CRF signaling: molecular specificity for drug targeting in the CNS

Corticotrophin-releasing factor (CRF) is the key mediator of the central nervous system response needed to adapt to stress. If adaptation fails, hypersecretion of CRF continues and produces, via CRF type 1 receptors, symptoms pertaining to cognition, appetite, sleep and anxiety, implicating CRF as a...

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Autor principal: Arzt, E.
Otros Autores: Holsboer, F.
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 2006
Acceso en línea:Registro en Scopus
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024 7 |2 cas  |a calcium ion, 14127-61-8; corticotropin releasing factor, 9015-71-8; mitogen activated protein kinase, 142243-02-5; nitric oxide synthase, 125978-95-2; phorbol 13 acetate 12 myristate, 16561-29-8; phospholipase C, 9001-86-9; Corticotropin-Releasing Hormone, 9015-71-8 
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100 1 |a Arzt, E. 
245 1 0 |a CRF signaling: molecular specificity for drug targeting in the CNS 
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270 1 0 |m Arzt, E.; Laboratorio de Fisiología y Biología Molecular, Departamento Fisiología y Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales (FCEN), 1428 Buenos Aires, Argentina 
506 |2 openaire  |e Política editorial 
504 |a Heinrichs, S.C., Koob, G.F., Corticotropin-releasing factor in brain: a role in activation, arousal, and affect regulation (2004) J. Pharmacol. Exp. Ther., 311, pp. 427-440 
504 |a Steckler, T., Holsboer, F., Corticotropin-releasing hormone receptor subtypes and emotion (1999) Biol. Psychiatry, 46, pp. 1480-1508 
504 |a Herman, J.P., Central mechanisms of stress integration: hierarchical circuitry controlling hypothalamo-pituitary-adrenocortical responsiveness (2003) Front. Neuroendocrinol., 24, pp. 151-180 
504 |a Grammatopoulos, D.K., Chrousos, G.P., Functional characteristics of CRH receptors and potential clinical applications of CRH-receptor antagonists (2002) Trends Endocrinol. Metab., 13, pp. 436-444 
504 |a Dautzenberg, F.M., Hauger, R.L., The CRF peptide family and their receptors: yet more partners discovered (2002) Trends Pharmacol. Sci., 23, pp. 71-77 
504 |a Grigoriadis, D.E., The corticotropin-releasing factor receptor: a novel target for the treatment of depression and anxiety-related disorders (2005) Expert Opin. Ther. Targets, 9, pp. 651-684 
504 |a Merchenthaler, I., Immunocytochemical localization of corticotropin-releasing factor (CRF) in the rat brain (1982) Am. J. Anat., 165, pp. 385-396 
504 |a Swanson, L.W., Organization of ovine corticotropin-releasing factor immunoreactive cells and fibers in the rat brain: an immunohistochemical study (1983) Neuroendocrinology, 36, pp. 165-186 
504 |a Bittencourt, J.C., Sawchenko, P.E., Do centrally administered neuropeptides access cognate receptors?: an analysis in the central corticotropin-releasing factor system (2000) J. Neurosci., 20, pp. 1142-1156 
504 |a Chen, Y., Immunocytochemical distribution of corticotropin-releasing hormone receptor type-1 (CRF1)-like immunoreactivity in the mouse brain: light microscopy analysis using an antibody directed against the C-terminus (2000) J. Comp. Neurol., 420, pp. 305-323 
504 |a Chalmers, D.T., Localization of novel corticotropin-releasing factor receptor (CRF2) mRNA expression to specific subcortical nuclei in rat brain: comparison with CRF1 receptor mRNA expression (1995) J. Neurosci., 15, pp. 6340-6350 
504 |a Reul, J.M., Holsboer, F., Corticotropin-releasing factor receptors 1 and 2 in anxiety and depression (2002) Curr. Opin. Pharmacol., 2, pp. 23-33 
504 |a Nemeroff, C.B., Reduced corticotropin releasing factor binding sites in the frontal cortex of suicide victims (1988) Arch. Gen. Psychiatry, 45, pp. 577-579 
