Isolation, culture and characterization of a new strain of Euglena gracilis

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A new strain of Euglena gracilis Klebs has been isolated from a highly polluted river; it was named MAT. Strain growth in different culture media was evaluated under heterotrophic and autotrophic conditions. Total lipid, sugar, protein and chlorophyll a production were studied. Results obtained for...

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Detalles Bibliográficos
Autor principal: Ruiz, L.B
Otros Autores: Rocchetta, I., Dos Santos Ferreira, V., Conforti, V.
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: Blackwell Publishing 2004
Acceso en línea:Registro en Scopus
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Biblioteca Central Dr. Luis F. Leloir (FCEN) de Universidad de Buenos Aires
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100 1 |a Ruiz, L.B. 
245 1 0 |a Isolation, culture and characterization of a new strain of Euglena gracilis 
260 |b Blackwell Publishing  |c 2004 
270 1 0 |m Ruiz, L.B.; Depto. de Biodiversidad Y Biol. Exp., Fac. de Ciencias Exactas Y Naturales, Ciudad Universitaria, Pab. II, 1428, Buenos Aires, Argentina; email: lruiz@bg.fcen.uba.ar 
506 |2 openaire  |e Política editorial 
504 |a (1975) Standard Methods for the Examination of Water and Wastewaters, 14th Edn., , APHA, AWWA, WPCF, Washington 
504 |a Barsanti, L., Vismara, R., Passarelli, V., Gualtieri, P., Paramylon (β-1,3-glucan) content in wild type and WZSL mutant of Euglena gracilis: Effects of growth conditions (2000) J. Appl. Phycol., 10, pp. 1-6 
504 |a Bradford, M., A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye-binding (1976) Anal. Biochem., 72, pp. 248-254 
504 |a Buetow, D., (1982) The Biology of Euglena, 3, pp. 200-201. , Academic Press, New York 
504 |a (1988) Catalogue of Strains, 19p. , Natural Environment Research Council, Thompson, A. S., Rhodes, J. C. and Pettman, I (Eds). Culture Collection of Algae and Protozoa, Cumbria 
504 |a Conforti, V., Taxonomic study of the Euglenophyta of a highly polluted river of Argentina (1991) Nova Hedwigia, 53, pp. 73-98 
504 |a Conforti, V., Alberghina, J., Gonzalez Urda, E., Structural change and dynamics of the phytoplankton along a highly polluted lowland river of Argentina (1995) J. Aquat. Ecosyst. Health, 4, pp. 59-75 
504 |a Devars, S., Torres Marquez, M.E., Gonzalez Halphen, D., Uribe, A., Moreno Sanchez, R., Cyanidine sensitive and cyanide resistant respiration of dark grown Euglena gracilis (1992) Plant Sci., 82, pp. 37-43 
504 |a Dubois, M., Gills, K., Hamilton, J., Rebers, P., Smith, F., Colorimetric method of determination of sugars and related substances (1956) Anal. Chem., 28, pp. 350-356 
504 |a Einecker Lamas, M., Soares, M.J., Oliveira, M.M., Effects of cadmium on Euglena gracilis membrane lipids (1996) Braz. J. Med. Biol. Res., 29, pp. 941-948 
504 |a Fasulo, M.P., Bassi, M., Donini, A., Cytotoxic effects of hexavalent chromium in Euglena gracilis, II. Physiological and ultrastructural studies (1983) Protoplasma, 114, pp. 35-43 
504 |a Gojdics, M., (1953) The Genus Euglena, 268p. , University of Wisconsin Press, Madison 
504 |a Hosotani, K., Yokota, A., Nakano, Y., Kitaoka, S., The metabolic pathway of propionate in Euglena gracilis Z grown under illumination (1980) Agric. Biol. Chem., 44, pp. 1097-1103 
504 |a Kempner, E.S., Miller, J.H., The molecular biology of Euglena gracilis. VII. Inorganic requirements for a minimal culture medium (1972) J. Protozool., 19, pp. 343-346 
504 |a Mackerth, F., Hiron, J., Tailing, J., Water analysis: Some revised methods for limnologists (1978) Freshwater Biol. Assoc. Sci. Pub., 36, p. 117 
