ErbB2 and EGFR are downmodulated during the differentiation of 3T3-L1 preadipocytes

The expression of receptors belonging to the epidermal growth factor receptor subfamily has been largely studied these last years in epithelial cells mainly as involved in cell proliferation and malignant progression. Although much work has focused on the role of these growth factor receptors in the...

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Autor principal: Pagano, E.
Otros Autores: Calvo, J.C
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 2003
Acceso en línea:Registro en Scopus
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Registro en la Biblioteca Digital
Aporte de:Registro referencial: Solicitar el recurso aquí
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024 7 |2 scopus  |a 2-s2.0-0141988841 
024 7 |2 cas  |a dexamethasone, 50-02-2; growth hormone, 36992-73-1, 37267-05-3, 66419-50-9, 9002-72-6; Dexamethasone, 50-02-2; Epidermal Growth Factor, 62229-50-9; heregulin alpha; heregulin beta1, 155646-83-6; Insulin, 11061-68-0; Neuregulin-1; Receptor, Epidermal Growth Factor, EC 2.7.1.112; Receptor, erbB-2, EC 2.7.1.112 
040 |a Scopus  |b spa  |c AR-BaUEN  |d AR-BaUEN 
030 |a JCEBD 
100 1 |a Pagano, E. 
245 1 0 |a ErbB2 and EGFR are downmodulated during the differentiation of 3T3-L1 preadipocytes 
260 |c 2003 
270 1 0 |m Pagano, E.; Lab. de Quimica de Proteoglicanos, Inst. de Biol. y Med. Experimental, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina; email: epagano@dna.uba.ar 
506 |2 openaire  |e Política editorial 
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504 |a Hynes, N.E., Stern, D.F., The biology of erbB-2/HER-2 and its role in cancer (1994) Biochim Biophys Acta, 1198, pp. 165-184 
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504 |a Kim, S., Houtman, J.C.D., Jiang, J., Ruppert, M., Bertics, P.J., Frank, S.J., Growth hormone-induced alteration in erbB-2 phosphorylation status in 3T3-F442A fibroblasts (1999) J Biol Chem, 274, pp. 36015-36024 
504 |a Lee, H., Maihle, N., Isolation and characterization of four alternate c-erbB3 transcripts expressed in ovarian carcinoma-derived cell lines and normal human tissues (1998) Oncogene, 16, pp. 3243-3252 
504 |a Lim, H., Dey, S.K., Das, S.K., Differential expression of the erbB2 gene in the periimplantation mouse uterus: Potential mediator of signaling by epidermal growth factor-like growth factors (1997) Endocrinology, 138, pp. 1328-1337 
504 |a Markogiannakis, E., Georgoulias, V., Margioris, A., Zoumakis, E., Stournaras, C., Gravanis, A., Estrogens and glucocorticoids induce the expression of c-erbB2/NEU receptor in Ishikawa human endometrial cells (1997) Life Sci, 61, pp. 1083-1095 
504 |a Morrison, R.F., Farmer, S.R., Symposium: Adipocyte function, differentiation and metabolism. Hormonal signaling and transcriptional control of adipocyte differentiation (2000) J Nutr, 130, pp. 3116S-3121S 
504 |a Mulligan, C., Rochford, J., Denyer, G., Stephens, R., Yeo, G., Freeman, T., Siddle, K., O'Rahilly, S., Microarray analysis of insulin and insulin-growth factor-1 (IGF-1) receptor signaling reveals the selective up-regulation of the mitogen heparin-binding EGF-like growth factor by IGF-1 (2002) J Biol Chem, 277 (45), pp. 42480-42487 
504 |a Olayioye, M.A., Neve, R., Lane, H.A., Hynes, N.E., The ErbB signaling network: Receptor heterodimerization in development and cancer (2000) EMBO J, 19, pp. 3159-3167 
