Seasonal changes in the activity of cytochrome P450C17 from the testis of Bufo arenarum
In Bufo arenarum, the biosynthesis of testosterone and 5α-dihydrotestosterone takes place through a complete 5-ene pathway, 5-androsten-3β,17β-diol being the immediate precursor of testosterone. Besides androgens, testes are able to synthesise 5α-pregnan-3,20-dione and several 3α and 20α reduced der...
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2002
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| LEADER | 13614caa a22012137a 4500 | ||
|---|---|---|---|
| 001 | PAPER-5114 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203444.0 | ||
| 008 | 190411s2002 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0036937756 | |
| 024 | 7 | |2 cas |a steroid 17alpha monooxygenase, 9029-67-8; testosterone, 58-22-0; Immune Sera; Steroid 17-alpha-Hydroxylase, EC 1.14.99.9; Testosterone, 58-22-0 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a JCPBD | ||
| 100 | 1 | |a Solari, J.J.F. | |
| 245 | 1 | 0 | |a Seasonal changes in the activity of cytochrome P450C17 from the testis of Bufo arenarum |
| 260 | |c 2002 | ||
| 270 | 1 | 0 | |m Ceballos, N.R.; PRHOM-CONICET/Lab. de Endocrinol. C., Depto. de Biodiversidad y Biol. Exp., Universidad de Buenos Aires, Buenos Aires, Argentina; email: nceballo@bg.fcen.uba.ar |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Ames, B.N., Assay of inorganic phosphate, total phosphate and phosphatases (1966) Methods Enzymol, 8, pp. 115-118 | ||
| 504 | |a Aronson, N.N., Touster, O., Isolation of rat liver plasma membrane fragments in isotonic sucrose (1974) Methods Enzymol, 31, pp. 90-94 | ||
| 504 | |a Bhasker, C.R., Adler, B.S., Dee, A., John, M.E., Kagimoto, M., Zuber, M.X., Ahlgren, R., Waterman, M.R., Structural characterization of the bovine CYP17 (17α-hydroxylase) gene (1989) Arch Biochem Biophys, 271, pp. 479-487 | ||
| 504 | |a Canick, J., Fox, C., Callard, G., Studies on cytochrome P-450-dependent microsomal enzymes of testicular androgen and estrogen biosynthesis in a urodele amphibian, Necturus (1984) J Steroid Biochem, 21, pp. 15-20 | ||
| 504 | |a Canosa, L.F., Ceballos, N.R., Effects of different steroid-biosynthesis inhibitors on the testicular steroidogenesis of the toad Bufo arenarum (2001) J Comp Physiol B, 171, pp. 519-526 | ||
| 504 | |a Canosa, L.F., Ceballos, N.R., Seasonal changes in testicular steroidogenesis in the toad Bufo arenarum H (2002) Gen Comp Endocrinol, 125, pp. 426-434 | ||
| 504 | |a Canosa, L.F., Pozzi, A.G., Ceballos, N.R., Pregnenolone and Progesterone metabolism by the testes of Bufo arenarum (1998) J Comp Physiol B, 168, pp. 491-496 | ||
| 504 | |a Chung, B.C., Picado Leonard, J., Haniu, M., Bienkowsky, M., Hall, P.F., Shively, J.E., Miller, W.L., Cytochrome P-450-c17 (steroid 17α-hydroxylase/17,20-liase): Cloning of human adrenal and testis cDNAs indicates the same gene is expressed in both tissues (1987) Proc Natl Acad Sci USA, 84, pp. 407-411 | ||
| 504 | |a Dalla Valle, L., Ramina, A., Vianello, S., Belvedere, P., Colombo, L., Kinetic analysis of duodenal and testicular cytochrome P450c17 in the rat (1996) J Steroid Biochem Mol Biol, 58, pp. 577-584 | ||
