The mechanisms of brush border Na+/H+ exchanger activation by corticosteroids
Background: Previously we showed that corticosterone and aldosterone increased proton fluxes in proximal tubule, by micropuncture and stationary microperfusion. Since the Na+/H+ exchanger is responsible for the main proximal proton secretion, we have now evaluated the effects aldosterone on Na+/H+ e...
Guardado en:
| Autor principal: | |
|---|---|
| Otros Autores: | , , , |
| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
2003
|
| Acceso en línea: | Registro en Scopus Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| LEADER | 10465caa a22011537a 4500 | ||
|---|---|---|---|
| 001 | PAPER-5059 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203441.0 | ||
| 008 | 190411s2003 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0037297084 | |
| 024 | 7 | |2 cas |a acridine orange, 494-38-2, 65-61-2; aldosterone, 52-39-1, 6251-69-0; corticosterone, 50-22-6; gluconate sodium, 527-07-1; sodium proton exchange protein, 204597-32-0; Aldosterone, 52-39-1; Anti-Inflammatory Agents; Corticosterone, 50-22-6; Gluconates; gluconic acid, 526-95-4; Protein Isoforms; Slc9a2 protein, rat; Sodium-Hydrogen Antiporter; sodium-hydrogen exchanger 3 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a MSMOF | ||
| 100 | 1 | |a Zallocchi, M. | |
| 245 | 1 | 4 | |a The mechanisms of brush border Na+/H+ exchanger activation by corticosteroids |
| 260 | |c 2003 | ||
| 270 | 1 | 0 | |m Damasco, M.C.; Dep. Quimica Biologica, Pabellón 2, Ciudad Universitaria, 1428 Buenos Aires, Argentina; email: cdamasco@qb.fcen.uba.ar |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Biemesderfer, D., Pizzonia, J., Abu-AlFa, A., NHE3: A Na+/H+ exchanger isoform of the renal brush border (1993) Am J Physiol, 265, pp. 736-742 | ||
| 504 | |a Freiberg, J., Kinsella, J., Sacktor, B., Glucocorticoids increase the Na+-H+ exchange and decrease the Na+ gradient-dependent phosphate-uptake systems in renal brush border membrane vesicles (1982) Proc Natl Acad Sci USA, 79, pp. 4932-4936 | ||
| 504 | |a Igarreta, P., Calvo, J., Paladini, A., Damasco, M., Acute effects of two natural corticosteroids on the kinetics of the Na+/H+ exchanger in rat renal brush-border membranes (1997) Can J Physiol Pharmacol, 75, pp. 1226-1231 | ||
| 504 | |a Kinsella, J., Freiberg, J., Sacktor, B., Glucocorticoid activation of Na+/H+ exchange in renal brush border vesicles: Kinetic effects (1985) Am J Physiol, 27, pp. 233-239 | ||
| 504 | |a Damasco, M., Malnic, G., Effects of corticosteroids on proximal tubular acidification in the rat (1987) Miner Electrolyte Metab, 13, pp. 26-32 | ||
| 504 | |a Doucet, A., Katz, A., Mineralocorticoid receptors along the nephron: [3H]aldosterone binding in rat tubules (1981) Am J Physiol, 241, pp. 605-611 | ||
| 504 | |a Farman, N., Bonvalet, J., Aldosterone binding in isolated tubules. III. Autoradiography along the rat nepron (1983) Am J Physiol, 245, pp. 606-614 | ||
| 504 | |a Vandewalle, A., Farman, N., Bencsath, P., Bonvalet, J., Aldosterone binding along the rabbit nephron: An autoradiographic study on isolated tubules (1981) Am J Physiol, 240, pp. 172-179 | ||
| 504 | |a Hulter, H., Licht, J., Glynn, R., Sebastian, A., Renal acidosis in mineralocorticoid deficiency is not dependent on NaCl depletion or hyperkalemia (1979) Am J Physiol, 236, pp. 283-294 | ||
| 504 | |a Zimmermann, E., Smyrl, R., Crutchlow, V., Suppression of pituitary-adrenal response to stress with physiological plasma levels of corticosterone in the female rat (1972) Neuroendocrinology, 10, pp. 246-256 | ||
| 504 | |a Damasco, M., Vallverdú, R., Ceńal, J., Effects of 18-hydroxycorticosterone and of aldosterone on acid-base parameters in the arterial blood of adrenalectomized rats (1983) Acta Physiol Latinoam, 33, pp. 283-292 | ||
