Trypanosoma cruzi TcSRPK, the first protozoan member of the SRPK family, is biochemically and functionally conserved with metazoan SR protein-specific kinases
A novel SR protein-specific kinase (SRPK) from the SRPK family was identified for the first time in a protozoan organism. The primary structure of the protein, named TcSRPK, presents a significant degree of identity with other metazoan members of the family. In vitro phosphorylation experiments show...
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| Otros Autores: | , , , , , , , , , , |
| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
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2003
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
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| LEADER | 18719caa a22021257a 4500 | ||
|---|---|---|---|
| 001 | PAPER-5028 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203438.0 | ||
| 008 | 190411s2003 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-12244260116 | |
| 024 | 7 | |2 Molecular Sequence Numbers |a GENBANK: AY101382, U09564; | |
| 024 | 7 | |2 cas |a fibronectin, 86088-83-7; protein kinase, 9026-43-1; RNA, 63231-63-0; Antibodies, Monoclonal; Globins, 9004-22-2; Protein-Serine-Threonine Kinases, EC 2.7.1.37 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a MBIPD | ||
| 100 | 1 | |a Portal, D. | |
| 245 | 1 | 0 | |a Trypanosoma cruzi TcSRPK, the first protozoan member of the SRPK family, is biochemically and functionally conserved with metazoan SR protein-specific kinases |
| 260 | |c 2003 | ||
| 270 | 1 | 0 | |m Torres, H.N.; Fac. de Ciencias Exactas y Naturales, Inst. Invest. Ingenieria Genet. B., Universidad de Buenos Aires, Buenos Aires, Argentina; email: torres@proteus.dna.uba.ar |
| 506 | |2 openaire |e Política editorial | ||
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| 504 | |a Ismaili, N., Pérez-Morga, D., Walsh, P., Mayeda, A., Pays, A., Tebabi, P., Characterization of a SR protein from Trypanosoma bucei with homology to RNA-binding cis-splicing proteins (1999) Mol. Biochem. Parasitol., 102, pp. 103-115 | ||
| 504 | |a Ismaili, N., Pérez-Morga, D., Walsh, P., Cadogan, M., Pays, A., Tebabi, P., Characterization of a Trypanosoma brucei SR domain-containing protein bearing homology to cis-spliceosomal U1 70 kDa proteins (2000) Mol. Biochem. Parasitol., 106, pp. 109-120 | ||
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| 504 | |a Koizumi, J., Okamoto, Y., Onogi, H., Mayeda, A., Krainer, A.R., Hagiwara, M., The subcellular localization of SF2/ASF is regulated by direct interaction with SR protein kinases (SRPKs) (1999) J. Biol. Chem., 274, pp. 11125-11131 | ||
| 520 | 3 | |a A novel SR protein-specific kinase (SRPK) from the SRPK family was identified for the first time in a protozoan organism. The primary structure of the protein, named TcSRPK, presents a significant degree of identity with other metazoan members of the family. In vitro phosphorylation experiments showed that TcSRPK has the same substrate specificity relative to other SRPKs. TcSRPK was able to generate a mAb104-recognized phosphoepitope, a SRPK landmark. Expression of TcSRPK in different Schizosaccharomyces pombe strains lead to conserved phenotypes, indicating that TcSRPK is a functional homologue of metazoan SRPKs. In functional alternative splicing assays in vivo in HeLa cells, TcSRPK enhanced SR protein-dependent inclusion of the EDI exon of the fibronectin minigene. When tested in vitro, it inhibited splicing either on nuclear extracts or on splicing-deficient S100 extracts complemented with ASF/SF2. This inhibition was similar to that observed with human SRPK1. This work constitutes the first report of a member of this family of proteins and the existence of an SR-network in a protozoan organism. The implications in the origins and control of splicing are discussed. © 2002 Elsevier Science B.V. All rights reserved. |l eng | |
| 536 | |a Detalles de la financiación: Agencia Nacional de Promoción Científica y Tecnológica | ||
| 536 | |a Detalles de la financiación: Universidad de Buenos Aires | ||
| 536 | |a Detalles de la financiación: Ministerio de Salud de la Nación, MSAL | ||
| 536 | |a Detalles de la financiación: Howard Hughes Medical Institute | ||
| 536 | |a Detalles de la financiación: Fundación Antorchas | ||
| 536 | |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas | ||
