Antiviral effect of a synthetic brassinosteroid on the replication of vesicular stomatitis virus in Vero cells

The antiviral mode of action of the synthetic brassinosteroid (22S,23S)-3β-bromo-5α,22,23-trihydroxystigmastan-6-one (6b) against replication of vesicular stomatitis virus (VSV) in Vero cells was investigated. Time-related experiments showed that 6b mainly affects a late event of the virus growth cy...

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Autor principal: Romanutti, C.
Otros Autores: Castilla, V., Coto, C.E, Wachsman, M.B
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 2007
Acceso en línea:Registro en Scopus
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024 7 |2 scopus  |a 2-s2.0-33847001301 
024 7 |2 cas  |a Antiviral Agents; DNA, Viral; RNA, Viral; Steroids 
040 |a Scopus  |b spa  |c AR-BaUEN  |d AR-BaUEN 
030 |a IAAGE 
100 1 |a Romanutti, C. 
245 1 0 |a Antiviral effect of a synthetic brassinosteroid on the replication of vesicular stomatitis virus in Vero cells 
260 |c 2007 
270 1 0 |m Wachsman, M.B.; Laboratorio de Virología, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Pabellon 2, Piso 4, C1428EGA Buenos Aires, Argentina; email: wachsman@qb.fcen.uba.ar 
506 |2 openaire  |e Política editorial 
504 |a Rose, J.K., Whitt, M.A., Rhabdoviridae: the viruses and their replication (2001) Fundamental virology 4, , Knipe D.M., and Howley P.M. (Eds), Lippincott Williams & Wilkins, Philadelphia, PA 
504 |a (1999) Basic virology, , Wagner E.K., and Hewlett J. (Eds), Blackwell Science Inc., Cambridge, MA 
504 |a Clouse, S.D., Sasse, J.M., Brassinosteroids: essential regulators of plant growth and development. (1998) Annu Rev Plant Physiol Plant Mol Biol, 49, pp. 427-451 
504 |a Fujioka, S., Sakurai, A., Brassinosteroids (1997) Nat Prod Rep, 14, pp. 1-10 
504 |a Wachsman, M.B., López, E.M.F., Ramírez, J.A., Galagovsky, L.R., Coto, C.E., Antiviral effect of brassinosteroids against herpes virus and arenaviruses (2000) Antivir Chem Chemother, 11, pp. 71-77 
504 |a Talarico, L.B., Ramirez, J.A., Galagovsky, L.R., Wachsman, M.B., Structure-activity relationship studies in a set of new brassinosteroid derivatives assayed against herpes simplex virus type 1 and 2 in cell cultures (2002) Med Chem Res, 11, pp. 434-444 
504 |a Wachsman, M.B., Ramírez, J.A., Galagovsky, L.R., Coto, C.E., Antiviral activity of brassinosteroids derivatives against measles virus in cell cultures (2002) Antivir Chem Chemother, 13, pp. 61-66 
504 |a Wachsman, M.B., Castilla, V., Talarico, L.B., Ramirez, J., Galagovsky, L.R., Coto, C.E., Antiherpetic mode of action of (22S,23S)-3β-bromo-5α,22,23-trihidroxiestigmastan-6-one in vitro (2004) Int J Antimicrob Agents, 23, pp. 524-526 
504 |a Castilla, V., Larzabal, M., Sgalippa, N.A., Wachsman, M.B., Coto, C.E., Antiviral mode of action of a synthetic brassinosteroid against Junin virus replication (2005) Antiviral Res, 68, pp. 88-95 
504 |a Wachsman, M.B., Ramírez, J.A., Talarico, L.B., Galagovsky, L.R., Coto, C.E., Antiviral activity of natural and synthetic brassinosteroids (2004) Curr Med Chem Anti Infect Agents, 3, pp. 163-179 
504 |a Teme Centurión, O.M., Galagovsky, L.R., Alternative synthesis of 24(S)-homoethylcastasterone from stigmasterol (1998) Anales de la Asociación Química Argentina, 86, pp. 104-109 
504 |a Denizot, F., Lang, R., Rapid colorimetric assay for cell growth and survival (1986) J Immunol Methods, 89, pp. 271-277 
504 |a Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4 (1970) Nature, 227, pp. 680-685 
504 |a Marcus, P.I., Engelharrdt, D.L., Hunt, J.M., Sekellick, M.J., Interferon action: inhibition of vesicular stomatitis virus RNA synthesis induced by virion-bound polymerase (1971) Science, 174, pp. 593-598 
504 |a Dargan, D.J., Galt, C.B., Subak-Sharpe, J.H., The effect of cicloxolone sodium on the replication of vesicular stomatitis virus in BSC-1 cells (1992) J Gen Virol, 73, pp. 397-406 
