Metronidazole-induced alterations in murine spermatozoa morphology
The aim of this work was to assess the effect of metronidazole (MTZ) on the stages of the seminiferous epithelial cycle and spermatozoa morphology when the drug is administered in human therapeutic doses to 60-day-old CFW male mice. The frequency of the stages was established by counting spermatocyt...
Guardado en:
| Autor principal: | |
|---|---|
| Otros Autores: | , , |
| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
2007
|
| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| Sumario: | The aim of this work was to assess the effect of metronidazole (MTZ) on the stages of the seminiferous epithelial cycle and spermatozoa morphology when the drug is administered in human therapeutic doses to 60-day-old CFW male mice. The frequency of the stages was established by counting spermatocytes in pachytene and spermatids. Abnormalities in the flagellum or the head, lack of maturity and multiple malformations, were considered in the morphological analysis. Murine control strain was compared with MTZ treated group (v.ip 130 mg/kg/bw) both kept in standard captivity conditions. Cellular composition or number of stages in the seminiferous tubules were not altered in MTZ exposed animals, though the number of cells in stages I, V and XII was increased. The sperm cell morphology was severely affected by the treatment with potentially serious consequences on the normal fertilization process. Thus, the MTZ has to be considered as a conceivable thread regarding male fertility. © 2006 Elsevier Inc. All rights reserved. |
|---|---|
| Bibliografía: | Freeman, C.D., Klutman, N.E., Lamp, K.C., Metronidazole-a therapeutic review and update (1997) Drugs, 54, pp. 679-708 Mudry, M.D., Carballo, M.A., Labal de Vinuesa, M.D., González Cid, M., Larripa, I., Mutagenic bioassay of certain pharmacological drugs: III. Metronidazole (MTZ) (1994) Mutation Res, 86, pp. 243-277 Mudry, M.D., Gadano, A., González, M., Carballo, M., Mutagénesis química: riesgo y beneficio en el consumo de antiparasitarios (1995) Interciencia, 20 (4), pp. 204-211 Rodriguez Ferreiro, G., Cancino Badías, L., López-Nigro, M., Palermo, A.M., Mudry, M.D., Prieto González, E., DNA Single strand breaks in peripheral blood lymphocytes induced by three nitroimidazole derivatives (2002) Toxicol Lett, 132, pp. 109-115 López Nigro, M., Palermo, A.M., Mudry, M.D., Carballo, M.A., Cytogenetic evaluation of two nitroimidazole derivatives (2003) Toxicol In Vitro, 17, pp. 35-40 Carballo, M.A., Palermo, A.M., Mudry, M.D., Toxicogenetic evaluation of metronidazole in the treatment of women infected with Trichomona vaginalis (2004) Ann Trop Med Parasitol, 98 (2), pp. 139-147 Dobias, L., Cerná, M., Rosner, P., Srám, R., Genotoxicity and carcinogenicity of metronidazole (1994) Mutat Res, 317, pp. 177-194 Mudry, M.D., Martinez Flores, I., Palermo, A.M., Carballo, M.A., Egozcue, J., García-Caldés, M., Embrioletality induced by metronidazole (MTZ) in Rattus norvegicus (2001) Teratogen Carcinogen Mutagen, 21, pp. 197-205 Palermo, A.M., Reynoso, A.S., López Nigro, M., Carballo, M.A., Mudry, M.D., Teratogenic evaluation of metronidazole and ornidazole using Drosophila melanogaster as an experimental model (2004) Birth Defects Res (Part A), 70, pp. 157-162 Diav-Citrin, O., Shechtman, S., Gotteiner, T., Arnon, J., Ornoy, A., Pregnancy outcome after gestational exposure to metronidazole: a prospective controlled cohort study (2001) Teratology, 63 (5), pp. 186-192 Kazy, Z., Puho, E., Czeizel, A.E., Teratogenic potential of vaginal metronidazole treatment during pregnancy (2005) Eur