Hexachlorobenzene impairs glucose metabolism in a rat model of porphyria cutanea tarda: A mechanistic approach
Hexachlobenzene (HCB), one of the most persistent environmental pollutants, induces porphyria cutanea tarda (PCT). The aim of this work was to analyze the effect of HCB on some aspects of glucose metabolism, particularly those related to its neosynthesis in vivo. For this purpose, a time-course stud...
Guardado en:
| Autor principal: | |
|---|---|
| Otros Autores: | , , , , |
| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
2004
|
| Materias: | |
| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| LEADER | 18822caa a22016697a 4500 | ||
|---|---|---|---|
| 001 | PAPER-4702 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203416.0 | ||
| 008 | 190411s2004 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-1442352149 | |
| 024 | 7 | |2 cas |a glucose 6 phosphatase, 9001-39-2; glucose, 50-99-7, 84778-64-3; glycogen, 9005-79-2; hexachlorobenzene, 118-74-1, 55600-34-5; insulin, 9004-10-8; phosphoenolpyruvate carboxykinase (GTP), 9013-08-5; pyruvate carboxylase, 9014-19-1; pyruvate kinase, 9001-59-6; Enzyme Inhibitors; Enzymes; Fungicides, Industrial; Glucose, 50-99-7; Glucose-6-Phosphatase, EC 3.1.3.9; Hexachlorobenzene, 118-74-1; Phosphoenolpyruvate Carboxylase, EC 4.1.1.31; Pyruvate Carboxylase, EC 6.4.1.1; Pyruvate Kinase, EC 2.7.1.40 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a ARTOD | ||
| 100 | 1 | |a Mazzetti, M.B. | |
| 245 | 1 | 0 | |a Hexachlorobenzene impairs glucose metabolism in a rat model of porphyria cutanea tarda: A mechanistic approach |
| 260 | |c 2004 | ||
| 270 | 1 | 0 | |m De Viale, L.C.S.M.O'Higgins 4332, CP 1429, Buenos Aires, Argentina; email: smartin@qb.fcen.uba.ar |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Abdel-Wahab, Y.H.A., O'Harte, F.P.M., Barnett, C.R., Flatt, P.R., Glycation of insulin in the islet of Langerhans of normal and diabetic animals (1996) Diabetes, 45, pp. 1489-1496 | ||
| 504 | |a Baginski, E.S., Foa, P.P., Zak, B., Glucose-6-phosphatase (1974) Method of Enzymatic Analysis, 2nd Edn, 1, pp. 876-880. , Bermeyer HU (ed), Verlag Chemie/Academic Press, Weinheim, New York | ||
| 504 | |a Basabe, J.C., Karabatas, L.M., Arata, M., Pivetta, O.H., Cresto, J.C., Secretion and effect of somatostatin in early stages of the diabetic syndrome in C57BL/KsJ-mdb mice (1986) Diabetologia, 29, pp. 485-488 | ||
| 504 | |a Berndt, J., Messner, B., Still, J., Optical assay of pyruvate carboxylase in crude liver homogenate (1978) Anal Biochem, 86, pp. 154-158 | ||
| 504 | |a Billi De Catabbi, S., Sterin-Speziale, N., Fernandez, M.C., Minutolo, C., Aldonatti, C., San Martín De Viale, L., Time course hexachlorobenzene-induced alterations of lipid metabolism and their relation to porphyria (1997) Int J Biochem Cell Biol, 29, pp. 335-344 | ||
| 504 | |a Billi De Catabbi, S.C., Aldonatti, C., San Martín De Viale, L.C., Heme metabolism after discontinued hexachlorobenzene administration in rats: Possible irreversible changes and biomarker for hexachlorobenzene persistence (2000) Comp Biochem Physiol Part C, 127, pp. 165-175 | ||
| 504 | |a Böger, A., Koss, G., Koransky, W., Naummann, R., Frenzel, H., Rat liver alteration after chronic treatment with hexachlorobenzene (1979) Virchows Arch, 382, pp. 127-137 | ||
