Chemo- and stereoselective reduction of β-keto esters by spores and various morphological forms of Mucor rouxii

We report the efficient enantioselective reduction of a number of β-ketoesters by mycelial and yeast-like forms of the dimorphic fungus Mucor rouxii in a whole-cell process. Mycelial cells, grown in aerobiosis, were efficient in water, whereas the yeast-like cells, grown in anaerobic medium, were bo...

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Autor principal: Mangone, C.P
Otros Autores: N.Pereyra, E., Argimón, S., Moreno, S., Baldessari, A.
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 2002
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100 1 |a Mangone, C.P. 
245 1 0 |a Chemo- and stereoselective reduction of β-keto esters by spores and various morphological forms of Mucor rouxii 
260 |c 2002 
270 1 0 |m Baldessari, A.; Departamento de Quimica Organica, Facultad Ciencias Exactas Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina; email: alib@qo.fcen.uba.ar 
506 |2 openaire  |e Política editorial 
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504 |a Marcantoni, E., Alessandrini, S., Malavolta, M., Reverse stereochemical control in the presence of CeCl3 and TiCl4 in the Lewis acid mediated reduction of α-alkyl-β-ketoesters by metal hydrides. A general methodology for the diasteroselective synthesis of syn and anti α-alkyl-β-hydroxy esters (1999) J Org Chem, 64, pp. 1986-1992 
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504 |a Wong, C.H., Whitesides, G.M., Oxidoreductions (1994) Enzymes in Synthetic Organic Chemistry, pp. 131-194. , Balwin, Magnus, editors. Oxford: Elsevier Science Ltd 
504 |a Kitayama, T., Microbial asymmetric syntheses of 3-alkylphthalide derivatives (1997) Tetrahedron Asym, 22, pp. 3765-3774 
504 |a Panke, S., Wubbolts, M.G., Schmid, A., Withold, B., Production of enantiopure styrene oxide by recombinant Escherichia coli synthesizing a two component styrene monooxygenase (2000) Biotechnol Bioeng, 69, pp. 91-100 
504 |a Servi, S., Baker's yeast as a reagent in organic synthesis (1990) Synthesis, pp. 1-24 
504 |a Rotthaus, O., Krüger, D., Demuth, M., Schaffner, K., Reductions of keto- esters with baker's yeast in organic solvents. A comparison with the results in water (1997) Tetrahedron, 53, pp. 935-938 
504 |a Zaks, A., Klibanov, A.M., Enzyme-catalyzed processes in organic solvents (1985) Proc Natl Acad Sci USA, 82, pp. 3192-3196 
504 |a Terradas, F., Teston, H.M., Fitzpatrick, P.A., Klibanov, A.M., Marked dependence of enzyme prochiral selectivity on the solvent (1993) J Am Chem Soc, 115, pp. 390-396 
504 |a Hochuli, E., Taylor, K.E., Dutler, H., Dihydroxiacetone reductase from Mucor javanicus. 2. Identification of the physiological substrate and reactivity towards related compounds (1977) Eur J Biochem, 75, pp. 433-439 
504 |a Peters, J., Sypherd, P.S., Morphology-associated expression of nicotinamide adenine dinucleotide-dependent glutamate dehydrogenase in Mucor racemosus (1979) J Bacteriol, 137, pp. 1134-1139 
504 |a Shimizu, S., Hattori, S., Hata, H., Yamada, H., A novel fungal enzyme, NADPH-depedent carbonyl reductase, showing high specificity to conjugated polyketones. Purification and characterization (1988) Eur J Biochem, 174, pp. 37-44 
504 |a Zazueta-Sandoval, R., Gutiérrez-Corona, J.F., Developmental and environmental influences in the production of a single NAD-dependent fermentative alcohol dehydrogenase by the zygomycete Mucor rouxii (1999) Arch Microbiol, 172, pp. 280-286 