J Obstet Gynecol Reprod Biol, 123 (2), pp. 174-178 Williams, K., McKinnell, C., Saunders, P.T.K., Walker, M., Fisher, J.S., Turner, K.S., Neonatal exposure to potent and environmental oestrogens and abnormalities of the male reproductive system in the rat: evidence for importance of the androgen-oestrogen balance and assessment of the relevance to man (2001) Hum Reprod Update, 7 (3), pp. 236-247 Saradha, B., Mathur, P.P., Effect of environmental contaminants on male reproduction (2006) Environ Toxicol Pharmacol, 21, pp. 34-41 Leblond, C.P., Clermont, Y., Spermiogenesis of mouse, rat, hamster, and guinea pigs revealed by the "periodic acid-fuschsin sulphurus acid" technic (1952) Am J Anat, 90, pp. 167-215 Oakberg, E.F., Duration of spematogenesis in the mouse and timing of stages of the cycle of the seminiferous epithelium (1956) Am J Anat, 99, pp. 319-409 Oakberg, E.F., A description of spermiogenesis in the mouse and its use in an analysis of the cycle of the seminiferous epithelium and germ cell renewal (1956) Am J Anat, 99, pp. 507-516 Sommer, R.J., Ippolito, D.L., Peterson, R.E., In utero and lactation exposure of the male Holtzman rat to 2,3,7,8-tetrachlorodibenzo-p-dioxine: decrease epididymal and ejaculated sperm number without alterations in sperm transit rate (1996) Toxicol Appl Pharmacol, 140, pp. 146-153 Howell, W.M., Black, D., Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method (1980) Experientia, 36, pp. 1014-1015 Wyrobek, A.J., Methods for evaluating the effects of environmental chemicals on human sperm production (1983) Environ Health Perspect, 48, pp. 53-59 Bruce, W.R., Furrer, F., Wyrobek, A.J., Abnormalities in the shape of murine sperm after acute testicular X-radiation (1974) Mutat Res, 23, pp. 381-386 Yang, S., Han, X., Wei, C., Chen, J., Yin, D., The toxic effects of pentaclorophenol on rat Sertoli cells in vitro (2005) Environ Toxicol Pharmacol, 20, pp. 182-187 McClain, R.M., Dowing, J.C., Edgcomb, J.E., Effect of metronidazole on fertility and testicular function in male rats (1989) Fundam Appl Toxicol, 12 (3), pp. 386-396 Grover, J.K., Vats, V., Srinivas, M., Das, S.N., Jha, P., Gupta, D.K., Mitra, D.K., Effect of metronidazole on spermatogenesis and FSH, LH and testosterone levels of pre-pubertal rats (2001) Indian J Exp Biol, 39 (11), pp. 1160-1162 Linder, R.E., Strader, L.F., Slott, V.L., Suarez, J.D., Endpoints of spermatotoxicity in the rat after short duration exposures to fourteen reproductive toxicants (1992) Reprod Toxicol, 6, pp. 491-505 McClain, R.M., Downing, J.C., Edgcomb, J.E., Effect of metronidazole on fertility and testicular function in rats (1989) Fundam Appl Toxicol, 12, pp. 386-396 Joshi, S.C., Mathur, R., Gajraj, A., Sharma, T., Influence of methyl parathion on reproductive parameters in male rats (2003) Environ Toxicol Pharmacol, 14, pp. 91-98 Bustos Obregón, E., González-Hormazabal, P., Mice testicular damage elicited by malathion (2003) Int J Morphol, 21 (2), pp. 155-159 Khan, P.K., Sinha, S.P., Ameliorating effect of vitamin C on murine sperm toxicity induced by three pesticides (endosulfan, phosphamidon and mancozeb) (1996) Mutagenesis, 11 (1), pp. 33-36 Hemavathi, E., Rahiman, M.A., Toxicological effects of ziram, thiram and diethane M-45 assed by sperm shape abnormalities in mice (1993) J Toxicol Environ Health, 38 (4), pp. 393-398 Biswas, S.J., Pathak, S., Khuda-Bukhsh, A.R., Assessment of the genotoxic and cytotoxic potential of anti-epileptic drug, phenobarbital, in mice: a time course study (2004) Mutat Res, 563, pp. 1-11 Hugenholtz, A.P., Bruce, W.R., Radiation induction of mutations affecting