Ensemble and single particle photophysical properties (two-photon excitation, anisotropy, FRET, lifetime, spectral conversion) of commercial quantum dots in solution and in live cells

In this work, we characterized streptavidin-conjugated quantum dots (QDs) manufactured by Quantum Dot Corporation. We present data on: (1) two-photon excitation; (2) fluorescence lifetimes; (3) ensemble and single QD emission anisotropy; (4) QDs as donors for Förster resonance energy transfer (FRET)...

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Autor principal: Grecco, H.E
Otros Autores: Lidke, K.A, Heintzmann, R., Lidke, D.S, Spagnuolo, C., Martinez, O.E, Jares-Erijman, E.A, Jovin, T.M
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 2004
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Acceso en línea:Registro en Scopus
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024 7 |2 scopus  |a 2-s2.0-14144255433 
024 7 |2 cas  |a streptavidin, 9013-20-1; transferrin, 82030-93-1; Fluorescent Dyes; Receptors, Growth Factor; Transferrin, 11096-37-0 
040 |a Scopus  |b spa  |c AR-BaUEN  |d AR-BaUEN 
030 |a MRTEE 
100 1 |a Grecco, H.E. 
245 1 0 |a Ensemble and single particle photophysical properties (two-photon excitation, anisotropy, FRET, lifetime, spectral conversion) of commercial quantum dots in solution and in live cells 
260 |c 2004 
270 1 0 |m Grecco, H.E.; Dept. of Physics, FCEN, University of Buenos Aires, C1428EHA Buenos Aires, Argentina; email: hgrecco@df.uba.ar 
506 |2 openaire  |e Política editorial 
504 |a Alivisatos, P., The use of nanocrystals in biological detection (2004) Nat Biotechnol, 22, pp. 47-52 
504 |a Cantele, G., Calculation of the infrared optical transitions in semiconductor ellipsoidal quantum dots (2001) Nano Lett, 1, pp. 121-124 
504 |a Cantele, G., Piacente, G., Ninno, D., Iadonisi, G., Optical anisotropy of ellipsoidal quantum dots (2002) Phys Rev B, 66, p. 113308 
504 |a Chan, W.C., Nie, S., Quantum dot bioconjugates for ultrasensitive nonisotopic detection (1998) Science, 281, pp. 2016-2018 
504 |a Chung, I., Shimizu, K.T., Bawendi, M.G., Room temperature measurements of the 3D orientation of single CdSe quantum dots using polarization microscopy (2003) Proc Natl Acad Sci USA, 100, pp. 405-408 
504 |a Clapp, A.R., Medintz, I.L., Mauro, J.M., Fisher, B.R., Bawendi, M.G., Mattoussi, H., Fluorescence resonance energy transfer between quantum dot donors and dye-labeled protein acceptors (2004) J Am Chem Soc, 126, pp. 301-310 
504 |a Clayton, A.H.A., Hanley, Q.S., Arndt-Jovin, D.J., Subramaniam, V., Jovin, T.M., Dynamic fluorescence anisotropy imaging microscopy in the frequency domain (rFLIM) (2002) Biophys J, 83, pp. 1631-1649 
504 |a Clayton, A.H., Hanley, Q.S., Verveer, P.J., Graphical representation and multicomponent analysis of single-frequency fluorescence lifetime imaging microscopy data (2004) J Microsc, 213, pp. 1-5 
504 |a Efros, A.L., Rosen, M., Kuno, M., Nirmal, M., Norris, D.J., Bawendi, M.G., Band-edge exciton in quantum dots of semiconductors with a degenerate valence band: Dark and bright exciton states (1996) Phys Rev B, 54, pp. 4843-4856 
504 |a Empedocles, S.A., Neuhauser, R., Bawendi, M.G., Three-dimensional orientation measurements of symmetric single chromophores using polarization microscopy (1999) Nature, 399, pp. 126-130 
504 |a Green, M., Semiconductor quantum dots as biological imaging agents (2004) Angew Chem Int Ed, 43, pp. 4129-4131 
