Stabilization studies of Fomes sclerodermeus laccases

Stability of laccase isoenzymes from a crude extract obtained from Fomes sclerodermeus grown on wheat bran medium was studied. The variables assessed were temperature, pH and additives. As revealed by PAGE, three bands of laccase, each with different thermal inactivation pattern, were detected in th...

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Autor principal: Papinutti, L.
Otros Autores: Dimitriu, P., Forchiassin, F.
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 2008
Materias:
PH
Acceso en línea:Registro en Scopus
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Registro en la Biblioteca Digital
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024 7 |2 scopus  |a 2-s2.0-35548971158 
024 7 |2 cas  |a 1 hydroxybenzotriazole, 2592-95-2; copper sulfate, 7758-98-7, 7758-99-8; glutaraldehyde, 111-30-8, 37245-61-7; glycerol, 56-81-5; laccase, 80498-15-3; mannitol, 69-65-8, 87-78-5; trehalose, 99-20-7; veratryl alcohol, 93-03-8; Isoenzymes; Laccase, 1.10.3.2 
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030 |a BIRTE 
100 1 |a Papinutti, L. 
245 1 0 |a Stabilization studies of Fomes sclerodermeus laccases 
260 |c 2008 
270 1 0 |m Papinutti, L.; Laboratorio de Micología Experimental, Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, 1428 Buenos Aires, Argentina; email: leandru@bg.fcen.uba.ar 
506 |2 openaire  |e Política editorial 
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504 |a Aymard, C., Belarbi, A., Kinetics of thermal deactivation of enzymes: a simple three parameters phenomenological model can describe the decay of enzyme activity, irrespectively of the mechanism (2000) Enzyme Microb. Technol., 27, pp. 612-618 
504 |a Baptista, R.P., Cabral, J.M.S., Melo, E.P., Trehalose delays the reversible but not the irreversible thermal denaturation of cutinase (2000) Biotechnol. Bioeng., 70, pp. 699-703 
504 |a Bourbonnais, R., Paice, M.G., Freiermuth, B., Bodie, E., Borneman, S., Reactivities of various mediators and laccases with kraft pulp and lignin model compounds (1997) Appl. Environ. Microbiol., 63, pp. 4627-4632 
504 |a Bourbonnais, R., Paice, M.G., Reid, I.D., Lanthier, P., Yaguchi, M., Lignin oxidation by laccase isozymes from Trametes versicolor and role of the mediator 2,2′-azino-bis(ethylbenzothiazoline-6-sulfonic acid) in Kraft lignin depolimerization (1995) Appl. Environ. Microbiol., 61, pp. 1876-1880 
504 |a Chefetz, B., Chen, Y., Hadar, Y., Purification and characterization of laccase from Chaetomium thermophilium and its role in humification (1998) Appl. Environ. Microbiol., 64, pp. 3175-3179 
504 |a Collins, P.J., Dobson, A.D.W., Field, J.A., Reduction of the 2,2′-azinobis(3ethylbenzthiazoline 6-sulfonate) cation radical by physiological organic acids in the absence and presence of manganese (1998) Appl. Environ. Microbiol., 64, pp. 2026-2031 
504 |a Costa, S.A., Tzanov, T., Carneiro, A.F., Para, A., Gübitz, G.M., Cavaco-Paulo, A., Studies of stabilization of native catalase using additives (2002) Enzyme Microb. Technol., 30, pp. 387-391 
504 |a Fenice, M., Giovannozzi, S.G., Federici, F., D'Annibale, A., Submerged and solid-state production of laccase and Mn-peroxidase by Panus tigrinus on olive mill wastewater-based media (2003) J. Biotechnol., 100, pp. 77-85 
