Trypanosoma cruzi differentiation (1988) Exp. Parasitol., 66, pp. 205-212 | ||
| 504 | |a Ulloa, Mesri, Esteva, Torres, Téllez-Iñón, Cyclic AMP-dependent protein kinase activity in Trypanosoma cruzi (1988) Biochem. J., 255, pp. 319-326 | ||
| 504 | |a Téllez-Iñón, Ulloa, Torruella, Torres, Calmodulin and Ca2+-dependent cyclic AMP. phosphodiesterase activity in Trypanosoma cruzi (1985) Mol. Biochem. Parasitol., 17, pp. 143-153 | ||
| 504 | |a Schenkman, Yoshida, Cardoso de Almedia, Glycophosphatidylinositol-anchored proteins in metacyclic trypomastigotes of Trypanosoma cruzi (1988) Mol. Biochem. Parasitol., 29, pp. 141-152 | ||
| 504 | |a Carvalho, De Souza, Trypanosoma cruzi. Macrophage interaction during treatment with PMA (1986) Mem. Inst. Oswaldo Cruz, p. 71. , (Abstr.) | ||
| 504 | |a Vannier Santos, Saraiva, De Souza, Phorbol ester treatment of Leishmania mexicana amazonensis increases its uptake by macrophages (1988) Mem. Inst. Oswaldo Cruz, 83, p. 36. , (Abstr.) | ||
| 504 | |a Johnson, Walseth, The enzymatic preparation of [32P]ATP, [32P]GTP, [32P]cAMP and [32P]cGMP, and their use in assay of adenylate and guanylate cyclases, and cyclic nucleotide phosphodiesterase (1979) Adv. Cycl. Nucl. Res., 10, pp. 135-167 | ||
| 504 | |a Gupa, Reddy, Randerath, 32P-postlabeling analysis of non-radioactive aromatic carcinogen-DNA adducts (1982) Carcinogenesis, 9, pp. 1081-1092 | ||
| 504 | |a Wynne de Martini, Abramo Orrego, de Rissio, Alvarez, Mujica, Culture of Trypanosoma cruzi in a monophasic medium (1980) Application to a large-scale cultures in fermentation processes, 40, pp. 109-114. , 5th ed., Medicina (B. Aires) | ||
| 504 | |a Walsh, Valentine, Ngai, Carruthers, Hollemberg, Ca2+-dependent hydrophobic interactions chromatography (1984) Isolation of a novel Ca2+-binding protein and protein kinase C from bovine brain, 224, pp. 117-127. , 5th ed., Biochem. J | ||
| 504 | |a Roskoski, Assays of protein kinase (1983) Methods Enzymol., 99, pp. 3-6 | ||
| 504 | |a Cannata, Frasch, Cataldi de Flombaum, Segura, Cazzulo, Two forms of malic enzyme with different regulatory properties in Trypanosoma cruzi (1979) Biochem. J., 184, pp. 409-419 | ||
| 504 | |a Cannata, Valle, Docampo, Cazzulo, Subcellular localization of phosphoenolpyruvate carboxykinase in the trypanosomatids Trypanosoma cruzi and Crithidia fasciculata (1982) Mol. Biochem. Parasitol., 6, pp. 151-160 | ||
| 504 | |a Lowry, Roseborough, Farr, Randall, Protein measurement with the Folin phenol reagent (1951) J. Biol. Chem., 193, pp. 265-275 | ||
| 504 | |a Mazzei, Katon, Kuo, Polymyxin B is a more selective inhibitor for phospholipid-sensitive Ca2+-dependent protein kinase than for calmodulin sensitive Ca2+-dependent protein kinase (1982) Biochem. Biophys. Res. Commun., 109, pp. 1129-1133 | ||
| 504 | |a Tamaoki, Nomoto, Takahashi, Kato, Morimoto, Tomita, Staurosporine, a potent inhibitor of phospholipid/Ca2+-dependent protein kinase (1986) Biochem. Biophys. Res. Commun., 135, pp. 397-402 | ||
| 504 | |a Kikkawa, Takai, Minakuchi, Inohara, Nishizuka, Calcium activated phospholipid dependent protein kinase from rat brain (1982) J. Biol. Chem., 257, pp. 13341-13348 | ||
| 504 | |a Huang, Lesse Chan, Singh, Nakabayashi, Huang, Autophosphorylation of rat brain Ca2+-activated and phospholipid-dependent protein kinase (1986) J. Biol. Chem., 261, pp. 12134-12140 | ||