504 |a Mayer, S., Beale, S., Light regulation of alpha-aminolevulinic acid biosynthetic enzymes and tRNA in Euglena gracilis (1990) Plant Physiol., 94, pp. 1365-1375 
504 |a Nudelman, A., Rossi, M.S., Conforti, V., Triemer, R.E., Phylogeny of Euglenophyceae based on small subunit rDNA sequences: Taxonomic implications (2003) J. Phycol., 39, pp. 226-235 
504 |a Oliveira, M.M., Alves, M.F., Brigante, A.A., Differences in phospholipid composition of autotrophic and heterotrophic Euglena gracilis (1990) Braz. J. Med. Biol. Res., 23, pp. 1237-1241 
504 |a Pringsheim, E.G., The biphasic or soil-water culture method for growing algae and flagellates (1946) J. Ecol., 33, pp. 193-204 
504 |a Rocchetta, I., Ruiz, L.B., Magaz, G., Conforti, V., Effects of hexavalent chromium in two strains of Euglena gracilis (2003) Bull. Environ. Contam. Toxicol., 70, pp. 1045-1051 
504 |a Sokal, R.R., Rohlf, F.J., (1984) Introducción a la Bioestadtstica, 362p. , Serie de Biología Fundamental. Reverté, España 
504 |a Ternetz, C., Beiträge zur Morphologie und Physiologie der Euglena gracilis Klebs (1912) Jahrb. Wiss. Bot., 51, p. 435 
504 |a (1985) Short Term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms, pp. 76-103. , EPA/600/ 4-85/014/. US EPA Office of water, Washington DC 
504 |a Venrick, E.L., How many cells to count? (1978) Monographs on Oceanographic Methodology. Phytoplankton Manual, pp. 167-180. , Sournia, A. (Ed.) UNESCO Press, Paris 
504 |a Zumstein, H., Morphologie und Physiologie der Euglena gracilis Klebs (1900) Jahrb. Wiss. Bot., 39, p. 1495 
520 3 |a A new strain of Euglena gracilis Klebs has been isolated from a highly polluted river; it was named MAT. Strain growth in different culture media was evaluated under heterotrophic and autotrophic conditions. Total lipid, sugar, protein and chlorophyll a production were studied. Results obtained for MAT were compared with data obtained for a UTEX Culture Collection strain. Likewise, cells from both strains were bleached using streptomycin, and grown in the same media used for green samples. Both MAT and UTEX showed clear differences in their biochemical composition and growth rate depending on the media used. They also exhibited different growth patterns. E. gracilis medium proved to be the best culture environment for both strains either in autotrophic or heterotrophic conditions. Results show that basal contents of lipids, sugars, proteins and chlorophyll a vary depending on the strain, and thus values obtained for one strain do not apply to another. Moreover, strain origin may have an influence on the mechanisms of adaptation or defense developed by each sample.  |l eng 
593 |a Depto. de Biodiversidad Y Biol. Exp., Fac. de Ciencias Exactas Y Naturales, Ciudad Universitaria, Pab. II, 1428, Buenos Aires, Argentina 
690 1 0 |a BLEACHED 
690 1 0 |a CULTURE 
690 1 0 |a EUGLENA GRACILIS 
690 1 0 |a LIPID 
690 1 0 |a PROTEIN 
690 1 0 |a STRAIN 
690 1 0 |a SUGAR 
690 1 0 |a FLAGELLATE 
690 1 0 |a RIVER POLLUTION 
690 1 0 |a EUGLENA 
690 1 0 |a EUGLENA GRACILIS 
690 1 0 |a EUGLENA GRACILIS 
690 1 0 |a MASTIGOPHORA (FLAGELLATES) 
700 1 |a Rocchetta, I. 
700 1 |a Dos Santos Ferreira, V. 
700 1 |a Conforti, V. 
773 0 |d Blackwell Publishing, 2004  |g v. 52  |h pp. 168-173  |k n. 2  |p Phycol. Res.  |x 13220829  |w (AR-BaUEN)CENRE-6446  |t Phycological Research 
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856 4 0 |u https://doi.org/10.1111/j.1440-1835.2004.tb00325.x  |y DOI 
856 4 0 |u https://hdl.handle.net/20.500.12110/paper_13220829_v52_n2_p168_Ruiz  |y Handle 
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