DNA immunization with the ribosomal P2β gene of Trypanosoma cruzi fails to induce pathogenic antibodies
Patients with chronic Chagas' heart disease (cChHD) develop a strong IgG response against the C-terminal region of the Trypanosoma cruzi ribosomal P2β protein (TcP2β). These antibodies have been shown to exert an in vitro chronotropic effect on cardiocytes through stimulation of the β1-adrenerg...
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Elsevier Masson SAS
2003
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| LEADER | 15468caa a22013457a 4500 | ||
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| 001 | PAPER-4754 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203420.0 | ||
| 008 | 190411s2003 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0345528054 | |
| 024 | 7 | |2 cas |a cyclic AMP, 60-92-4 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a MCINF | ||
| 100 | 1 | |a Levitus, G. | |
| 245 | 1 | 0 | |a DNA immunization with the ribosomal P2β gene of Trypanosoma cruzi fails to induce pathogenic antibodies |
| 260 | |b Elsevier Masson SAS |c 2003 | ||
| 270 | 1 | 0 | |m Levin, M.J.; Lab. Biol. Molec. Enfermedad Chagas, Inst. Invest. Ing. Genet. Biol. M., Vuelta de Obligado 2490, Buenos Aires, 1428, Argentina; email: mlevin@dna.uba.ar |
| 506 | |2 openaire |e Política editorial | ||
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| 504 | |a Mesri, E., Levitus, G., Hontebeyrie-Joskowicz, M., Dighiero, G., Van Regenmortel, M.H.V., Levin, M.J., Major Trypanosoma cruzi determinant in Chagas' heart disease shares homology with the systemic lupus erythematosus ribosomal P protein epitope (1990) J. Clin. Microbiol., 28, pp. 1219-1224 | ||
| 504 | |a Aznar, C., López Bergami, P., Brandariz, S., Mariette, C., Liegeard, P., De Deus Alves, M.C., Luna Barreiro, E., Hontebeyrie-Joskowicz, M., Prevalence of anti-R13 antibodies in human Trypanosoma cruzi infection (1995) FEMS Immunol. Med. Microbiol., 12, pp. 231-238 | ||
| 504 | |a López Bergami, P., Cabeza Meckert, P., Kaplan, D., Levitus, G., Elias, F., Quintana, F., Van Regenmortel, M.H.V., Levin, M.J., Immunization with recombinant Trypanosoma cruzi ribosomal P2β protein induces changes in the electrocardiogram of immunized mice (1997) FEMS Immunol. Med. Microbiol., 18, pp. 75-85 | ||
| 504 | |a Sepulveda, P., Liegeard, P., Wallukat, G., Levin, M.J., Hontebeyrie, M., Modulation of cardiocyte functional activity by antibodies against Trypanosoma cruzi ribosomal P2 protein C-terminus (2000) Infect. Immun., 68, pp. 5114-5119 | ||
| 504 | |a Gurunathan, S., Wu, C.Y., Freidag, B.L., Seder, R.A., DNA vaccines: A key for inducing long-term cellular immunity (2000) Curr. Opin. Immunol., 12, pp. 442-447 | ||
| 504 | |a Mahler, E., Sepulveda, P., Jeannequin, O., Liegeard, P., Gounon, P., Wallukat, G., Eftekhari, P., Hontebeyrie, M., A monoclonal antibody against the immunodominant epitope of the ribosomal P2β protein of Trypanosoma cruzi interacts with the human β1-adrenergic receptor (2001) Eur. J. Immunol., 31, pp. 2210-2216 | ||
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| 504 | |a Schijman, A., Dusetti, N.J., Vázquez, M.P., Lafon, S., Levy-Yeyati, P., Levin, M.J., Nucleotide cDNA and complete deduced amino acid sequence of a Trypanosoma cruzi ribosomal P protein (P-JL5) (1990) Nucleic Acids Res., 18, p. 3399 | ||
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| 504 | |a Weiss, W.R., Ishii, K.J., Hedstrom, R.C., Sedegah, M., Ichino, M., Barnhart, K., Klinman, D.M., Hoffman, S.L., A plasmid encoding murine granulocyte-macrophage colony-stimulating factor increases protection conferred by a malaria DNA vaccine (1998) J. Immunol., 161, pp. 2325-2332 | ||
| 504 | |a Leclerc, C., Deriaud, E., Rojas, M., Whalen, R.G., The preferential induction of a Th1 immune response by DNA-based immunization is mediated by the immunostimulatory effect of plasmid DNA (1997) Cell Immunol., 179, pp. 97-106 | ||
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| 504 | |a Sharma, A.K., Khuller, G.K., DNA vaccines: Future strategies and relevance to intracellular pathogens (2001) Immunol. Cell Biol., 79, pp. 537-546 | ||
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| 504 | |a Garcia, F., Sepulveda, P., Liegeard, P., Gregoire, J., Hermann, E., Lemonnier, F., Langlade-Demoyen, P., Lone, Y.C., Identification of HLA-A*0201-restricted cytotoxic T-cell epitopes of Trypanosoma cruzi TcP2β protein in HLA-transgenic mice and patients (2003) Microbes Infect., 5, pp. 351-359 | ||