504 |a Hanley, Q.S., Subramaniam, V., Arndt-Jovin, D.J., Jovin, T.M., Fluorescence lifetime imaging: Multi-point calibration, minimum resolvable differences, and artifact suppression (2001) Cytometry, 43, pp. 248-260 
504 |a Henini, M., Sanguetti, S., Fortina, S.C., Grilli, E., Guzzi, M., Panzarini, G., Andreani, L.C., Eaves, L., Optical anisotropy in arrow-shaped InAs quantum dots (1998) Phys Rev B, 57, pp. R6815-R6818 
504 |a Jaiswal, J.K., Simon, S.M., Potentials and pitfalls of fluorescent quantum dots for biological imaging (2004) Trends Cell Biol, 14, pp. 497-504 
504 |a Jares-Erijman, E.A., Jovin, T.M., FRET imaging (2003) Nat Biotechnol, 21, pp. 1387-1395 
504 |a Jensen, B., Torabi, A., Refractuve index of hexagonal II-VI compounds CdSe, CdS, and CdSe xS1-x (1986) J Opt Soc Am B, 3, pp. 857-863 
504 |a Jares-Erijman, E.A., Giordano, L., Spagnuolo, C., Lidke, K.A., Jovin, T.M., Imaging quantum dots switched on and off by photochromic Fluorescence Resonance Energy Transfer (pcFRET) (2004) Mol Cryst Liq Cryst, , in press 
504 |a Larson, D.R., Zipfel, W.R., Williams, R.M., Clark, S.W., Bruchez, M.P., Wise, F.W., Webb, W.W., Water-soluble quantum dots for multiphoton fluorescence imaging in vivo (2003) Science, 300, pp. 1434-1436 
504 |a Lidke, D.S., Arndt-Jovin, D.J., Imaging takes a quantum leap (2004) Physiology, 19, pp. 322-325 
504 |a Lidke, D.S., Nagy, P., Heintzmann, R., Arndt-Jovin, D.J., Post, J.N., Grecco, H., Jares-Erijman, E.A., Jovin, T.M., Quantum dot ligands provide new insights into erbB/HER receptor-mediated signal transduction (2004) Nat Biotechnol, 22, pp. 198-203 
504 |a Medintz, I.L., Clapp, A.R., Mattoussi, H., Goldman, E.R., Fisher, B.R., Mauro, J.M., Self-assembled nanoscale biosensors based on quantum dot FRET donors (2003) Nat Mat, 2, pp. 630-638 
504 |a Medintz, I.L., Konnert, J.H., Clapp, A.R., Stanish, I., Twigg, M.E., Mattoussi, H., Mauro, J.M., Deschamps, J.R., A fluorescence resonance energy transfer-derived structure of a quantum dot-protein bioconjugate nanoassembly (2004) Proc Natl Acad Sci USA, 101, pp. 9612-9617 
504 |a Medintz, I.L., Trammell, S.A., Mattoussi, H., Mauro, J.M., Reversible modulation of quantum dot photoluminescence using a protein-bound photochromic fluorescence resonance energy transfer acceptor (2004) J Am Chem Soc, 126, pp. 30-31 
504 |a Michalet, X., Finaud, F., Lacoste, T.D., Dahan, M., Bruchez, M.P., Alivisatos, A.P., Weiss, S., Properties of fluorescent semiconductor nanocrystals and their application to biological labeling (2001) Single Mol, 2, pp. 261-276 
504 |a Norris, D.J., Bawendi, M.G., Structure in the lowest absorption feature of CdSe quantum dots (1995) J Chem Phys, 103, pp. 5260-5268 
504 |a Norris, D.J., Bawendi, M.G., Measurement and assignment of the size-dependent optical spectrum in Cdse quantum dots (1996) Phys Rev B, 53, pp. 16338-16346 
504 |a Parak, W.J., Gerion, D., Pellegrino, T., Zanchet, D., Micheel, C., Williams, S.C., Boudreau, R., Alivisatos, A.P., Biological applications of colloidal nanocrystals (2003) Nanotechnology, 14, pp. R15-R27 
504 |a Riegler, J., Nann, T., Application of luminescent nanocrystals as labels for biological molecules (2004) Anal Bioanal Chem, 379, pp. 913-919 
504 |a Subramaniam, V., Hanley, Q.S., Clayton, A.H.A., Jovin, T.M., Photophysics of green and red fluorescent proteins: Some implications for quantitative microscopy (2003) Methods Enzymol, 360, pp. 178-201 