| 504 | |a Dubrovsky, A., Nair, R., Byers, M., Levine, D., Renal net acid excretion in the adrenalectomized rat (1981) Kidney Int, 19, pp. 516-528 | ||
| 504 | |a Lowry, O., Rosebrough, N., Farr, A., Randall, R., Protein measurement with the Folin phenol reagent (1951) J Biol Chem, 193, pp. 265-275 | ||
| 504 | |a Pecci, A., Cozza, E., Devlin, M., Endothelin-1 stimulation of aldosterone and zona glomerulosa ouabain-sensitive sodium/potassium-ATPase (1994) J Steroid Biochem Mol Biol, 50, pp. 49-53 | ||
| 504 | |a Warnock, D., Reenstra, W., Yee, V., Na+/H+ antiporter of brush border vesicles: Studies with acridine orange uptake (1982) Am J Physiol, 242, pp. 733-739 | ||
| 504 | |a Baum, M., Moe, O., Gentry, D., Alpern, R., Effect of glucocorticoids on renal cortical NHE-3 and NHE-1 mRNA (1994) Am J Physiol (Renal, Fluid and Electrolyte Physiology), 267, pp. 437-442 | ||
| 504 | |a Yip, K., Tse, C., McDonough, A., Marsh, D., Redistribution of Na+/H+ exchanger isoform NHE3 in proximal tubules induced by acute and chronic hypertension (1998) Am J Physiol, 275, pp. 565-575 | ||
| 504 | |a Otsu, K., Kinsella, J., Heller, P., Froehlich, J., Dependence of the Na+/H+ exchange in the pre-steady state (1993) J Biol Chem, 268, pp. 3184-3193 | ||
| 504 | |a Gupta, N., Tarif, S., Seikaly, M., Baum, M., Role of glucocorticoids in the maturation of the rat renal Na+/H+ antiporter (NHE3) (2001) Kidney Int, 60, pp. 173-181 | ||
| 504 | |a Chow, C., Regulation and intracellular localization of the epithelial isoform of the Na+/H+ exchangers NHE2 and NHE3 (1999) Clin Invest Med, 22, pp. 195-206 | ||
| 504 | |a Wiederholt, M., Stolte, H., Brecht, J., Hierholzer, K., Mikropunktions-untersuchungen über den einfluss von aldosteron, cortison und dexamethason auf die renale natriumresorption adrenalektomierter ratten (1966) Pflügers Archiv, 292, pp. 316-333 | ||
| 504 | |a Hierholzer, K., Stolte, H., The proximal and distal tubular action of adrenal steroids on Na+ reabsorption (1969) Nephron, 6, pp. 188-204 | ||
| 504 | |a Malnic, G., Ansaldo, M., Lantos, C., Damasco, M., Regulation of nephron acidification by corticosteroids (1997) Braz J Med Biol Res, 30, pp. 479-486 | ||
| 504 | |a Donowitz, M., De La Horra, C., Calonge, M., In birds, NHE2 is major brush-border Na+/H+ exchanger in colon and is increased by low-NaCl diet (1998) Am J Physiol, 274, pp. 1659-1669 | ||
| 504 | |a Wehling, M., Käsmayr, J., Theisen, K., Rapid effects of mineralocorticoids on sodium-proton exchanger: Genomic or nongenomic pathway? (1991) Am J Physiol, 260, pp. 719-726 | ||
| 504 | |a Wehling, M., Bauer, M., Ulsenheimer, A., Nongenomic effects of aldosterone on intracellular pH in vascular smooth muscle cells (1996) Biochem Biophys Res Commun, 223, pp. 181-186 | ||
| 504 | |a Schneider, M., Ulsenheimer, A., Christ, M., Wehling, M., Nongenomic effects of aldosterone on intracellular calcium in porcine endothelial cells (1997) Am J Physiol, 272, pp. 616-620 | ||
| 504 | |a Gekle, M., Silbernagl, S., Wunsch, S., Non-genomic action of the mineralocorticoid aldosterone on cytosolic sodium in cultured kidney cells (1998) J Physiol (Lond), 511, pp. 255-263 | ||
| 504 | |a Winter, D., Schneider, M., O'Sullivan, G., Rapid effects of aldosterone on sodium-hydrogen exchange in isolated colonic crypts (1999) J Membr Biol, 170, pp. 17-26 | ||