504 |a Peles, E., Bacus, S.S., Koski, R.A., Lu, H.S., Wen, D., Ogden, S.G., Levy, R.B., Yarden, Y., Isolation of the neu/HER-2 stimulatory ligand: A 44 kDa glycoprotein that induces differentiation of mammary tumor cells (1992) Cell, 69, pp. 205-216 
504 |a Press, M.F., Cordon-Cardo, C., Slamon, D.J., Expression of the HER-2/neu proto-oncogene in normal human adult and fetal tissues (1990) Oncogene, 5, pp. 953-962 
504 |a Reed, B.C., Kaufmann, S.H., Mackall, J.C., Student, A.K., Lane, M.D., Alterations in insulin binding accompaying differentiation of 3T3-L1 preadipocytes (1977) Proc Natl Acad Sci USA, 74, pp. 4876-4880 
504 |a Roudabush, F.L., Pierce, K.L., Maudsley, S., Khan, K.D., Lutrell, L.M., Tansactivation of the EGF receptor mediates IGF-1-stimulated Shc phosphorylation and ERK1/2 activation in COS-7 cells (2000) J Biol Chem, 275, pp. 22583-22589 
504 |a Schmidt, W., Pöll-Jordan, G., Löffler, G., Adipose convertion of 3T3-L1 cells in a serum-free culture system depends on epidermal growth factor, insulin-like growth factor-1, corticosterone, and cyclic AMP (1990) J Biol Chem, 265, pp. 15489-15495 
504 |a Schroeder, J.A., Lee, D.C., Dynamic expression and activation of ERBB receptors in the developing mouse mammary gland (1998) Cell Growth Differ, 9, pp. 451-464 
504 |a Serrero, G., EGF inhibits the differentiation of adipocyte precursors in primary cultures (1987) Biochem Biophys Res Commun, 146, pp. 194-202 
504 |a Serrero, G., Mills, D., Physiologic role of EGF on adipose tissue development in vivo (1991) Proc Natl Acad Sci USA, 88, pp. 3912-3916 
504 |a Sliwkowski, M.X., Schaefer, G., Akita, R.W., Lofgren, J.A., Fitzpatrick, V.D., Nuijens, A., Fendly, B.M., Carraway K.L. III, Coexpression of erbB2 and erbB3 proteins reconstitutes a high affinity receptor for heregulin (1994) J Biol Chem, 269, pp. 14661-14665 
504 |a Smith, P.J., Wise, L.S., Berkowitz, R., Wan, C., Rubin, C.S., Insulin-like growth factor-1 is an essential regulator of the differentiation of 3T3-L1 adipocytes (1988) J Biol Chem, 263, pp. 9402-9408 
504 |a Srivinisan, R., Leverton, K., Sheldon, H., Hurst, H., Sarraf, C., Gullick, W., Intracellular expression of the truncated extracellular domain of c-erbB3/HER3 (2001) Cell Signal, 13, pp. 321-330 
504 |a Stern, D.F., Kamps, M.P., EGF-stimulated tyrosine phosphorylation of p185neu: A potential model for receptor interactions (1988) EMBO J, 7, pp. 995-1001 
504 |a Yao, H., Labudda, K., Rim, C., Loda, M., Stork, P.J., Cyclic adenosine monophosphate can convert epidermal growth factor into a differentiating factor in neuronal cells (1995) J Biol Chem, 270, pp. 20748-20753 
504 |a Yao, H., York, R.D., Misra-Press, A., Carr, D.W., Stork, P.J., The cyclic adenosine monophosphate-dependent protein kinase (PKA) is required for the sustained activation of mitogen-activated kinases and gene expression by nerve growth factor (1998) J Biol Chem, 273, pp. 8240-8247 
504 |a Zizola, C., Balaña, M.E., Sandoval, M., Calvo, J.C., Changes in IGF-1 receptor and IGF-1 mRNA during differentiation of 3T3-L1 preadipocytes (2002) Biochimie, 84, pp. 975-980 
