Proteomic analysis identified N-cadherin, clusterin, and HSP27 as mediators of SPARC (secreted protein, acidic and rich in cysteines) activity in melanoma cells
Secreted protein, acidic and rich in cysteines (SPARC) is a secreted protein associated with increased aggressiveness of different human cancer types. In order to identify downstream mediators of SPARC activity, we performed a 2-DE proteomic analysis of human melanoma cells following antisense-media...
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2007
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
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| 008 | 190411s2007 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-36549055112 | |
| 024 | 7 | |2 cas |a cysteine, 4371-52-2, 52-89-1, 52-90-4; osteonectin, 104052-78-0; Cadherins; Clusterin; HSPB1 protein, human; Heat-Shock Proteins; Neoplasm Proteins; Osteonectin | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a PROTC | ||
| 100 | 1 | |a Sosa, M.S. | |
| 245 | 1 | 0 | |a Proteomic analysis identified N-cadherin, clusterin, and HSP27 as mediators of SPARC (secreted protein, acidic and rich in cysteines) activity in melanoma cells |
| 260 | |c 2007 | ||
| 270 | 1 | 0 | |m Llera, A.S.; Fundación Instituto Leloir, Av. Patricias Argentinas 435, 1405BWE Buenos Aires, Argentina; email: allera@leloir.org.ar |
| 506 | |2 openaire |e Política editorial | ||
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| 504 | |a Prada, F., Benedetti, L.G., Bravo, A.I., Alvarez, M.J., SPARC endogenous level, rather than fibroblast-produced SPARC or stroma reorganization induced by SPARC, is responsible for melanoma cell growth (2007) J. Invest. Dermatol, , in press, DOI:10.1038/sj.jid.5700962 | ||
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| 520 | 3 | |a Secreted protein, acidic and rich in cysteines (SPARC) is a secreted protein associated with increased aggressiveness of different human cancer types. In order to identify downstream mediators of SPARC activity, we performed a 2-DE proteomic analysis of human melanoma cells following antisense-mediated downregulation of SPARC expression. We found 23/504 differential spots, 15 of which were identified by peptide fingerprinting analysis. Three of the differential proteins (N-cadherin (N-CAD), clusterin (CLU), and HSP27) were validated by immunoblotting, confirming decreased levels of N-CAD and CLU and increased amounts of HSP27 in conditioned media of cells with diminished SPARC expression. Furthermore, transient knock down of SPARC expression in melanoma cells following adenoviral-mediated transfer of antisense RNA confirmed these changes. We next developed two different RNAs against SPARC that were able to inhibit in vivo melanoma cell growth. Immunoblotting of the secreted fraction of RNAi-transfected melanoma cells confirmed that downregulation of SPARC expression promoted decreased levels of N-CAD and CLU and increased secretion of HSP27. Transient re-expression of SPARC in SPARC-downregulated cells reverted extracellular N-CAD, CLU, and HSP27 to levels similar to those in the control. These results constitute the first evidence that SPARC, N-CAD, CLU, and HSP27 converge in a unique molecular network in melanoma cells. © 2007 WILEY-VCH Verlag GmbH & Co. KGaA. |l eng | |
| 593 | |a Laboratory of Molecular and Cellular Therapy, Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina | ||
| 593 | |a Proteomics Unit, Centro Nacional de Biotecnología (CNB), Madrid, Spain | ||
| 593 | |a Fundación Instituto Leloir, Av. Patricias Argentinas 435, 1405BWE Buenos Aires, Argentina | ||
| 593 | |a Department of Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, PA, United States | ||
| 593 | |a Proteomics Unit, Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain | ||
| 690 | 1 | 0 | |a MELANOMA |
| 690 | 1 | 0 | |a OSTEONECTIN |
| 690 | 1 | 0 | |a SECRETED PROTEIN |
| 690 | 1 | 0 | |a SPARC |
| 690 | 1 | 0 | |a TWO-DIMENSIONAL GEL ELECTROPHORESIS |
| 690 | 1 | 0 | |a CLUSTERIN |
| 690 | 1 | 0 | |a COMPLEMENTARY RNA |
| 690 | 1 | 0 | |a CYSTEINE |
| 690 | 1 | 0 | |a HEAT SHOCK PROTEIN 27 |
| 690 | 1 | 0 | |a NERVE CELL ADHESION MOLECULE |
| 690 | 1 | 0 | |a OSTEONECTIN |
| 690 | 1 | 0 | |a PROTEIN SPARC |
| 690 | 1 | 0 | |a UNCLASSIFIED DRUG |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a CELL GROWTH |
| 690 | 1 | 0 | |a CONTROLLED STUDY |
| 690 | 1 | 0 | |a DOWN REGULATION |
| 690 | 1 | 0 | |a HUMAN |
| 690 | 1 | 0 | |a HUMAN CELL |
| 690 | 1 | 0 | |a IMMUNOBLOTTING |
| 690 | 1 | 0 | |a MELANOMA |
| 690 | 1 | 0 | |a MELANOMA CELL |
| 690 | 1 | 0 | |a PRIORITY JOURNAL |
| 690 | 1 | 0 | |a PROTEIN ANALYSIS |
| 690 | 1 | 0 | |a PROTEIN EXPRESSION |
| 690 | 1 | 0 | |a PROTEIN FUNCTION |
| 690 | 1 | 0 | |a PROTEOMICS |
| 690 | 1 | 0 | |a CADHERINS |
| 690 | 1 | 0 | |a CELL LINE, TUMOR |
| 690 | 1 | 0 | |a CLUSTERIN |
| 690 | 1 | 0 | |a HEAT-SHOCK PROTEINS |
| 690 | 1 | 0 | |a HUMANS |
| 690 | 1 | 0 | |a MELANOMA |
| 690 | 1 | 0 | |a NEOPLASM PROTEINS |
| 690 | 1 | 0 | |a OSTEONECTIN |
| 690 | 1 | 0 | |a PROTEOMICS |
| 690 | 1 | 0 | |a TUMOR CELLS, CULTURED |
| 700 | 1 | |a Girotti, M.R. | |
| 700 | 1 | |a Salvatierra, E. | |
| 700 | 1 | |a Prada, F. | |
| 700 | 1 | |a De Olmo, J.A.L. | |
| 700 | 1 | |a Gallango, S.J. | |
| 700 | 1 | |a Albar, J.P. | |
| 700 | 1 | |a Podhajcer, O.L. | |
| 700 | 1 | |a Llera, A.S. | |
| 773 | 0 | |d 2007 |g v. 7 |h pp. 4123-4134 |k n. 22 |p Proteomics |x 16159853 |t Proteomics | |
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