| 520 | 3 | |a Background: Previously we showed that corticosterone and aldosterone increased proton fluxes in proximal tubule, by micropuncture and stationary microperfusion. Since the Na+/H+ exchanger is responsible for the main proximal proton secretion, we have now evaluated the effects aldosterone on Na+/H+ exchange activity in brush border vesicles. In order to evaluate the mechanism of action of glucocorticoids and mineralocorticoids, we studied the comparative effects of corticosterone and aldosterone on the abundance of NHE3 and NHE2 isoforms. Material/Methods: We isolated renal brush border vesicles from rats by differential centrifugation in sham-operated, adrenalectomized, and adrenalectomized-aldosterone treated (ADX + aldosterone) animals. We measured the kinetics of H+ transport in response to increasing concentrations of Sodium Gluconate by fluorimetry using acridine orange. For Na+/H+ exchanger abundance we used Western blot analysis of brush border proteins in the above groups and in adrenalectomized-corticosterone treated rats. Results: The Vmax in adrenalectomized animals was 22,162±1828 fluorescence units/min; in sham animals, 37,020±2722; and in ADX + aldosterone, 42,344±3044 (p<0.01 adrenalectomized vs others). No differences in Km were observed. Adrenalectomy decreased NHE3 abundance over Sham by 32% without modifying NHE2. Corticosterone-replacement enhanced NHE3 abundance by 76% and failed to increase NHE2. Aldosterone enhanced NHE2 abundance by 75% and did not increase NHE3. Conclusion: Mineralocorticoids enhance Na+/H+ exchange activity by increasing NHE2 abundance; glucocorticoids, by increasing NHE3 abundance. |l eng | |
| 593 | |a Department of Biochemistry, FCEN, U.B.A, Buenos Aires, Argentina | ||
| 593 | |a Instituto de Ciencias Biomedicas, USP, Sao Paulo, Brazil | ||
| 593 | |a CONICET, Buenos Aires, Argentina | ||
| 593 | |a Dep. Quimica Biologica, Pabellón 2, Ciudad Universitaria, 1428 Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a ALDOSTERONE |
| 690 | 1 | 0 | |a GLUCOCORTICOIDS |
| 690 | 1 | 0 | |a H+ TRANSPORT |
| 690 | 1 | 0 | |a NHE2 |
| 690 | 1 | 0 | |a NHE3 |
| 690 | 1 | 0 | |a ACRIDINE ORANGE |
| 690 | 1 | 0 | |a ALDOSTERONE |
| 690 | 1 | 0 | |a CORTICOSTEROID |
| 690 | 1 | 0 | |a CORTICOSTERONE |
| 690 | 1 | 0 | |a GLUCONATE SODIUM |
| 690 | 1 | 0 | |a MINERALOCORTICOID |
| 690 | 1 | 0 | |a SODIUM PROTON EXCHANGE PROTEIN |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a BRUSH BORDER |
| 690 | 1 | 0 | |a FLUOROMETRY |
| 690 | 1 | 0 | |a KIDNEY PROXIMAL TUBULE |
| 690 | 1 | 0 | |a MOLECULAR DYNAMICS |
| 690 | 1 | 0 | |a MOLECULAR MECHANICS |
| 690 | 1 | 0 | |a PROTON TRANSPORT |
| 690 | 1 | 0 | |a RECEPTOR AFFINITY |
| 690 | 1 | 0 | |a TRANSPORT KINETICS |
| 690 | 1 | 0 | |a UPREGULATION |
| 690 | 1 | 0 | |a WESTERN BLOTTING |
| 690 | 1 | 0 | |a ADRENALECTOMY |
| 690 | 1 | 0 | |a ALDOSTERONE |
| 690 | 1 | 0 | |a ANIMALS |
| 690 | 1 | 0 | |a ANTI-INFLAMMATORY AGENTS |
| 690 | 1 | 0 | |a CORTICOSTERONE |
| 690 | 1 | 0 | |a GLUCONATES |
| 690 | 1 | 0 | |a KIDNEY TUBULES, PROXIMAL |
| 690 | 1 | 0 | |a MALE |
| 690 | 1 | 0 | |a MICROVILLI |
| 690 | 1 | 0 | |a PROTEIN ISOFORMS |
| 690 | 1 | 0 | |a RATS |
| 690 | 1 | 0 | |a RATS, SPRAGUE-DAWLEY |
| 690 | 1 | 0 | |a SODIUM-HYDROGEN ANTIPORTER |
| 700 | 1 | |a Igarreta, P. | |
| 700 | 1 | |a Calvo, J.C. | |
| 700 | 1 | |a Reboucas, N.A. | |
| 700 | 1 | |a Damasco, M.C. | |
| 773 | 0 | |d 2003 |g v. 9 |h pp. BR85-BR90 |k n. 2 |p Med. Sci. Monit. |x 12341010 |t Medical Science Monitor | |
| 856 | 4 | 1 | |u https://www.scopus.com/inward/record.uri?eid=2-s2.0-0037297084&partnerID=40&md5=a4092e0be5b595a2144884a0a50c3f2d |y Registro en Scopus |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_12341010_v9_n2_pBR85_Zallocchi |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_12341010_v9_n2_pBR85_Zallocchi |y Registro en la Biblioteca Digital |
| 961 | |a paper_12341010_v9_n2_pBR85_Zallocchi |b paper |c PE | ||
| 962 | |a info:eu-repo/semantics/article |a info:ar-repo/semantics/artículo |b info:eu-repo/semantics/publishedVersion | ||
| 999 | |c 66012 | ||