504 |a Nemeroff, C.B., Elevated concentrations of CSF corticotropin-releasing factor-like immunoreactivity in depressed patients (1984) Science, 226, pp. 1342-1344 
504 |a Holsboer, F., Blunted corticotropin and normal cortisol response to human corticotropin-releasing factor in depression (1984) N. Engl. J. Med., 311, p. 1127 
504 |a Raadsheer, F.C., Corticotropin-releasing hormone mRNA levels in the paraventricular nucleus of patients with Alzheimer's disease and depression (1995) Am. J. Psychiatry, 152, pp. 1372-1376 
504 |a Zobel, A.W., Effects of the high-affinity corticotropin-releasing hormone receptor 1 antagonist R121919 in major depression: the first 20 patients treated (2000) J. Psychiatr. Res., 34, pp. 171-181 
504 |a Holsboer, F., The rationale for corticotropin-releasing hormone receptor (CRH-R) antagonists to treat depression and anxiety (1999) J. Psychiatr. Res., 33, pp. 181-214 
504 |a Muller, M.B., Holsboer, F., Mice with mutations in the HPA-system as models for symptoms of depression (2006) Biol. Psychiatry, 59, pp. 1104-1115 
504 |a Stenzel-Poore, M.P., Overproduction of corticotropin-releasing factor in transgenic mice: a genetic model of anxiogenic behavior (1994) J. Neurosci., 14, pp. 2579-2584 
504 |a Dirks, A., Overexpression of corticotropin-releasing hormone in transgenic mice and chronic stress-like autonomic and physiological alterations (2002) Eur. J. Neurosci., 16, pp. 1751-1760 
504 |a Smith, G.W., Corticotropin releasing factor receptor 1-deficient mice display decreased anxiety, impaired stress response, and aberrant neuroendocrine development (1998) Neuron, 20, pp. 1093-1102 
504 |a Timpl, P., Impaired stress response and reduced anxiety in mice lacking a functional corticotropin-releasing hormone receptor 1 (1998) Nat. Genet., 19, pp. 162-166 
504 |a Muller, M.B., Limbic corticotropin-releasing hormone receptor 1 mediates anxiety-related behavior and hormonal adaptation to stress (2003) Nat. Neurosci., 6, pp. 1100-1107 
504 |a Philips, A., Novel dimeric Nur77 signaling mechanism in endocrine and lymphoid cells (1997) Mol. Cell. Biol., 17, pp. 5946-5951 
504 |a Zhao, J., Karalis, K.P., Regulation of nuclear factor-κB by corticotropin-releasing hormone in mouse thymocytes (2002) Mol. Endocrinol., 16, pp. 2561-2570 
504 |a Karalis, K.P., NF-κB participates in the corticotropin-releasing, hormone-induced regulation of the pituitary proopiomelanocortin gene (2004) J. Biol. Chem., 279, pp. 10837-10840 
504 |a Lezoualc'h, F., Corticotropin-releasing hormone-mediated neuroprotection against oxidative stress is associated with the increased release of non-amyloidogenic amyloid β precursor protein and with the suppression of nuclear factor-κB (2000) Mol. Endocrinol., 14, pp. 147-159 
504 |a Vila, G., Sonic hedgehog regulates CRH signal transduction in the adult pituitary (2005) FASEB J., 19, pp. 281-283 
504 |a Cao, J., Corticotropin-releasing hormone (CRH) induces vascular endothelial growth factor (VEGF) release from human mast cells via the cAMP/protein kinase A/p38 MAPK pathway (2005) Mol Pharmacol 
504 |a Wang, W., Corticotropin-releasing hormone induces proliferation and TNF-α release in cultured rat microglia via MAP kinase signalling pathways (2003) J. Neurochem., 84, pp. 189-195 
504 |a Kovalovsky, D., Activation and induction of NUR77/NURR1 in corticotrophs by CRH/cAMP: involvement of calcium, protein kinase A, and MAPK pathways (2002) Mol. Endocrinol., 16, pp. 1638-1651 
504 |a Grammatopoulos, D.K., Urocortin, but not corticotropin-releasing hormone (CRH), activates the mitogen-activated protein kinase signal transduction pathway in human pregnant myometrium: an effect mediated via R1α and R2β CRH receptor subtypes and stimulation of Gq-proteins (2000) Mol. Endocrinol., 14, pp. 2076-2091 
