Ca2+ calmodulin-dependent protein kinase activity in the ascomycetes Neurospora crassa
DEAE-cellulose column chromatography of Neurospora crassa soluble mycelial extracts leads to the resolution of three major protein kinase activity peaks designated PKI, PKII, and PKIII. PKII activity is stimulated by Ca2+ and Neurospora or brain calmodulin. Maximal stimulation was observed at 2 μM-f...
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| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
Kluwer Academic Publishers
1991
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| LEADER | 08423caa a22011177a 4500 | ||
|---|---|---|---|
| 001 | PAPER-466 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518202949.0 | ||
| 008 | 190411s1991 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0025921715 | |
| 024 | 7 | |2 cas |a calcium, 7440-70-2; cyclic AMP, 60-92-4; egtazic acid, 67-42-5; protein kinase (calcium,calmodulin), 97350-82-8; protein kinase, 9026-43-1; Ca(2+)-Calmodulin Dependent Protein Kinase, EC 2.7.1.123; Calcium, 7440-70-2; Calmodulin; Cyclic AMP, 60-92-4; Egtazic Acid, 67-42-5; Fungal Proteins; Protein Kinases, EC 2.7.1.37 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a MCBIB | ||
| 100 | 1 | |a Ulloa, R.M. | |
| 245 | 1 | 0 | |a Ca2+ calmodulin-dependent protein kinase activity in the ascomycetes Neurospora crassa |
| 260 | |b Kluwer Academic Publishers |c 1991 | ||
| 270 | 1 | 0 | |m Ulloa, R.M.; Instituto, de Investigaciones en Ingeniería Genética y Biología Molecular (INGEBI-CONICET)Argentina |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Judewicz, N.D., Glikin, G.C., Torres, H.N., Protein kinase activities in Neurospora crassa (1981) Arch Biochem Biophys, 206, pp. 87-92 | ||
| 504 | |a Powers, P.A., Pall, M., Cyclic AMP-dependent protein kinase in Neurospora crassa (1980) Biochem Biophys Res Commun, 95, pp. 701-706 | ||
| 504 | |a Van Tuinen, D., Ortega Pérez, R., Marmé, D., Turian, G., Calcium, calmodulin-dependent protein phosphorylation in Neurospora crassa (1984) FEBS LETT, 176, pp. 317-320 | ||
| 504 | |a Ulloa, R.M., Glikin, G.C., Téllez-Iñón, M.T., Torres, H.N., Judewicz, N.D., A novel stimulator of protein phosphorylation in Neurospora crassa (1987) Mol Cell Biochem, 77, pp. 11-17 | ||
| 504 | |a Favre, B., Turian, G., Identification of a Calcium and Phospholipid-dependent protein kinase (Protein Kinase C) in Neurospora crassa (1987) Plant Science, 49, pp. 15-21 | ||
| 504 | |a Ortega-Pérez, R., Van Tuinen, D., Marmé, D., Turian, G., Calmodulin-stimulated cyclic nucleotide phosphodiesterase from Neurospora crassa (1983) Biochem Biophys Acta, 758, pp. 84-87 | ||
| 504 | |a Téllez-Iñón, M.T., Ulloa, R.M., Glikin, G.C., Torres, HN., Characterization of Neurospora crassa cyclic AMP phosphodiesterase activated by calmodulin (1985) Biochem J, 232, pp. 425-430 | ||
| 504 | |a Reig, J.A., Téllez-Iñón, M.T., Flawiá, M.M., Torres, HN., Activation of Neurospora crassa soluble adenylate cyclase by calmodulin (1984) Biochem J, 221, pp. 541-543 | ||
