A high-throughput screening for phosphatases using specific substrates
A high-throughput screening was developed for the detection of phosphatase activity in bacterial colonies. Unlike other methods, the current procedure can be applied to any phosphatase because it uses physiological substrates and detects the compelled product of all phosphatase reactions, that is, o...
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| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
Academic Press Inc.
2005
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| Materias: | |
| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| LEADER | 09207caa a22009137a 4500 | ||
|---|---|---|---|
| 001 | PAPER-4162 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203341.0 | ||
| 008 | 190411s2005 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-14844303755 | |
| 024 | 7 | |2 cas |a fructose bisphosphatase, 9001-52-9; phosphatase, 9013-05-2 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a ANBCA | ||
| 100 | 1 | |a Senn, A.M. | |
| 245 | 1 | 2 | |a A high-throughput screening for phosphatases using specific substrates |
| 260 | |b Academic Press Inc. |c 2005 | ||
| 270 | 1 | 0 | |m Senn, A.M.; Instituto Leloir, Fac. de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, Patricias Argentinas 435, C1405BWE Buenos Aires, Argentina; email: asenn@leloir.org.ar |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Van Ommen Kloeke, F., Baty, A.M., Eastburn, C.C., Diwu, Z., Geesey, G.G., Novel method for screening bacterial colonies for phosphatase activity (1999) J. Microbiol. Methods, 38, pp. 25-31 | ||
| 504 | |a Boquet, P.L., Manoil, C., Beckwith, J., Use of TnphoA to detect genes for exported proteins in Escherichia coli: Identification of the plasmid-encoded gene for a periplasmic acid phosphatase (1987) J. Bacteriol., 169, pp. 1663-1669 | ||
| 504 | |a Pradel, E., Boquet, P.L., Acid phosphatases of Escherichia coli: Molecular cloning and analysis of agp, the structural gene for a periplasmic acid glucose phosphatase (1988) J. Bacteriol., 170, pp. 4916-4923 | ||
| 504 | |a Riccio, M.L., Rossolini, G.M., Lombardi, G., Chiesurin, A., Satta, G., Expression cloning of different bacterial phosphatase-encoding genes by histochemical screening of genomic libraries onto an indicator medium containing phenolphthalein diphosphate and methyl green (1997) J. Appl. Microbiol., 82, pp. 177-185 | ||
| 504 | |a Satta, G., Pompei, R., Grazi, G., Cornaglia, G., Phosphatase activity is a constant feature of all isolates of all major species of the family Enterobacteriaceae (1988) J. Clin. Microbiol., 26, pp. 2637-2641 | ||
| 504 | |a Manafi, M., Kneifel, W., Bascomb, S., Fluorogenic and chromogenic substrates used in bacterial diagnostics (1991) Microbiol. Rev., 55, pp. 335-348 | ||
| 504 | |a Cabib, E., Duran, A., Simple and sensitive procedure for screening yeast mutants that lyse at nonpermissive temperatures (1975) J. Bacteriol., 124, pp. 1604-1606 | ||
| 504 | |a Chaffin, D.O., Rubens, C.E., Blue/White screening of recombinant plasmids in gram-positive bacteria by interruption of alkaline phosphatase gene (phoZ) expression (1998) Gene, 219, pp. 91-99 | ||
| 504 | |a Thaller, M.C., Berlutti, F., Schippa, S., Selan, L., Rossolini, G.M., Bacterial acid phosphatase gene fusions useful as targets for cloning-dependent insertional inactivation (1998) Biotechnol. Prog., 14, pp. 241-247 | ||
| 504 | |a Satta, G., Pompei, R., Ingianni, A., The selective staining mechanism of phosphatase producing colonies in the diphosphatephenolphthalein-methyl green method for the detection of bacterial phosphatase activity (1984) Microbiologica, 7, pp. 159-170 | ||
| 504 | |a Satta, G., Grazi, G., Varaldo, P.E., Fontana, R., Detection of bacterial phosphatase activity by means of an original and simple test (1979) J. Clin. Pathol., 32, pp. 391-395 | ||
| 504 | |a Gee, K.R., Sun, W.C., Bhalgat, M.K., Upson, R.H., Klaubert, D.H., Latham, K.A., Haugland, R.P., Fluorogenic substrates based on fluorinated umbelliferones for continuous assays of phosphatases and beta-galactosidases (1999) Anal. Biochem., 273, pp. 41-48 | ||
| 504 | |a Guzman, L.M., Belin, D., Carson, M.J., Beckwith, J., Tight regulation, modulation, and high-level expression by vectors containing the arabinose pBAD promoter (1995) J. Bacteriol., 177, pp. 4121-4130 | ||
| 504 | |a Rodriguez-Suarez, R.J., Wolosiuk, R.A., High level expression in Escherichia coli: Purification and properties of chloroplast fructose-1,6-bisphosphatase from rapeseed (Brassica napus) leaves (1995) Photosynth. Res., 46, pp. 313-322 | ||