504 |a Brar, B.K., Specificity and regulation of extracellularly regulated kinase1/2 phosphorylation through corticotropin-releasing factor (CRF) receptors 1 and 2β by the CRF/urocortin family of peptides (2004) Endocrinology, 145, pp. 1718-1729 
504 |a Rossant, C.J., Corticotropin-releasing factor type 1 and type 2α receptors regulate phosphorylation of calcium/cyclic adenosine 3′,5′-monophosphate response element-binding protein and activation of p42/p44 mitogen-activated protein kinase (1999) Endocrinology, 140, pp. 1525-1536 
504 |a Bayatti, N., Brain region-specific neuroprotective action and signaling of corticotropin-releasing hormone in primary neurons (2003) Endocrinology, 144, pp. 4051-4060 
504 |a Cibelli, G., Corticotropin-releasing factor triggers neurite outgrowth of a catecholaminergic immortalized neuron via cAMP and MAP kinase signalling pathways (2001) Eur. J. Neurosci., 13, pp. 1339-1348 
504 |a Swinny, J.D., Corticotropin-releasing factor and urocortin differentially modulate rat Purkinje cell dendritic outgrowth and differentiation in vitro (2004) Eur. J. Neurosci., 19, pp. 1749-1758 
504 |a Elliott-Hunt, C.R., Potential signalling pathways underlying corticotrophin-releasing hormone-mediated neuroprotection from excitotoxicity in rat hippocampus (2002) J. Neurochem., 80, pp. 416-425 
504 |a Radulovic, M., Corticotropin-releasing factor (CRF) rapidly suppresses apoptosis by acting upstream of the activation of caspases (2003) J. Neurochem., 84, pp. 1074-1085 
504 |a Dermitzaki, E., Corticotropin-releasing hormone induces Fas ligand production and apoptosis in PC12 cells via activation of p38 mitogen-activated protein kinase (2002) J. Biol. Chem., 277, pp. 12280-12287 
504 |a Hoare, S.R., Conformational states of the corticotropin releasing factor 1 (CRF1) receptor: detection, and pharmacological evaluation by peptide ligands (2003) Peptides, 24, pp. 1881-1897 
504 |a Blank, T., Corticotropin-releasing factor receptors couple to multiple G-proteins to activate diverse intracellular signaling pathways in mouse hippocampus: role in neuronal excitability and associative learning (2003) J. Neurosci., 23, pp. 700-707 
504 |a Liu, J.P., A comparative study of the role of adenylate cyclase in the release of adrenocorticotropin from the ovine and rat anterior pituitary (1994) Mol. Cell. Endocrinol., 101, pp. 173-181 
504 |a Pelton, G.H., Repeated stress, like vasopressin, sensitizes the excitatory effects of corticotropin releasing factor on the acoustic startle reflex (1997) Brain Res., 778, pp. 381-387 
504 |a Serradeil-Le Gal, C., An overview of SSR149415, a selective nonpeptide vasopressin V1b receptor antagonist for the treatment of stress-related disorders (2005) CNS Drug Rev., 11, pp. 53-68 
504 |a Kim, E., Sheng, M., PDZ domain proteins of synapses (2004) Nat. Rev. Neurosci., 5, pp. 771-781 
504 |a Kim, J.H., SynGAP: a synaptic RasGAP that associates with the PSD-95/SAP90 protein family (1998) Neuron, 20, pp. 683-691 
504 |a Colledge, M., Targeting of PKA to glutamate receptors through a MAGUK-AKAP complex (2000) Neuron, 27, pp. 107-119 
504 |a Grewal, S.S., Neuronal calcium activates a Rap1 and B-Raf signaling pathway via the cyclic adenosine monophosphate-dependent protein kinase (2000) J. Biol. Chem., 275, pp. 3722-3728 
504 |a Thomas, G.M., Huganir, R.L., MAPK cascade signalling and synaptic plasticity (2004) Nat. Rev. Neurosci., 5, pp. 173-183 
504 |a Menard, C., An essential role for a MEK-C/EBP pathway during growth factor-regulated cortical neurogenesis (2002) Neuron, 36, pp. 597-610 