| 504 | |a Roskoski, R., Jr., Assays of protein kinases (1983) Meth Enzymol, 99, pp. 3-6 | ||
| 504 | |a Lowry, O.H., Rosenborough, N.J., Farr, A.L., Randall, RJ., Protein measurement with the Folin phenol reagent (1951) J Biol Chem, 193, pp. 265-275 | ||
| 504 | |a Kornblihtt, A.R., Flawiá, M.M., Torres, HN., Mn2+-dependent activity of rat testis adenylate cyclase. Purification and properties (1981) Biochemistry, 20, pp. 1262-1267 | ||
| 504 | |a Bartfai, T., Preparation of metal chelate complexes and the design of steady-state kinetics experiments involving metal nucleotide complexes (1979) Adv Cyclic Nucl Res, 10, pp. 219-242 | ||
| 504 | |a Johnson, R.A., Walseth, TF., The enzymatic preparation of [32p]-ATP, [32p]-GTP, [32p]cAMP and [32p]cGMP and their use in assays of adenylate and guanylate cyclases and cyclic nucleotide phosphodiesterase (1979) Adv Cyclic Nucl Res, 10, pp. 135-167 | ||
| 504 | |a Gupa, R.C., Reddy, M.V., Randerath, K., [32P] postlabeling analysis of non-radioactive aromatic carcinogen DNA adducts (1982) Carcinogenesis, 9, pp. 1081-1092 | ||
| 504 | |a Klee, C.B., Krinks, MH., Purification of cyclic 3′, 5′-Nucleotide phosphodiesterase inhibitory protein by affinity chromatography on activated protein coupled to sepharose (1978) Biochemistry, 17, pp. 120-126 | ||
| 504 | |a Wang, J.H., Teo, T.S., Ho, H.C., Stevens, FC., Bovine heart protein activation of cyclic nucleotide phosphodiesterase (1975) Adv Cyclic Nucl Res, 5, pp. 179-194 | ||
| 504 | |a Miller, S.G., Kennedy, MB., Regulation of brain type II Ca2+/calmodulin-dependent protein kinase by autophosphorylation: A Ca2+-triggered molecular switch (1986) Cell, 44, pp. 861-870 | ||
| 504 | |a Bartelt, D.C., Fidel, S., Farber, L.H., Wolff, D.J., Hammell, RL., Calmodulin-dependent multifunctional protein kinase in Aspergillus nidulans (1988) Proc Natl Acad Sci USA, 85, pp. 3279-3283 | ||
| 504 | |a Edelman, A.M., Blumenthal, D.K., Krebs, EG., Protein serine/threonine kinases (1987) Ann Rev Biochem, 56, pp. 567-613 | ||
| 520 | 3 | |a DEAE-cellulose column chromatography of Neurospora crassa soluble mycelial extracts leads to the resolution of three major protein kinase activity peaks designated PKI, PKII, and PKIII. PKII activity is stimulated by Ca2+ and Neurospora or brain calmodulin. Maximal stimulation was observed at 2 μM-free Ca2+ and 1 μg/ml of the modulator. The stimulatory effect of the Ca2+-calmodulin complex was blocked by EGTA and by some calmodulin antagonists such as phenothiazine drugs or compound 48/80. PKII phosphorylates different proteins, among which histone II-A at a low concentration and CDPKS, the synthetic peptide specific for Ca2+-calmodulin dependent protein kinases, are the best substrates. Some phosphorylation can be detected in the absence of any exogenous acceptor. PKII activity assayed in the presence of histone II-A or in the absence of exogenous phosphate acceptor (autophosphorylation) co-elute in a DEAE-cellulose column at 0.28 M NaCl. As result of the autophosphorylation reaction of the purified enzyme a main phosphorylated component of 70 kDa was resolved by SDS-polyacrylamide gel electrophoresis. It is possible that this component is an active part of this enzyme. © 1991 Kluwer Academic Publishers. |l eng | |
