Identification and characterisation of the α and β subunits of succinyl CoA ligase of tomato
Despite the central importance of the TCA cycle in plant metabolism not all of the genes encoding its constituent enzymes have been functionally identified. In yeast, the heterodimeric protein succinyl CoA ligase is encoded for by two single-copy genes. Here we report the isolation of two tomato cDN...
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2005
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| 003 | AR-BaUEN | ||
| 005 | 20230518203321.0 | ||
| 008 | 190411s2005 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-27644511971 | |
| 024 | 7 | |2 cas |a succinic acid, 110-15-6; succinyl coenzyme A synthetase, 37341-57-4, 9014-36-2, 9080-33-5; Protein Subunits; Succinate-CoA Ligases, EC 6.2.1.-; Succinic Acid, 110-15-6 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a PMBID | ||
| 100 | 1 | |a Studart-Guimarães, C. | |
| 245 | 1 | 0 | |a Identification and characterisation of the α and β subunits of succinyl CoA ligase of tomato |
| 260 | |c 2005 | ||
| 270 | 1 | 0 | |m Fernie, A.R.; Max-Planck Institute for Molecular Plant Physiology, Am Mühlenberg 1, 14476 Golm, Germany; email: fernie@mpimp-golm.mpg.de |
| 506 | |2 openaire |e Política editorial | ||
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| 520 | 3 | |a Despite the central importance of the TCA cycle in plant metabolism not all of the genes encoding its constituent enzymes have been functionally identified. In yeast, the heterodimeric protein succinyl CoA ligase is encoded for by two single-copy genes. Here we report the isolation of two tomato cDNAs coding for α- and one coding for the β-subunit of succinyl CoA ligase. These three cDNAs were used to complement the respective Saccharomyces cerevisiae mutants deficient in the α- and β-subunit, demonstrating that they encode functionally active polypeptides. The genes encoding for the subunits were expressed in all tissues, but most strongly in floral and leaf tissues, with equivalent expression of the two α-subunit genes being expressed to equivalent levels in all tissues. In all instances GFP fusion expression studies confirmed an expected mitochondrial location of the proteins encoded. Following the development of a novel assay to measure succinyl CoA ligase activity, in the direction of succinate formation, the evaluation of the maximal catalytic activities of the enzyme in a range of tissues revealed that these paralleled those of mRNA levels. We also utilized this assay to perform a preliminary characterisation of the regulatory properties of the enzyme suggesting allosteric control of this enzyme which may regulate flux through the TCA cycle in a manner consistent with its position therein. © Springer 2005. |l eng | |
| 536 | |a Detalles de la financiación: We thank Lothar Willmitzer and members of AG Kramer for support and discussion during the course of this work and would like to acknowledge the financial support of the KAS (Konrad Adenauer Stiftung) to CSG. We thank Amit Gur and Dani Zamir (Hebrew University of Jerusalem, Rehovot, Israel) for the information concerning the map position of the genes described in this report and Joachim Selbig (same institute) for help with protein structure predictions. FC is member of conicet. | ||
