Production of the main surface antigen of Toxoplasma gondii in tobacco leaves and analysis of its antigenicity and immunogenicity
We adapted a previously described Agrobacterium-mediated transient expression system to test the expression level of three constructs carrying the surface antigen 1 (SAG1) of Toxoplasma gondii. Two constructs were based in a Potato virus X (PVX) amplicon. In one of them, the PVX movement protein gen...
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2005
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| LEADER | 15444caa a22019577a 4500 | ||
|---|---|---|---|
| 001 | PAPER-4138 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230607131849.0 | ||
| 008 | 190411s2005 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-26244446912 | |
| 024 | 7 | |2 cas |a Antibodies, Protozoan; Antigens, Protozoan; Immunoglobulin G; Protozoan Proteins; SAG1 antigen, Toxoplasma | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 100 | 1 | |a Clemente, M. | |
| 245 | 1 | 0 | |a Production of the main surface antigen of Toxoplasma gondii in tobacco leaves and analysis of its antigenicity and immunogenicity |
| 260 | |c 2005 | ||
| 270 | 1 | 0 | |m Clemente, M.; IIB-INTECH, B7130IIWA, Camino Circunvalacion Laguna Km. 6, Chascomus, Provincia de Buenos Aires, Argentina; email: mclemente@intech.gov.ar |
| 506 | |2 openaire |e Política editorial | ||
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| 504 | |a Nigro, M., Gutierrez, A., Hoffer, A.M., Clemente, M., Kanfer, F., Carral, L., Evaluation of Toxoplasma gondii recombinant proteins for the diagnosis of recently acquired toxoplasmosis by an immunoglobulin G analysis (2003) Diagn. Microbiol. Infect. Dis., 47, pp. 609-613 | ||
| 504 | |a Mallory, A.C., Parks, G., Endres, M.W., Baulcombe, D., Bowman, L.H., Pruss, G.J., Vance, V.B., The amplicon-plus system for high-level expression of transgenes in plants (2002) Nat. Biothecnol., 20, pp. 622-625 | ||
| 504 | |a Brigneti, G., Voinnet, O., Li, W.X., Ji, L., Ding, S.W., Baulcombe, D., Viral pathogenicity determinant are suppressors of transgene silencing in Nicotiana benthamiana (1998) EMBO J., 17, pp. 6739-6746 | ||
| 504 | |a Harning, D., Spenter, J., Metsis, A., Vuust, J., Petersen, E., Recombinant Toxoplasma gondii surface Antigen 1 (P30) expressed in Escherichia coli is recognized by human Toxoplasma-specific immunoglobulin M (IgM) and IgG antibodies (1996) Clin. Diagn. Lab. Immunol., 3, pp. 355-357 | ||
| 504 | |a Chen, X.G., Gong, Y.H.U., Lun, Z.R., Fung, M.C., High- level expression and purification of immunogenic recombinant SAG1 (p30) of Toxoplasma gondii in Escherichia coli (2001) Protein Expr. Purif., 23, pp. 33-37 | ||
| 504 | |a Letourner, O., Gervasi, G., Gaia, S., Pages, J., Watelet, B., Jolivet, M., Characterization of Toxoplasma gondii surface, antigen 1 (SAG1) secreted from Pichia pastoris: Evidence of hyper O-glycosylation (2001) Biotechnol. Appl. Biochem., 33, pp. 35-45 | ||
| 504 | |a Marti, M., Li, Y., Kohler, P., Hehl, A.B., Conformationally correct expression of membrane-anchored Toxoplasma gondii SAG1 in the primitive protozoan Giardia duodenalis (2002) Infect. Immunol., 70, pp. 1014-1016 | ||
| 504 | |a Kim, K., Bulow, R., Kampmeier, J., Boothroyd, J.C., Conformationally appropriate expression of the Toxoplasma antigen SAG1 (p30) in CHO cells (1994) Infect. Immunol., 62, pp. 203-209 | ||
| 504 | |a Meek, B., Diepersloot, R.J., Van Gool, T., Speijer, D., Peek, R., IgM recognition of recombinant Toxoplasma gondii antigens by sera of acutely or latently infected humans (2003) Diagn. Microbiol. Infect. Dis., 45, pp. 45-52 | ||
