Molecular detection of dengue viruses in field caught Aedes aegypti mosquitoes from northeastern Argentina

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Most molecular protocols for Dengue virus detection described so far are time consuming and cumbersome with mosquito samples. In order to count with a sensitive and specific molecular detection system for monitoring possible Dengue outbreaks and circulating viral serotypes in field-caught Aedes aegy...

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Autor principal: Liotta, D.J
Otros Autores: Cabanne, Gustavo Sebastián, Campos, R., Tonon, S.A
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 2005
Materias:
ARGENTINA
Acceso en línea:Registro en Scopus
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Registro en la Biblioteca Digital
Aporte de:Registro referencial: Solicitar el recurso aquí
Biblioteca Central Dr. Luis F. Leloir (FCEN) de Universidad de Buenos Aires
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100 1 |a Liotta, D.J. 
245 1 0 |a Molecular detection of dengue viruses in field caught Aedes aegypti mosquitoes from northeastern Argentina 
260 |c 2005 
270 1 0 |m Liotta, D.J.; Laboratorio de Biología Molecular Aplicada, Facultad de Ciencias Exactas Químicas y Naturales, Universidad Nacional de Misiones, Avenida Mariano Moreno 1375, Posadas, 3300, Argentina; email: liotta@mbf.unam.edu.ar 
504 |a Acha, P., Szyfres, B., (1997) Zoonosis y Enfermedades Transmisibles Comunes Al Hombre y a Los Animales, pp. 302-305. , Pan American Health Organization (Editor), Washington D.C.; Scientific publication No. 503 
504 |a Avilés, G., Rangeón, G., Vorndam, V., Briones, A., Baroni, P., Enria, D., Sabattini, M.S., Dengue Reemergence in Argentina (1999) Emerg Infect Dis, 5 (4), pp. 575-578 
504 |a Avilés, G., Paz, V., Rangeón, G., Ranaivoarisoa, M., Verzeri, N., Roginski, S., Baroni, P., Enria, Laboratory Surveillance of Dengue in Argentina, 1995-2001 (2003) Emerg Infect Dis, 9 (6), pp. 738-742 
504 |a Boffi, R., Programa de prevención del Dengue y control del Aedes aegypti (1998) Temas de Zoonosis y Enfermedades Emergentes. II Congreso Argentino de Zoonosis, pp. 413-419. , I Congreso Argentino y Latinoamericano de Enfermedades Emergentes y Asociación Argentina de Zoonosis (Editors) 
504 |a Boom, R., Sol, C.J., Salimans, M.M., Jansen, C.L., Wertheim-Van Dillen, P.M., Van Der Noorda, J., Rapid and simple method for purification of nucleic acids (1990) J Clin Microbiol, 28, pp. 495-503 
504 |a Chow, V., Chan, Y., Yong, R., Lee, M., Lim, L., Chung, Y., Lam-Phua, S., Tan, B., Monitoring of Dengue viruses in field-caught Aedes aegypti and Aedes albopictus mosquitoes by a type-specific polimerase chain reaction and cycle sequencing (1998) Am J Trop Med Hyg, 58 (5), pp. 578-586 
504 |a Consoli, R., De Oliveira, R., (1994) Principais Mosquitos de Importancia Sanitaria No Brasil, pp. 104-117. , Fiocruz Editor, Brazil 
504 |a Deubel, V., Laille, V., Hugnot, J., Chunge, E., Guesdon, J., Drouet, M., Bassot, S., Chevrier, D., Identification of Dengue sequences by genomic amplification: Rapid diagnosis of Dengue Virus serotypes in peripheral blood (1990) J Virol Methods, 30, pp. 41-54 
504 |a Harris, E., Roberts, G., Smith, L., Selle, J., Kramer, L., Valle, S., Sandoval, E., Balmaseda, A., Typing of Dengue viruses in clinical specimens and mosquitoes by single-tube multiplex Reverse Transcriptase PCR (1998) J Clin Microbiol, 36, pp. 2634-2639 
504 |a Jensen, T., Comparison of Bi-directional Fay, Omni-directional, CDC, and duplex cone traps for sampling adult Aedes albopictus and Aedes aegypti in north Florida (1994) J Am Mosq Control Assoc, 10 (1), pp. 74-78 
504 |a Kourí, G., Guzmán, M.G., Valdés, L., Carbonel, I., Del Rosario, D., Vazquez, S., Laferté, J., Cabrera, M., Re-emergence of Dengue in Cuba: A 1997 Epidemic in Santiago de Cuba (1998) Emerg Infect Dis, 4 (1), pp. 89-92 
504 |a Kuno, G., Universal diagnostic RT-PCR protocol for Arboviruses (1998) J Virol Methods, 72, pp. 27-41 
504 |a Lanciotti, R.S., Calisher, C., Gubler, D., Chang, G., Vorndam, V., Rapid detection and typing of Dengue Viruses from clinical samples by using Reverse Transcriptase-Polymerase Chain Reaction (1992) J Clin Virol, 30, pp. 545-551 
504 |a León, T., (2001), Epidemiology Chief, National Ministery of Health, Republic of Paraguay. Personal communicationMartínez Torres, E., (1998) Dengue y Dengue Hemorrágico, pp. 43-56. , Universidad Nacional de Quilmes & Elea Laboratorio (Editors) 