sperm morphology in mice (1983) Mutat Res, 107 (1), pp. 177-185 Apostoli, P., Kiss, P., Porru, S., Bonde, J.P., Vanhoorne, M., Male reproductive toxicity of lead in animals and humans (1998) Occup Environ Med, 55 (6), pp. 364-374 Mamina, V.P., Sheiko, L.D., Effects of ionizing radiation and xenobiotics of the spermatogenic epithelium of laboratory animals (2001) Gig Sanit, (6), pp. 24-27 Li, H., Chen, Q., Yao, W., Li, L., Shi, X., Wang, L., Effect of Cr(VI) exposure on sperm quality: human and animal studies (2001) Ann Occup Hyg, 45 (7), pp. 505-511 Ellis, M.K., Richardson, A.G., Foster, J.R., Smith, F.M., Widdowson, P.S., Farnworth, M.J., The reproductive toxicity of molinate and metabolites to the male rat: Effects on testosterone and sperm morphology (2002) Toxicol Appl Pharmacol, 151 (1), pp. 22-32 Dauwe, T., Janssens, E., Kempenaers, B., Eens, M., The effect of heavy metal exposure on egg size, eggshell thickness and the number of spermatozoa in blue tit Parus caeruleus eggs (2004) Environ Pollut, 129, pp. 125-129 Sutkeviciene, N., Andersson, M.A., Zilinskas, H., Andersson, M., Assessment of boar semen quality in relation to fertility with special reference to methanol stress (2005) Theriogenology, 63, pp. 739-747 Guo, C., Lu, Y., Wang Hsu, G., The influence of aluminum exposure on male reproduction and offspring in mice (2005) Environ Toxicol Pharmacol, 20, pp. 135-141 Elizondo, G., Gonsebatt, M.E., Salazar, A.M., Lares, I., Santiago, P., Herrera, J., Genotoxic effects of metronidazole (1996) Mutat Res, 370, pp. 75-80 Fahrig, R., Engelke, M., Reinvestigation of in vivo genotoxicity studies in man. I. No induction of DNA strand breaks in peripheral blood lymphocytes after metronidazole therapy (1997) Mutat Res, 395, pp. 215-221 Foote, R.H., Effects of metronidazole, ipronidazole and dibromochloropropane on rabit and human sperm motility and fertility (2002) Reprod Toxicol, 16, pp. 749-755 Burrel, V.R., Yanagimachi, R., Whitten, W.K., Normal mice develop from oocytes injected with spermatozoa with grossly misshapen heads (1995) Biol Reprod, 52, pp. 709-720 Ali-Hassan, N.H., Miconazole genotoxicity in mice (1997) J Appl Toxicol, 17 (5), pp. 313-319 Otubanjo, O.A., Mosuro, A.A., An in vitro evaluation of abnormal sperm morphology by some anthelmintic drugs in mice (2001) Mutat Res, 497 (1-2), pp. 131-138 Lee, J.D., Kamiguchi, Y., Yanagimachi, R., Analysis of chromosome constitution of human spermatozoa with normal and aberrant head morphologies after injection into mouse oocytes (1996) Hum Reprod, 11, pp. 1942-1946 Kahraman, S., Akarsu, C., Cengiz, G., Dirican, K., Sözen, E., Belgin, C., Fertility of ejaculated and testicular megalohead spermatozoa with intracytoplasmic sperm injection (1999) Hum Reprod, 14, pp. 726-730 Kishikawa, H., Tateno, H., Yanagimachi, R., Chromosome analysis of BALB/c mouse spermatozoa with normal and abnormal head morphology (1999) Biol Reprod, 61 (3), pp. 809-812 Vegetti, W., Van Assche, E., Frias, A., Correlation between sperm parameters and sperm aneuploidy rates investigated by fluorescence in-situ hybridization in infertile men (2000) Hum Reprod, 15, pp. 351-365 Viville, S., Mollard, R., Bach, M.L., Falquet, C., Gerlinger, P., Warten, S., Do morphological anomalies reflect chromosomal aneuploidies? A case report (2000) Hum Reprod, 15, pp. 351-365 Re, J.L., De Méo, M.P., Laget, M., Guiraud, H., Castegnaro, M., Vanelle, P., Evaluation of the genotoxic activity of metronidazole and dimetridazole in human lymphocytes by the comet assay (1997) Mutat Res, 375, pp. 147-155 |
| ISSN: | 08906238 |
| DOI: | 10.1016/j.reprotox.2006.11.010 |