504 |a Sark, W., Frederix, P., Bol, A.A., Gerritsen, H., Meijerink, A., Blueing, bleaching, and blinking of single CdSe/ZnS quantum dots (2002) ChemPhysChem, 3, pp. 871-879 
504 |a Yarden, Y., Sliwkowski, M., Untangling the ErbB signalling network (2001) Nat Rev Mol Cell Biol, 2, pp. 127-137 
520 3 |a In this work, we characterized streptavidin-conjugated quantum dots (QDs) manufactured by Quantum Dot Corporation. We present data on: (1) two-photon excitation; (2) fluorescence lifetimes; (3) ensemble and single QD emission anisotropy; (4) QDs as donors for Förster resonance energy transfer (FRET); and (5) spectral conversion of QDs exposed to high-intensity illumination. We also demonstrate the utility of QDs for (1) imaging the binding and uptake of biotinylated transferrin on living cells, and (2) resolving by fluorescence lifetime imaging microscopy (FLIM) signals originating from QDs from those of spatially and spectrally overlapping visible fluorescent proteins (VFPs). © 2005 Wiley-Liss, Inc.  |l eng 
593 |a Departamento de Física, Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, C1428EHA Buenos Aires, Argentina 
593 |a Department of Molecular Biology, Max Planck Inst. Biophysical Chem., D-37070 Göttingen, Germany 
593 |a Departamento de Quimica Organica, Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, C1428EHA Buenos Aires, Argentina 
593 |a Dept. of Physics, FCEN, University of Buenos Aires, C1428EHA Buenos Aires, Argentina 
593 |a Dept. of Molecular Biology, Max Planck Inst. Biophysical Chem., D-37070 Göttingen, Germany 
690 1 0 |a FLUORESCENCE MICROSCOPY 
690 1 0 |a GROWTH FACTOR RECEPTORS 
690 1 0 |a NANOPARTICLES 
690 1 0 |a EPIDERMAL GROWTH FACTOR RECEPTOR 
690 1 0 |a GREEN FLUORESCENT PROTEIN 
690 1 0 |a GROWTH FACTOR RECEPTOR 
690 1 0 |a HYBRID PROTEIN 
690 1 0 |a NANOPARTICLE 
690 1 0 |a QUANTUM DOT 
690 1 0 |a STREPTAVIDIN 
690 1 0 |a TRANSFERRIN RECEPTOR 
690 1 0 |a FLUORESCENT DYE 
690 1 0 |a NANOMATERIAL 
690 1 0 |a TRANSFERRIN 
690 1 0 |a ANISOTROPY 
690 1 0 |a ARTICLE 
690 1 0 |a CANCER CELL CULTURE 
690 1 0 |a CONFOCAL LASER MICROSCOPY 
690 1 0 |a FLUORESCENCE 
690 1 0 |a FLUORESCENCE LIFETIME IMAGING MICROSCOPY 
690 1 0 |a FLUORESCENCE MICROSCOPY 
690 1 0 |a FLUORESCENCE RESONANCE ENERGY TRANSFER 
690 1 0 |a HUMAN 
690 1 0 |a HUMAN CELL 
690 1 0 |a IMAGING SYSTEM 
690 1 0 |a IRRADIATION 
690 1 0 |a MICROSCOPY 
690 1 0 |a MULTIPHOTON MICROSCOPY 
690 1 0 |a PHOTON 
690 1 0 |a PRIORITY JOURNAL 
690 1 0 |a SIGNAL TRANSDUCTION 
690 1 0 |a SQUAMOUS CELL CARCINOMA 
690 1 0 |a TWO PHOTON EXCITATION MICROSCOPY 
690 1 0 |a FLUORESCENCE MICROSCOPY 
690 1 0 |a FLUORESCENCE RESONANCE ENERGY TRANSFER 
690 1 0 |a METABOLISM 
690 1 0 |a TUMOR CELL LINE 
690 1 0 |a ANISOTROPY 
690 1 0 |a CELL LINE, TUMOR 
690 1 0 |a FLUORESCENCE RESONANCE ENERGY TRANSFER 
690 1 0 |a FLUORESCENT DYES 
690 1 0 |a HUMANS 
690 1 0 |a MICROSCOPY, FLUORESCENCE 
690 1 0 |a NANOSTRUCTURES 
690 1 0 |a PHOTONS 
690 1 0 |a QUANTUM DOTS 
690 1 0 |a RECEPTORS, GROWTH FACTOR 
690 1 0 |a SIGNAL TRANSDUCTION 
690 1 0 |a TRANSFERRIN 
653 0 0 |a Carl Zeiss LSM310 confocal laser scanning microscope, Zeiss; Nikon E600 epi-fluorescent microscope, Nikon 
700 1 |a Lidke, K.A. 
700 1 |a Heintzmann, R. 
700 1 |a Lidke, D.S. 
700 1 |a Spagnuolo, C. 
700 1 |a Martinez, O.E. 
700 1 |a Jares-Erijman, E.A. 
700 1 |a Jovin, T.M. 
773 0 |d 2004  |g v. 65  |h pp. 169-179  |k n. 4-5  |p Microsc. Res. Tech.  |x 1059910X  |t Microscopy Research and Technique 
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