Testosterone modulates Cav2.2 calcium channels' functional expression at rat levator ani neuromuscular junction
Spinal nucleus of bulbocavernosus and its target musculature, the bulbocavernosus and levator ani muscles, are sexually dimorphic, and their sexual differentiation depends on plasmatic levels of testosterone. Electrophysiological and immunocytochemical studies have demonstrated that at mammalian adu...
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2005
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| LEADER | 16873caa a22016697a 4500 | ||
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| 001 | PAPER-3986 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203330.0 | ||
| 008 | 190411s2005 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-23644443103 | |
| 024 | 7 | |2 cas |a testosterone, 58-22-0; Calcium Channel Blockers; Calcium Channels, L-Type; Calcium Channels, N-Type; Cav2.2 protein, rat; omega-Agatoxin IVA; omega-Conotoxin GVIA, 92078-76-7; Receptors, Cholinergic; Testosterone, 58-22-0 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a NRSCD | ||
| 100 | 1 | |a Nudler, S.I. | |
| 245 | 1 | 0 | |a Testosterone modulates Cav2.2 calcium channels' functional expression at rat levator ani neuromuscular junction |
| 260 | |c 2005 | ||
| 270 | 1 | 0 | |m Uchitel, O.D.; Departamento de Fisiología, Biología Molecular Y Celular, Facultad de Ciencias Exactas Y Naturales, Pabellón II-2do Piso, Buenos Aires, 1428, Argentina; email: odu@fbmc.fcen.uba.ar |
| 506 | |2 openaire |e Política editorial | ||
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| 520 | 3 | |a Spinal nucleus of bulbocavernosus and its target musculature, the bulbocavernosus and levator ani muscles, are sexually dimorphic, and their sexual differentiation depends on plasmatic levels of testosterone. Electrophysiological and immunocytochemical studies have demonstrated that at mammalian adult neuromuscular junctions only P/Q-type Ca2+ channels (Cav2.1), mediate evoked transmitter release. Here we report that N-type Ca2+ channel (Cav2.2) blocker ω-Conotoxin GVIA, as well as Cav2.1 blocker ω-Agatoxin IVA, significantly reduced quantal content of transmitter release by ∼ 80% and ∼70% respectively at levator ani muscle of the adult rats, indicating that neuromuscular transmission is jointly mediated by both types of channels. In these synapses, we also observed that castration and restitution of plasmatic testosterone in rats resulted in changes in the sensitivity to ω-Conotoxin GVIA. Castration induced, whereas testosterone treatment avoided, functional loss of Cav2.2, as mediators of transmitter release in these synapses. Strikingly, the expression and localization of α1B subunits, which form the pore of the Cav2.2 channel, were similar at control, gonadectomized and gonadectomized testosterone-treated rats, suggesting that testosterone may regulate the coupling mechanisms between Ca v2.2 and transmitter release at the neuromuscular junctions of these sexually dimorphic motoneurons. © 2005 IBRO. Published by Elsevier Ltd. All rights reserved. |l eng | |
| 593 | |a Departamento de Fisiología, Biología Molecular Y Celular, Facultad de Ciencias Exactas Y Naturales, Pabellón II-2do Piso, Buenos Aires, 1428, Argentina | ||
| 593 | |a Department of General Medical Sciences, Case Western Reserve University, School of Medicine, Cleveland, OH 44106, United States | ||
| 593 | |a Instituto de Neurociencias, Universidad Miguel Hernández, CSIC Campus de San Juan, 03550 San Juan de Alicante, Spain | ||
| 690 | 1 | 0 | |a Ω-CONOTOXIN GVIA |
| 690 | 1 | 0 | |a BULBOCAVERNOSUS |
| 690 | 1 | 0 | |a GONADECTOMY |
| 690 | 1 | 0 | |a IMMUNOSTAINING |
| 690 | 1 | 0 | |a SYNAPTIC TRANSMISSION |
| 690 | 1 | 0 | |a TESTOSTERONE PROPIONATE |
| 690 | 1 | 0 | |a CALCIUM CHANNEL |
| 690 | 1 | 0 | |a CALCIUM CHANNEL BLOCKING AGENT |
| 690 | 1 | 0 | |a CALCIUM CHANNEL N TYPE |
| 690 | 1 | 0 | |a CONOTOXIN |
| 690 | 1 | 0 | |a PROTEIN SUBUNIT |
| 690 | 1 | 0 | |a TESTOSTERONE |
| 690 | 1 | 0 | |a ANIMAL TISSUE |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a CASTRATION |
| 690 | 1 | 0 | |a CONTROLLED STUDY |
| 690 | 1 | 0 | |a GONADECTOMY |
| 690 | 1 | 0 | |a HORMONE ACTION |
| 690 | 1 | 0 | |a LEVATOR ANI MUSCLE |
| 690 | 1 | 0 | |a MALE |
| 690 | 1 | 0 | |a MOTONEURON |
| 690 | 1 | 0 | |a NEUROMUSCULAR SYNAPSE |
| 690 | 1 | 0 | |a NEUROMUSCULAR TRANSMISSION |
| 690 | 1 | 0 | |a NEUROTRANSMITTER RELEASE |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a PRIORITY JOURNAL |
| 690 | 1 | 0 | |a PROTEIN EXPRESSION |
| 690 | 1 | 0 | |a PROTEIN FUNCTION |
| 690 | 1 | 0 | |a PROTEIN LOCALIZATION |
| 690 | 1 | 0 | |a RAT |
| 690 | 1 | 0 | |a SENSITIVITY ANALYSIS |
| 690 | 1 | 0 | |a SYNAPSE |
| 690 | 1 | 0 | |a ANIMALS |
| 690 | 1 | 0 | |a ANIMALS, NEWBORN |
| 690 | 1 | 0 | |a CALCIUM CHANNEL BLOCKERS |
| 690 | 1 | 0 | |a CALCIUM CHANNELS, L-TYPE |
| 690 | 1 | 0 | |a CALCIUM CHANNELS, N-TYPE |
| 690 | 1 | 0 | |a DIAPHRAGM |
| 690 | 1 | 0 | |a DRUG INTERACTIONS |
| 690 | 1 | 0 | |a EVOKED POTENTIALS |
| 690 | 1 | 0 | |a GENE EXPRESSION REGULATION |
| 690 | 1 | 0 | |a IMMUNOHISTOCHEMISTRY |
| 690 | 1 | 0 | |a MALE |
| 690 | 1 | 0 | |a NEUROMUSCULAR JUNCTION |
| 690 | 1 | 0 | |a OMEGA-AGATOXIN IVA |
| 690 | 1 | 0 | |a OMEGA-CONOTOXIN GVIA |
| 690 | 1 | 0 | |a ORCHIECTOMY |
| 690 | 1 | 0 | |a PELVIC FLOOR |
| 690 | 1 | 0 | |a RADIOIMMUNOASSAY |
| 690 | 1 | 0 | |a RATS |
| 690 | 1 | 0 | |a RATS, SPRAGUE-DAWLEY |
| 690 | 1 | 0 | |a RECEPTORS, CHOLINERGIC |
| 690 | 1 | 0 | |a TESTOSTERONE |
| 700 | 1 | |a Pagani, M.R. | |
| 700 | 1 | |a Urbano, F.J. | |
| 700 | 1 | |a McEnery, M.W. | |
| 700 | 1 | |a Uchitel, O.D. | |
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