504 |a Traverse, S., EGF triggers neuronal differentiation of PC12 cells that overexpress the EGF receptor (1994) Curr. Biol., 4, pp. 694-701 
504 |a English, J.D., Sweatt, J.D., A requirement for the mitogen-activated protein kinase cascade in hippocampal long term potentiation (1997) J. Biol. Chem., 272, pp. 19103-19106 
504 |a Selcher, J.C., A necessity for MAP kinase activation in mammalian spatial learning (1999) Learn. Mem., 6, pp. 478-490 
504 |a Schafe, G.E., Activation of ERK/MAP kinase in the amygdala is required for memory consolidation of pavlovian fear conditioning (2000) J. Neurosci., 20, pp. 8177-8187 
504 |a Meller, E., Region-specific effects of acute and repeated restraint stress on the phosphorylation of mitogen-activated protein kinases (2003) Brain Res., 979, pp. 57-64 
504 |a Hao, Y., Mood stabilizer valproate promotes ERK pathway-dependent cortical neuronal growth and neurogenesis (2004) J. Neurosci., 24, pp. 6590-6599 
504 |a Einat, H., The role of the extracellular signal-regulated kinase signaling pathway in mood modulation (2003) J. Neurosci., 23, pp. 7311-7316 
504 |a Refojo, D., Corticotropin-releasing hormone activates ERK1/2 MAPK in specific brain areas (2005) Proc. Natl. Acad. Sci. U. S. A., 102, pp. 6183-6188 
504 |a Cohen, P., Protein kinases-the major drug targets of the twenty-first century? (2002) Nat. Rev. Drug Discov., 1, pp. 309-315 
504 |a Bain, J., The specificities of protein kinase inhibitors: an update (2003) Biochem. J., 371, pp. 199-204 
504 |a Duan, W., Wong, W.S., Targeting mitogen-activated protein kinases for asthma (2006) Curr. Drug Targets, 7, pp. 691-698 
504 |a de Kloet, E.R., Stress and the brain: from adaptation to disease (2005) Nat. Rev. Neurosci., 6, pp. 463-475 
520 3 |a Corticotrophin-releasing factor (CRF) is the key mediator of the central nervous system response needed to adapt to stress. If adaptation fails, hypersecretion of CRF continues and produces, via CRF type 1 receptors, symptoms pertaining to cognition, appetite, sleep and anxiety, implicating CRF as a causal factor in affective disorders. Clinical studies with CRF receptor 1 antagonists support a novel pharmacological strategy for treating stress-related disorders. Here we summarize recent information obtained on CRF receptor 1 signaling and propose the concept of a more focused pharmacological intervention based on the signaling pathways involved. Recent findings suggest that CRF activates, via the same CRF receptor 1, different signaling pathways in specific areas of the brain. This intracellular and neuroanatomical signaling specificity will facilitate the search for less pleiotropic antagonists and new chemical compounds that modulate signal transduction in a site-specific manner. © 2006 Elsevier Ltd. All rights reserved.  |l eng 
536 |a Detalles de la financiación: Agencia Nacional de Promoción Científica y Tecnológica 
536 |a Detalles de la financiación: Universidad de Buenos Aires 
536 |a Detalles de la financiación: Bristol-Myers Squibb Foundation 
536 |a Detalles de la financiación: National Council for Scientific Research 
536 |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas 
536 |a Detalles de la financiación: We thank Damian Refojo for his support in assembling this review. E.A. is a member of the Argentine National Research Council, IFIBYNE, CONICET, Buenos Aires, Argentina. This work was supported by grants from the Max Planck Society, University of Buenos Aires, the Argentine National Research Council (CONICET) and Agencia Nacional de Promoción Científica y Tecnológica-Argentina. Part of this work was supported by the ‘Freedom to Discover Award’ given to F.H. by the Bristol-Myers Squibb Foundation. Affective disorders affective or mood disorders are mental disorders that primarily affect