Saccharomyces cerevisiae porphobilinogenase: Some physical and kinetic properties
1. 1. Properties of porphobilinogenase (PBGase), the enzyme complex converting porphobilinogen (PBG) into uroporphyrinogens, were studied in a wild strain, D273-10B and a mutant, B231, of Saccharomyces cerevisiae 2. 2. A well-defined maximum of enzyme activity was observed for the mutant strain afte...
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1989
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| LEADER | 10177caa a22009737a 4500 | ||
|---|---|---|---|
| 001 | PAPER-660 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203000.0 | ||
| 008 | 190411s1989 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0024489040 | |
| 024 | 7 | |2 cas |a magnesium, 7439-95-4; porphobilinogenase, 9055-40-7; sodium, 7440-23-5; Ammonia-Lyases, EC 4.3.1.; Magnesium, 7439-95-4; porphobilinogenase, EC 5.-; Sodium, 7440-23-5 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 100 | 1 | |a Araujo, L.S. | |
| 245 | 1 | 0 | |a Saccharomyces cerevisiae porphobilinogenase: Some physical and kinetic properties |
| 260 | |c 1989 | ||
| 270 | 1 | 0 | |m Batlle, A.M.d.C.; Centro de Investigaciones sobre Porfirinas y Porfirias, CIPYP (CONICET-FCEN, UBA), Ciudad Universitaria, Pabellon II, 2do. Piso, 1428 Buenos Aires, Argentina |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Araujo, Lombardo, Rossetti, Batlle, Porphobilinogenase activity in a wild strain and its haem mutant of Saccharomyces cerevisiae (1987) Revta argent., 19, pp. 109-120 | ||
| 504 | |a Arrese, Carvajal, Robison, Sambunaris, Panek, Mattoon, Cloning of the δ-aminolevulinic acid synthase structural gene in yeast (1983) Curr. Genet., 7, pp. 175-183 | ||
| 504 | |a Batlle, Llambias, Wider de Xifra, Tigier, Porphyrin biosynthesis in the soybean callus tissue system—XV. The effect of growth conditions (1975) Int. J. Biochem., 6, pp. 591-606 | ||
| 504 | |a Bilinski, Litwinska, Lukaszkiewicz, Rytka, Simon, Labbe-Bois, Characterization of two mutant strains of Saccharomyces cerevisiae deficient in coproporphyrinogen 3-oxidase activity (1981) J. gen. Microbiol., 122, pp. 79-87 | ||
| 504 | |a Clement, Kohashi, Piper, Rat liver purification uroporphyrinogen III cosynthetase (1982) Archs Biochem. Biophys., 214, pp. 657-663 | ||
| 504 | |a Cornford, Transformation of porphobilinogen into porphyrins by preparations from human erythrocytes (1964) Biochem. J., 91, pp. 64-73 | ||
| 504 | |a Fumagalli, Rossetti, Juknat de Geralnik, Kotler, Batlle, Estudios sobre la PBGasa de hígado de cerdo (1982) An. Asoc. quím. argent., 70, pp. 375-382 | ||
| 504 | |a Juknat, Biosintesis de porfirinógenos (1983) Ph.D. Thesis, , University of Buenos Aires | ||
| 504 | |a Juknat, Kotler, Koopmann, Batlle, Porphobilinogenase from Rhodopseudomonas palustris (1989) Comp. Biochem. Physiol., 92 B, pp. 291-295 | ||
| 504 | |a Labbe-Bois, Urban-Grimal, Volland, Camadro, Dehoux, About the regulation of protoheme synthesis in the yeast Saccharomyces cerevisiae (1983) Mitochondria, 523, p. 534 | ||
| 504 | |a Llambías, Batlle, Porphyrin biosynthesis in soybean-callus—V. The porphobilinogen deaminase-uroporphyrinogen cosynthetase system (1970) Kinetic studies, 220, pp. 552-559. , Biochim. biophys. Acta | ||
| 504 | |a Llambías, Batlle, Studies on the porphobilinogen deaminase-uroporphyrinogen cosynthetase system of cultured soya-bean cells. (1971) Biochem J, 121, pp. 327-340 | ||