| 593 | |a Max-Planck Institute for Molecular Plant Physiology, Am Mühlenberg 1, 14476 Golm, Germany | ||
| 593 | |a Laboratorio de Fisiología Y Biología Molecular, Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, Argentina | ||
| 593 | |a CSIRO-Plant Industry, P.O. Box 2601, Canberra, ACT, Australia | ||
| 593 | |a CICV-INTA, Instituto de Biotecnología, Las Cabañas y Los Reseros, B1712 WAA, Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a MITOCHONDRIAL METABOLISM |
| 690 | 1 | 0 | |a SACCHAROMYCES CEREVISIAE |
| 690 | 1 | 0 | |a SOLANUM LYCOPERSICUM |
| 690 | 1 | 0 | |a SUCCINYL COA LIGASE |
| 690 | 1 | 0 | |a TRICARBOXYLIC ACID CYCLE |
| 690 | 1 | 0 | |a CROPS |
| 690 | 1 | 0 | |a CYTOLOGY |
| 690 | 1 | 0 | |a ENZYMES |
| 690 | 1 | 0 | |a GENETIC ENGINEERING |
| 690 | 1 | 0 | |a METABOLISM |
| 690 | 1 | 0 | |a YEAST |
| 690 | 1 | 0 | |a MITOCHONDRIAL METABOLISM |
| 690 | 1 | 0 | |a SOLANUM LICOPERSICUM |
| 690 | 1 | 0 | |a SUCCINYL COA LIGASE |
| 690 | 1 | 0 | |a TRICARBOXYLIC ACID CYCLE |
| 690 | 1 | 0 | |a ENZYME KINETICS |
| 690 | 1 | 0 | |a PROTEIN SUBUNIT |
| 690 | 1 | 0 | |a SUCCINIC ACID |
| 690 | 1 | 0 | |a SUCCINYL COENZYME A SYNTHETASE |
| 690 | 1 | 0 | |a AMINO ACID SEQUENCE |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a CHEMISTRY |
| 690 | 1 | 0 | |a CYTOLOGY |
| 690 | 1 | 0 | |a DNA SEQUENCE |
| 690 | 1 | 0 | |a ENZYMOLOGY |
| 690 | 1 | 0 | |a GENE EXPRESSION REGULATION |
| 690 | 1 | 0 | |a GENETIC COMPLEMENTATION |
| 690 | 1 | 0 | |a GENETICS |
| 690 | 1 | 0 | |a METABOLISM |
| 690 | 1 | 0 | |a MOLECULAR CLONING |
| 690 | 1 | 0 | |a PHYLOGENY |
| 690 | 1 | 0 | |a PROTEIN TERTIARY STRUCTURE |
| 690 | 1 | 0 | |a PROTEIN TRANSPORT |
| 690 | 1 | 0 | |a SEQUENCE HOMOLOGY |
| 690 | 1 | 0 | |a TOMATO |
| 690 | 1 | 0 | |a AMINO ACID SEQUENCE |
| 690 | 1 | 0 | |a CLONING, MOLECULAR |
| 690 | 1 | 0 | |a GENE EXPRESSION REGULATION, ENZYMOLOGIC |
| 690 | 1 | 0 | |a GENE EXPRESSION REGULATION, PLANT |
| 690 | 1 | 0 | |a GENETIC COMPLEMENTATION TEST |
| 690 | 1 | 0 | |a LYCOPERSICON ESCULENTUM |
| 690 | 1 | 0 | |a PHYLOGENY |
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| 690 | 1 | 0 | |a PROTEIN TRANSPORT |
| 690 | 1 | 0 | |a SEQUENCE ANALYSIS, DNA |
| 690 | 1 | 0 | |a SEQUENCE HOMOLOGY, AMINO ACID |
| 690 | 1 | 0 | |a SUCCINATE-COA LIGASES |
| 690 | 1 | 0 | |a SUCCINIC ACID |
| 690 | 1 | 0 | |a CYTOLOGY |
| 690 | 1 | 0 | |a ENZYMATIC ACTIVITY |
| 690 | 1 | 0 | |a ENZYMES |
| 690 | 1 | 0 | |a GENETIC ENGINEERING |
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| 690 | 1 | 0 | |a SACCHAROMYCES CEREVISIAE |
| 690 | 1 | 0 | |a TOMATOES |
| 690 | 1 | 0 | |a LYCOPERSICON ESCULENTUM |
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| 650 | 1 | 7 | |2 spines |a GENES |
| 650 | 1 | 7 | |2 spines |a GENES |
| 700 | 1 | |a Gibon, Y. | |
| 700 | 1 | |a Frankel, N. | |
| 700 | 1 | |a Wood, C.C. | |
| 700 | 1 | |a Zanor, M.I. | |
| 700 | 1 | |a Fernie, A.R. | |
| 700 | 1 | |a Carrari, F. | |
| 773 | 0 | |d 2005 |g v. 59 |h pp. 781-791 |k n. 5 |p Plant. Mol. Biol. |x 01674412 |w (AR-BaUEN)CENRE-3503 |t Plant Molecular Biology | |
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