504 |a Haidle, C.W., Storck, R., Control of dimorphism in Mucor rouxii (1966) J Bacteriol, 92, pp. 1236-1244 
504 |a Bartnicki-García, S., Nickerson, W.J., Nutrition, growth, and morphogenesis of Mucor rouxii (1962) J Bacteriol, 83, pp. 841-858 
504 |a Seebach, D., Züger, M., Uber die depolymerisierung von Poly-(R)-3-hydroxy-buttersäureester (PHB) (1982) Helv Chim Acta, 65, pp. 495-504 
504 |a North, M., Baker's yeast reduction of β-ketoesters in petrol (1996) Tetrahedron Letters, 37, pp. 1699-1702 
504 |a Nakamura, K., Inoue, K., Ushio, L., Oka, S., Ohno, A., Stereochemical control on yeast reduction of α-ketoesters. Reduction by immobilized baker's yeast in hexane (1988) J Org Chem, 53, pp. 2589-2593 
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520 3 |a We report the efficient enantioselective reduction of a number of β-ketoesters by mycelial and yeast-like forms of the dimorphic fungus Mucor rouxii in a whole-cell process. Mycelial cells, grown in aerobiosis, were efficient in water, whereas the yeast-like cells, grown in anaerobic medium, were both efficient in water and in organic solvents. Almost 100% of conversion with 97% of enantiomeric excess of the (S) forms of the reduced β-ketoesters was obtained at 15 g biomass (wet weight)/mmol substrate in two hours. The fungal spores, which are the physiologically resistant form of the fungus, also catalyzed the reductive process efficiently and stereoselectively. The freeze-dried as well as the warm-air dried yeast-like cells, rehydrated in a small volume of water, maintained the same efficiency and selectivity of the reaction in organic solvents as the fresh biomass up to at least 4 months. © 2002 Elsevier Science Inc. All rights reserved.  |l eng 
536 |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas 
536 |a Detalles de la financiación: We thank UMYMFOR and LANAIS-EMAR for GC analysis and spectra. We are indebted to CONICET for partial financial support. We are grateful to Miguel Galvagno and Moisés Burachik for helpful discussions. 
593 |a Departamento De Química Orgánica, Facultad De Ciencias Exactas Y Naturales, Ciudad Universitaria, 1428, Buenos Aires, Argentina 
593 |a Departamento De Química Biológica, Facultad De Ciencias Exactas Y Naturales, Ciudad Universitaria, 1428, Buenos Aires, Argentina 
690 1 0 |a Β-KETOESTERS 
690 1 0 |a MUCOR ROUXII 
690 1 0 |a MYCELIAL 
690 1 0 |a SPORES 
690 1 0 |a YEAST 
690 1 0 |a BIOMASS 
690 1 0 |a CELLS 
690 1 0 |a ESTERS 
690 1 0 |a FUNGI 
690 1 0 |a ORGANIC SOLVENTS 
690 1 0 |a WATER 
690 1 0 |a YEAST 
690 1 0 |a STEREOSELECTIVITY 
690 1 0 |a REDUCTION 
690 1 0 |a ESTER DERIVATIVE 
690 1 0 |a ORGANIC SOLVENT 
690 1 0 |a WATER 
690 1 0 |a AEROBIC METABOLISM 
690 1 0 |a ARTICLE 
690 1 0 |a BACTERIAL SPORE 
690 1 0 |a BIOMASS 
690 1 0 |a CATALYSIS 
690 1 0 |a CONTROLLED STUDY 
690 1 0 |a ENANTIOMER 
690 1 0 |a ENANTIOSELECTIVITY 
690 1 0 |a FUNGAL CELL 
690 1 0 |a FUNGUS 
690 1 0 |a FUNGUS GROWTH 
690 1 0 |a FUNGUS SPORE 
690 1 0 |a MORPHOLOGY 
690 1 0 |a MUCOR 
690 1 0 |a MUCOR ROUXII 
690 1 0 |a MYCELIUM 
690 1 0 |a NONHUMAN 
690 1 0 |a PHYSIOLOGY 
690 1 0 |a REDUCTION 
690 1 0 |a REHYDRATION 
690 1 0 |a STEREOCHEMISTRY 
690 1 0 |a YEAST 
690 1 0 |a AMYLOMYCES ROUXII 
690 1 0 |a FUNGI 
690 1 0 |a MUCOR 
650 1 7 |2 spines  |a AEROBIOSIS 
700 1 |a N.Pereyra, E. 
700 1 |a Argimón, S. 
700 1 |a Moreno, S. 
700 1 |a Baldessari, A. 
773 0 |d 2002  |g v. 30  |h pp. 596-601  |k n. 5  |p Enzyme Microb. Technol.  |x 01410229  |w (AR-BaUEN)CENRE-1003  |t Enzyme and Microbial Technology 
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