| 520 | 3 | |a Patients with chronic Chagas' heart disease (cChHD) develop a strong IgG response against the C-terminal region of the Trypanosoma cruzi ribosomal P2β protein (TcP2β). These antibodies have been shown to exert an in vitro chronotropic effect on cardiocytes through stimulation of the β1-adrenergic receptor (β1-AR). Moreover, the presence of antibodies recognizing the TcP2β C-terminus was associated with cardiac alterations in mice immunized with the corresponding recombinant protein. Here, we demonstrate that DNA immunization could be used to modulate the specificity of the anti-TcP2β humoral response in order to avoid the production of pathogenic antibodies. After DNA injection, we detected IgG antibodies that were directed only to internal epitopes of the TcP2β molecule and that did not exert anti-β1-AR functional activity, measured as an increase in intracellular cAMP levels of transfected COS-7 cells. Accordingly, DNA-immunized mice did not present electrocardiographic alterations. These data demonstrate that anti-TcP2β antibodies elicited by DNA immunization are completely different in their specificity and functional activity from those produced during T. cruzi infection. © 2003 Éditions scientifiques et médicales Elsevier SAS. All rights reserved. |l eng | |
| 536 | |a Detalles de la financiación: Howard Hughes Medical Institute | ||
| 536 | |a Detalles de la financiación: Universidad de Buenos Aires | ||
| 536 | |a Detalles de la financiación: Fondo para la Investigación Científica y Tecnológica, BID 1201/OC-AR 05-06802 | ||
| 536 | |a Detalles de la financiación: We are grateful to Evelina Mahler (INGEBI, Buenos Aires, Argentina) for helpful discussion. We also thank Dr. Mauricio Rodrigues (Escola Paulista de Medicina, São Paulo, Brazil) for kindly providing the pcDNA3/GM-CSF plasmid and Dr. Reza Mobini (Sahlgrenska University Hospital, Gothenburg, Sweden) for providing MAbM16. This work was supported by grants from (i) the World Health Organization/Special Program for Research and Training in Tropical Diseases, Universidad de Buenos Aires, (ii) FONCYT BID 1201/OC-AR 05-06802, and (iii) INSERM-CONICET Joint Research Program (Project Hontebeyrie/Levin). In addition, the work of Dr. Mariano J. Levin is partially supported by an International Research Scholar grant from the Howard Hughes Medical Institute, Chevy Chase, MD, USA. | ||
| 593 | |a Lab. Biol. Molec. Enfermedad Chagas, Inst. Invest. Ing. Genet. Biol. M., Vuelta de Obligado 2490, Buenos Aires, 1428, Argentina | ||
| 593 | |a Depto. Fisiol. y Biol. Molec. y Cel., Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina | ||
| 593 | |a U. Repliement Modelisation Proteines, Dept. de Biol. Structurale et Chimie, Institut Pasteur, 28, rue du Dr Roux, 75724 Paris Cedex 15, France | ||
| 690 | 1 | 0 | |a DNA IMMUNIZATION |
| 690 | 1 | 0 | |a RIBOSOMAL P2 PROTEIN |
| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 690 | 1 | 0 | |a CYCLIC AMP |
| 690 | 1 | 0 | |a DNA VACCINE |
| 690 | 1 | 0 | |a EPITOPE |
| 690 | 1 | 0 | |a IMMUNOGLOBULIN G ANTIBODY |
| 690 | 1 | 0 | |a PROTOZOAL PROTEIN |
| 690 | 1 | 0 | |a PROTOZOON ANTIBODY |
| 690 | 1 | 0 | |a RECOMBINANT PROTEIN |
| 690 | 1 | 0 | |a RIBOSOME PROTEIN |
| 690 | 1 | 0 | |a ANIMAL CELL |
| 690 | 1 | 0 | |a ANIMAL EXPERIMENT |
| 690 | 1 | 0 | |a ANIMAL MODEL |
| 690 | 1 | 0 | |a ANTIBODY DETECTION |
| 690 | 1 | 0 | |a ANTIBODY PRODUCTION |
| 690 | 1 | 0 | |a ANTIBODY SPECIFICITY |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a CELL STRAIN COS7 |
| 690 | 1 | 0 | |a CHAGAS DISEASE |
| 690 | 1 | 0 | |a CONTROLLED STUDY |
| 690 | 1 | 0 | |a DRUG EFFECT |
| 690 | 1 | 0 | |a ELECTROCARDIOGRAPHY |
| 690 | 1 | 0 | |a FEMALE |
| 690 | 1 | 0 | |a GENETIC TRANSFECTION |
| 690 | 1 | 0 | |a HUMAN |
| 690 | 1 | 0 | |a HUMORAL IMMUNITY |
| 690 | 1 | 0 | |a IMMUNIZATION |
| 690 | 1 | 0 | |a MOUSE |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a PRIORITY JOURNAL |
| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 690 | 1 | 0 | |a TRYPANOSOMIASIS |
| 690 | 1 | 0 | |a TRYPANOSOMA |
| 690 | 1 | 0 | |a TRYPANOSOMA CRUZI |
| 700 | 1 | |a Grippo, V. | |
| 700 | 1 | |a Labovsky, V. | |
| 700 | 1 | |a Ghio, S. | |
| 700 | 1 | |a Hontebeyrie, M. | |
| 700 | 1 | |a Levin, M.J. | |
| 773 | 0 | |d Elsevier Masson SAS, 2003 |g v. 5 |h pp. 1381-1388 |k n. 15 |p Microbes Infect. |x 12864579 |w (AR-BaUEN)CENRE-6100 |t Microbes and Infection | |
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