| 504 | |a Burr, I.M., Balant, L., Stauffacher, W., Renold, A.E., Grodsky, G., Dynamic aspects of proinsulin release from perifused rat pancreas (1969) Lancet, 2, pp. 882-883 | ||
| 504 | |a Cheng, K.C., Cahill, D.S., Kasai, H., Nishimura, S., Loeb, L.A., 8-Hydroxy-guanine, an abundant form of oxidative DNA damage caused G → T and A → C substitutions (1992) J Biol Chem, 267, pp. 166-172 | ||
| 504 | |a Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A., Smith, F., Colorimetric method for determination of sugar and related compound (1956) Anal Chem, 28, pp. 350-356 | ||
| 504 | |a Elder, G.H., Porphyria cutanea tarda (1998) Semin Liver Dis, 18, pp. 67-75 | ||
| 504 | |a Ennamany, R., Marzetto, S., Saboreaud, D., Creppy, E.E., Lipid peroxidation induced by bolesatine, a toxin of Boletus Satanas: Implications in m5dC variation in Vero cells related to inhibition of cell growth (1995) Cell. Biol. Toxicol., 11, pp. 347-354 | ||
| 504 | |a Fan, F., Rozman, K.K., Relationship between acute toxicity of 2,3,7,8,-tetrachlorodibenzo-p- dioxin (TCDD) and disturbance of intermediary metabolism in the Long-Evans rat (1994) Arch Toxicol, 69, pp. 73-78 | ||
| 504 | |a Fan, F., Rozman, K.K., Short- and long-term biochemical effects of 2,3,7,8-tetrachlorodibenzo-p- dioxin in female Long-Evans rats (1995) Toxicol Lett, 75, pp. 209-216 | ||
| 504 | |a Ferioli, A., Harvey, C., De Matteis, F., Drug induced accumulation of uroporphyrin in chicken hepatocytes cultures (1984) Biochem J, 224, pp. 769-777 | ||
| 504 | |a Gorski, J.R., Rozman, K., Dose-response and time course of hypothyroxinemia and hypoinsulinemia and characterization of insulin hypersensitivity in 2,3,7,8-tetrachlorobenzo-p- dioxin (TCDD) treated rats (1987) Toxicology, 44, pp. 297-307 | ||
| 504 | |a Granner, D.K., Andreone, T.L., Insulin modulation of gene expression (1985) Diabet Metab Rev, 1, pp. 139-170 | ||
| 504 | |a Guyton, K.Z., Lui, Y., Gorospe, M., Xu, Q., Holbrook, N.J., Activation of mitogen- activated protein kinase by H2O 2. Role in cell survival following oxidant injury (1996) J Biol Chem, 271, pp. 4138-4142 | ||
| 504 | |a Hanson, R.W., Regulation of phosphoenolpyruvate carboxykinase (GTP) gene expression (1997) Annu Rev Biochem, 66, pp. 581-611 | ||
| 504 | |a Herbert, V., Lau, K., Gottlieb, C.W., Coated charcoal immunoassay of insulin (1965) J Clin Endocrinol Metab, 25, pp. 1375-1384 | ||
| 504 | |a Hers, H.G., Hue, L., Gluconeogenesis and related aspects of glycolysis (1983) Annu Rev Biochem, 52, pp. 617-653 | ||
| 504 | |a Horváth, M.E., Faux, S.P., Smith, A.G., Blazovics, A., Van Looij, D., Fehér, J., Cheeseman, K.H., Vitamin E protects against iron-hexachlorobenzene induced porphyria and formation of 8-hydroxydeoxyguanosine in liver of C57BL/10 Sc5n mice (2001) Toxicol Lett, 122, pp. 97-102 | ||
| 504 | |a Imamura, K., Tanaka, T., Pyruvate kinase isozymes from rat (1982) Methods in Enzymology, Vol 90: Carbohydrate Metabolism, 90 (PART E), pp. 150-165. , Wood WA (ed). Academic Press, New York | ||
| 504 | |a Ivanov, D.E., Chernev, K., Adjarov, D., Krustev, L., Georgiev, G., The effect of different diets on porphyrin metabolism and microsomal liver enzyme induction in experimental hexachlorobenzene porphyria (1976) Porphyrins in Human Diseases. 1st Int. Porphyrin Meet., pp. 438-444. , Freiburg/Br 1975. Karger, Basle | ||
