Protective effect of a natural carrageenan on genital herpes simplex virus infection in mice

In the present study, the protective effect of 1T1, a λ-carrageenan extracted from the red seaweed Gigartina skottsbergii was evaluated in a murine model of herpes simplex virus type 2 (HSV-2) genital infection. Six to eight-week-old female BALB/c mice were intravaginally inoculated with a lethal do...

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Autor principal: Carlucci, M.J
Otros Autores: Scolaro, L.A, Noseda, M.D, Cerezo, A.S, Damonte, E.B
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 2004
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Acceso en línea:Registro en Scopus
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Registro en la Biblioteca Digital
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024 7 |2 cas  |a carrageenan, 9000-07-1, 9049-05-2, 9061-82-9, 9064-57-7; Antiviral Agents; Carrageenan, 9000-07-1 
040 |a Scopus  |b spa  |c AR-BaUEN  |d AR-BaUEN 
030 |a ARSRD 
100 1 |a Carlucci, M.J. 
245 1 0 |a Protective effect of a natural carrageenan on genital herpes simplex virus infection in mice 
260 |c 2004 
270 1 0 |m Laboratorio de Virología, Depto. de Quím. Biol., Fac. Cie. Exact. Y Nat., Univ. B.Argentina; email: edamonte@qb.fcen.uba.ar 
506 |2 openaire  |e Política editorial 
504 |a Arvin, A.M., Prober, C.G., Herpes simplex virus type 2 - A persistent problem (1997) N. Engl. J. Med., 337, pp. 1158-1159 
504 |a Baba, M., Pauwels, R., Balzarini, J., Arnout, J., Desmyter, J., De Clercq, E., Mechanism of inhibitory effect of dextran sulfate and heparin on replication of human immunodeficiency virus in vitro (1988) Proc. Natl. Acad. Sci. U.S.A., 85, pp. 6132-6136 
504 |a Bourne, K.Z., Bourne, N., Reising, S.F., Stanberry, L.R., Plant products as topical microbicide candidates: Assessment of in vitro and in vivo activity against herpes simplex virus type 2 (1999) Antiviral Res., 42, pp. 219-226 
504 |a Bourne, N., Bernstein, D.I., Ireland, J., Sonderfan, A.J., Profy, A.T., Stanberry, L.R., The topical microbicide PRO 2000 protects against genital herpes infection in a mouse model (1999) J. Infect. Dis., 180, pp. 203-205 
504 |a Carlucci, M.J., Pujol, C.A., Ciancia, M., Noseda, M.D., Matulewicz, M.C., Damonte, E.B., Cerezo, A.S., Antiherpetic and anticoagulant properties of carrageenans from the red seaweed Gigartina skottsbergii and their cyclized derivatives: Correlation between structure and biological activity (1997) Int. J. Biol. Macromol., 20, pp. 97-105 
504 |a Carlucci, M.J., Ciancia, M., Matulewicz, M.C., Cerezo, A.S., Damonte, E.B., Antiherpetic activity and mode of action of natural carrageenans of diverse structural types (1999) Antiviral Res., 43, pp. 93-102 
504 |a Carlucci, M.J., Scolaro, L.A., Damonte, E.B., Inhibitory action of natural carrageenans on herpes simplex virus infection of mouse astrocytes (1999) Chemotherapy, 45, pp. 429-436 
504 |a Coggins, C., Blanchard, K., Alvarez, B., Brache, V., Weisberg, E., Kilmarx, P.H., Lacarra, M., Ellertson, C., Preliminary safety and acceptability of a carrageenan gel for possible use as a vaginal microbicide (2000) Sex. Transm. Infect., 76, pp. 480-483 
504 |a Cohen, M.S., Sexually transmitted diseases enhance HIV transmission: No longer a hypothesis (1998) Lancet, 351, pp. 5-7 
504 |a Corey, L., Handsfield, H.H., Genital herpes and public health: Addressing a global problem (2000) JAMA, 283, pp. 791-794 
504 |a Herold, B.C., Bourne, N., Marcellino, D., Kirkpatrick, R., Strauss, D.M., Zaneveld, L.J.D., Waller, D.P., Cooper, M.D., Poly(sodium 4-styrene sulfonate): An effective candidate topical antimicrobial for the prevention of sexually transmitted diseases (2000) J. Infect. Dis., 181, pp. 770-773 
504 |a Jennings, R., Clegg, A., The inhibitory effect of spermicidal agents on replication of HSV-2 and HIV-1 in vitro (1993) J. Antimicrob. Chemother., 32, pp. 71-82 
