Antiviral mode of action of a synthetic brassinosteroid against Junin virus replication
The antiviral mode of action of the synthetic brassinosteroid (22S,23S)-3β-bromo-5α,22,23-trihydroxystigmastan-6-one (6b) against Junin virus replication in Vero cells was investigated. Time-related experiments showed that 6b mainly affects an early event of virus growth cycle. Neither adsorption no...
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2005
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| LEADER | 09875caa a22012257a 4500 | ||
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| 001 | PAPER-3870 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203320.0 | ||
| 008 | 190411s2005 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-27644532739 | |
| 024 | 7 | |2 cas |a (22S,23S)-3beta-bromo-5alpha,22,23-trihydroxystigmastan-6-one; Antiviral Agents; Cholestanones; DNA, Complementary; RNA, Viral; Viral Proteins | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a ARSRD | ||
| 100 | 1 | |a Castilla, V. | |
| 245 | 1 | 0 | |a Antiviral mode of action of a synthetic brassinosteroid against Junin virus replication |
| 260 | |c 2005 | ||
| 270 | 1 | 0 | |m Castilla, V.; Laboratorio de Virología, Departamento de Química Biológica, Pabellón 2, 1428 Buenos Aires, Argentina; email: viviana@qb.fcen.uba.ar |
| 506 | |2 openaire |e Política editorial | ||
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| 504 | |a Comin, M.J., Maier, M.S., Roccatagliata, A.J., Pujol, C.A., Damonte, E.B., Evaluation of the antiviral activity of natural sulfated polyhydroxysteroids and their synthetic derivatives and analogs (1999) Steroids, 64, pp. 335-340 | ||
| 504 | |a Damonte, E.B., Coto, C.E., Treatment of arenavirus infections: From basic studies to the challege of antiviral therapy (2002) Adv. Virus Res., 58, pp. 125-155 | ||
| 504 | |a Ellenberg, P., Edreira, M., Lozano, M., Scolaro, L., Synthesis and expression of viral antigens in Vero cells persistently infected with Junin virus (2002) Arch. Virol., 147, pp. 1543-1557 | ||
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| 504 | |a Laemmli, U.K., Cleavage of structural proteins during the assembly of the head bacteriophage T4 (1970) Nature, 227, pp. 680-685 | ||
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| 504 | |a McCormick, J.B., King, I.J., Webb, P.A., Scribner, C.L., Craven, R.B., Johnson, K.M., Elliot, L.H., Belmont-Williams, R., Lassa fever. Effective therapy with ribavirin (1986) N. Engl. J. Med., 314, pp. 20-26 | ||
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| 504 | |a Peters, C.J., Human infection with arenaviruses in the Americas (2002) Arenaviruses I, pp. 65-74. , M.B.A. Oldstone Springer-Verlag New York | ||
| 504 | |a Romanowski, V., Genetic organization of Junin virus, the etiological agent of Argentine hemorrhagic fever (1993) The Arenaviridae, pp. 51-84. , M.S. Salvato Plenum Press New York | ||
| 504 | |a Talarico, L.B., Ramírez, J.A., Galagovsky, L.R., Wachsman, M.B., Structure-activity relationship studies in a set of new brassinosteroid derivatives assayed against herpes simplex virus type 1 and 2 in cell cultures (2002) Med. Chem. Res., 11, pp. 434-444 | ||
| 504 | |a Teme Centurión, O.M., Galagovsky, L.R., Alternative synthesis of 24 (S)-homoethylcastasterone from stigmasterol (1998) Anal. Assoc. Quim. Arg., 86, pp. 104-109 | ||
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| 504 | |a Wachsman, M.B., López, E.M.F., Ramírez, J.A., Galagovsky, L.R., Coto, C.E., Antiviral effect of brassinosteroids against herpes virus and arenaviruses (2000) Antivir. Chem. Chemother., 11, pp. 71-77 | ||
| 504 | |a Wachsman, M.B., Ramírez, J.A., Galagovsky, L.R., Coto, C.E., Antiviral activity of brassinosteroid derivatives against measles virus in cell cultures (2002) Antivir. Chem. Chemother., 13, pp. 61-66 | ||