| 504 | |a Llambías, Batlle, Porphyrin biosynthesis—VIII. Avian erythrocyte porphibilinogen deaminase-uroporphyrinogen III cosynthetase (1971) Its purification, properties and the separation of its components, 227, pp. 180-191. , Biochim. biophys. Acta | ||
| 504 | |a Lowry, Rosebrough, Farr, Randall, Protein measurement with the Folin phenol reagent (1951) J. biol. Chem., 193, pp. 265-275 | ||
| 504 | |a Mattoon, Malamud, Brunner, Braz, Carvajal, Lancanshire, Panek, Regulation of heme formation and cytochrome biosynthesis in normal and mutant yeast (1978) Biochemistry and Genetics of Yeast, Pure and Applied Aspects, pp. 317-337. , M. Bacila, B. Horecker, A.O.M. Stoppani, Academic Press, New York | ||
| 504 | |a Moore, Labbe, Assays for ALA and PBG determination (1964) Clin. Chem., 10, pp. 1105-1109 | ||
| 504 | |a Rimington, Spectral-absorption coefficients of some porphyrins in the Soret-band region. (1960) Biochem J, 75, pp. 620-623 | ||
| 504 | |a Rossetti, Batlle, Polypirrol intermediates in porphyrin biosynthesis (1977) Studies with Euglena gracilis, 8, pp. 277-283. , Int. J. Biochem | ||
| 504 | |a Rossetti, Lombardo, Juknat de Geralnik, Araujo, Batlle, Porphyrin biosynthesis in Euglena gracilis—V. Soluble and particulate PBG-ase (1986) Comp. Biochem. Physiol., 85 B, pp. 451-458 | ||
| 504 | |a Rossetti, Araujo, Lombardo, Correa Garcia, Batlle, Porphyrin biosynthesis in Euglena gracilis—VI. The effect of growth conditions on porphobilinogenase activity and further properties (1987) Comp. Biochem. Physiol., 87 B, pp. 593-600 | ||
| 504 | |a Rykta, Bilinski, Labbe-Bois, Modified uroporphyrinogen decarboxylase activity in yeast mutant which mimics porphyria cutanea tarda (1984) Biochem J., 218, pp. 405-413 | ||
| 504 | |a Sancovich, Batlle, Grinstein, Porphyrin biosynthesis—VI. Separation and purification of porphobilinogen deaminase and uroporphyrinogen isomerase from cow liver (1969) Porphobilinogenase as an allosteric enzyme, 191, pp. 130-143. , Biochim. biophys. Acta | ||
| 504 | |a Sancovich, Ferramola, Batlle, Grinstein, Preparation of porphobilinogen (1970) Methods in Enzymology, 17 A, pp. 220-222. , H. Tabor, C.W. Tabor, Academic Press, New York | ||
| 504 | |a Sancovich, Ferramola, Batlle, Kivilevich, Grinstein, Studies on cow liver porphobilinogen deaminase (1976) Acta physiol. latinoam., 26, pp. 376-386 | ||
| 504 | |a Sanders, Mied, Briquet, Hernández-Rodríguez, Gottal, Mattoon, Regulation of mitochondrial biogenesis: yeast mutants deficient in synthesis of δ-aminolevulinic acid (1973) J. molec. Biol., 80, pp. 17-39 | ||
| 504 | |a Urban-Grimal, Labbe-Bois, Genetic and biochemical characterization of mutants of Saccharomyces cerevisiae blocked in six different steps of heme biosynthesis (1981) Molec. Gen. Genet., 183, pp. 85-92 | ||
| 504 | |a Wider de Xifra, Batlle, Tigier, δ-aminolaevulinate synthetase in extracts of cultured soybean cells (1971) Biochimica et Biophysica Acta (BBA) - Enzymology, 235, pp. 511-517 | ||
