Biosynthesis of digitoxin in Digitalis purpurea

Simultaneous administration of a mixture of [21-3H]-3β, 20ξ-dihydroxy-23-nor-5β-cholanoic acid and [21-14C]-3β-hydroxy-5β-pregnan-20-one to Digitalis purpurea intact plants produced double labelled digitoxin. The 3H: 14C ratio of the cardenolide was considerably higher than the 3H: 14C ratio of the...

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Autor principal: Deluca, M.E
Otros Autores: Seldes, A.M, Gros, E.G
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 1989
Acceso en línea:Registro en Scopus
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Registro en la Biblioteca Digital
Aporte de:Registro referencial: Solicitar el recurso aquí
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Sumario:Simultaneous administration of a mixture of [21-3H]-3β, 20ξ-dihydroxy-23-nor-5β-cholanoic acid and [21-14C]-3β-hydroxy-5β-pregnan-20-one to Digitalis purpurea intact plants produced double labelled digitoxin. The 3H: 14C ratio of the cardenolide was considerably higher than the 3H: 14C ratio of the administered substrates. This result indicates that in the main biosynthetic pathway from a 20-keto-pregnane to a cardenolide the introduction of an acetate unit at C-20 should occur before the hydroxylation at C-21. © 1989.
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ISSN:00319422
DOI:10.1016/0031-9422(89)85019-8