Different signaling pathways are involved in cardiomyocyte survival induced by a Trypanosoma cruzi glycoprotein
We have recently reported that Trypanosoma cruzi infection protects cardiomyocytes against apoptosis induced by growth factor deprivation. Cruzipain, a major parasite antigen, reproduced this survival effect by a Bcl-2-dependent mechanism. In this study, we have investigated the molecular mechanisms...
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2006
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| 005 | 20230518203654.0 | ||
| 008 | 190411s2006 xx ||||fo|||| 00| 0 eng|d | ||
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| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
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| 100 | 1 | |a Aoki, M.d.P. | |
| 245 | 1 | 0 | |a Different signaling pathways are involved in cardiomyocyte survival induced by a Trypanosoma cruzi glycoprotein |
| 260 | |c 2006 | ||
| 270 | 1 | 0 | |m Aoki, M.d.P.; CIBICI-CONICET, Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Haya la Torre Medina Allende, CP 5000 Córdoba, Argentina; email: paoki@bioclin.fcq.unc.edu.ar |
| 506 | |2 openaire |e Política editorial | ||
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| 504 | |a Li, C., Ha, T., Kelley, J., Gao, X., Qiu, Y., Kao, R.L., Browder, W., Williams, D.L., Modulating toll-like receptor mediated signaling by (1→3)-beta-d-glucan rapidly induces cardioprotection (2004) Cardiovasc. Res., 61, pp. 538-547 | ||
| 520 | 3 | |a We have recently reported that Trypanosoma cruzi infection protects cardiomyocytes against apoptosis induced by growth factor deprivation. Cruzipain, a major parasite antigen, reproduced this survival effect by a Bcl-2-dependent mechanism. In this study, we have investigated the molecular mechanisms of cruzipain-induced cardiomyocyte protection. Neonatal BALB/c mouse cardiac myocytes were cultured under minimum serum conditions in the presence of cruzipain or T. cruzi (Tulahuen strain). Some cultures were pretreated with the phosphatidylinositol 3-kinase (PI3K) inhibitor Ly294002 or specific inhibitors of the mitogen-activated protein kinase (MAPK) family members such as the mitogen-activated protein kinase kinase (MEK1) inhibitor PD098059, Jun N-terminal kinase (JNK) inhibitor SP600125, p38 MAPK inhibitor SB203580. Inhibition of PI3K and MEK1 but not JNK or p38 MAPK increased the apoptotic rate of cardiomyocytes treated with cruzipain. Phosphorylation of Akt, a major target of PI3K, and ERK1/2, MEK1-targets, was achieved at 15 min and 5 min, respectively. In parallel, these kinases were strongly phosphorylated by T. cruzi infection. In cultures treated with cruzipain, cleavage of caspase 3 was considerably diminished after serum starvation; Bcl-2 overexpression was inhibited by PD098059 but not by Ly294002, whereas Bad phosphorylation and Bcl-xL expression were increased and differentially modulated by both inhibitors. The results suggest that cruzipain exerts its anti-apoptotic property in cardiac myocytes at least by PI3K/Akt and MEK1/ERK1/2 signaling pathways. We further identified a differential modulation of Bcl-2 family members by these two signaling pathways. © 2006 Elsevier SAS. All rights reserved. |l eng | |
| 536 | |a Detalles de la financiación: Agencia Nacional de Promoción Científica y Tecnológica | ||
| 536 | |a Detalles de la financiación: Fundación Antorchas | ||
| 536 | |a Detalles de la financiación: Secretaria de Ciencia y Tecnología - Universidad Nacional de Córdoba | ||