| 593 | |a Instituto, de Investigaciones en Ingeniería Genética y Biología Molecular (INGEBI-CONICET), Argentina | ||
| 593 | |a Facultad de Ciencias Exactas y Naturales Obligado 2490, Buenos Aires, 1428, Argentina | ||
| 690 | 1 | 0 | |a AUTOPHOSPHORYLATION |
| 690 | 1 | 0 | |a CA2+ |
| 690 | 1 | 0 | |a CALMODULIN |
| 690 | 1 | 0 | |a KINASE |
| 690 | 1 | 0 | |a NEUROSPORA CRASSA |
| 690 | 1 | 0 | |a PROTEIN KINASE (CALCIUM,CALMODULIN) |
| 690 | 1 | 0 | |a CA(2+) CALMODULIN DEPENDENT PROTEIN KINASE |
| 690 | 1 | 0 | |a CALCIUM |
| 690 | 1 | 0 | |a CALMODULIN |
| 690 | 1 | 0 | |a CYCLIC AMP |
| 690 | 1 | 0 | |a EGTAZIC ACID |
| 690 | 1 | 0 | |a FUNGAL PROTEIN |
| 690 | 1 | 0 | |a PROTEIN KINASE |
| 690 | 1 | 0 | |a PROTEIN KINASE (CALCIUM,CALMODULIN) |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a AUTOPHOSPHORYLATION |
| 690 | 1 | 0 | |a NEUROSPORA CRASSA |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a PRIORITY JOURNAL |
| 690 | 1 | 0 | |a ENZYMOLOGY |
| 690 | 1 | 0 | |a GEL CHROMATOGRAPHY |
| 690 | 1 | 0 | |a ION EXCHANGE CHROMATOGRAPHY |
| 690 | 1 | 0 | |a ISOLATION AND PURIFICATION |
| 690 | 1 | 0 | |a NEUROSPORA CRASSA |
| 690 | 1 | 0 | |a PHOSPHORYLATION |
| 690 | 1 | 0 | |a POLYACRYLAMIDE GEL ELECTROPHORESIS |
| 690 | 1 | 0 | |a PROTEIN PROCESSING |
| 690 | 1 | 0 | |a ASCOMYCOTA |
| 690 | 1 | 0 | |a NEUROSPORA |
| 690 | 1 | 0 | |a NEUROSPORA CRASSA |
| 690 | 1 | 0 | |a CA(2+)-CALMODULIN DEPENDENT PROTEIN KINASE |
| 690 | 1 | 0 | |a CALCIUM |
| 690 | 1 | 0 | |a CALMODULIN |
| 690 | 1 | 0 | |a CHROMATOGRAPHY, DEAE-CELLULOSE |
| 690 | 1 | 0 | |a CHROMATOGRAPHY, GEL |
| 690 | 1 | 0 | |a CYCLIC AMP |
| 690 | 1 | 0 | |a EGTAZIC ACID |
| 690 | 1 | 0 | |a ELECTROPHORESIS, POLYACRYLAMIDE GEL |
| 690 | 1 | 0 | |a FUNGAL PROTEINS |
| 690 | 1 | 0 | |a NEUROSPORA CRASSA |
| 690 | 1 | 0 | |a PHOSPHORYLATION |
| 690 | 1 | 0 | |a PROTEIN KINASES |
| 690 | 1 | 0 | |a PROTEIN PROCESSING, POST-TRANSLATIONAL |
| 690 | 1 | 0 | |a SUPPORT, NON-U.S. GOV'T |
| 700 | 1 | |a Torres, H.N. | |
| 700 | 1 | |a Ochatt, C.M. | |
| 700 | 1 | |a Téllez-Iñón, M.T. | |
| 773 | 0 | |d Kluwer Academic Publishers, 1991 |g v. 102 |h pp. 155-163 |k n. 2 |p Mol Cell Biochem |x 03008177 |w (AR-BaUEN)CENRE-3498 |t Molecular and Cellular Biochemistry | |
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| 856 | 4 | 0 | |u https://doi.org/10.1007/BF00234573 |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_03008177_v102_n2_p155_Ulloa |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03008177_v102_n2_p155_Ulloa |y Registro en la Biblioteca Digital |
| 961 | |a paper_03008177_v102_n2_p155_Ulloa |b paper |c PE | ||
| 962 | |a info:eu-repo/semantics/article |a info:ar-repo/semantics/artículo |b info:eu-repo/semantics/publishedVersion | ||
| 963 | |a VARI | ||
| 999 | |c 61419 | ||