| 504 | |a Biemans, R., Gregoire, D., Haumont, M., Bosseloir, A., Garcia, L., Jacquet, L., The conformation of purified Toxoplasma gondii SAG1 antigen, secreted from engineered Pichia pastoris, is adequate for serorecognition and cell proliferation (1998) J. Biotechnol., 66, pp. 137-146 | ||
| 504 | |a Vaquero, C., Sack, M., Chandler, J., Drossard, J., Schuster, F., Moneck, M., A carcinoembryonic antigen-specific antibody produced in tobacco (1999) Proc. Natl. Acad. Sci. USA, 96, pp. 11128-11133 | ||
| 504 | |a Desolme, B., Mévélec, M.N., Buzoni-Gatel, D., Bout, D., Induction of protective immunity against toxoplasmosis in mice by DNA immunization with a plasmid encoding Toxoplasma gondii Gra4 gene (2000) Vaccine, 18, pp. 2512-2521 | ||
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| 504 | |a Martin, V., Supanitsky, A., Echeverria, P.C., Litwin, S., Tanos, T., De Roodt, A.R., Recombinant Gra4 or Rop2 combined with alum or gra4 DNAa provides partial protection in chronic murine models of toxoplamosis (2004) Clin. Diagn. Lab. Immunol., 11, pp. 704-710 | ||
| 520 | 3 | |a We adapted a previously described Agrobacterium-mediated transient expression system to test the expression level of three constructs carrying the surface antigen 1 (SAG1) of Toxoplasma gondii. Two constructs were based in a Potato virus X (PVX) amplicon. In one of them, the PVX movement protein genes were replaced by the sag1 gene. In the other, the sag1 gene was placed under the control of an additional coat protein subgenomic promoter. In the third construct, the sag1 gene was fused to an apoplastic peptide signal under the CaMV 35S promoter. Western blot analysis of leaf extracts infiltrated with each construct revealed a protein of 35 kDa. SAG1 accumulation in leaves ranged from 0.1 to 0.06% of total soluble protein (equivalent to 10 μg and 6 μg of SAG1 per gram of fresh leaf tissue, respectively). Three of five human seropositive samples reacted with tobacco-expressed SAG1 in Western blot analysis. The C3H mice were immunized with SAG-expressing leaf extracts and perorally challenged with a nonlethal dose of the T. gondii Me49 strain. Mice vaccinated with SAG1 showed significantly lower brain cyst burdens compared to those from the control group. Immunization with SAG1-expressing leaves elicited a specific humoral response with predominant participation of type IgG2a. In conclusion, a functional SAG1 version could be transiently expressed in tobacco leaves. © 2005 Humana Press Inc. All rights of any nature whatsoever reserved. |l eng | |
| 536 | |a Detalles de la financiación: National Science and Technology Development Agency | ||
| 536 | |a Detalles de la financiación: Agencia Nacional de Promoción Científica y Tecnológica | ||
| 536 | |a Detalles de la financiación: M. Clemente, A. Zelada, S. O. Angel, and A. N. Mentaberry are Researchers of the National Research Council (CONICET, Argentina). R. Curilovic was a fellow of the University of Buenos Aires. A. Zelada and A. N. Mentaberry are also professors at the University of Buenos Aires (Departamento de Fisiología, Biología Molecular y Celular, FCEyN). M. Clemente and S. O. Angel are also professors of the National University of San Martín. This work was supported by PID 269/03 and PICT 05-11266 of the National Agency for Promotion of Science and Technology (ANPCyT, Argentina). | ||
| 593 | |a IIB-INTECH, B7130IIWA, Camino Circunvalacion Laguna Km. 6, Chascomus, Provincia de Buenos Aires, Argentina | ||
| 593 | |a INGEBI-CONICET Y FCEN-UBA, Vuelta de Obligado 2490, Buenos Aires, 1428, Argentina | ||
