ssp Genes and spore osmotolerance in Bacillus thuringiensis israelensis and Bacillus sphaericus

It was shown previously that spores and vegetative cells of Bacillus sphaericus (Bf) and Bacillus thuringiensis israelensis (Bti) are very sensitive to osmotic variations. Since spore osmotolerance has been associated with their SASP (small acid soluble spore proteins) content coded by ssp genes, hy...

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Autor principal: Cucchi, A.
Otros Autores: De Rivas, C.S
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: Springer-Verlag 1995
Acceso en línea:Registro en Scopus
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024 7 |2 scopus  |a 2-s2.0-0028851014 
024 7 |2 cas  |a Bacillus spore-specific proteins; Bacterial Proteins 
040 |a Scopus  |b spa  |c AR-BaUEN  |d AR-BaUEN 
030 |a CUMID 
100 1 |a Cucchi, A. 
245 1 0 |a ssp Genes and spore osmotolerance in Bacillus thuringiensis israelensis and Bacillus sphaericus 
260 |b Springer-Verlag  |c 1995 
270 1 0 |m Cucchi, A.; Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria Pabellón II 4cPiso (1428), Buenos Aires, Argentina 
506 |2 openaire  |e Política editorial 
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504 |a Loshon, C.A., Setlow, P., Levels of small molecules in dormant spores of Sporosarcina species and comparison with levels in spores of Bacillus and Clostridium species (1993) Can J Microbiol, 39, pp. 259-262 
504 |a Maniatis, T., Fritsch, E.F., Sambrook, J., (1982) Molecular cloning: a laboratory manual, , Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY 
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504 |a Mason, J.M., Hackett, R.H., Setlow, P., Regulation of expression of genes coding for small, acid-soluble proteins of Bacillus subtilis spores: studies using lacZ fusions (1988) J Bacteriol, 170, pp. 239-244 
504 |a Measures, J.C., Role of amino acids in osmoregulation of non-halophilic bacteria (1975) Nature, 257, pp. 398-400 
504 |a Porter, A.G., Davidson, E.W., Liu, J-W, Mosquitocidal toxins of bacilli and their genetic manipulation for effective biological control of mosquitoes (1993) Microbiol Rev, 57, pp. 838-861 
504 |a Priest, F.G., Biological control of mosquitoes and other biting flies by Bacillus sphaericus and Bacillus thuringiensis (1992) J Appl Bacteriol, 72, pp. 357-369 
504 |a Rao, H., Mohr, S.C., Fairhead, H., Setlow, P., Synthesis and characterization of a 29-amino acid residue DNA-binding peptide derived from alpha/beta-type small, acid-soluble spore proteins (SASP) of bacteria (1992) FEBS Lett, 305, pp. 115-120 
504 |a Ruzal, S.M., Sanchez Rivas, C., Physiological and genetic characterization of the osmotic stress response in Bacillus subtilis (1994) Can J Microbiol, 40, pp. 140-144 
504 |a Ruzal, S.M., Alice, A.F., Sanchez Rivas, C., Osmoresistance of spores from Bacillus subtilis and the effect of ssp mutations (1994) Microbiol, 140, pp. 2173-2177 
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504 |a Setlow, B., Hackett, R.H., Setlow, P., Noninvolvement of the spore cortex in acquisition of low-molecular-weight basic proteins and UV light resistance during Bacillus sphaericus sporulation (1982) J Bacteriol, 149, pp. 494-498 
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504 |a Thanabalu, T., Hindley, J., Brenner, S., Oei, C., Berry, C., Expression of the mosquitocidal toxins of Bacillus sphaericus and Bacillus thuringiensis subsp. israelensis by recombinant Caulobater crescentus, a vehicle for biological control of aquatic insect larvae (1992) Appl Environ Microbiol, 58, pp. 905-910 
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504 |a Yasbin, R., Fields, P., Anderson, B., Properties of Bacillus subtilis 168 derivatives free of their natural prophages (1980) Gene, 12, pp. 155-159 
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520 3 |a It was shown previously that spores and vegetative cells of Bacillus sphaericus (Bf) and Bacillus thuringiensis israelensis (Bti) are very sensitive to osmotic variations. Since spore osmotolerance has been associated with their SASP (small acid soluble spore proteins) content coded by ssp genes, hybridization assays were performed with sspE and sspA genes from B. subtilis as probes and showed that Bti and Bf strains could lack an sspE-like gene. The B. subtilis sspE gene was then introduced into Bti 4Q2 strain; spores were obtained and showed a 65 to 650 times higher level of osmotolerance to NaCl, without affecting other important properties: hypoosmotic resistance in vegetative cells, spore UV resistance, and larvicidal activity against diptera larvae. © 1995 Springer-Verlag New York Inc.  |l eng 
593 |a Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria Pabellón II 4cPiso (1428), Buenos Aires, Argentina 
690 1 0 |a ARTICLE 
690 1 0 |a BACILLUS SPHAERICUS 
690 1 0 |a BACILLUS THURINGIENSIS 
690 1 0 |a BACTERIAL GENE 
690 1 0 |a BACTERIAL SPORE 
690 1 0 |a HYBRIDIZATION 
690 1 0 |a NONHUMAN 
690 1 0 |a OSMOTIC STRESS 
690 1 0 |a PRIORITY JOURNAL 
690 1 0 |a SPOROGENESIS 
690 1 0 |a ULTRAVIOLET RADIATION 
690 1 0 |a ANIMAL 
690 1 0 |a BACILLUS THURINGIENSIS 
690 1 0 |a BACTERIAL PROTEINS 
690 1 0 |a DIPTERA 
690 1 0 |a GENES, BACTERIAL 
690 1 0 |a LARVA 
690 1 0 |a PEST CONTROL, BIOLOGICAL 
690 1 0 |a SPECIES SPECIFICITY 
690 1 0 |a SPORES, BACTERIAL 
690 1 0 |a SUPPORT, NON-U.S. GOV'T 
690 1 0 |a TRANSFECTION 
690 1 0 |a ULTRAVIOLET RAYS 
690 1 0 |a WATER-ELECTROLYTE BALANCE 
690 1 0 |a BACILLUS SPHAERICUS 
690 1 0 |a BACILLUS SUBTILIS 
690 1 0 |a BACILLUS THURINGIENSIS SEROVAR ISRAELENSIS 
690 1 0 |a BACTERIA (MICROORGANISMS) 
690 1 0 |a DIPTERA 
700 1 |a De Rivas, C.S. 
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