Antibody response to a viral transcriptional regulator
The E2 transcriptional activator of the human papillomavirus regulates the expression of most viral transcripts. Its binding to specific target DNA sequences involves large conformational changes in the interacting macromolecules. The high stability of the E2:DNA complex prompted us to analyze the r...
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| Otros Autores: | , , |
| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2003
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| LEADER | 07176caa a22010577a 4500 | ||
|---|---|---|---|
| 001 | PAPER-5077 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203442.0 | ||
| 008 | 190411s2003 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0037448443 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a FEBLA | ||
| 100 | 1 | |a Cerutti, M.L. | |
| 245 | 1 | 0 | |a Antibody response to a viral transcriptional regulator |
| 260 | |b Elsevier |c 2003 | ||
| 270 | 1 | 0 | |m Goldbaum, F.A.; F. I. L. IIBBA-CONICET, IIB-FCEN-UBA, Av. Patricias Argentinas 435, Buenos Aires 1405, Argentina; email: fgoldbaum@leloir.org.ar |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a McBride, A., Romanczuk, H., Howley, P.M., (1991) J. Biol. Chem., 266, pp. 18411-18414 | ||
| 504 | |a Lima, L.M., Prat Gay G. de, (1997) J. Biol. Chem., 272, pp. 19295-19303 | ||
| 504 | |a Ferreiro, D.U., Lima, L.M.T.R., Alonso, L.G., Goldbaum, F.A., Prat Gay G. de, (2000) Biochemistry, 39, pp. 14692-14701 | ||
| 504 | |a Moore, J.P., Trkola, A., Dragic, T., (1997) Curr. Opin. Immunol., 9, pp. 551-562 | ||
| 504 | |a Cerutti, M.L., Centeno, J.M., Goldbaum, F.A., Prat Gay G. de, (2001) J. Biol. Chem., 276, pp. 12769-12773 | ||
| 504 | |a Ulrich, J.T., Myers, K.R., (1995) Vaccine Design: The Subunit and Adjuvant Approach, 6, pp. 495-524. , Powell and Newman, Eds., Plenum Press, New York | ||
| 504 | |a Vogel, F.R., Powell, M.F., (1995) Vaccine Design: The Subunit and Adjuvant Approach, 6, pp. 141-228. , Powell and Newman, Eds., Plenum Press, New York | ||
| 504 | |a Galfré, G.A., Milstein, C., (1981) Methods Enzymol., 73, pp. 3-46 | ||
| 504 | |a Goldbaum, F.A., Cauerhff, A., Velikovsky, C.A., Llera, A.S., Riottot, M., Poljak, R.J., (1999) J. Immunol., 162, pp. 6040-6045 | ||
| 504 | |a Hassen, E., Chaieb, A., Bouaouina, N., Khairi, H., Remadi, S., Chouchane, L., (2001) Mod. Asp. Immunobiol., 2, pp. 8-12 | ||
| 504 | |a Foguel, D., Silva, J.L., Prat Gay G. de, (1998) J. Biol. Chem., 273, pp. 9050-9057 | ||
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| 504 | |a Orten, D.J., Strawhecker, J.M., Sanderson, S.D., Huang, D., Prystowsky, M.B., Hinrichs, S.H., (1994) J. Biol. Chem., 269, pp. 32254-32263 | ||
| 504 | |a Kim, S.S., Tam, J.K., Wang, A.F., Hegde, R.S., (2000) J. Biol. Chem., 275, pp. 31245-31254 | ||
| 504 | |a Gauthier, J.-M., Dillner, J., Yaniv, M., (1991) Nucleic Acids Res., 19, pp. 7073-7079 | ||
| 504 | |a Thompson, N.E., Strasheim, L.A., Nolan, K.M., Burgess, R.R., (1995) J. Biol. Chem., 270, pp. 4735-4740 | ||