504 |a Galhaup, C., Wagner, H., Hinterstoisser, B., Haltrich, D., Increased production of laccase by the wood-degrading basidiomycete Trametes pubescens (2002) Enzyme Microb. Technol., 30, pp. 529-536 
504 |a Gómez, J., Pazos, M., Rodríguez-Couto, S., Sanromán, M.A., Chestnut shell and barley bran as potential substrates for laccase production by Coriolopsis rigida under solid-state conditions (2005) J. Food Eng., 68, pp. 315-319 
504 |a Kirk, T.K., Farrell, R.L., Enzymatic "combustion": the microbial degradation of lignin (1987) Annu. Rev. Microbiol., 41, pp. 465-505 
504 |a Koroleva, O.V., Gravilova, V.P., Stepanova, E.V., Lebedeva, V.I., Sverdlova, N.I., Landesman, E.O., Yavmetdinov, I.S., Yaropolov, A.I., Production of lignin modifying enzymes by co-cultivated white-rot fungi Cerrena maxima and Coriolus hirsutus and characterization of laccase from Cerrena maxima (2002) Enzyme Microb. Technol., 30, pp. 573-580 
504 |a Koroleva, O.V., Stepanova, E.V., Binukov, V.I., Timofeev, V.P., Pfeil, W., Temperature-induced changes in copper centers and protein conformation of two fungal laccases from Coriolus hirsutus and Coriolus zonatus (2001) Biochim. Biophys. Acta, 1547, pp. 397-407 
504 |a Lo, S.C., Ho, Y.S., Buswell, J.A., Effect of phenolic monomers on the production of laccases by the edible mushroom Pleurotus sajor-caju, and partial characterization of a major laccase component (2001) Mycologia, 93, pp. 413-421 
504 |a Lozano, P., Combes, D., Iborra, J.L., Effects of polyols on α-chymotrypsin thermostability a mechanistic analysis of the enzyme stabilization (1994) J. Biotechnol., 35, pp. 9-18 
504 |a Muñoz, C., Guillén, F., Martínez, A.T., Martínez, M.J., Induction and characterization of laccase in the ligninolytic fungus Pleurotus eryngii (1997) Curr. Microbiol., 34, pp. 1-5 
504 |a Palmieri, G., Giardina, P., Bianco, C., Fontanella, B., Sannia, G., Copper induction of laccase isoenzymes in the ligninolytic fungus Pleurotus ostreatus (2000) Appl. Environ. Microbiol., 66, pp. 920-924 
504 |a Papinutti, V.L., Diorio, L.A., Forchiassin, F., Production of laccase and manganese peroxidase by Fomes sclerodermeus grown on wheat bran (2003) J. Ind. Microbiol. Biotechnol., 30, pp. 157-160 
504 |a Papinutti, V.L., Forchiassin, F., Modification of malachite green by Fomes sclerodermeus and reduction of toxicity to Phanerochaete chrysosporium (2004) FEMS Microbiol. Lett., 231, pp. 205-209 
504 |a Pointing, S.B., Feasibility of bioremediation by white-rot fungi (2001) Appl. Microbiol. Biotechnol., 57, pp. 20-33 
504 |a Pointing, S.B., Jones, E.B.G., Vrijmoed, L.L.P., Optimization of laccase production by Pycnoporus sanguineus in submerged liquid cultures (2000) Mycologia, 92, pp. 139-144 
504 |a Renate, U.H., Ulrich, A.J.M., The concept of the unfolding region for approaching the mechanisms of enzyme stabilization (1999) J. Mol. Cat. B Enz., 7, pp. 125-131 
504 |a Thurston, C.F., The structure and function of fungal laccases (1994) Microbiology-UK, 140, pp. 19-26 
504 |a Ullrich, R., Huong, L.M., Dung, N.L., Laccase from the medicinal mushroom Agaricus blazei: production, purification and characterization (2005) Appl. Microbiol. Biotechnol., 67, pp. 357-363 