| 504 | |a Kappas, A., Sassa, S., Galbraith, R.A., Nordmann, Y., The porphyrias (1995) Metabolic and Molecular Basis of Inherited Disease, pp. 2103-2159. , Scriver CR, Beaudet AL, Sly WS, Valle D, Stanbury JB, Wibgaarden JB, Fredickson DS (eds). McGraw-Hill, New York | ||
| 504 | |a Koss, G., Koransky, W., Studies on the toxicology of hexachlorobenzene. I. Pharmacokinetics (1975) Arch Toxicol, 34, pp. 203-212 | ||
| 504 | |a Koss, G., Seubert, S., Seubert, A., Koransky, W., Ippen, H., Studies on the toxicology of hexachlorobenzene. III. Observation in a long term experiment (1978) Arch Toxicol, 40, pp. 285-294 | ||
| 504 | |a Kószó, F., Horvath, L., Simon, N., Siklosi, C., Kiss, M., The role of possible membrane damage in porphyria cutanea tarda: A spin label study of rat liver cell membranes (1982) Biochem Pharmacol, 31, pp. 11-17 | ||
| 504 | |a Kuiper-Goodman, T., Grant, D.L., Moodie, C.A., Korsrud, G.O., Munro, I.C., Subacute toxicity of hexachlorobenzene in the rat (1977) Toxicol Appl Pharmacol, 40, pp. 529-549 | ||
| 504 | |a Lacy, P.E., Kostianovsky, M., Method for the isolation of intact islets of Langerhans from the rat pancreas (1967) Diabetes, 16, pp. 35-39 | ||
| 504 | |a Lim, C.K., Rideout, J.M., Wright, D.J., Separation of porphyrins isomers by high-performance liquid chromatography (1983) Biochem J, 211, pp. 435-438 | ||
| 504 | |a Lowry, O.H., Rosebrough, N.J., Randall, R.J., Protein measurement with the folin phenol reagement (1951) J Biol Chem, 193, pp. 265-275 | ||
| 504 | |a Mollenhauer, H.H., Jhonson, J.H., Younger, R.L., Clark, D.E., Ultraestructural changes in the liver fed rat hexachlorobenzene (1975) Am J Vet Res, 37, pp. 1777-1781 | ||
| 504 | |a Moran, G.R., Fitzpatrick, P.F., A continuous fluorescence assay for trypthophan hydrolase (1999) Anal Biochem, 266, pp. 148-152 | ||
| 504 | |a Morel, Y., Barouki, R., Down-regulation of cytochrome P450 1A1 gene promoter by oxidative stress. Critical contribution of nuclear factor 1 (1998) J Biol Chem, 273, pp. 26969-26976 | ||
| 504 | |a Petrescu, I., Bojan, O., Saied, M., Barzu, O., Schmidt, F., Kühnle, H.F., Determination of phosphoenolpyruvate carboxykinase activity with deoxyguanosine 5′-diphosphate as nucleotide substrate (1979) Anal Biochem, 96, pp. 279-281 | ||
| 504 | |a Pilkis, S.J., Fox, E., Wolfe, L., Rothbarth, L., Colosia, A., Stewart, H.B., El-Maghrabi, M.R., Hormonal modulation of key hepatic regulatory enzymes in the gluconeogenic/glycolytic pathway (1986) Ann NY Acad Sci, 478, pp. 1-19 | ||
| 504 | |a Ríos De Molina, M.C., Iglesias, S., Lauría, L., San Martín De Viale, L.C., Precancerous pathology evoked by hexachlorobenzene treatment (1996) Acta Physiol Pharmacol Latinoam, 46, pp. 71-87 | ||
| 504 | |a Ríos De Molina, M.C., Suarez Lissi, M.A., Armesto, A., Lafourcade, C., Lissi, E., Rat urinary chemoluminiscence (1998) Biolumin Chemilumin, 13, pp. 63-68 | ||
| 504 | |a Rose, J.A., Hellman, E.S., Tschudy, D.P., Effect on diet on induction of experimental porphyria (1961) Metabolism, 10, pp. 514-521 | ||
| 504 | |a Rozman, K., Mueller, W., Coulson, F., Korte, F., Long-term feeding study of hexachlorobenzene in Rhesus monkeys (1977) Chemosphere, 3, pp. 81-84 | ||