520 3 |a The expression of receptors belonging to the epidermal growth factor receptor subfamily has been largely studied these last years in epithelial cells mainly as involved in cell proliferation and malignant progression. Although much work has focused on the role of these growth factor receptors in the differentiation of a variety of tissues, there is little information in regards to normal stromal cells. We investigated erbB2 expression in the murine fibroblast cell line Swiss 3T3L1, which naturally or hormonally induced undergoes adipocyte differentiation. We found that the Swiss 3T3-L1 fibroblasts express erbB2, in addition to EGFR, and in a quantity comparable to or even greater than the breast cancer cell line T47D. Proliferating cells increased erbB2 and EGFR levels when reaching confluence up to 4- and 10-fold, respectively. This expression showed a significant decrease when growth-arrested cells were stimulated to differentiate with dexamethasone and isobutyl-methylxanthine. Differentiated cells presented a decreased expression of both erbB2 and EGFR regardless of whether the cells were hormonally or spontaneously differentiated. EGF stimulation of serum-starved cells increased erbB2 tyrosine phosphorylation and retarded erbB2 migration in SDS-PAGE, suggesting receptor association and activation. Heregulin-α1 and β1, two EGF related factors, had no effect on erbB2 or EGFR phosphorylation. Although 3T3-L1 cells expressed heregulin, its specific receptors, erbB3 and erbB4, were not found. This is the first time in which erbB2 is reported to be expressed in an adipocytic cell line which does not depend on non EGF family growth factors (thyroid hormone, growth hormone, etc.) to accomplish adipose differentiation. Since erbB2 and EGFR expression were downmodulated as differentiation progressed it is conceivable that a mechanism of switching from a mitogenic to a differentiating signaling pathway may be involved, through regulation of the expression of these growth factor receptors. © 2003 Wiley-Liss, Inc.  |l eng 
593 |a Lab. de Quimica de Proteoglicanos, Inst. de Biol. y Med. Experimental, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina 
593 |a Universidad de Buenos Aires, Fac. de Ciencias Exactas y Naturales, Buenos Aires, Argentina 
690 1 0 |a 3T3-L1 CELLS 
690 1 0 |a ADIPOCYTE DIFFERENTIATION 
690 1 0 |a CAMP 
690 1 0 |a DEXAMETHASONE 
690 1 0 |a EGF 
690 1 0 |a EGFR 
690 1 0 |a ERBB2 
690 1 0 |a FIBROBLASTS 
690 1 0 |a HEREGULIN 
690 1 0 |a INSULIN 
690 1 0 |a TYROSINE PHOSPHORYLATION 
690 1 0 |a DEXAMETHASONE 
690 1 0 |a EPIDERMAL GROWTH FACTOR RECEPTOR 
690 1 0 |a GROWTH FACTOR RECEPTOR 
690 1 0 |a GROWTH HORMONE 
690 1 0 |a HEREGULIN ALPHA1 
690 1 0 |a HEREGULIN BETA1 
690 1 0 |a METHYLXANTHINE DERIVATIVE 
690 1 0 |a NEU DIFFERENTIATION FACTOR 
690 1 0 |a THYROID HORMONE 
690 1 0 |a UNCLASSIFIED DRUG 
690 1 0 |a ADIPOCYTE 
690 1 0 |a ANIMAL CELL 
690 1 0 |a ARTICLE 
690 1 0 |a CELL DIFFERENTIATION 
690 1 0 |a CELL MIGRATION 
690 1 0 |a CELL PROLIFERATION 
690 1 0 |a CONTROLLED STUDY 
690 1 0 |a EPITHELIUM CELL 
690 1 0 |a MALIGNANT TRANSFORMATION 
690 1 0 |a MITOGENESIS 
690 1 0 |a NONHUMAN 
690 1 0 |a ONCOGENE NEU 
690 1 0 |a POLYACRYLAMIDE GEL ELECTROPHORESIS 
690 1 0 |a PRIORITY JOURNAL 
690 1 0 |a PROADIPOCYTE 
690 1 0 |a PROTEIN EXPRESSION 
690 1 0 |a PROTEIN PHOSPHORYLATION 
690 1 0 |a 3T3-L1 CELLS 
690 1 0 |a ADIPOCYTES 
690 1 0 |a ANIMALS 
690 1 0 |a CELL DIFFERENTIATION 
690 1 0 |a DEXAMETHASONE 
690 1 0 |a EPIDERMAL GROWTH FACTOR 
690 1 0 |a HUMANS 
690 1 0 |a INSULIN 
690 1 0 |a MICE 
690 1 0 |a NEUREGULIN-1 
690 1 0 |a PHOSPHORYLATION 
690 1 0 |a RECEPTOR, EPIDERMAL GROWTH FACTOR 
690 1 0 |a RECEPTOR, ERBB-2 
690 1 0 |a TUMOR CELLS, CULTURED 
690 1 0 |a MURINAE 
700 1 |a Calvo, J.C. 
773 0 |d 2003  |g v. 90  |h pp. 561-572  |k n. 3  |p J. Cell. Biochem.  |x 07302312  |t Journal of Cellular Biochemistry 
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