504 |a Viniegra-Gonzáles, G., Favela-Torres, E., Aguilar, C.N., Rómero-Gómez, S.J., Díaz-Godínez, G., Augur, C., Advantages of fungal enzyme production in solid state over liquid fermentation systems (2003) Biochem. Eng. J., 13, pp. 157-167 
520 3 |a Stability of laccase isoenzymes from a crude extract obtained from Fomes sclerodermeus grown on wheat bran medium was studied. The variables assessed were temperature, pH and additives. As revealed by PAGE, three bands of laccase, each with different thermal inactivation pattern, were detected in the crude extract: after 6 h at 50 °C and pH 8, Lc2 was the most resistant, while the Lc1 and Lc3 bands were almost completely inactivated. This pattern of inactivation was observed at all temperatures and pH tested. Laccase activity was more stable in the 5-10 pH range when incubated at 40 and 50 °C; at 30 °C and 24 h the enzyme remained fully active in the 3-11 pH range. The effect of additives (veratryl alcohol, trehalose, glycerol, mannitol, glutaraldehyde, CuSO4 and 1-HBT) on laccase stability was tested. The stability was enhanced with CuSO4 (1.25 mM), glycerol (0.2%) and mannitol (1%). The presence of both CuSO4 and glycerol caused a 3-fold increase in the half-life values. © 2007 Elsevier Ltd. All rights reserved.  |l eng 
536 |a Detalles de la financiación: Universidad de Buenos Aires 
536 |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas 
536 |a Detalles de la financiación: This work was supported by CONICET (Consejo Nacional de Investigaciones Cientı´ficas y Técnicas) Argentina and Universidad de Buenos Aires. 
593 |a Laboratorio de Micología Experimental, Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, 1428 Buenos Aires, Argentina 
690 1 0 |a ADDITIVES 
690 1 0 |a FOMES SCLERODERMEUS 
690 1 0 |a LACCASE 
690 1 0 |a STABILITY 
690 1 0 |a FOMES SCLERODERMEUS 
690 1 0 |a INACTIVATION PATTERN 
690 1 0 |a LACCASE 
690 1 0 |a ADDITIVES 
690 1 0 |a ALDEHYDES 
690 1 0 |a GLYCEROL 
690 1 0 |a PH EFFECTS 
690 1 0 |a THERMAL EFFECTS 
690 1 0 |a ENZYMES 
690 1 0 |a 1 HYDROXYBENZOTRIAZOLE 
690 1 0 |a COPPER SULFATE 
690 1 0 |a DRUG ADDITIVE 
690 1 0 |a GLUTARALDEHYDE 
690 1 0 |a GLYCEROL 
690 1 0 |a LACCASE 
690 1 0 |a MANNITOL 
690 1 0 |a TREHALOSE 
690 1 0 |a UNCLASSIFIED DRUG 
690 1 0 |a VERATRYL ALCOHOL 
690 1 0 |a ADDITIVES 
690 1 0 |a ALDEHYDES 
690 1 0 |a ENZYMES 
690 1 0 |a GLYCEROL 
690 1 0 |a PH EFFECTS 
690 1 0 |a THERMAL EFFECTS 
690 1 0 |a ELECTROKINESIS 
690 1 0 |a ENZYME ACTIVITY 
690 1 0 |a TEMPERATURE EFFECT 
690 1 0 |a WHEAT 
690 1 0 |a ARTICLE 
690 1 0 |a BASIDIOMYCETES 
690 1 0 |a ENZYME STABILITY 
690 1 0 |a FOMES SCLERODERMEUS 
690 1 0 |a HALF LIFE TIME 
690 1 0 |a NONHUMAN 
690 1 0 |a PRIORITY JOURNAL 
690 1 0 |a TEMPERATURE 
690 1 0 |a WHEAT BRAN 
690 1 0 |a BASIDIOMYCOTA 
690 1 0 |a ENZYME STABILITY 
690 1 0 |a HYDROGEN-ION CONCENTRATION 
690 1 0 |a ISOENZYMES 
690 1 0 |a KINETICS 
690 1 0 |a LACCASE 
690 1 0 |a THERMODYNAMICS 
690 1 0 |a TRITICUM 
690 1 0 |a FOMES 
690 1 0 |a TRITICUM AESTIVUM 
650 1 7 |2 spines  |a PH 
650 1 7 |2 spines  |a PH 
700 1 |a Dimitriu, P. 
700 1 |a Forchiassin, F. 
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