| 520 | 3 | |a The antiviral mode of action of the synthetic brassinosteroid (22S,23S)-3β-bromo-5α,22,23-trihydroxystigmastan-6-one (6b) against Junin virus replication in Vero cells was investigated. Time-related experiments showed that 6b mainly affects an early event of virus growth cycle. Neither adsorption nor internalization of viral particles was the target of the inhibitory action. The analysis of the effect of 6b on viral RNA synthesis demonstrated that the presence of the compound adversely affects virus RNA replication by preventing the synthesis of full length antigenomic RNA. Although 6b was most effective the earlier it was added to the cells after infection with JV, a high level of inhibition of JV yield and fusion activity of newly synthesized viral glycoproteins was still detected when the compound was present during the last hours of infection. Therefore, we cannot rule out an inhibitory action of 6b on later events of JV replicative cycle. © 2005 Elsevier B.V. All rights reserved. |l eng | |
| 536 | |a Detalles de la financiación: Universidad de Buenos Aires | ||
| 536 | |a Detalles de la financiación: Umweltbundesamt, X 046 | ||
| 536 | |a Detalles de la financiación: National Research Council | ||
| 536 | |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas, PIP 02236 | ||
| 536 | |a Detalles de la financiación: We thank Dr. L.R. Galagovsky and J.A. Ramírez for providing the compound 6b . This work was supported by grants from University of Buenos Aires, UBA, X 046, and National Research Council, CONICET, PIP 02236. Dr. Celia E. Coto is member of the Scientific Career of CONICET, Argentina. | ||
| 593 | |a Laboratorio de Virología, Departamento de Química Biológica, Pabellón 2, 1428 Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a ANTIVIRAL ACTIVITY |
| 690 | 1 | 0 | |a ARENAVIRUS |
| 690 | 1 | 0 | |a BRASSINOSTEROID |
| 690 | 1 | 0 | |a HEMORRHAGIC FEVER VIRUS |
| 690 | 1 | 0 | |a 3BETA BROMO 5ALPHA,22,23 TRIHYDROXYSTIGMASTAN 6 ONE |
| 690 | 1 | 0 | |a BRASSINOSTEROID |
| 690 | 1 | 0 | |a GENOMIC RNA |
| 690 | 1 | 0 | |a UNCLASSIFIED DRUG |
| 690 | 1 | 0 | |a VIRUS GLYCOPROTEIN |
| 690 | 1 | 0 | |a ANIMAL CELL |
| 690 | 1 | 0 | |a ANTIVIRAL ACTIVITY |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a CONTROLLED STUDY |
| 690 | 1 | 0 | |a DRUG MECHANISM |
| 690 | 1 | 0 | |a DRUG SYNTHESIS |
| 690 | 1 | 0 | |a JUNIN VIRUS |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a PRIORITY JOURNAL |
| 690 | 1 | 0 | |a RNA SYNTHESIS |
| 690 | 1 | 0 | |a VERO CELL |
| 690 | 1 | 0 | |a VIROGENESIS |
| 690 | 1 | 0 | |a VIRUS PARTICLE |
| 690 | 1 | 0 | |a VIRUS REPLICATION |
| 690 | 1 | 0 | |a ANIMALS |
| 690 | 1 | 0 | |a ANTIVIRAL AGENTS |
| 690 | 1 | 0 | |a CELL FUSION |
| 690 | 1 | 0 | |a CELL LINE |
| 690 | 1 | 0 | |a CERCOPITHECUS AETHIOPS |
| 690 | 1 | 0 | |a CHOLESTANONES |
| 690 | 1 | 0 | |a CRICETINAE |
| 690 | 1 | 0 | |a DNA, COMPLEMENTARY |
| 690 | 1 | 0 | |a GIANT CELLS |
| 690 | 1 | 0 | |a IMMUNOPRECIPITATION |
| 690 | 1 | 0 | |a JUNIN VIRUS |
| 690 | 1 | 0 | |a REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION |
| 690 | 1 | 0 | |a RNA, VIRAL |
| 690 | 1 | 0 | |a VERO CELLS |
| 690 | 1 | 0 | |a VIRAL PROTEINS |
| 690 | 1 | 0 | |a VIRUS REPLICATION |
| 690 | 1 | 0 | |a ANIMALIA |
| 690 | 1 | 0 | |a ARENAVIRUS |
| 690 | 1 | 0 | |a JUNIN VIRUS |
| 650 | 1 | 7 | |2 spines |a VIRUS RNA |
| 700 | 1 | |a Larzábal, M. | |
| 700 | 1 | |a Sgalippa, N.A. | |
| 700 | 1 | |a Wachsman, M.B. | |
| 700 | 1 | |a Coto, C.E. | |
| 773 | 0 | |d 2005 |g v. 68 |h pp. 88-95 |k n. 2 |p Antiviral Res. |x 01663542 |w (AR-BaUEN)CENRE-3746 |t Antiviral Research | |
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| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_01663542_v68_n2_p88_Castilla |y Handle |
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