| 536 | |a Detalles de la financiación: This study was supported in part by the Consejo Nacional de Investigaciones Cientı́ficas y Técnicas (Argentina), the University of Buenos Aires (Argentina), the Agencia Nacional de Promoción Cientı́fica y Tecnológica (Argentina), the Carrillo-Oñativia Fellowship, Ministerio de Salud (Argentina), the Fundación Antorchas (Argentina), the International Centre for Genetic Engineering and Biotechnology (Trieste, Italy), WHO-TDR, and the Howard Hughes Medical Institute (USA). We thank Dr. Kevin Ryan from Dr. Manley’s laboratory for critical reading of the manuscript and important help in the splicing reactions, and Kurt Fried of City of Hope for editing the manuscript. D.P., G.S.L., and S.K., are fellows of the University of Buenos Aires (UBA), and C.A.P. is a fellow of the Consejo Nacional de Investigaciones Cientı́ficas y Técnicas (CONICET). | ||
| 593 | |a Fac. de Ciencias Exactas y Naturales, Inst. Invest. Ingenieria Genet. B., Universidad de Buenos Aires, Buenos Aires, Argentina | ||
| 593 | |a Lab. de Fisiol. y Biol. Molecular, Fac. de Ciencias Exactas y Naturales, Buenos Aires, Argentina | ||
| 593 | |a Department of Biological Sciences, Columbia University, New York, NY 10027, United States | ||
| 593 | |a Division of Molecular Biology, Beckman Res. Inst. the City of Hope, Duarte, CA, United States | ||
| 690 | 1 | 0 | |a SR PROTEIN KINASE |
| 690 | 1 | 0 | |a SR-NETWORK |
| 690 | 1 | 0 | |a SRPK |
| 690 | 1 | 0 | |a TRANS-SPLICING |
| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 690 | 1 | 0 | |a CELL EXTRACT |
| 690 | 1 | 0 | |a EPITOPE |
| 690 | 1 | 0 | |a FIBRONECTIN |
| 690 | 1 | 0 | |a ISOENZYME |
| 690 | 1 | 0 | |a MONOCLONAL ANTIBODY |
| 690 | 1 | 0 | |a MONOCLONAL ANTIBODY 104 |
| 690 | 1 | 0 | |a PROTEIN KINASE |
| 690 | 1 | 0 | |a PROTEIN S 100 |
| 690 | 1 | 0 | |a PROTOZOAL PROTEIN |
| 690 | 1 | 0 | |a RIBONUCLEOPROTEIN |
| 690 | 1 | 0 | |a RNA |
| 690 | 1 | 0 | |a SERINE ARGININE PROTEIN |
| 690 | 1 | 0 | |a SERINE ARGININE PROTEIN KINASE |
| 690 | 1 | 0 | |a SPLICING FACTOR 2 |
| 690 | 1 | 0 | |a UNCLASSIFIED DRUG |
| 690 | 1 | 0 | |a ALTERNATIVE RNA SPLICING |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a CELL NUCLEUS |
| 690 | 1 | 0 | |a CONTROLLED STUDY |
| 690 | 1 | 0 | |a ENZYME PHOSPHORYLATION |
| 690 | 1 | 0 | |a ENZYME SPECIFICITY |
| 690 | 1 | 0 | |a EXON |
| 690 | 1 | 0 | |a FUNGAL STRAIN |
| 690 | 1 | 0 | |a GENE |
| 690 | 1 | 0 | |a HELA CELL |
| 690 | 1 | 0 | |a HUMAN |
| 690 | 1 | 0 | |a HUMAN CELL |
| 690 | 1 | 0 | |a IN VITRO STUDY |
| 690 | 1 | 0 | |a IN VIVO STUDY |
| 690 | 1 | 0 | |a INHIBITION KINETICS |
| 690 | 1 | 0 | |a METAZOON |
| 690 | 1 | 0 | |a MOLECULAR RECOGNITION |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a NUCLEOTIDE SEQUENCE |
| 690 | 1 | 0 | |a PHENOTYPE |
| 690 | 1 | 0 | |a PRIORITY JOURNAL |
| 690 | 1 | 0 | |a PROTEIN EXPRESSION |
| 690 | 1 | 0 | |a PROTEIN FAMILY |
| 690 | 1 | 0 | |a PROTEIN FUNCTION |
| 690 | 1 | 0 | |a PROTEIN STRUCTURE |
| 690 | 1 | 0 | |a SCHIZOSACCHAROMYCES POMBE |
| 690 | 1 | 0 | |a SEQUENCE HOMOLOGY |
| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 690 | 1 | 0 | |a AMINO ACID SEQUENCE |
| 690 | 1 | 0 | |a ANIMALS |
| 690 | 1 | 0 | |a ANTIBODIES, MONOCLONAL |
| 690 | 1 | 0 | |a CLONING, MOLECULAR |
| 690 | 1 | 0 | |a GENES, PROTOZOAN |
| 690 | 1 | 0 | |a GLOBINS |
| 690 | 1 | 0 | |a HELA CELLS |
| 690 | 1 | 0 | |a HUMANS |
| 690 | 1 | 0 | |a MOLECULAR SEQUENCE DATA |
| 690 | 1 | 0 | |a PROTEIN STRUCTURE, TERTIARY |
| 690 | 1 | 0 | |a PROTEIN-SERINE-THREONINE KINASES |
| 690 | 1 | 0 | |a SCHIZOSACCHAROMYCES |
| 690 | 1 | 0 | |a SEQUENCE ALIGNMENT |
| 690 | 1 | 0 | |a SEQUENCE HOMOLOGY, AMINO ACID |
| 690 | 1 | 0 | |a SPECIES SPECIFICITY |
| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 690 | 1 | 0 | |a METAZOA |
| 690 | 1 | 0 | |a PROTOZOA |
| 690 | 1 | 0 | |a SCHIZOSACCHAROMYCES |
| 690 | 1 | 0 | |a SCHIZOSACCHAROMYCES POMBE |
| 690 | 1 | 0 | |a TRYPANOSOMA |
| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 700 | 1 | |a Lobo, G.S. | |
| 700 | 1 | |a Kadener, S. | |
| 700 | 1 | |a Prasad, J. | |
| 700 | 1 | |a Espinosa, J.M. | |
| 700 | 1 | |a Pereira, C.A. | |
| 700 | 1 | |a Tang, Z. | |
| 700 | 1 | |a Lin, R.-J. | |
| 700 | 1 | |a Manley, J.L. | |
| 700 | 1 | |a Kornblihtt, A.R. | |
| 700 | 1 | |a Flawiá, M.M. | |
| 700 | 1 | |a Torres, H.N. | |
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