Studies on the decrease of liver cytochrome P-450 content by triiodothyronine

In the rat liver, the microsomal content of cytochrome P-450 decreased by 50% after triiodothyronine (T3) administration. The molecular basis for the decreased cytochrome P-450 levels was investigated. The activities of the enzymes involved in heme synthesis or degradation were not altered by thyroi...

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Autor principal: Kleiman de Pisarev, D.L
Otros Autores: Ferramola de Sancovich, A.M, Sancovich, H.A
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 1991
Acceso en línea:Registro en Scopus
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Registro en la Biblioteca Digital
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024 7 |2 scopus  |a 2-s2.0-0025774218 
024 7 |2 cas  |a 5-Aminolevulinate Synthetase, EC 2.3.1.37; Aminolevulinic Acid, 106-60-5; Cytochrome P-450 Enzyme System, 9035-51-2; Heme, 14875-96-8; Hydroxymethylbilane Synthase, EC 4.3.1.8; Porphobilinogen Synthase, EC 4.2.1.24; Proteins; Serum Albumin; Triiodothyronine, 6893-02-3 
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100 1 |a Kleiman de Pisarev, D.L. 
245 1 0 |a Studies on the decrease of liver cytochrome P-450 content by triiodothyronine 
260 |c 1991 
270 1 0 |m Kleiman de Pisarev, D.L. 
506 |2 openaire  |e Política editorial 
504 |a Oppenheimer, (1979) Science, 203, p. 971 
504 |a Sterling, (1979) N. Engl. J. Med., 300, pp. 117-173 
504 |a Skett, Weir, (1983) Biochem. Pharmacol., 32, pp. 1061-1067 
504 |a Kleiman de Pisarev, Ferramola de Sancovich, Sancovich, (1988) Acta Physiol. Pharmacol. Latinoam., 38, pp. 301-308 
504 |a Waxman, Morrissey, Leblane, (1989) Mol. Pharmacol., 35, pp. 519-525 
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520 3 |a In the rat liver, the microsomal content of cytochrome P-450 decreased by 50% after triiodothyronine (T3) administration. The molecular basis for the decreased cytochrome P-450 levels was investigated. The activities of the enzymes involved in heme synthesis or degradation were not altered by thyroid hormone administration. The incorporation of 3H-δ-aminolaevulinate into the liver microsomal heme was markedly reduced in T3-treated rats. The latter appeared not to reflect a lowered binding affinity of the apoprotein moiety of cytochrome P-450 for heme. The sodium dodecyl sulfate gel electrophoresis of the microsomal preparation showed a decrease in apocytochrome P-450. It is suggested that the amount of the apocytochrome may be the primary event affected in the formation of cytochrome P-450, by triiodothyronine treatment of thyroidectommized rats. © 1991.  |l eng 
536 |a Detalles de la financiación: Umweltbundesamt 
536 |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas 
536 |a Detalles de la financiación: We thank Mrs. Lilia Lescano for her technical assistance. This work was supported in part by grants from the CONICET and UBA. The authors are Career Scientific Researchers in CONICET (Consejo Nacional de lnvestigaciones Cientlficas y T~cnicas). 
593 |a Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Núnez, Buenos Aires, Argentina 
690 1 0 |a (RAT LIVER) 
690 1 0 |a CYTOCHROME P-450 
690 1 0 |a TRIIODOTHYRONINE 
690 1 0 |a CYTOCHROME P450 
690 1 0 |a LIOTHYRONINE 
690 1 0 |a ANIMAL EXPERIMENT 
690 1 0 |a ARTICLE 
690 1 0 |a HYPOTHYROIDISM 
690 1 0 |a INTRAPERITONEAL DRUG ADMINISTRATION 
690 1 0 |a LIVER LEVEL 
690 1 0 |a LIVER MICROSOME 
690 1 0 |a NONHUMAN 
690 1 0 |a PRIORITY JOURNAL 
690 1 0 |a RAT 
690 1 0 |a THYROIDECTOMY 
690 1 0 |a 5-AMINOLEVULINATE SYNTHETASE 
690 1 0 |a AMINOLEVULINIC ACID 
690 1 0 |a ANIMAL 
690 1 0 |a CYTOCHROME P-450 ENZYME SYSTEM 
690 1 0 |a ELECTROPHORESIS, POLYACRYLAMIDE GEL 
690 1 0 |a HEME 
690 1 0 |a HUMAN 
690 1 0 |a HYDROXYMETHYLBILANE SYNTHASE 
690 1 0 |a MALE 
690 1 0 |a MICROSOMES, LIVER 
690 1 0 |a PORPHOBILINOGEN SYNTHASE 
690 1 0 |a PROTEINS 
690 1 0 |a RATS 
690 1 0 |a RATS, INBRED STRAINS 
690 1 0 |a SERUM ALBUMIN 
690 1 0 |a SUPPORT, NON-U.S. GOV'T 
690 1 0 |a TRIIODOTHYRONINE 
690 1 0 |a ANIMALIA 
700 1 |a Ferramola de Sancovich, A.M. 
700 1 |a Sancovich, H.A. 
773 0 |d 1991  |g v. 1074  |h pp. 172-177  |k n. 1  |p Biochim. Biophys. Acta Gen. Subj.  |x 03044165  |w (AR-BaUEN)CENRE-2260  |t BBA - General Subjects 
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