A model considering light reabsorption processes to correct in vivo chlorophyll fluorescence spectra in apples

Chlorophyll-a contained in the peel of Granny Smith apples emits fluorescence upon excitation with blue light. The observed emission, collected by an external detector and corrected by its spectral response, is still distorted by light reabsorption processes taking place in the fruit skin and differ...

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Autor principal: Ramos, M.E
Otros Autores: Lagorio, M.G
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 2006
Acceso en línea:Registro en Scopus
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024 7 |2 cas  |a chlorophyll, 1406-65-1, 15611-43-5; chlorophyll a, 479-61-8; Chlorophyll, 1406-65-1 
040 |a Scopus  |b spa  |c AR-BaUEN  |d AR-BaUEN 
030 |a PPSHC 
100 1 |a Ramos, M.E. 
245 1 2 |a A model considering light reabsorption processes to correct in vivo chlorophyll fluorescence spectra in apples 
260 |c 2006 
270 1 0 |m Lagorio, M.G.; INQUIMAE/Dpto. de Química Inorgánica, Analítica Y Qca. Física, Facultad de Ciencias Exactas Y Naturales, Pabellón II, C1428EHA, Buenos Aires, Argentina; email: mgl@qi.fcen.uba.ar 
506 |2 openaire  |e Política editorial 
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504 |a Ramos, M.E., Lagorio, M.G., True fluorescence spectra of leaves (2004) Photochem. Photobiol. Sci., 3, pp. 1063-1066 
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520 3 |a Chlorophyll-a contained in the peel of Granny Smith apples emits fluorescence upon excitation with blue light. The observed emission, collected by an external detector and corrected by its spectral response, is still distorted by light reabsorption processes taking place in the fruit skin and differs appreciably from the true spectral distribution of fluorescence emerging from chlorophyll molecules in the biological tissue. Reabsorption processes particularly affect the ratio of fluorescence intensities at 680 nm and at 730 nm. A model to obtain the correct spectral distribution of the emission, from the experimental fluorescence recorded at a fluorometer detector and corrected for the detector spectral sensitivity, is developed in the present work. Measurements of the whole fruit reflectance, the peel transmittance and the flesh reflectance allow the calculation of the reabsorption-corrected spectra. The model is validated by comparing the corrected emission spectra with that obtained for a thin layer of apple-peel-chloroplasts, where no reabsorption takes place. It is recommended to correct distortions in emission spectra of intact fruits due to light reabsorption effects whenever a correlation between the physiological state of the fruit and its fluorescence spectra is investigated. © The Royal Society of Chemistry and Owner Societies 2006.  |l eng 
593 |a INQUIMAE/Dpto. de Química Inorgánica, Analítica Y Qca. Física, Facultad de Ciencias Exactas Y Naturales, Pabellón II, C1428EHA, Buenos Aires, Argentina 
690 1 0 |a CHLOROPHYLL 
690 1 0 |a APPLE 
690 1 0 |a ARTICLE 
690 1 0 |a BLUE LIGHT 
690 1 0 |a CHLOROPLAST 
690 1 0 |a CORRELATION ANALYSIS 
690 1 0 |a FLUORESCENCE 
690 1 0 |a LIGHT ABSORPTION 
690 1 0 |a MEASUREMENT 
690 1 0 |a MODEL 
690 1 0 |a NONHUMAN 
690 1 0 |a PHOTOSYNTHESIS 
690 1 0 |a PRIORITY JOURNAL 
690 1 0 |a SPECTROSCOPY 
690 1 0 |a ABSORPTION 
690 1 0 |a CHLOROPHYLL 
690 1 0 |a FLUORESCENCE 
690 1 0 |a LIGHT 
690 1 0 |a MALUS 
690 1 0 |a MODELS, CHEMICAL 
690 1 0 |a SPECTROMETRY, FLUORESCENCE 
690 1 0 |a MALUS X DOMESTICA 
700 1 |a Lagorio, M.G. 
773 0 |d 2006  |g v. 5  |h pp. 508-512  |k n. 5  |p Photochem. Photobiol. Sci.  |x 1474905X  |t Photochemical and Photobiological Sciences 
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856 4 0 |u https://doi.org/10.1039/b514248b  |y DOI 
856 4 0 |u https://hdl.handle.net/20.500.12110/paper_1474905X_v5_n5_p508_Ramos  |y Handle 
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