| 520 | 3 | |a 1. 1. Properties of porphobilinogenase (PBGase), the enzyme complex converting porphobilinogen (PBG) into uroporphyrinogens, were studied in a wild strain, D273-10B and a mutant, B231, of Saccharomyces cerevisiae 2. 2. A well-defined maximum of enzyme activity was observed for the mutant strain after 20 hr of growth; whilst the activity in the wild strain did not vary significantly during growth. 3. 3. Neither PBG consumption nor uroporphyrinogen formation were modified by the presence of air either in the wild or in the mutant strain. 4. 4. In both the wild and mutant strains uroporphyrinogen formation increased linearly with both protein concentration and incubation time. 5. 5. The addition of a mixture of sodium and magnesium salts to the assay system inhibited the enzyme activity of both strains by 50% without modifying the isomer composition. 6. 6. The same optimum pH (7.4) and mol. wt (50,000 ± 5000) was found for the enzyme from both strains. 7. 7. The enzyme from both the wild and mutant strains shows Michaelis-Menten kinetics when isolated from cells at either the exponential or the stationary phases of growth. Accumulation of porphyrins and δ-aminolevulinic acid occurring during the exponential phase in the mutant strain, did not modify the kinetics. © 1989. |l eng | |
| 536 | |a Detalles de la financiación: National Council for Scientific Research | ||
| 536 | |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas | ||
| 536 | |a Detalles de la financiación: Acknowledgements--M. V. Rossetti and A. M. del C. Batlle hold posts as Scientific Researchers in the Argentine National Research Council (CONICET). M. E. Lombardo is a Research Fellow from the University of Buenos Aires. This work was supported by grants from the CONICET, the SUBCYT, Secretaria de Salud POblica del Ministerio de Bienestar Social, Banco de la Naci6n Argentina and Banco de Galicia y Buenos Aires. This paper forms part of the Doctoral Thesis of L. S. Araujo to be presented to the University of Buenos Aires for her Ph.D. Degree. We wish to express our heartfelt thanks to Professor J. Mattoon, from the Department of Biochemistry, University of Colorado, USA, for generously providing us with all the strains of S. cerevisiae and above all for his continued interest and encouragement. The technical assistance of Mrs B. Riccillo de Aprea is gratefully acknowledged. | ||
| 593 | |a Centro de Investigaciones sobre Porfirinas y Porfirias, CIPYP (CONICET-FCEN, UBA), Ciudad Universitaria, Pabellon II, 2do. Piso, 1428 Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a AMMONIA LYASE |
| 690 | 1 | 0 | |a AMMONIA LYASES |
| 690 | 1 | 0 | |a MAGNESIUM |
| 690 | 1 | 0 | |a PORPHOBILINOGENASE |
| 690 | 1 | 0 | |a SODIUM |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a ENZYMOLOGY |
| 690 | 1 | 0 | |a GENETICS |
| 690 | 1 | 0 | |a KINETICS |
| 690 | 1 | 0 | |a METABOLISM |
| 690 | 1 | 0 | |a MOLECULAR WEIGHT |
| 690 | 1 | 0 | |a MUTATION |
| 690 | 1 | 0 | |a SACCHAROMYCES CEREVISIAE |
| 690 | 1 | 0 | |a AMMONIA-LYASES |
| 690 | 1 | 0 | |a HYDROGEN-ION CONCENTRATION |
| 690 | 1 | 0 | |a KINETICS |
| 690 | 1 | 0 | |a MAGNESIUM |
| 690 | 1 | 0 | |a MOLECULAR WEIGHT |
| 690 | 1 | 0 | |a MUTATION |
| 690 | 1 | 0 | |a SACCHAROMYCES CEREVISIAE |
| 690 | 1 | 0 | |a SODIUM |
| 690 | 1 | 0 | |a SUPPORT, NON-U.S. GOV'T |
| 650 | 1 | 7 | |2 spines |a PH |
| 700 | 1 | |a Lombardo, M.E. | |
| 700 | 1 | |a Rossetti, ]M.V. | |
| 700 | 1 | |a Batlle, A.M.d.C. | |
| 773 | 0 | |d 1989 |g v. 92 |h pp. 297-301 |k n. 2 |x 03050491 |w (AR-BaUEN)CENRE-2752 |t Comparative Biochemistry and Physiology -- Part B: Biochemistry and | |
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