mood, interfere with the activities of daily living, and impair the lives of ∼10–15% of the worldwide population. They are severe stress-related psychiatric disorders and can include suicidal thoughts and suicide attempts. They encompass unipolar depression, bipolar disorder and anxiety disorders. Conditional gene targeting spatial and temporal control of selected transgenes by means of specific promoters and sophisticated recombination systems that enable the overexpression, reduction or deletion of targeted genes. Intracerebroventricular administration a method of delivering small amounts of substances (e.g. antisense oligonucleotides, double-stranded RNA for RNA-mediated interference, hormones, neurotransmitters and/or pharmacological compounds) directly into the cerebral ventricles. Limbic system a complex interconnected network of deep-lying forebrain structures including prefrontal cortex, the limbic lobe (containing the cingulate gyrus, parahippocampal gyrus and hippocampal formation) and other subcortical and diencephalic structures such as the septal area, the nucleus accumbens, the hypothalamus and the amygdala. This circuit manages several processes related to learning and memory, feelings and emotional behavior, including memory processing, emotional association with memory, motivation, judgment, affect and organization of planned actions. MAGUKs membrane-associated guanylate kinases. A family of proteins that function as scaffolds to assemble transmembrane and cytosolic proteins into supramolecular signaling complexes at specific cellular locations. MAPK signaling pathway a three-membered protein kinase signaling pathway that includes the protein kinases MAPKKK, MAPKK and MAPK. MAPK Mitogen-activated protein kinase. Protein kinases that respond to various extracellular signals. The main members of the MAPK family include extracellular-signal-regulated kinases (ERK1/2; also known as p42/p44); c-Jun amino-terminal kinase (JNK); p38 (p38 MAPK); MAPK kinases (MAPKKs), dual serine/tyrosine kinases that activate specific MAPK substrates; MAPK kinase kinase (MAPKKK), kinases (including the Raf family of proteins) that respond to various extracellular signals and elicit signal transduction mechanisms through phosphorylation of MAPKK; MEK1 and MEK2, MAPKKs that are specific ERK kinases; and Ras, a family of small GTP-binding proteins that activate numerous signaling molecules through their regulatory interaction with effectors such as Raf-1 and B-Raf. Neuroprotection a process that facilitates the survival of neuronal cells injured by different insults such as toxicity, infection and inflammatory processes, traumatic brain injury and stroke. NFκB nuclear factor-κB. A transcription factor that regulates the expression of genes involved in several cellular processes such as inflammation, cell proliferation and apoptosis. NOS nitric oxide synthase. An enzyme that catalyzes the formation of nitric oxide, a signaling molecule and a neurotransmitter, from oxygen and arginine. Nur77 orphan nuclear receptor, also known as nerve growth factor inducible B, that belongs to the Nur subfamily of transcription factors. PLC cascade binding of a hormone to its receptor leads to activation of the G protein G q , which in turn activates phosphatidylinositol-specific phospholipase C (PLC) by a mechanism analogous to activation of adenylyl cyclase. PLC hydrolyzes phosphatidylinositol (4,5)-bisphosphate to the second messengers 1,2-diacylglycerol and inositol (1,4,5)-trisphosphate, which facilitate membrane targeting of signaling enzymes through lipid binding and increase intracellular Ca 2+ , respectively. PKA cascade ligand binding to GPCRs activates a stimulatory G-protein (G s ) that interacts with