504 |a Wehling, M., Specific nongenomic actions of steroid hormones (1997) Annu Rev Physiol, 59, pp. 365-393 
504 |a Falkenstein, E., Tillman, H.C., Christ, M., Feuring, M., Wehling, M., Multiple actions of steroid hormones-a focus on rapid, nongenomic effects (2000) Pharmacol Rev, 52, pp. 513-555 
504 |a Liu, D., Dillon, J.S., Dehydroepiandrosterone activates endothelial cell nitric-oxide synthase by a specific plasma membrane receptor coupled to Gα(i2,3) (2002) J Biol Chem, 277, pp. 21379-21388 
504 |a Mussig, C., Lisso, J., Coll-Garcia, D., Altman, T., Molecular analysis of brassinosteroid action (2006) Plant Biol, 9, pp. 291-296 
504 |a Sharma, A., Matsuoka, M., Tanaka, H., Komatsu, S., Antisense inhibition of a BRI1 receptor reveals additional protein kinase signaling components downstream to the perception of brassinosteroid in rice (2001) FEBS Lett, 507, pp. 346-350 
504 |a Khripach, V., Zhabinskii, V., De Groot, A., Twenty years of brassinosteroids: steroidal plant hormones warrant better crops for the XXI century (2000) Ann Bot (Lond), 86, pp. 441-447 
504 |a Michelini, F.M., Ramírez, J.A., Berra, A., Galagovsky, L.R., Alché, L.E., In vitro and in vivo antiherpetic activity of three new synthetic brassinosteroid analogues (2004) Steroids, 69, pp. 713-720 
520 3 |a The antiviral mode of action of the synthetic brassinosteroid (22S,23S)-3β-bromo-5α,22,23-trihydroxystigmastan-6-one (6b) against replication of vesicular stomatitis virus (VSV) in Vero cells was investigated. Time-related experiments showed that 6b mainly affects a late event of the virus growth cycle. Virus adsorption, internalisation and early RNA synthesis are not the target of the inhibitory action. Results obtained indicate that the antiviral compound adversely affects virus protein synthesis and viral mature particle formation. © 2006 Elsevier B.V. and the International Society of Chemotherapy.  |l eng 
536 |a Detalles de la financiación: Universidad de Buenos Aires 
536 |a Detalles de la financiación: Umweltbundesamt, X 046 
536 |a Detalles de la financiación: National Research Council 
536 |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas, PIP 02236 
536 |a Detalles de la financiación: We thank Dr L.R. Galagovsky and J.A. Ramírez for providing the compound 6b. This work was supported by grants from the University of Buenos Aires, UBA, X 046, and the National Research Council, CONICET, PIP 02236. Dr Celia E. Coto is a member of the Scientific Career of CONICET, Argentina. 
593 |a Laboratorio de Virología, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, C1428EGA Buenos Aires, Argentina 
690 1 0 |a (22S,23S)-3Β-BROMO-5Α,22,23-TRIHYDROXYSTIGMASTAN-6-ONE 
690 1 0 |a ANTIVIRAL ACTIVITY 
690 1 0 |a BRASSINOSTEROIDS 
690 1 0 |a VESICULAR STOMATITIS VIRUS 
690 1 0 |a 3BETA BROMO 5ALPHA,22,23 TRIHYDROXYSTIGMASTAN 6 ONE 
690 1 0 |a BRASSINOSTEROID 
690 1 0 |a STEROID HORMONE 
690 1 0 |a UNCLASSIFIED DRUG 
690 1 0 |a ANIMAL CELL 
690 1 0 |a ANTIVIRAL ACTIVITY 
690 1 0 |a ARTICLE 
690 1 0 |a CONTROLLED STUDY 
690 1 0 |a DOSE TIME EFFECT RELATION 
690 1 0 |a INTERNALIZATION 
690 1 0 |a NONHUMAN 
690 1 0 |a PRIORITY JOURNAL 
690 1 0 |a PROTEIN SYNTHESIS 
690 1 0 |a RNA SYNTHESIS 
690 1 0 |a STOMATITIS 
690 1 0 |a VERO CELL 
690 1 0 |a VESICULAR STOMATITIS VIRUS 
690 1 0 |a VIROGENESIS 
690 1 0 |a VIRUS ADSORPTION 
690 1 0 |a VIRUS INHIBITION 
690 1 0 |a VIRUS REPLICATION 
690 1 0 |a ANIMALS 
690 1 0 |a ANTIVIRAL AGENTS 
690 1 0 |a BASE SEQUENCE 
690 1 0 |a CERCOPITHECUS AETHIOPS 
690 1 0 |a DNA, VIRAL 
690 1 0 |a MOLECULAR STRUCTURE 
690 1 0 |a RNA, VIRAL 
690 1 0 |a STEROIDS 
690 1 0 |a VERO CELLS 
690 1 0 |a VESICULAR STOMATITIS-INDIANA VIRUS 
690 1 0 |a VIRUS REPLICATION 
700 1 |a Castilla, V. 
700 1 |a Coto, C.E. 
700 1 |a Wachsman, M.B. 
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