Regulation of ovarian angiogenesis and apoptosis by GnRH-I analogs
An adequate vascular supply is important to provide endocrine and paracrine signals during follicular development. We evaluated the direct in vivo effects of both the GnRH-agonist Leuprolide acetate (LA) and the GnRH-antagonist Antide (Ant) on the expression of VEGF-A and ANPT-1 and their receptors...
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| Otros Autores: | , , |
| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
2008
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
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| LEADER | 20443caa a22016457a 4500 | ||
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| 001 | PAPER-5996 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203540.0 | ||
| 008 | 190411s2008 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-40049085911 | |
| 024 | 7 | |2 cas |a caspase 3, 169592-56-7; iturelix, 112568-12-4; leuprorelin, 53714-56-0, 74381-53-6; vasculotropin A, 489395-96-2; Angiopoietin-1; Caspase 3, EC 3.4.22.-; Gonadotropin-Releasing Hormone, 33515-09-2; iturelix, 112568-12-4; Leuprolide, 53714-56-0; Oligopeptides; Receptor, TIE-2, EC 2.7.1.112; Vascular Endothelial Growth Factor A; vascular endothelial growth factor A, rat; Vascular Endothelial Growth Factor Receptor-2, EC 2.7.1.112 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a MREDE | ||
| 100 | 1 | |a Parborell, F. | |
| 245 | 1 | 0 | |a Regulation of ovarian angiogenesis and apoptosis by GnRH-I analogs |
| 260 | |c 2008 | ||
| 270 | 1 | 0 | |m Tesone, M.; Institute de Biologia Y Medicina Experimental, Obligado 2490, 1428 Buenos Aires, Argentina; email: mtesone@dna.uba.ar |
| 506 | |2 openaire |e Política editorial | ||
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| 520 | 3 | |a An adequate vascular supply is important to provide endocrine and paracrine signals during follicular development. We evaluated the direct in vivo effects of both the GnRH-agonist Leuprolide acetate (LA) and the GnRH-antagonist Antide (Ant) on the expression of VEGF-A and ANPT-1 and their receptors in ovarian follicles from prepubertal eCG-treated rats. We also examined whether the changes observed in apoptosis by GnRH-I analogs have an effect on the caspase cascade. LA significantly decreased the levels of VEGF-A, its receptor Flk-1, and ANPT-1 when compared to controls, while the co-injection of Ant interfered with this effect. No changes were observed in the levels of Tie-2 after treatment with these analogs. When we measured the follicular content of caspase-3 protein, we observed that LA significantly increased the level of the active form. The co-injection of Ant interfered with this effect and Ant alone significantly decreased caspase-3 cleavage. IHC analyses corroborated these data. Notably, while LA increased caspase-3 activity levels, Ant decreased them when compared to controls. In follicles obtained from LA-treated rats, cleavage of PARP (a substrate of caspase-3) from the intact 113-kDa protein showed a significant enhancement in an 85-kDa fragment. The co-injection of Ant interfered with this effect. Ant alone significantly decreased PARP cleavage as compared to controls. We conclude that the decrease in VEGF-A, its receptor Flk-1/KDR, and ANPT-1 produced by the administration of GnRH-1 agonist is one of the mechanisms involved in ovarian cell apoptosis. This suggests an intraovarian role of an endogenous GnRH-like peptide in gonadotropin-induced follicular development. © 2007 Wiley-Liss, Inc. |l eng | |
| 593 | |a Instituto de Biología Y Mediana Experimental (IBYME)-CONICET, Departamento de Fisiología, Universidad de Buenos Aires, Buenos Aires, Argentina | ||
| 593 | |a Química Biológica, Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina | ||
| 593 | |a Institute de Biologia Y Medicina Experimental, Obligado 2490, 1428 Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a ANGIOGENESIS |
| 690 | 1 | 0 | |a APOPTOSIS |
| 690 | 1 | 0 | |a GNRH-I ANALOGS |
| 690 | 1 | 0 | |a OVARY |
| 690 | 1 | 0 | |a ANGIOPOIETIN RECEPTOR |
| 690 | 1 | 0 | |a CASPASE 3 |
| 690 | 1 | 0 | |a ITURELIX |
| 690 | 1 | 0 | |a LEUPRORELIN |
| 690 | 1 | 0 | |a VASCULOTROPIN A |
| 690 | 1 | 0 | |a VASCULOTROPIN RECEPTOR 2 |
| 690 | 1 | 0 | |a ANGIOGENESIS |
| 690 | 1 | 0 | |a APOPTOSIS |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a CONTROLLED STUDY |
| 690 | 1 | 0 | |a ENZYME ACTIVITY |
| 690 | 1 | 0 | |a FEMALE |
| 690 | 1 | 0 | |a IMMUNOCHEMISTRY |
| 690 | 1 | 0 | |a IN VIVO STUDY |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a OVARY |
| 690 | 1 | 0 | |a OVARY FOLLICLE |
| 690 | 1 | 0 | |a PRIORITY JOURNAL |
| 690 | 1 | 0 | |a PROTEIN EXPRESSION |
| 690 | 1 | 0 | |a RAT |
| 690 | 1 | 0 | |a ANGIOPOIETIN-1 |
| 690 | 1 | 0 | |a ANIMALS |
| 690 | 1 | 0 | |a APOPTOSIS |
| 690 | 1 | 0 | |a CASPASE 3 |
| 690 | 1 | 0 | |a ENZYME ACTIVATION |
| 690 | 1 | 0 | |a FEMALE |
| 690 | 1 | 0 | |a GONADOTROPIN-RELEASING HORMONE |
| 690 | 1 | 0 | |a INJECTIONS, SUBCUTANEOUS |
| 690 | 1 | 0 | |a LEUPROLIDE |
| 690 | 1 | 0 | |a NEOVASCULARIZATION, PHYSIOLOGIC |
| 690 | 1 | 0 | |a OLIGOPEPTIDES |
| 690 | 1 | 0 | |a OVARIAN FOLLICLE |
| 690 | 1 | 0 | |a OVARY |
| 690 | 1 | 0 | |a RATS |
| 690 | 1 | 0 | |a RATS, SPRAGUE-DAWLEY |
| 690 | 1 | 0 | |a RECEPTOR, TIE-2 |
| 690 | 1 | 0 | |a STRUCTURE-ACTIVITY RELATIONSHIP |
| 690 | 1 | 0 | |a VASCULAR ENDOTHELIAL GROWTH FACTOR A |
| 690 | 1 | 0 | |a VASCULAR ENDOTHELIAL GROWTH FACTOR RECEPTOR-2 |
| 690 | 1 | 0 | |a RATTUS |
| 700 | 1 | |a Irusta, G. | |
| 700 | 1 | |a Celín, A.R. | |
| 700 | 1 | |a Tesone, M. | |
| 773 | 0 | |d 2008 |g v. 75 |h pp. 623-631 |k n. 4 |p Mol. Reprod. Dev. |x 1040452X |t Molecular Reproduction and Development | |
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