| 504 | |a San Martín De Viale, L.C., Tomio, J.M., Ferramola, A.M., Sancovich, A.H., Tigier, H.A., Experimental porphyria induced in rats by hexachlorobenzene. Studies on enzyme associated with heme pathway. Effect of 17-β-estradiol (1975) First Int Porphyrin Meet: Porphyrins in Human Diseases, pp. 453-458. , Doss M (ed). Karger, Basle | ||
| 504 | |a San Martín De Viale, L.C., Ríos De Molina, M.C., Wainstok De Calmanovici, R., Tomio, J.M., Porphyrins and porphyrinogen carboxylyase in hexachlorobenzene induced porphyria (1977) Biochem J, 168, pp. 393-400 | ||
| 504 | |a Smith, A.G., De Matteis, F., Drugs and hepatic porphyrias (1980) Clin Haematol, 9, pp. 399-425 | ||
| 504 | |a Smith, S.A., Pogson, C.I., Tryptophan and the control of plasma glucose concentrations in the rat (1977) Biochem J, 168, pp. 495-506 | ||
| 504 | |a Stahl, B.H., Beer, D.G., Weber, L.W.D., Rozman, K., Reduction of hepatic phosphoenolpyruvate carboxykinase (PEPCK) activity by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is due to decreased mRNA levels (1993) Toxicology, 79, pp. 81-95 | ||
| 504 | |a Tschudy, D.P., Welland, F., Collins, A., Hunter, G., The effect of carbohydrate feeding of δ-aminolevulinic acid synthetase (1964) Metabolism, 13, pp. 396-406 | ||
| 504 | |a Vaena De Avalos, S., Martini, C.N., San Martin De Viale, L.C., Effect of hexachlorobenzene on steroids synthesis in adrenal cortex of rats (1998) Proceedings of the XXXIV Annual Meeting of the Argentinian Society of Biochemistry and Molecular Biology (Mendoza) M, 175, p. 34 | ||
| 504 | |a Wainstok De Calmanovici, R., Rios De Molina, M.C., Tomio, J.M., San Martín De Viale, L.C., Mechanism of hexachlorobenzene induced porphyria in rats. Effect of phenobarbitone pretreatment (1984) Biochem J, 218, pp. 753-763 | ||
| 504 | |a Wainstok De Calmanovici, R., Cochon, A., Aldonatti, C., Cabral, J.R., San Martín De Viale, L.C., Sex comparasion of heme pathway in rays bearing hepatic tumors (1991) Tumori, 77, pp. 379-384 | ||
| 504 | |a Weber, L.W.D., Lebofsky, M., Greim, H., Rozman, K., Key enzymes of gluconeogenesis are dose-dependently reduced in 2,3,7,8-tetrachlodibenzo-p-dioxin (TCDD)-treated rats (1991) Arch Toxicol, 65, pp. 119-123 | ||
| 504 | |a Weber, L.W.D., Stahl, B.U., Rozman, K., Are serotonergic mechanisms involved in the acute toxicity of chlorinated dibenzo-p-dioxins (CDDs) (1992) Chemosphere, 25, pp. 161-164 | ||
| 504 | |a Wilson, J., Kueveruwa, S.S., (1994) Toxicological Profile for Hexachlorobenzene, , Update. US Department of Health and Human Services. Public Health Service Agency for Toxic Substances Disease Registry | ||
| 504 | |a Wolf, W.A., Kuhn, D.M., Simultaneous determination of 5-H TRP, its amino acid precursors and acid metabolite in discrete brain regions by HPLC with fluorescence detection (1983) J Chromatog, 275, pp. 1-9 | ||
| 504 | |a Wolff, S.P., Dean, R.T., Fragmentation of proteins by free radicals and its effect on their susceptibility to enzyme hydrolysis (1986) Biochem J, 234, pp. 299-403 | ||
