An antiviral principle present in a purified fraction from Melia azedarach L. leaf aqueous extract restrains herpes simplex virus type 1 propagation

Meliacine (MA), an antiviral principle isolated from leaves of Melia azedarach L., exhibits potent antiviral activity against herpes simplex virus type 1 (HSV-1) by inhibiting specific infected-cell polypeptides (ICPs) produced late in infection. Some of these are involved in DNA synthesis and in th...

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Autor principal: Alché, L.E
Otros Autores: Barquero, A.A, Sanjuan, N.A, Coto, C.E
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 2002
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024 7 |2 scopus  |a 2-s2.0-0036286899 
024 7 |2 cas  |a Antiviral Agents; DNA, Viral; Plant Extracts; Viral Proteins 
040 |a Scopus  |b spa  |c AR-BaUEN  |d AR-BaUEN 
030 |a PHYRE 
100 1 |a Alché, L.E. 
245 1 3 |a An antiviral principle present in a purified fraction from Melia azedarach L. leaf aqueous extract restrains herpes simplex virus type 1 propagation 
260 |c 2002 
270 1 0 |m Alché, L.E.; Lab. de Virología, Depto. de Quimica Biologica, Ciudad Universitaria, 1428 Buenos Aires, Argentina; email: lalche@qb.fcen.uba.ar 
506 |2 openaire  |e Política editorial 
504 |a Alché, L.E., Berra, A., Veloso, M.J., Coto, C.E., Treatment with meliacine, a plant derived antiviral, inhibits herpetic stromal queratitis in mice (2000) J Med Virol, 61 (4), pp. 474-480 
504 |a Alrabiah, F.A., Sacks, S.L., New antiherpes virus agents: Their targets and therapeutic potential (1996) Drugs, 52, pp. 17-32 
504 |a Andrei, G., Couto, A.S., Lederkremer, R.M., Coto, C.E., Purification and partial characterization of an antiviral active peptide from Melia azedarach L (1994) Antiviral Chem Chemother, 5, pp. 105-110 
504 |a Barquero, A.A., Alché, L.E., Coto, C.E., Antiviral activity of meliacine on the replication of a thymidine kinase-deficient mutant of herpes simplex virus type 1 alone and in combination with acyclovir (1997) Int J Antimicrob Agents, 9, pp. 49-55 
504 |a Benencia, F., Courreges, M.C., Coulombié, F.C., Antiviral activity of crude polysaccharides from Trichilia glabra leaves (1997) Fitoterapia, 68, pp. 173-175 
504 |a Campadelli-Fiume, G., Farabegoli, F., Gaeta, S., Roizman, B., Origin of unenveloped capsids in the cytoplasm of cells infected with herpes simplex virus 1 (1991) J Virol, 65, pp. 1589-1595 
504 |a Cheung, P., Banfield, B.W., Tufaro, F., Brefeldin A arrests the maturation and egress of herpes simplex virus particles during infection (1991) J Virol, 65, pp. 1893-1904 
504 |a Cordell, G.A., Changing strategies in natural products chemistry (1995) Phytochemistry, 40, pp. 1585-1612 
504 |a Córdoba, M.A., Coto, C.E., Damonte, E.B., Virucidal activity in aqueous extracts obtained from Cedrela tubiflora leaves (1991) Phytother Res, 5, pp. 250-253 
504 |a Darby, G., In search of the perfect antiviral (1995) Antiviral Chem Chemother, 6, pp. 54-63 
504 |a Hirsch, M.S., Kaplan, J.C., D'Aquila, R.T., Antiviral agents (1996) Virology, 3rd edn, pp. 436-445. , Fields BN, Knipe DM, Howley PM (eds). Lippincott-Raven: Philadelphia 
504 |a Johnson, P.A., Best, M.G., Friedmann, T., Parris, D.S., Isolation of a herpes simplex virus type 1 mutant deleted for the essential UL42 gene and characterization of its null phenotype (1991) J Virol, 65, pp. 700-710 
504 |a Koyama, A.H., Uchida, T., Quantitative studies on the maturation process of herpes simplex virus type 1 in Vero cells (1988) Virus Res, 10, pp. 281-285 
504 |a Mahmood, N., PIacente, S., Burke, A., Khan, A., Pizza, C., Constituents of Cuscuta reflexa are anti-HIV agents (1997) Antiviral Chem Chemother, 8, pp. 70-74 
504 |a Ngan, F., Chang, R.S., Tabba, H.D., Smith, K.M., Isolation, purification and partial characterization of an active anti-HIV compound from the Chinese medicinal herb Viola yedoensis (1988) Antiviral Res, 10, pp. 107-116 
504 |a Prusoff, W.H., Lin, T.-S., August, E.M., Overview of molecular aspects of antiviral drug action and design (1990) Pharmacol Ther, (SUPPL.), pp. 11-24. , Chapter 2 