adenylyl cyclase, causing accumulation of cAMP. Active cAMP-dependent protein kinase (PKA) catalytic subunits are released on binding of cAMP to the regulatory subunits. The catalytic subunits phosphorylate targets in the cytoplasm and the nucleus. In addition to PKA, components of the cascade include adenylyl cyclase, an enzyme that synthesizes cAMP on activation by a stimulatory G (G s ) protein; A-kinase anchoring proteins (AKAPs), adaptor proteins that keep PKA at specific cellular locations limiting the targets that can be phosphorylated by activated PKA; cAMP response element binding protein (CREB), the main transcriptional regulator activated by PKA phosphorylation that mediates PKA mechanisms involving gene expression; cAMP, a second messenger synthesized by adenylyl cyclase in response to external stimuli; and forskolin, a pharmacological agent that activates adenylyl cyclase directly, bypassing the need of an activated G s protein. PKC cascade a rise in cytosolic Ca 2+ recruits the serine/threonine protein kinase PKC from the cytosol to the membrane where it is activated by 1,2-diacylglycerol. The activated kinase then phosphorylates various target proteins that control growth and differentiation. Components of the PKC cascade include calmodulin, an intracellular Ca 2+ receptor that initiates various signaling cascades by binding to protein targets in a Ca 2+ -dependent manner; and 1,2-diacylglycerol and inositol (1,4,5)-trisphosphate, second messengers activated in response to a rise in cytosolic Ca 2+ . PMA phorbol-12-myristate-13 acetate. A phorbol ester that activates PKC. Stress the concept of stress was taken by Hans Selye from the physical sciences and defined as ‘the nonspecific response of the body to any demand upon it…’. According to the current concept, stress is a condition in which the homeostatic set points, genetically programmed or learned, do not match with the current or anticipated perception of the internal or external variables sensed. These discrepancies are then evaluated in terms of a real or predicted threat to homeostasis, triggering specific patterns of behavioral and physical adaptive responses. 
593 |a Laboratorio de Fisiología y Biología Molecular, Departamento Fisiología y Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales (FCEN), 1428 Buenos Aires, Argentina 
593 |a Max-Planck Institute of Psychiatry, Kraepelinstrasse 2-10, 80804 Munich, Germany 
690 1 0 |a CALCIUM ION 
690 1 0 |a CORTICOTROPIN RELEASING FACTOR 
690 1 0 |a CORTICOTROPIN RELEASING FACTOR RECEPTOR 1 
690 1 0 |a CYCLIC AMP DEPENDENT PROTEIN KINASE 
690 1 0 |a IMMUNOGLOBULIN ENHANCER BINDING PROTEIN 
690 1 0 |a MEMBRANE ASSOCIATED GUANYLATE CYCLASE KINASE 
690 1 0 |a MITOGEN ACTIVATED PROTEIN KINASE 
690 1 0 |a NITRIC OXIDE SYNTHASE 
690 1 0 |a PHORBOL 13 ACETATE 12 MYRISTATE 
690 1 0 |a PHOSPHOLIPASE C 
690 1 0 |a RECEPTOR SUBTYPE 
690 1 0 |a ADAPTATION 
690 1 0 |a ADDICTION 
690 1 0 |a ANXIETY DISORDER 
690 1 0 |a BRAIN 
690 1 0 |a CELL MEMBRANE PERMEABILITY 
690 1 0 |a CENTRAL NERVOUS SYSTEM 
690 1 0 |a DEPRESSION 
690 1 0 |a DRUG TARGETING 
690 1 0 |a HIPPOCAMPUS 
690 1 0 |a HUMAN 
690 1 0 |a HYPOTHALAMUS HYPOPHYSIS ADRENAL SYSTEM 
690 1 0 |a LIMBIC CORTEX 
690 1 0 |a MENTAL DISEASE 
690 1 0 |a MOOD DISORDER 
690 1 0 |a NEUROANATOMY 
690 1 0 |a NEUROPHARMACOLOGY 
690 1 0 |a NONHUMAN 
690 1 0 |a PRIORITY JOURNAL 
690 1 0 |a REVIEW 
690 1 0 |a SIGNAL TRANSDUCTION 
690 1 0 |a STRESS 
690 1 0 |a ANIMALS 
690 1 0 |a BRAIN 
690 1 0 |a CORTICOTROPIN-RELEASING HORMONE 
690 1 0 |a HUMANS 
690 1 0 |a MENTAL DISORDERS 
690 1 0 |a SIGNAL TRANSDUCTION 
690 1 0 |a STRESS 
690 1 0 |a STRESS, PHYSIOLOGICAL 
700 1 |a Holsboer, F. 
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