| 536 | |a Detalles de la financiación: This work was supported by grants from Agencia Nacional de Promoción Científica y Tecnológica (ANPCYT), Secretacía de Ciencia y Técnica-Universidad Nacional de Córdoba (SECYT-UNC) and Fundación Antorchas. M.P.A., O.A.C. and S.G. are Research Career Investigators from CONICET. T.T., A.V.P. and N.G. thank CONICET, for the fellowships granted. We thank Dr. C. Mas for excellent photographic assistance. | ||
| 593 | |a CIBICI-CONICET, Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Haya la Torre Medina Allende, CP 5000 Córdoba, Argentina | ||
| 593 | |a IFIBYNE-CONICET, Laboratorio de Fisiología y Biología Molecular, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria Pabellon 2, Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a CARDIOMYOCYTE |
| 690 | 1 | 0 | |a MAPKS |
| 690 | 1 | 0 | |a PI3K/AKT |
| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 690 | 1 | 0 | |a 2 (2 AMINO 3 METHOXYPHENYL)CHROMONE |
| 690 | 1 | 0 | |a 2 MORPHOLINO 8 PHENYLCHROMONE |
| 690 | 1 | 0 | |a 4 (4 FLUOROPHENYL) 2 (4 METHYLSULFINYLPHENYL) 5 (4 PYRIDYL)IMIDAZOLE |
| 690 | 1 | 0 | |a ANTHRA[1,9 CD]PYRAZOL 6(2H) ONE |
| 690 | 1 | 0 | |a CRUZIPAIN |
| 690 | 1 | 0 | |a GLYCOPROTEIN |
| 690 | 1 | 0 | |a MITOGEN ACTIVATED PROTEIN KINASE |
| 690 | 1 | 0 | |a MITOGEN ACTIVATED PROTEIN KINASE P38 |
| 690 | 1 | 0 | |a PHOSPHATIDYLINOSITOL 3 KINASE |
| 690 | 1 | 0 | |a PROTEIN BAD |
| 690 | 1 | 0 | |a PROTEIN BCL 2 |
| 690 | 1 | 0 | |a PROTEIN BCL XL |
| 690 | 1 | 0 | |a PROTEIN KINASE B |
| 690 | 1 | 0 | |a PROTOZOAL PROTEIN |
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| 690 | 1 | 0 | |a ANIMAL CELL |
| 690 | 1 | 0 | |a ANIMAL MODEL |
| 690 | 1 | 0 | |a APOPTOSIS |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a CELL PROTECTION |
| 690 | 1 | 0 | |a CELL SURVIVAL |
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| 690 | 1 | 0 | |a ENZYME PHOSPHORYLATION |
| 690 | 1 | 0 | |a HEART CELL CULTURE |
| 690 | 1 | 0 | |a HEART MUSCLE CELL |
| 690 | 1 | 0 | |a MOLECULAR MECHANICS |
| 690 | 1 | 0 | |a MOUSE |
| 690 | 1 | 0 | |a NEWBORN |
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| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 690 | 1 | 0 | |a 1-PHOSPHATIDYLINOSITOL 3-KINASE |
| 690 | 1 | 0 | |a ANIMALS |
| 690 | 1 | 0 | |a APOPTOSIS |
| 690 | 1 | 0 | |a BCL-ASSOCIATED DEATH PROTEIN |
| 690 | 1 | 0 | |a BCL-X PROTEIN |
| 690 | 1 | 0 | |a CASPASE 3 |
| 690 | 1 | 0 | |a CASPASES |
| 690 | 1 | 0 | |a CELL SURVIVAL |
| 690 | 1 | 0 | |a CELLS, CULTURED |
| 690 | 1 | 0 | |a CHROMONES |
| 690 | 1 | 0 | |a CYSTEINE ENDOPEPTIDASES |
| 690 | 1 | 0 | |a ENZYME INHIBITORS |
| 690 | 1 | 0 | |a FLAVONOIDS |
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| 690 | 1 | 0 | |a GENES, BCL-2 |
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| 690 | 1 | 0 | |a MICE |
| 690 | 1 | 0 | |a MORPHOLINES |
| 690 | 1 | 0 | |a MYOCYTES, CARDIAC |
| 690 | 1 | 0 | |a P38 MITOGEN-ACTIVATED PROTEIN KINASES |
| 690 | 1 | 0 | |a PHOSPHORYLATION |
| 690 | 1 | 0 | |a PYRIDINES |
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| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
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| 700 | 1 | |a Cano, R.C. | |
| 700 | 1 | |a Pellegrini, A.V. | |
| 700 | 1 | |a Tanos, T. | |
| 700 | 1 | |a Guiñazú, N.L. | |
| 700 | 1 | |a Coso, O.A. | |
| 700 | 1 | |a Gea, S. | |
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