| 504 | |a Fevold, H.R., Lorence, M.C., McCarthy, J.L., Trant, J.M., Kagimoto, M., Waterman, M.R., Mason, J.I., Rat P450 (17 alpha) from testis: Characterization of a full length cDNA encoding a unique steroid hydroxylase capable of catalyzing delta 4- and delta 5-steroid-17,20-lyase reactions (1989) Mol Endocrinol, 3, pp. 968-975 | ||
| 504 | |a Frye, C.A., Vongher, J.M., Progesterone has rapid and membrane effects in the facilitation of female mouse sexual behaviour (1999) Brain Res, 815, pp. 259-269 | ||
| 504 | |a Frye, C.A., Bayon, L.E., Pursnani, N.K., Purdy, R.H., The neurosteroids, progesterone and 3α,5α-THP, enhance sexual motivation, receptivity and proceptivity in female rat (1998) Brain Res, 808, pp. 72-83 | ||
| 504 | |a Guarino, F.M., Di Fiore, M.M., Caputo, V., Angelini, F., Iela, L., Rastogi, R.K., Seasonal analysis of the reproductive cycle in tow wild populations of Rana italica Dubois 1985 (1993) Anim Biol, 2, pp. 25-43 | ||
| 504 | |a Hall, P.F., Testicular steroid synthesis: Organization and regulation (1994) The Physiology of Reproduction, 1, pp. 1335-1365. , Knobil E, Neill JD (eds). Raven, New York | ||
| 504 | |a D'Istria, M., Delrio, G., Botte, V., Chieffi, G., Radioimmunoassay of testosterone, 17β-oestradiol and oestrone in the male and female plasma of Rana esculenta during sexual cycle (1974) Steroids Lipids Res, 5, pp. 42-48 | ||
| 504 | |a Itoh, M., Ishii, S., Changes in plasma levels of gonadotropins and sex steroids in the toad, Bufo japonicus, in association with behaviour during the breading season (1990) Gen Comp Endocrinol, 80, pp. 451-464 | ||
| 504 | |a Itoh, M., Inoue, M., Ishii, S., Annual cycle of pituitary and plasma gonadotropins and sex steroids in a wild population of the toad, Bufo japonicus (1990) Gen Comp Endocrinol, 78, pp. 242-253 | ||
| 504 | |a Kime, D.E., Comparative aspects of testicular androgen biosynthesis in nonmammalian vertebrates (1980) Steroids and their Mechanism of Actions in Nonmammalian Vertebrates, pp. 17-31. , Delrio G, Brachet J (eds). Raven, New York | ||
| 504 | |a Kime, D.E., Hews, E.A., Androgen biosynthesis in vitro by testes from amphibia (1978) Gen Comp Endocrinol, 35, pp. 280-288 | ||
| 504 | |a Kobayashi, T., Sakai, N., Adachi, S., Asahina, K., Iwasawa, H., Nagahama, Y., 17-alpha,20-alpha-Dihydroxy-4-pregnen-3-one is the naturally occurring spermiation-inducing hormone in the testis of a frog, Rana nigromaculata (1993) Endocrinology, 133, pp. 321-327 | ||
| 504 | |a Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J., Protein measurement with the Folin phenol reagent (1951) J Biol Chem, 193, pp. 265-275 | ||
| 504 | |a Mensah-Nyagan, A.G., Do-Rego, J.L., Beaujean, D., Luu-The, V., Pelletier, G., Vaudry, H., Neurosteroids: Expression of steroidogenic enzymes and regulation of steroid biosynthesis in central nervous system (1999) Pharmacol Rev, 51, pp. 63-81 | ||
| 504 | |a Mensah-Nyagan, A.G., Beaujean, D., Do-Rego, J.L., Mathieu, M., Vallarino, M., Luu-The, V., Pelletier, G., Vaudry, H., In vivo evidence for the production of sulphated steroids in the frog brain (2000) Comp Biochem Physiol B, 126, pp. 213-219 | ||
| 504 | |a Müller, C.H., In vitro stimulation of 5 alpha-dihydrotestosterone and testosterone secretion from bullfrog testis by non-mammalian and mammalian gonadotropins (1977) Gen Comp Endocrinol, 33, pp. 109-121 | ||
| 504 | |a Nakajin, S., Hall, P.F., Microsomal cytochrome P-450 from neonatal pig testis: Purification and properties of a C21 steroid side-chain cleavage system (17-hydroxylase and C17,20 lyase) (1981) J Biol Chem, 256, pp. 3871-3876 | ||