| 520 | 3 | |a Hexachlobenzene (HCB), one of the most persistent environmental pollutants, induces porphyria cutanea tarda (PCT). The aim of this work was to analyze the effect of HCB on some aspects of glucose metabolism, particularly those related to its neosynthesis in vivo. For this purpose, a time-course study on gluconeogenic enzymes, pyruvate carboxylase (PC), phosphoenolpyruvate carboxykinase (PEPCK), glucose-6-phosphatase (G-6-Pase) and on pyruvate kinase (PK), a glycolytic enzyme, was carried out. Plasma glucose and insulin levels, hepatic glycogen, tryptophan contents, and the pancreatic insulin secretion pattern stimulated by glucose were investigated. Oxidative stress and heme pathway parameters were also evaluated. HCB treatment decreased PC, PEPCK, and G-6-Pase activities. The effect was observed at an early time point and grew as the treatment progressed. Loss of 60, 56, and 37%, respectively, was noted at the end of the treatment when a considerable amount of porphyrins had accumulated in the liver as a result of drastic blockage of uroporphyrinogen decarboxylase (URO-D) (95% inhibition). The plasma glucose level was reduced (one-third loss), while storage of hepatic glucose was stimulated in a time-dependent way by HCB treatment. A decay in the normal plasma insulin level was observed as fungicide intoxication progressed (twice to four times lower). However, normal insulin secretion of perifused pancreatic Langerhans islets stimulated by glucose during the 3rd and 6th weeks of treatment did not prove to be significantly affected. HCB promoted a time-dependent increase in urinary chemiluminiscence (fourfold) and hepatic malondialdehide (MDA) content (fivefold), while the liver tryptophan level was only raised at the longest intoxication times. These results would suggest that HCB treatment does not cause a primary alteration in the mechanism of pancreatic insulin secretion and that the changes induced by the fungicide on insulin levels would be an adaptative response of the organism to stimulate gluconeogenesis. They showed for the first time that HCB causes impairment of the gluconeogenic pathway. Therefore, the reduced levels of glucose would thus be the consequence of decreased gluconeogenesis, enhanced glucose storage, and unaffected glycolysis. The impairment of gluconeogenesis (especially for PEPCK) and the related variation in glucose levels caused by HCB treatment could be a consequence of the oxidative stress produced by the fungicide. Tryptophan adds its effect to this decrease in the higher phases of HCB intoxication, where its levels overcome the control values possibly owing to the drastic decline of URO-D. This derangement of carbohydrates leads porphyric hepatocytes to have lower levels of free glucose. These results contribute to our understanding of the protective and modulatory effect that diets rich in carbohydrates have in hepatic porphyria disease. |l eng | |
| 536 | |a Detalles de la financiación: Universidad de Buenos Aires | ||
| 536 | |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas | ||
| 536 | |a Detalles de la financiación: Acknowledgements This work was supported by grants from the University of Buenos Aires and CONICET. L.C. San Martín de Viale and J.C. Basabe are Scientific Research Career members of the CONICET (Consejo Nacional de Investigaciones Científicas y Técnicas). Procedures involving animals (care and use) were conducted according to international guidelines (Guide for Care and Use of Laboratory Animals, National Research Council, USA, 1996, and the Council of the European Communities Directive, 86/ 609/ECC). | ||