504 |a Keller, M.J., Klotman, M.E., Herold, B.C., Development of topical microbicides for prevention of human immunodeficiency virus and herpes simplex virus (2003) Am. J. Reprod. Immunol., 49, pp. 279-284 
504 |a Maguire, R.A., Bergman, N., Phillips, D.M., Comparison of microbicides for efficacy in protecting mice against vaginal challenge with herpes simplex virus type 2, cytotoxicity, antibacterial properties, and sperm immobilization (2001) Sex. Transm. Dis., 28, pp. 259-265 
504 |a Neyts, J., De Clercq, E., Effect of polyanionic compounds on intracutaneous and intravaginal herpesvirus infection in mice: Impact on the search for vaginal microbicides with anti-HIV activity (1995) J. Acquir. Immune Defic. Syndr. Hum. Retrovirol., 10, pp. 8-12 
504 |a Neyts, J., Snoeck, R., Schols, D., Balzarini, J., Esko, J.D., Van Schepdael, A., De Clercq, E., Sulfated polymers inhibit the interaction of human cytomegalovirus with cell surface heparan sulfate (1992) Virology, 189, pp. 48-58 
504 |a Parr, M.B., Kepple, L., McDermott, M.R., Drew, M.D., Bozzola, J.J., Parr, E.L., A mouse model for studies of mucosal immunity to vaginal infection by herpes simplex virus type 2 (1994) Lab. Invest., 70, pp. 369-380 
504 |a Piret, J., Lamontagne, J., Bestman-Smith, J., Roy, S., Gourde, P., Désormeaux, A., Omar, R.F., Bergeron, M.G., In vitro and in vivo evaluations of sodium lauryl sulfate and dextran sulfate as microbicides against herpes simplex and human immunodeficiency viruses (2000) J. Clin. Microbiol., 38, pp. 110-119 
504 |a Spieler Trager, R., Raising new barriers against HIV infection (2003) Science, 299, p. 39 
504 |a Stafford, M.K., Ward, H., Flanagan, A., Rosenstein, I.J., Taylor-Robinson, D., Smith, J.R., Weber, J., Kitchen, V.S., Safety study of nonoxynol-9 as a vaginal microbicide: Evidence of adverse effects (1998) J. Acquir. Immune Defic. Syndr. Hum. Retrovirol., 17, pp. 327-331 
504 |a Witvrouw, M., Desmyter, J., De Clercq, E., Antiviral portrait series: 4. Polysulfates as inhibitors of HIV and other enveloped viruses (1994) Antiviral Chem. Chemother., 5, pp. 345-359 
504 |a Zacharopoulos, V.R., Phillips, D.M., Vaginal formulations of carrageenan protect mice from herpes simplex virus infection (1997) Clin. Diagn. Lab. Immunol., 4, pp. 465-468 
504 |a Zeitlin, L., Whaley, K.J., Hegarty, T.A., Moench, T.R., Cone, R.A., Tests of vaginal microbicides in the mouse genital herpes model (1997) Contraception, 56, pp. 329-335 
520 3 |a In the present study, the protective effect of 1T1, a λ-carrageenan extracted from the red seaweed Gigartina skottsbergii was evaluated in a murine model of herpes simplex virus type 2 (HSV-2) genital infection. Six to eight-week-old female BALB/c mice were intravaginally inoculated with a lethal dose of HSV-2 (MS strain) and pre- or post-infection treated with different doses of a 10 mg/ml solution of 1T1. A single topical administration of 1T1 shortly before infection of BALB/c mice with HSV-2 protected 9 out of 10 mice from HSV-2-induced lesions and mortality, compared with only 10% survival in control mice. In addition, 1T1 produced a total blockade in virus shedding in the vaginal secretions. When 1T1 pre-treatment was reinforced with a second dose 2 h after infection, total protection was observed even when the prophylactic administration had taken place at 60 min before infection. The irreversible virucidal action of 1T1 against herpes virus seems to be responsible of its protective effect against virus replication and mortality following vaginal HSV-2 infection. © 2004 Elsevier B.V. All rights reserved.  |l eng 
536 |a Detalles de la financiación: Adhesives and Sealant Council 
536 |a Detalles de la financiación: Agencia Nacional de Promoción Científica y Tecnológica 
536 |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas 
536 |a Detalles de la financiación: This work was supported by Agencia Nacional de Promoción Científica y Tecnológica and Universidad de Buenos Aires (Argentina). MJC, LAS, ASC, and EBD are members of Research Career from Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET, Argentina). 