| 504 | |a Chen, P.S., Toribara, T.Y., Warner, H., Microdetermination of phosphorus (1956) Anal. Chem., 28, pp. 1756-1758 | ||
| 504 | |a Lin-Goerke, J.L., Robbins, D.J., Burczak, J.D., PCR-based random mutagenesis using manganese and reduced dNTP concentration (1997) BioTechniques, 23, pp. 409-412 | ||
| 504 | |a Sambrook, J., Fritsch, E.F., Maniatis, T., (1989) Molecular Cloning: A Laboratory Manual, , Cold Spring Harbor Laboratory Press Cold Spring Harbor, NY | ||
| 504 | |a Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J., Protein measurement with the Folin phenol reagent (1951) J. Biol. Chem., 193, pp. 265-275 | ||
| 504 | |a Wolosiuk, R.A., Ballícora, M.A., Hagelin, K., The reductive pentose phosphate cycle for photosynthetic CO2 assimilation: Enzyme modulation (1993) FASEB J., pp. 622-637 | ||
| 504 | |a Fiske, C.H., Subbarow, Y., The colorimetric determination of phosphorus (1925) J. Biol. Chem., 66, pp. 375-400 | ||
| 504 | |a Chifflet, S., Torriglia, A., Chiesa, R., Tolosa, S., A method for the determination of inorganic phosphate in the presence of labile organic phosphate and high concentrations of protein: Application to lens ATPases (1988) Anal. Biochem., 168, pp. 1-4 | ||
| 504 | |a Cariani, L., Thomas, L., Brito, J., Del Castillo, J.R., Bismuth citrate in the quantification of inorganic phosphate and its utility in the determination of membrane-bound phosphatases (2004) Anal. Biochem., 324, pp. 79-83 | ||
| 504 | |a Wahler, D., Reymond, J.-L., High-throughput screening for biocatalysts (2001) Curr. Opin. Biotechnol., 12, pp. 535-544 | ||
| 504 | |a Goddard, J.-P., Reymond, J.-L., Enzyme assays for high-throughput screening (2004) Curr. Opin. Biotechnol., 15, pp. 314-322 | ||
| 520 | 3 | |a A high-throughput screening was developed for the detection of phosphatase activity in bacterial colonies. Unlike other methods, the current procedure can be applied to any phosphatase because it uses physiological substrates and detects the compelled product of all phosphatase reactions, that is, orthophosphate. In this method, substrates diffuse from a filter paper across a nitrocellulose membrane to bacterial colonies situated on the opposite face, and then reaction products flow back to the paper. Finally, a colorimetric reagent discloses the presence of orthophosphate in the filter paper. We validated the performance of this assay with several substrates and experimental conditions and with different phosphatases, including a library of randomly mutagenized rapeseed chloroplast fructose-1,6-bisphosphatase. This procedure could be extended to other enzymatic activities provided that an appropriate detection of reaction products is available. © 2004 Elsevier Inc. All rights reserved. |l eng | |
| 536 | |a Detalles de la financiación: Agencia Nacional de Promoción Científica y Tecnológica | ||
| 536 | |a Detalles de la financiación: Universidad de Buenos Aires | ||
| 536 | |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas | ||
| 536 | |a Detalles de la financiación: We are indebted to M. Berkmen and J. Beckwith (Harvard Medical School) for the generous gift of MB260 (agp) and MB65 (phoA) E. coli strains. This work was supported by a grant from the Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT) and the Universidad de Buenos Aires. Alejandro Senn holds a fellowship and Ricardo Wolosiuk is an investigator of the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). | ||
| 593 | |a Instituto Leloir, Fac. de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, Patricias Argentinas 435, C1405BWE Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a ENZYME ACTIVITY |
| 690 | 1 | 0 | |a HIGH-THROUGHPUT SCREENING |
| 690 | 1 | 0 | |a PHOSPHATASES |
| 690 | 1 | 0 | |a TRANSFORMED BACTERIA |
| 690 | 1 | 0 | |a FRUCTOSE BISPHOSPHATASE |
| 690 | 1 | 0 | |a PHOSPHATASE |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a BACTERIUM COLONY |
| 690 | 1 | 0 | |a BIOFILTER |
| 690 | 1 | 0 | |a CONTROLLED STUDY |
| 690 | 1 | 0 | |a ENZYME ACTIVITY |
| 690 | 1 | 0 | |a ENZYME MECHANISM |
| 690 | 1 | 0 | |a ENZYME SPECIFICITY |
| 690 | 1 | 0 | |a HIGH THROUGHPUT SCREENING |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a PHYSIOLOGY |
| 690 | 1 | 0 | |a PRIORITY JOURNAL |
| 690 | 1 | 0 | |a RAPESEED |
| 690 | 1 | 0 | |a BACTERIA (MICROORGANISMS) |
| 690 | 1 | 0 | |a SINAPIS ARVENSIS |
| 650 | 1 | 7 | |2 spines |a MUTAGENESIS |
| 700 | 1 | |a Wolosiuk, R.A. | |
| 773 | 0 | |d Academic Press Inc., 2005 |g v. 339 |h pp. 150-156 |k n. 1 |p Anal. Biochem. |x 00032697 |w (AR-BaUEN)CENRE-163 |t Analytical Biochemistry | |
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