| 504 | |a Niedel, Kuhn, Vandenbark, Phorbol diester receptor copurifies with protein kinase C. (1983) Proc. Natl. Acad. Sci. USA, 80, pp. 36-40. , 5th ed | ||
| 520 | 3 | |a A protein kinase C activity from epimastigote forms of Trypanosoma cruzi was characterized. Cytosolic extracts were chromatographed on DEAE-cellulose columns giving two peaks of kinase activity which were eluted at 0.1 and 0.15 M NaCl. The first activity peak requires Ca2+ and phosphatidylserine for activity. Further kinase purification was performed by chromatography on phenyl Sepharose columns. In these columns the enzyme activity was adsorbed in the presence of Ca2+ and eluted with a EGTA-containing buffer. T. cruzi protein kinase C activity preferentially phosphorylated histone H1. It was stimulated by diacylglycerol and phorbol myristate acetate, and inhibited by polymyxin B and staurosporine. After subcellular fractionation of epimastigote cells, the kinase was found to be associated with microsomal and cytosolic fractions. © 1989. |l eng | |
| 593 | |a Instituto de Investigaciones en Ingenieria Genetica y Biologia Molecular (INGEBI), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina | ||
| 593 | |a Instituto de Investigaciones Bioquímicas 'Fundación Campomar', Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a DIACYLGLYCEROL |
| 690 | 1 | 0 | |a PHORBOL ESTER |
| 690 | 1 | 0 | |a PROTEIN KINASE C |
| 690 | 1 | 0 | |a STAUROSPORINE |
| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 690 | 1 | 0 | |a PROTEIN KINASE C |
| 690 | 1 | 0 | |a ANIMAL CELL |
| 690 | 1 | 0 | |a ENZYME ACTIVITY |
| 690 | 1 | 0 | |a EPIMASTIGOTE |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a PROTOZOON |
| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 690 | 1 | 0 | |a ANIMAL |
| 690 | 1 | 0 | |a CALCIUM |
| 690 | 1 | 0 | |a CHROMATOGRAPHY, AGAROSE |
| 690 | 1 | 0 | |a CHROMATOGRAPHY, DEAE-CELLULOSE |
| 690 | 1 | 0 | |a CYTOSOL |
| 690 | 1 | 0 | |a DIGLYCERIDES |
| 690 | 1 | 0 | |a EGTAZIC ACID |
| 690 | 1 | 0 | |a HISTONES |
| 690 | 1 | 0 | |a MICROSOMES |
| 690 | 1 | 0 | |a PHOSPHATIDYLSERINES |
| 690 | 1 | 0 | |a PHOSPHORYLATION |
| 690 | 1 | 0 | |a PROTEIN KINASE C |
| 690 | 1 | 0 | |a SUPPORT, NON-U.S. GOV'T |
| 690 | 1 | 0 | |a TETRADECANOYLPHORBOL ACETATE |
| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 700 | 1 | |a Erijman, L. | |
| 700 | 1 | |a Arauzo, S. | |
| 700 | 1 | |a Torres, H.N. | |
| 700 | 1 | |a Tellez-Inon, M.T. | |
| 773 | 0 | |d 1989 |g v. 36 |h pp. 101-108 |k n. 2 |p Mol. Biochem. Parasitol. |x 01666851 |w (AR-BaUEN)CENRE-6136 |t Molecular and Biochemical Parasitology | |
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| 856 | 4 | 0 | |u https://doi.org/10.1016/0166-6851(89)90182-5 |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_01666851_v36_n2_p101_Gomez |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01666851_v36_n2_p101_Gomez |y Registro en la Biblioteca Digital |
| 961 | |a paper_01666851_v36_n2_p101_Gomez |b paper |c PE | ||
| 962 | |a info:eu-repo/semantics/article |a info:ar-repo/semantics/artículo |b info:eu-repo/semantics/publishedVersion | ||
| 963 | |a VARI | ||
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