504 |a Miagostovich, M.P., Nogueira, R.M., Schatzmayr, H., Lanciotti, R., Molecular epidemiology of Den-2 virus in Brazil (1998) Mem Inst Oswaldo Cruz, 93 (5), pp. 625-626 
504 |a (1994) Dengue and Dengue Haemorrhagic Fever: Guidelines for Prevention and Control, , Washington D.C. Scientific publication No. 548 
504 |a Peters, C.J., Viral hemorrhagic fevers (1997) Viral Pathogenesis, pp. 779-799. , Nathanson N. (Editor). Lippincott-Raven Publishers, Philadelphia 
504 |a Sabattini, M.S., Avilés, G., Monath, T.P., Historical, epidemiological, and ecological aspects of arboviruses in Argentina: Flaviviridae, Bunyaviridae and Rhabdoviridae (1998) An Overview of Arbovirology in Brazil and Neighboring Countries, pp. 113-134. , Travassos da Rosa, Vasconcelos, Travassos da Rosa (Editors). Belem, Brazil. Instituto Evandro Chagas 
504 |a Sudiro, T., Ishiko, H., Green, S., Vaughn, D., Nisalak, A., Kalayanarooj, S., Rothman, A., Rapid diagnosis of Dengue viremia by Reverse Transcriptase-Polymerase Chain Reaction using 3′-noncoding region universal primers (1997) Am J Trop Med Hyg, 56, pp. 424-429 
504 |a Tanaka, M., Rapid identification of Flavivirus using the Polymerase Chain Reaction (1993) J Virol Methods, 41, pp. 311-322 
504 |a Wallace, D.M., (1987) Methods in Enzymology, 152, pp. 33-41. , Berger and Kimmar (Editors), Academic Press. Molecular Cloning Techniques Series 
504 |a (1997) Dengue Haemorrhagic Fever: Diagnosis, Treatment, Prevention and Control. 2nd Edition, pp. 60-66. , Geneva 
506 |2 openaire  |e Política editorial 
520 3 |a Most molecular protocols for Dengue virus detection described so far are time consuming and cumbersome with mosquito samples. In order to count with a sensitive and specific molecular detection system for monitoring possible Dengue outbreaks and circulating viral serotypes in field-caught Aedes aegypti populations from Northeastern Argentina, a RT-PCR and RFLP assay was developed. The original RT-PCR assay proposed by Sudiro et al. for human serum was optimized for mosquito samples. Modifications were done at the RNA extraction-purification and at the thermal profile steps. The generic 230 bp amplicon was validated by RFLP assay and cycle sequencing. Results showed that, due to the generic characteristic of the primers used, certain mosquito genome regions could be co-amplified, making confirmation of the Dengue specific amplicon by RFLP assay a required step. Under these conditions, the proposed method can be employed as a Dengue viral generic screening procedure in Aedes aegypti mosquito samples, giving in our hands an estimated 99.52% of confirmed negatives (207/208 tested samples).  |l eng 
593 |a Laboratorio de Biología Molecular Aplicada, Facultad de Ciencias Exactas, Químicas y Naturales, Universidad Nacional de Misiones, Posadas, Argentina 
593 |a Cátedra de Virología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina 
593 |a Laboratorio de Biología Molecular Aplicada, Facultad de Ciencias Exactas Químicas y Naturales, Universidad Nacional de Misiones, Avenida Mariano Moreno 1375, Posadas, 3300, Argentina 
651 4 |a ARGENTINA 
651 4 |a ARGENTINA 
690 1 0 |a AEDES AEGYPTI 
690 1 0 |a DENGUE 
690 1 0 |a RFLP 
690 1 0 |a RNA 
690 1 0 |a RT-PCR 
690 1 0 |a PRIMER RNA 
690 1 0 |a AEDES AEGYPTI 
690 1 0 |a AMPLICON 
690 1 0 |a ARTICLE 
690 1 0 |a CONTROLLED STUDY 
690 1 0 |a DENGUE VIRUS 
690 1 0 |a GENE SEQUENCE 
690 1 0 |a MOSQUITO 
690 1 0 |a NONHUMAN 
690 1 0 |a RESTRICTION FRAGMENT LENGTH POLYMORPHISM 
690 1 0 |a REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION 
690 1 0 |a RNA EXTRACTION 
690 1 0 |a RNA PURIFICATION 
690 1 0 |a SCREENING 
690 1 0 |a SEROTYPING 
690 1 0 |a VIRAL GENETICS 
690 1 0 |a VIRUS DETECTION 
690 1 0 |a AEDES 
690 1 0 |a ANIMALS 
690 1 0 |a BASE SEQUENCE 
690 1 0 |a DENGUE 
690 1 0 |a DENGUE VIRUS 
690 1 0 |a FEMALE 
690 1 0 |a HUMANS 
690 1 0 |a INSECT VECTORS 
690 1 0 |a MOLECULAR SEQUENCE DATA 
690 1 0 |a POLYMORPHISM, RESTRICTION FRAGMENT LENGTH 
690 1 0 |a REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION 
690 1 0 |a SEQUENCE ALIGNMENT 
690 1 0 |a SEQUENCE HOMOLOGY, NUCLEIC ACID 
690 1 0 |a AEDES AEGYPTI 
690 1 0 |a DENGUE VIRUS 
690 1 0 |a DENGUE VIRUS GROUP 
700 1 |a Cabanne, Gustavo Sebastián 
700 1 |a Campos, R. 
700 1 |a Tonon, S.A. 
773 0 |d 2005  |g v. 47  |h pp. 82-87  |k n. 3-4  |p Rev. Latinoam. Microbiol.  |x 01874640  |t Revista Latinoamericana de Microbiologia 
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