Na+/K+-ATPase activity and gill ultrastructure in the hyper-hypo-regulating crab Chasmagnathus granulatus acclimated to dilute, normal, and concentrated seawater
We studied Na+/K+-ATPase activity and ultrastructure in gills of the hyper-hypo-regulating crab Chasmagnathus granulatus Dana, 1851 acclimated to different salinities: 10, 30 and 45‰, known to be hypo-, iso-, and hyper-osmotic to the hemolymph, respectively. After centrifugation of homogenates at 11...
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2004
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| LEADER | 13888caa a22009377a 4500 | ||
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| 001 | PAPER-4697 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203416.0 | ||
| 008 | 190411s2004 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-1642505557 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a MBIOA | ||
| 100 | 1 | |a Genovese, G. | |
| 245 | 1 | 0 | |a Na+/K+-ATPase activity and gill ultrastructure in the hyper-hypo-regulating crab Chasmagnathus granulatus acclimated to dilute, normal, and concentrated seawater |
| 260 | |c 2004 | ||
| 270 | 1 | 0 | |m Genovese, G.; Depto. de Biodiversidad y Biol. Exp., Fac. de Ciencias Exactas y Naturales, Ciudad Universitaria (C1428EHA), Buenos Aires, Argentina; email: genovese@bg.fcen.uba.ar |
| 506 | |2 openaire |e Política editorial | ||
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| 520 | 3 | |a We studied Na+/K+-ATPase activity and ultrastructure in gills of the hyper-hypo-regulating crab Chasmagnathus granulatus Dana, 1851 acclimated to different salinities: 10, 30 and 45‰, known to be hypo-, iso-, and hyper-osmotic to the hemolymph, respectively. After centrifugation of homogenates at 11,000 g, Na+/ K+-ATPase activity was almost entirely found in the pellets from the posterior (6-8) and anterior (3-5) gills, whereas very little was detected in the supernatant liquid. Specific activity of gill 6 was 41.3, 30.2, and 28.2 μmol Pi h-1 mg prot-1 for crabs acclimated to 10, 30, and 45‰, respectively, the result for 10‰ being significantly higher than those at 30 and 45‰. Although the concentration of sodium at which the reaction rate is half-maximal (KM) was similar in the three acclimation salinities, only the enzyme from crabs acclimated to 10‰ was inhibited by high sodium concentration. Specific activity of gill 5 increased with the increment in external salinity (10.1, 15, and 18.1 μmol Pi h-1 mg prot -1 for 10, 30, and 45‰, respectively), the only significant difference being that between the extreme salinities. The epithelium thickness of the dorsal portion of gill 6 showed a variation among salinities: 21.7, 15.8 and 17.2 μm for 10, 30 and 45‰, respectively. There were significant differences in epithelium thickness between the 10‰ and the other salinities. In all three salinities, the ultrastructure of gill 6 epithelium showed a high density of mitochondria, estimated by their volume fraction (Vvm = 0.307-0.355). These mitochondria were packed between extensive basolateral membrane interdigitations in ionocytes and pillar cells. Gill 5 showed three cell types: pillars which possess mitochondria packed between membrane folds only in their interdigitations with neighbouring cells; type-I cells 8.0 μm thick with low density of mitochondria (Vvm = 0.088), and type-II cells, 9.9 μm thick and rich in mitochondria (Vv m = 0.423), but lacking basolateral interdigitations. Vvm of type-I cells of gill 5 was significantly lower than those of type-II cells of the same gill and the ionocytes of gill 6. No significant difference in Vvm was detected between the latter cell types. |l eng | |
| 536 | |a Detalles de la financiación: Universidad de Buenos Aires | ||
| 536 | |a Detalles de la financiación: Instituto Antártico Argentino | ||
| 536 | |a Detalles de la financiación: Acknowledgements This work was supported by a grant, Ubacyt X222, from the Universidad de Buenos Aires and material supplied by the Instituto Antártico Argentino. We wish to acknowledge Dr. Martin Ansaldo and Mrs. Isabel Farías for their kind help, Dr. Horst Onken for critical suggestions and Dr. Alejandra Nudelman for revising the translation from the original Spanish. The authors declare that the experiments comply with the current laws of the country in which the experiments were performed. | ||
| 593 | |a Depto. de Biodiversidad y Biol. Exp., Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina | ||
| 593 | |a Rivadavia 1917 (C1033AAJ), CONICET, Buenos Aires, Argentina | ||
| 593 | |a Depto. de Biodiversidad y Biol. Exp., Fac. de Ciencias Exactas y Naturales, Ciudad Universitaria (C1428EHA), Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a CRAB |
| 690 | 1 | 0 | |a ULTRASTRUCTURE |
| 690 | 1 | 0 | |a CHASMAGNATHUS GRANULATA |
| 690 | 1 | 0 | |a DECAPODA (CRUSTACEA) |
| 700 | 1 | |a Luchetti, C.G. | |
| 700 | 1 | |a Luquet, C.M. | |
| 773 | 0 | |d 2004 |g v. 144 |h pp. 111-118 |k n. 1 |p Mar. Biol. |x 00253162 |w (AR-BaUEN)CENRE-194 |t Marine Biology | |
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