593 |a Laboratorio de Virología, Depto. de Quím. Biol., Fac. Cie. Exact. Y Nat., Univ. B., Argentina 
593 |a Departamento de Bioquímica, Univ. Federal Do Paraná, Curitiba, Brazil, Brazil 
593 |a Depto. de Quím. Orgán., Fac. de Ciencias Exactas Y Naturales, Univ. Buenos Aires, Buenos Aires, A., Argentina 
690 1 0 |a HERPES SIMPLEX VIRUS TYPE 2 
690 1 0 |a NATURAL CARRAGEENAN 
690 1 0 |a SEXUALLY TRANSMITTED DISEASE 
690 1 0 |a VAGINAL INFECTION 
690 1 0 |a VIRUCIDAL ACTIVITY 
690 1 0 |a CARRAGEENAN 
690 1 0 |a ANIMAL EXPERIMENT 
690 1 0 |a ANIMAL MODEL 
690 1 0 |a ARTICLE 
690 1 0 |a CONTROLLED STUDY 
690 1 0 |a DOSE RESPONSE 
690 1 0 |a DRUG EFFECT 
690 1 0 |a DRUG ISOLATION 
690 1 0 |a DRUG MECHANISM 
690 1 0 |a FEMALE 
690 1 0 |a GENITAL HERPES 
690 1 0 |a HERPES SIMPLEX VIRUS 2 
690 1 0 |a INFECTION PREVENTION 
690 1 0 |a INOCULATION 
690 1 0 |a LETHAL DOSE 
690 1 0 |a MORTALITY 
690 1 0 |a MOUSE 
690 1 0 |a NONHUMAN 
690 1 0 |a PRIORITY JOURNAL 
690 1 0 |a SEAWEED 
690 1 0 |a SURVIVAL RATE 
690 1 0 |a TIME 
690 1 0 |a VAGINA SECRETION 
690 1 0 |a VIRUS REPLICATION 
690 1 0 |a VIRUS SHEDDING 
690 1 0 |a VIRUS STRAIN 
690 1 0 |a ANIMALS 
690 1 0 |a ANTIVIRAL AGENTS 
690 1 0 |a CARRAGEENAN 
690 1 0 |a CERCOPITHECUS AETHIOPS 
690 1 0 |a FEMALE 
690 1 0 |a HERPES GENITALIS 
690 1 0 |a HERPESVIRUS 2, HUMAN 
690 1 0 |a MICE 
690 1 0 |a MICE, INBRED BALB C 
690 1 0 |a VAGINAL DISEASES 
690 1 0 |a VERO CELLS 
690 1 0 |a VIRUS SHEDDING 
690 1 0 |a ALGAE 
690 1 0 |a GIGARTINA 
690 1 0 |a GIGARTINA SKOTTSBERGII 
690 1 0 |a HERPESVIRIDAE 
690 1 0 |a HUMAN HERPESVIRUS 2 
690 1 0 |a MURINAE 
690 1 0 |a SIMPLEXVIRUS 
650 1 7 |2 spines  |a VAGINA 
700 1 |a Scolaro, L.A. 
700 1 |a Noseda, M.D. 
700 1 |a Cerezo, A.S. 
700 1 |a Damonte, E.B. 
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