| 504 | |a Payne, A.H., Hormonal regulation of cytochrome P450 enzymes, cholesterol side-chain cleavage and 17α-hydroxylase/C17-20 lyase in Leydig cells (1990) Biol Reprod, 42, pp. 399-404 | ||
| 504 | |a Pennington, R.J., Biochemistry of dystrophic muscle: Mitochondrial succinate-tetrazolium reductase and adenosine triphosphatase (1961) Biochem J, 80, pp. 649-654 | ||
| 504 | |a Perrin, A., Defaye, G., Guidicelli, C., Chambaz, E.M., Purification and properties of steroid 17α-hydroxylase from calf testis (1991) J Steroid Biochem Mol Biol, 40, pp. 431-435 | ||
| 504 | |a Perrin, A., Chambaz, E.M., Defaye, G., Modulation of hydroxylase and lyase activities of bovine cytochrome P-45017α in adrenal and testicular microsomes by a tissue-specific local membrane environment (1995) J Steroid Biochem Mol Biol, 54, pp. 121-129 | ||
| 504 | |a Pozzi, A.G., Ceballos, N.R., Human chorionic gonadotropin-induced spermiation in Bufo arenarum is not mediated by steroid biosynthesis (2000) Gen Comp Endocrinol, 119, pp. 164-171 | ||
| 504 | |a Pozzi, A.G., Lantos, C.P., Ceballos, N.R., Subcellular localization of 3 beta hydroxysteroid dehydrogenase isomerase in testis of Bufo arenarum H (1997) Gen Comp Endocrinol, 106, pp. 400-406 | ||
| 504 | |a Pozzi, A.G., Canosa, L.F., Calvo, J.C., Ceballos, N.R., Kinetic properties of mitochondrial and microsomal 3β-hydroxysteroid dehydrogenase-isomerase from testis of Bufo arenarum (2000) J Steroid Biochem Mol Biol, 73, pp. 257-264 | ||
| 504 | |a Rastogi, R.K., Iela, L., Saxena, P.K., Chieffi, G., The control of spermatogenesis in the green frog, Rana esculenta (1976) J Exp Zool, 196, pp. 151-166 | ||
| 504 | |a Specker, J.L., Moore, F.L., Annual cycle of plasma androgens and testicular composition in the rough-skinned newt, Taricha granulosa (1980) Gen Comp Endocrinol, 42, pp. 297-303 | ||
| 504 | |a Stacey, N.E., Cardwell, J.R., Liley, N.R., Scott, A.P., Sorensen, P.W., Hormone as sex pheromones in fish (1994) Perspectives in Comparative Endocrinology, pp. 438-448. , Davey KG, Peter RE, Tobe SS (eds). NSERC, Ottawa | ||
| 504 | |a Takasc, M., Ukena, K., Yamazaki, T., Kominami, S., Tsutsui, K., Pregnenolone, pregnenolone sulphate and cytochrome P450 side-chain cleavage enzyme in the amphibian brain and the seasonal changes (1999) Endocrinology, 140, pp. 1936-1944 | ||
| 504 | |a Wada, M., Wingfield, J.C., Gorbman, A., Correlation between blood level of androgens and sexual behaviour in male leopard frogs, Rana pipiens (1976) Gen Comp Endocrinol, 29, pp. 72-77 | ||
| 504 | |a Woodley, S.K., Plasma androgen levels, spermatogenesis, and secondary sexual characteristics in two species of plethodontid salamanders with dissociated reproductive patterns (1994) Gen Comp Endocrinol, 96, pp. 206-214 | ||
| 504 | |a Wright, R.S., A reagent for non-destructive localization of steroids and some other lipophilic materials on silica gel thin layer chromatograms (1971) J Chromatogr, 59, pp. 220-221 | ||