| 593 | |a Depto. de Quim. Biológica, Fac. de Ciencias Exactas y Naturales, Ciudad Universitaria, C1428BGA, Ciudad Autónoma Buenos Aires, Argentina | ||
| 593 | |a Ctro. de Invest. Endocrinologicas, Hospital de Niños, Dr. Ricardo Gutierrez, C1425EDF, Ciudad Autónoma Buenos Aires, Argentina | ||
| 593 | |a O'Higgins 4332, CP 1429, Buenos Aires, Argentina | ||
| 650 | 1 | 7 | |2 spines |a GLUCONEOGENESIS |
| 650 | 1 | 7 | |2 spines |a GLUCONEOGENESIS |
| 690 | 1 | 0 | |a GLUCOSE |
| 690 | 1 | 0 | |a HEXACHLOROBENZENE |
| 690 | 1 | 0 | |a INSULIN |
| 690 | 1 | 0 | |a PORPHYRIA CUTANEA TARDA |
| 690 | 1 | 0 | |a GLUCOSE |
| 690 | 1 | 0 | |a GLUCOSE 6 PHOSPHATASE |
| 690 | 1 | 0 | |a GLYCOGEN |
| 690 | 1 | 0 | |a HEXACHLOROBENZENE |
| 690 | 1 | 0 | |a INSULIN |
| 690 | 1 | 0 | |a PHOSPHOENOLPYRUVATE CARBOXYKINASE (GTP) |
| 690 | 1 | 0 | |a PYRUVATE CARBOXYLASE |
| 690 | 1 | 0 | |a PYRUVATE KINASE |
| 690 | 1 | 0 | |a ANIMAL CELL |
| 690 | 1 | 0 | |a ANIMAL EXPERIMENT |
| 690 | 1 | 0 | |a ANIMAL MODEL |
| 690 | 1 | 0 | |a ANIMAL TISSUE |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a CONTROLLED STUDY |
| 690 | 1 | 0 | |a EVALUATION |
| 690 | 1 | 0 | |a FEMALE |
| 690 | 1 | 0 | |a GLUCOSE BLOOD LEVEL |
| 690 | 1 | 0 | |a GLUCOSE METABOLISM |
| 690 | 1 | 0 | |a GLYCOGEN LIVER LEVEL |
| 690 | 1 | 0 | |a INSULIN BLOOD LEVEL |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a OXIDATIVE STRESS |
| 690 | 1 | 0 | |a PORPHYRIA CUTANEA TARDA |
| 690 | 1 | 0 | |a PRIORITY JOURNAL |
| 690 | 1 | 0 | |a RAT |
| 690 | 1 | 0 | |a ANIMALS |
| 690 | 1 | 0 | |a DISEASE MODELS, ANIMAL |
| 690 | 1 | 0 | |a ENZYME INHIBITORS |
| 690 | 1 | 0 | |a ENZYMES |
| 690 | 1 | 0 | |a FEMALE |
| 690 | 1 | 0 | |a FUNGICIDES, INDUSTRIAL |
| 690 | 1 | 0 | |a GLUCOSE |
| 690 | 1 | 0 | |a GLUCOSE-6-PHOSPHATASE |
| 690 | 1 | 0 | |a HEXACHLOROBENZENE |
| 690 | 1 | 0 | |a LIVER |
| 690 | 1 | 0 | |a PHOSPHOENOLPYRUVATE CARBOXYLASE |
| 690 | 1 | 0 | |a PORPHYRIA CUTANEA TARDA |
| 690 | 1 | 0 | |a PYRUVATE CARBOXYLASE |
| 690 | 1 | 0 | |a PYRUVATE KINASE |
| 690 | 1 | 0 | |a RATS |
| 690 | 1 | 0 | |a RATS, WISTAR |
| 690 | 1 | 0 | |a ANIMALIA |
| 690 | 1 | 0 | |a VERTEBRATA |
| 700 | 1 | |a Taira, M.C. | |
| 700 | 1 | |a Lelli, S.M. | |
| 700 | 1 | |a Dascal, E. | |
| 700 | 1 | |a Basabe, J.C. | |
| 700 | 1 | |a De Viale, L.C.S.M. | |
| 773 | 0 | |d 2004 |g v. 78 |h pp. 25-33 |k n. 1 |p Arch. Toxicol. |x 03405761 |w (AR-BaUEN)CENRE-3820 |t Archives of Toxicology | |
| 856 | 4 | 1 | |u https://www.scopus.com/inward/record.uri?eid=2-s2.0-1442352149&doi=10.1007%2fs00204-003-0470-y&partnerID=40&md5=d8dcee45deeedc8b13716dcea0e83b37 |y Registro en Scopus |
| 856 | 4 | 0 | |u https://doi.org/10.1007/s00204-003-0470-y |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_03405761_v78_n1_p25_Mazzetti |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03405761_v78_n1_p25_Mazzetti |y Registro en la Biblioteca Digital |
| 961 | |a paper_03405761_v78_n1_p25_Mazzetti |b paper |c PE | ||
| 962 | |a info:eu-repo/semantics/article |a info:ar-repo/semantics/artículo |b info:eu-repo/semantics/publishedVersion | ||
| 999 | |c 65655 | ||