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| 520 | 3 | |a The E2 transcriptional activator of the human papillomavirus regulates the expression of most viral transcripts. Its binding to specific target DNA sequences involves large conformational changes in the interacting macromolecules. The high stability of the E2:DNA complex prompted us to analyze the role of macromolecular interactions and adjuvant emulsions in the appearance of conformation-specific antibodies. We demonstrate that immunization with free or DNA-complexed E2 emulsified in an oil-in-water adjuvant elicits a humoral response shifted to the recognition of discontinuous epitopes. Epitope mapping and functional analysis of the generated anti-E2 mAbs reveals that two separate antibodies populations can be obtained: those able to form a stable ternary complex with protein and DNA, and those which recognize the DNA-binding surface of the transcription factor, interfering with E2 binding to DNA. © 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved. |l eng | |
| 536 | |a Detalles de la financiación: Howard Hughes Medical Institute | ||
| 536 | |a Detalles de la financiación: Fundación Antorchas | ||
| 536 | |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas | ||
| 536 | |a Detalles de la financiación: We want to acknowledge the support of Fundación Instituto Leloir; we thank Diego Ferreiro, Leonardo Alonso and Laura Zarebski for helpful discussion and technical assistance. We also thank Dr. Santiago Di Pietro for E2C peptide production. This work was supported by grants from Fundación Antorchas and the Consejo Nacional de Investigaciones Cientı́ficas y Técnicas (CONICET). M.L.C. is a recipient of a CONICET fellowship; J.M.C. holds a Carrillo-Oñativia fellowship. F.A.G. is recipient of a Carrillo-Oñativia fellowship from the Health Ministry of Argentina and is a Howard Hughes Medical Institute International Research Scholar (2002–2006). | ||
| 593 | |a F. I. L. IIBBA-CONICET, IIB-FCEN-UBA, Av. Patricias Argentinas 435, Buenos Aires 1405, Argentina | ||
| 690 | 1 | 0 | |a ADJUVANT |
| 690 | 1 | 0 | |a ANTIBODY |
| 690 | 1 | 0 | |a ANTIGEN BINDING |
| 690 | 1 | 0 | |a CONFORMATIONAL EPITOPE |
| 690 | 1 | 0 | |a TRANSCRIPTION FACTOR |
| 690 | 1 | 0 | |a ADJUVANT |
| 690 | 1 | 0 | |a EPITOPE |
| 690 | 1 | 0 | |a MONOCLONAL ANTIBODY |
| 690 | 1 | 0 | |a TRANSCRIPTION FACTOR |
| 690 | 1 | 0 | |a TRANSCRIPTION FACTOR E2 |
| 690 | 1 | 0 | |a UNCLASSIFIED DRUG |
| 690 | 1 | 0 | |a ANTIBODY RESPONSE |
| 690 | 1 | 0 | |a ANTIBODY SPECIFICITY |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a DNA BINDING |
| 690 | 1 | 0 | |a DNA DETERMINATION |
| 690 | 1 | 0 | |a DNA SEQUENCE |
| 690 | 1 | 0 | |a EMULSION |
| 690 | 1 | 0 | |a ENZYME REGULATION |
| 690 | 1 | 0 | |a ENZYME STABILITY |
| 690 | 1 | 0 | |a EPITOPE MAPPING |
| 690 | 1 | 0 | |a HUMORAL IMMUNITY |
| 690 | 1 | 0 | |a IMMUNIZATION |
| 690 | 1 | 0 | |a MACROMOLECULE |
| 690 | 1 | 0 | |a MOLECULAR RECOGNITION |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a PAPILLOMA VIRUS |
| 690 | 1 | 0 | |a PRIORITY JOURNAL |
| 690 | 1 | 0 | |a PROTEIN CONFORMATION |
| 690 | 1 | 0 | |a PROTEIN INTERACTION |
| 690 | 1 | 0 | |a TRANSCRIPTION INITIATION |
| 690 | 1 | 0 | |a TRANSCRIPTION REGULATION |
| 690 | 1 | 0 | |a VIRUS EXPRESSION |
| 690 | 1 | 0 | |a VIRUS TRANSCRIPTION |
| 690 | 1 | 0 | |a HUMAN PAPILLOMAVIRUS |
| 690 | 1 | 0 | |a PAPILLOMAVIRUS |
| 700 | 1 | |a Centeno, J.M. | |
| 700 | 1 | |a De Prat-Gay, G. | |
| 700 | 1 | |a Goldbaum, F.A. | |
| 773 | 0 | |d Elsevier, 2003 |g v. 534 |h pp. 202-206 |k n. 1-3 |p FEBS Lett. |x 00145793 |w (AR-BaUEN)CENRE-552 |t FEBS Letters | |
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