| 520 | 3 | |a In Bufo arenarum, the biosynthesis of testosterone and 5α-dihydrotestosterone takes place through a complete 5-ene pathway, 5-androsten-3β,17β-diol being the immediate precursor of testosterone. Besides androgens, testes are able to synthesise 5α-pregnan-3,20-dione and several 3α and 20α reduced derivatives. During the breeding season, steroid biosynthesis turns from androgen to C21-steroid production. As a consequence, the cytochrome P450 17-hydroxylase, C17,20-lyase (CypP450c17) could be a key enzyme in that metabolic shift. The present study demonstrates that in testes of B. arenarum, CypP450c17 co-localises with glucose-6-phosphatase in the microsomal fraction. CypP450c17 possesses more affinity for pregnenolone than for progesterone in both non-reproductive (Km=43.764±4.63 nM and 2,170±630 nM, respectively) and reproductive (Km=37.46± 4.19 nM and 3,060±190 nM, respectively) seasons. These results could explain the predominance of the 5-ene pathway for testosterone biosynthesis. Toad CypP450c17 activity is higher in the non-reproductive period than the reproductive period, suggesting that this enzyme is an important factor in toad steroidogenic changes. Animals in reproductive conditions showed a significant reduction in circulating androgens. This is in agreement with the decrease in Vmax of cytochrome P450 17-hydroxylase activity, enhancing the physiological relevance of these in vitro results. |l eng | |
| 536 | |a Detalles de la financiación: Universidad de Buenos Aires | ||
| 536 | |a Detalles de la financiación: National Council for Scientific Research | ||
| 536 | |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas | ||
| 536 | |a Detalles de la financiación: Acknowledgements This work was supported by grants from the National Research Council of Argentina (CONICET) to its Prog-rama de Regulación Hormonal y Metabólica (PRHOM), and the University of Buenos Aires. The authors wish to thank Dr. Edu-ardo Cozza for critical revision of the manuscript. The experiments comply with the ‘‘Principles of animal care’’, publication no.: 86– 23, revised 1985, of the National Institute of Health and also with Argentinean laws. | ||
| 593 | |a PRHOM-CONICET/Lab. de Endocrinol. C., Depto. de Biodiversidad y Biol. Exp., Universidad de Buenos Aires, Buenos Aires, Argentina | ||
| 593 | |a Depto. de Biodiversidad y Biol. Exp., Fac. de Ciencias Exactas y Naturales, Ciudad Universitaria, C1428EHA Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a CYTOCHROME P450C17 |
| 690 | 1 | 0 | |a ENZYME ACTIVITY |
| 690 | 1 | 0 | |a KINETIC PARAMETERS |
| 690 | 1 | 0 | |a SEASONAL CHANGES |
| 690 | 1 | 0 | |a TOAD TESTIS |
| 690 | 1 | 0 | |a ANTISERUM |
| 690 | 1 | 0 | |a STEROID 17ALPHA MONOOXYGENASE |
| 690 | 1 | 0 | |a TESTOSTERONE |
| 690 | 1 | 0 | |a ANIMAL |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a BLOOD |
| 690 | 1 | 0 | |a CELL FRACTIONATION |
| 690 | 1 | 0 | |a KINETICS |
| 690 | 1 | 0 | |a MALE |
| 690 | 1 | 0 | |a METABOLISM |
| 690 | 1 | 0 | |a SEASON |
| 690 | 1 | 0 | |a TESTIS |
| 690 | 1 | 0 | |a TISSUE DISTRIBUTION |
| 690 | 1 | 0 | |a TOAD |
| 690 | 1 | 0 | |a ANIMALS |
| 690 | 1 | 0 | |a BUFO ARENARUM |
| 690 | 1 | 0 | |a IMMUNE SERA |
| 690 | 1 | 0 | |a KINETICS |
| 690 | 1 | 0 | |a MALE |
| 690 | 1 | 0 | |a SEASONS |
| 690 | 1 | 0 | |a STEROID 17-ALPHA-HYDROXYLASE |
| 690 | 1 | 0 | |a SUBCELLULAR FRACTIONS |
| 690 | 1 | 0 | |a TESTIS |
| 690 | 1 | 0 | |a TESTOSTERONE |
| 690 | 1 | 0 | |a TISSUE DISTRIBUTION |
| 700 | 1 | |a Pozzi, A.G. | |
| 700 | 1 | |a Ceballos, N.R. | |
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