| 593 | |a IIB-INTECH, CONICET-UNSAM, B7130IIWA, Camino Circunvalacion Laguna Km. 6, Chascomús, Prov. Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a PLANT TRANSIENT EXPRESSION |
| 690 | 1 | 0 | |a PVX AMPLICON |
| 690 | 1 | 0 | |a TOXOPLASMA GONDII |
| 690 | 1 | 0 | |a BACTERIA |
| 690 | 1 | 0 | |a DISEASES |
| 690 | 1 | 0 | |a IMMUNOLOGY |
| 690 | 1 | 0 | |a PLANTS (BOTANY) |
| 690 | 1 | 0 | |a TOBACCO |
| 690 | 1 | 0 | |a VIRUSES |
| 690 | 1 | 0 | |a PLANT TRANSIENT EXPRESSION |
| 690 | 1 | 0 | |a PVX AMPLICON |
| 690 | 1 | 0 | |a SUBGENOMIC PROMOTERS |
| 690 | 1 | 0 | |a TOXOPLASMA GONDII |
| 690 | 1 | 0 | |a ANTIGENS |
| 690 | 1 | 0 | |a COAT PROTEIN |
| 690 | 1 | 0 | |a IMMUNOGLOBULIN G2A |
| 690 | 1 | 0 | |a MEMBRANE ANTIGEN |
| 690 | 1 | 0 | |a PLANT EXTRACT |
| 690 | 1 | 0 | |a SIGNAL PEPTIDE |
| 690 | 1 | 0 | |a SURFACE ANTIGEN 1 |
| 690 | 1 | 0 | |a TOXOPLASMA GONDII VACCINE |
| 690 | 1 | 0 | |a UNCLASSIFIED DRUG |
| 690 | 1 | 0 | |a VIRUS VECTOR |
| 690 | 1 | 0 | |a AMPLICON |
| 690 | 1 | 0 | |a ANIMAL EXPERIMENT |
| 690 | 1 | 0 | |a ANIMAL MODEL |
| 690 | 1 | 0 | |a ANIMAL TISSUE |
| 690 | 1 | 0 | |a ANTIGEN EXPRESSION |
| 690 | 1 | 0 | |a ANTIGEN SPECIFICITY |
| 690 | 1 | 0 | |a APOPLAST |
| 690 | 1 | 0 | |a BLOOD SAMPLING |
| 690 | 1 | 0 | |a BODY BURDEN |
| 690 | 1 | 0 | |a BRAIN CYST |
| 690 | 1 | 0 | |a CONTROLLED STUDY |
| 690 | 1 | 0 | |a FEMALE |
| 690 | 1 | 0 | |a FUNCTIONAL GENOMICS |
| 690 | 1 | 0 | |a GENE CONSTRUCT |
| 690 | 1 | 0 | |a GENE EXPRESSION REGULATION |
| 690 | 1 | 0 | |a GENE EXPRESSION SYSTEM |
| 690 | 1 | 0 | |a GENE FUSION |
| 690 | 1 | 0 | |a GENETIC STRAIN |
| 690 | 1 | 0 | |a HETEROZYGOTE |
| 690 | 1 | 0 | |a HUMAN |
| 690 | 1 | 0 | |a HUMORAL IMMUNITY |
| 690 | 1 | 0 | |a IMMUNIZATION |
| 690 | 1 | 0 | |a IMMUNOGENICITY |
| 690 | 1 | 0 | |a LETHALITY |
| 690 | 1 | 0 | |a MOTION |
| 690 | 1 | 0 | |a MOUSE |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a PLANT LEAF |
| 690 | 1 | 0 | |a PLANT TISSUE |
| 690 | 1 | 0 | |a PLANT VIRUS |
| 690 | 1 | 0 | |a POTATO VIRUS X |
| 690 | 1 | 0 | |a PROMOTER REGION |
| 690 | 1 | 0 | |a PROVOCATION TEST |
| 690 | 1 | 0 | |a REVIEW |
| 690 | 1 | 0 | |a RHIZOBIUM |
| 690 | 1 | 0 | |a SOLUBILITY |
| 690 | 1 | 0 | |a TOBACCO |
| 690 | 1 | 0 | |a TOXOPLASMA GONDII |
| 690 | 1 | 0 | |a VACCINATION |
| 690 | 1 | 0 | |a VIRAL GENE DELIVERY SYSTEM |
| 690 | 1 | 0 | |a WESTERN BLOTTING |
| 690 | 1 | 0 | |a ANIMALS |
| 690 | 1 | 0 | |a ANTIBODIES, PROTOZOAN |
| 690 | 1 | 0 | |a ANTIGENS, PROTOZOAN |
| 690 | 1 | 0 | |a BRAIN |
| 690 | 1 | 0 | |a CYSTS |
| 690 | 1 | 0 | |a FEMALE |
| 690 | 1 | 0 | |a GENE EXPRESSION |
| 690 | 1 | 0 | |a IMMUNOGLOBULIN G |
| 690 | 1 | 0 | |a MICE |
| 690 | 1 | 0 | |a MICE, INBRED C3H |
| 690 | 1 | 0 | |a PLANT LEAVES |
| 690 | 1 | 0 | |a POTEXVIRUS |
| 690 | 1 | 0 | |a PROTOZOAN PROTEINS |
| 690 | 1 | 0 | |a RHIZOBIUM |
| 690 | 1 | 0 | |a TOBACCO |
| 690 | 1 | 0 | |a TOXOPLASMA |
| 690 | 1 | 0 | |a TOXOPLASMOSIS |
| 690 | 1 | 0 | |a BACTERIA |
| 690 | 1 | 0 | |a DISEASES |
| 690 | 1 | 0 | |a PLANTS |
| 690 | 1 | 0 | |a TOBACCO |
| 690 | 1 | 0 | |a VIRUSES |
| 690 | 1 | 0 | |a AGROBACTERIUM |
| 690 | 1 | 0 | |a NICOTIANA TABACUM |
| 690 | 1 | 0 | |a POTATO VIRUS X |
| 690 | 1 | 0 | |a TOXOPLASMA GONDII |
| 650 | 1 | 7 | |2 spines |a GENES |
| 700 | 1 | |a Curilovic, R. | |
| 700 | 1 | |a Sassone, A. | |
| 700 | 1 | |a Zelada, A. | |
| 700 | 1 | |a Angel, S.O. | |
| 700 | 1 | |a Mentaberry, A.N. | |
| 773 | 0 | |d 2005 |g v. 30 |h pp. 41-49 |k n. 1 |p Mol. Biotechnol. |x 10736085 |t Molecular Biotechnology | |
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