504 |a Souza Brito, A.R.M., How to study the pharmacology of medicinal plants in underdeveloped countries (1996) J Ethnopharmacol, 54, pp. 131-138 
504 |a Villamil, S.M., Alché, L.E., Coto, C.E., Inhibition of herpes simplex virus type 1 multiplication by meliacine, a peptide of plant origin (1995) Antiviral Chem Chemother, 6, pp. 239-244 
504 |a Wachsman, M.B., Martino, V., Coto, C.E., Search for antiviral activity in higher plant extracts (1988) Fitoterapia, 59, pp. 422-424 
504 |a Waxman, L., Darke, P., The herpesvirus proteases as targets for antiviral chemotherapy (2000) Antiviral Chem Chemother, 11, pp. 1-22 
504 |a Xu Ze-Qi, Kern, E.R., Westbrook, L., Plant-derived and semi-synthetic calanolide compounds with in vitro activity against both human immunodeficiency virus type 1 and human cytomegalovirus (2000) Antiviral Chem Chemother, 11, pp. 23-29 
520 3 |a Meliacine (MA), an antiviral principle isolated from leaves of Melia azedarach L., exhibits potent antiviral activity against herpes simplex virus type 1 (HSV-1) by inhibiting specific infected-cell polypeptides (ICPs) produced late in infection. Some of these are involved in DNA synthesis and in the assembly of nucleocapsids. The present report provides additional evidence to elucidate the mode of action of MA against HSV-1. Time-of-addition experiments confirmed that MA affects a late event in the multiplication cycle of HSV-1. We showed that MA diminished the synthesis of viral DNA and inhibited the spread of infectious viral particles when HSV-1 that expresses β-galactosidase activity was used. In addition, the lack of a protein with an apparent MW of 55 KD was detected in MA-treated cell extracts. Ultrastructural analysis of infected cells showed that, in the case of MA treatment, a large number of unenveloped nucleocapsids accumulated in the cytoplasm and a minor proportion of mature virus was found in cytoplasmic vesicles. These findings suggest that MA exerts an antiviral action on both the synthesis of viral DNA and the maturation and egress of HSV-1 during the infection of Vero cells. Copyright © 2002 John Wiley & Sons, Ltd.  |l eng 
593 |a Laboratory of Virology, University of Buenos Aires, Ciudad Universitaria, 1428 Buenos Aires, Argentina 
593 |a Department of Microbiology, School of Medicine, University of Buenos Aires, Paraguay 2155, 1113 Buenos Aires, Argentina 
593 |a Lab. de Virología, Depto. de Química Biológica Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, 1428 Buenos Aires, Argentina 
593 |a CONICET, Argentina 
690 1 0 |a ANTIVIRAL 
690 1 0 |a HERPES SIMPLEX VIRUS TYPE 1 
690 1 0 |a MELIA AZEDARACH L. 
690 1 0 |a MELIACINE 
690 1 0 |a ANTIVIRUS AGENT 
690 1 0 |a BETA GALACTOSIDASE 
690 1 0 |a MELIACINE 
690 1 0 |a POLYPEPTIDE 
690 1 0 |a UNCLASSIFIED DRUG 
690 1 0 |a ANIMAL CELL 
690 1 0 |a ANTIVIRAL ACTIVITY 
690 1 0 |a AQUEOUS SOLUTION 
690 1 0 |a ARTICLE 
690 1 0 |a CONTROLLED STUDY 
690 1 0 |a CYTOPLASM 
690 1 0 |a CYTOPLASM VESICLE 
690 1 0 |a DNA CLEAVAGE 
690 1 0 |a DNA PACKAGING 
690 1 0 |a DNA SYNTHESIS 
690 1 0 |a DRUG MECHANISM 
690 1 0 |a DRUG SYNTHESIS 
690 1 0 |a ENZYME ACTIVITY 
690 1 0 |a HERPES SIMPLEX VIRUS 1 
690 1 0 |a MEDICINAL PLANT 
690 1 0 |a MELIA AZEDARACH 
690 1 0 |a MOLECULAR WEIGHT 
690 1 0 |a NONHUMAN 
690 1 0 |a PLANT LEAF 
690 1 0 |a TIME 
690 1 0 |a VERO CELL 
690 1 0 |a VIRUS ENVELOPE 
690 1 0 |a VIRUS INHIBITION 
690 1 0 |a VIRUS NUCLEOCAPSID 
690 1 0 |a VIRUS PARTICLE 
690 1 0 |a VIRUS REPLICATION 
690 1 0 |a ANIMALS 
690 1 0 |a ANTIVIRAL AGENTS 
690 1 0 |a CELL TRANSFORMATION, VIRAL 
690 1 0 |a CERCOPITHECUS AETHIOPS 
690 1 0 |a DNA, VIRAL 
690 1 0 |a HERPESVIRUS 1, HUMAN 
690 1 0 |a MELIACEAE 
690 1 0 |a PLANT EXTRACTS 
690 1 0 |a PLANT LEAVES 
690 1 0 |a VERO CELLS 
690 1 0 |a VIRAL PROTEINS 
690 1 0 |a VIRUS REPLICATION 
690 1 0 |a HUMAN HERPESVIRUS 1 
690 1 0 |a MELIA AZEDARACH 
690 1 0 |a SIMPLEXVIRUS 
650 1 7 |2 spines  |a VIRUS DNA 
700 1 |a Barquero, A.A. 
700 1 |a Sanjuan, N.A. 
700 1 |a Coto, C.E. 
773 0 |d 2002  |g v. 16  |h pp. 348-352  |k n. 4  |p Phytother. Res.  |x 0951418X  |t Phytotherapy Research 
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