Roscovitine inhibits ongoing DNA synthesis in human cervical cancer

The effect of roscovitine, a purine analogue and cyclin dependent kinase inhibitor, on DNA synthesis rate in tissue mini-units obtained from human cervical cancers was investigated. Roscovitine (100 μM) gave a DNA synthesis rate inhibition by 61% (P<0.0001; range 23-93%) within 30 min of incubati...

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Autor principal: Vitali, L.
Otros Autores: Yakisich, J.Sebastian, Vita, M.F, Fernandez, A., Settembrini, L., Siden, A., Cruz, M., Carminatti, H., Casas, O., Idoyaga Vargas, V.
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 2002
Acceso en línea:Registro en Scopus
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024 7 |2 scopus  |a 2-s2.0-18344377661 
024 7 |2 cas  |a Antineoplastic Agents; Cyclin-Dependent Kinases, EC 2.7.1.37; DNA, 9007-49-2; Enzyme Inhibitors; Purines; roscovitine 
040 |a Scopus  |b spa  |c AR-BaUEN  |d AR-BaUEN 
030 |a CALED 
100 1 |a Vitali, L. 
245 1 0 |a Roscovitine inhibits ongoing DNA synthesis in human cervical cancer 
260 |c 2002 
270 1 0 |m Idoyaga Vargas, V.; Inst. Invest. Bioquimi. Fund. Campo., FCEyN, UBA, Patricias Argentinas 435, (C. P. 1405) Buenos Aires, Argentina; email: vidoyagav@leloir.org.ar 
506 |2 openaire  |e Política editorial 
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504 |a Yakisich, J.S., Sidén, Å., Idoyaga Vargas, V., Eneroth, P., Cruz, M., Inhibition of DNA synthesis in human gliomas by roscovitine (1999) NeuroReport, 10, pp. 2563-2567 
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504 |a Funk, J.O., Waga, S., Harry, J.B., Espling, E., Stillman, B., Galloway, D.A., Inhibition of CDK activity and PCNA-dependent DNA replication by p21 is blocked by interaction with the HPV-16 E7 oncoprotein (1997) Oncogene, 11, pp. 2090-2100 
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504 |a Yakisich, J.S., Sidén, Å., Idoyaga Vargas, V., Eneroth, P., Cruz, M., Early inhibition of DNA synthesis in the developing rat cerebral cortex by the purine analogues olomoucine and roscovitine (1998) Biochem. Biophys. Res. Commun., 243, pp. 674-677 
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504 |a Bresnahan, W.A., Boldogh, I., Chi, P., Thompson, E.A., Albrecht, T., Inhibition of cellular Cdk2 activity blocks human cytomegalovirus replication (1997) Virology, 231, pp. 239-247 
504 |a Wang, D., De la Fuente, C., Deng, L., Wang, L., Zilberman, I., Eadie, C., Healey, M., Kashanchi, F., Inhibition of human immunodeficiency virus type 1 transcription by chemical cyclin-dependent kinase inhibitors (2001) J. Virol., 75, pp. 7266-7279 
520 3 |a The effect of roscovitine, a purine analogue and cyclin dependent kinase inhibitor, on DNA synthesis rate in tissue mini-units obtained from human cervical cancers was investigated. Roscovitine (100 μM) gave a DNA synthesis rate inhibition by 61% (P<0.0001; range 23-93%) within 30 min of incubation. This inhibitory effect was concentration-dependent. The results suggest that the inhibition of tumor DNA synthesis rate is due to a direct effect on the DNA synthesis machinery via presently unknown mechanisms. In addition, the potential application of CDKs inhibitors as preventive agents is discussed. © 2002 Elsevier Science Ireland Ltd. All rights reserved.  |l eng 
536 |a Detalles de la financiación: Universidad de Buenos Aires 
536 |a Detalles de la financiación: Minerva Foundation 
536 |a Detalles de la financiación: Karolinska Institutet, KI 
536 |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas 
536 |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas 
536 |a Detalles de la financiación: This study was supported by grants from the Consejo Nacional de Investigaciones CientÍficas y Técnicas (CONICET) as well as University of Buenos Aires, the Avanzar Foundation, the Karolinska Institute, the Minerva Foundation, and the Swedish Medical Association. The access to resources at the Clinical Research Center, Novum, is gratefully acknowledged. 
593 |a Instituto de Investigaciones Bioquimicas Fundacion Campomar, FCEyN, UBA, Patricias Argentinas 435, Buenos Aires, Argentina 
593 |a Applied Biochemistry, Clinical Research Center, Karolinska Institute, Novum, Huddinge, Sweden 
593 |a Department of Neurology, Huddinge University Hospital, Karolinska Institute, Huddinge, Sweden 
593 |a Hospital Municipal de OncologÍa Marie Curie, Buenos Aires, Argentina 
593 |a Instituto de Terapia Radiante, La Plata, Argentina 
690 1 0 |a CERVICAL CANCER 
690 1 0 |a CYCLIN DEPENDENT KINASES 
690 1 0 |a DNA SYNTHESIS 
690 1 0 |a ROSCOVITINE 
690 1 0 |a ROSCOVITINE 
690 1 0 |a ADULT 
690 1 0 |a AGED 
690 1 0 |a ARTICLE 
690 1 0 |a CANCER CHEMOTHERAPY 
690 1 0 |a CANCER STAGING 
690 1 0 |a CELL PROLIFERATION 
690 1 0 |a CLINICAL ARTICLE 
690 1 0 |a CONCENTRATION RESPONSE 
690 1 0 |a CONTROLLED STUDY 
690 1 0 |a DNA SYNTHESIS 
690 1 0 |a DNA SYNTHESIS INHIBITION 
690 1 0 |a DRUG EFFECT 
690 1 0 |a FEMALE 
690 1 0 |a HUMAN 
690 1 0 |a HUMAN TISSUE 
690 1 0 |a PRIORITY JOURNAL 
690 1 0 |a UTERINE CERVIX CANCER 
690 1 0 |a ADULT 
690 1 0 |a AGED 
690 1 0 |a ANTINEOPLASTIC AGENTS 
690 1 0 |a CYCLIN-DEPENDENT KINASES 
690 1 0 |a DNA 
690 1 0 |a DOSE-RESPONSE RELATIONSHIP, DRUG 
690 1 0 |a ENZYME INHIBITORS 
690 1 0 |a FEMALE 
690 1 0 |a HUMANS 
690 1 0 |a MIDDLE AGED 
690 1 0 |a PURINES 
690 1 0 |a TIME FACTORS 
690 1 0 |a UTERINE CERVICAL NEOPLASMS 
700 1 |a Yakisich, J.Sebastian 
700 1 |a Vita, M.F. 
700 1 |a Fernandez, A. 
700 1 |a Settembrini, L. 
700 1 |a Siden, A. 
700 1 |a Cruz, M. 
700 1 |a Carminatti, H. 
700 1 |a Casas, O. 
700 1 |a Idoyaga Vargas, V. 
773 0 |d 2002  |g v. 180  |h pp. 7-12  |k n. 1  |p Cancer Lett.  |x 03043835  |w (AR-BaUEN)CENRE-4108  |t Cancer Letters 
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856 4 0 |u https://hdl.handle.net/20.500.12110/paper_03043835_v180_n1_p7_Vitali  |y Handle 
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