Regulation of phenobarbital-induced ferrochelatase mRNA activity by dibutyryl cAMP and glucose in normal and diabetic rat hepatocytes.

The induction of ferrochelatase activity by phenobarbital and its potentiation by dibutyryl cAMP assayed in normal rat hepatocytes are associated with increased activity of ferrochelatase mRNA. Glucose inhibits this stimulatory effect. This inhibition can be reversed with increasing concentrations o...

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Detalles Bibliográficos
Autor principal: Cánepa, E.T
Otros Autores: Pereda, M.P, Llambías, E.B, Grinstein, M.
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 1992
Acceso en línea:Registro en Scopus
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Registro en la Biblioteca Digital
Aporte de:Registro referencial: Solicitar el recurso aquí
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024 7 |2 scopus  |a 2-s2.0-0026451453 
024 7 |2 cas  |a bucladesine, 16980-89-5, 362-74-3; ferrochelatase, 9012-93-5; glucose, 50-99-7, 84778-64-3; phenobarbital, 50-06-6, 57-30-7, 8028-68-0; Bucladesine, 362-74-3; Ferrochelatase, EC 4.99.1.1; Glucose, 50-99-7; Phenobarbital, 50-06-6, 362-74-3; RNA, Messenger 
040 |a Scopus  |b spa  |c AR-BaUEN  |d AR-BaUEN 
100 1 |a Cánepa, E.T. 
245 1 0 |a Regulation of phenobarbital-induced ferrochelatase mRNA activity by dibutyryl cAMP and glucose in normal and diabetic rat hepatocytes. 
260 |c 1992 
270 1 0 |m Cánepa, E.T. 
506 |2 openaire  |e Política editorial 
520 3 |a The induction of ferrochelatase activity by phenobarbital and its potentiation by dibutyryl cAMP assayed in normal rat hepatocytes are associated with increased activity of ferrochelatase mRNA. Glucose inhibits this stimulatory effect. This inhibition can be reversed with increasing concentrations of dibutyryl cAMP. The inducing effect exerted by phenobarbital on the activity of ferrochelatase mRNA in diabetic hepatocytes is greater than that observed in normal cells. This enhanced response in diabetic rat hepatocytes is neither potentiated by adding dibutyryl cAMP nor repressed by glucose. The absence of a glucose effect persists even when the endogenous cAMP content is lowered to normal levels. The results obtained in this study are consistent with those reported in other published studies of ferrochelatase activity. This adds more experimental evidence to support the concept that ferrochelatase is inducible. The results obtained suggest that ferrochelatase is more susceptible to induction with phenobarbital in diabetic rat hepatocytes than in normal rat hepatocytes.  |l eng 
593 |a Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina 
690 1 0 |a BUCLADESINE 
690 1 0 |a FERROCHELATASE 
690 1 0 |a GLUCOSE 
690 1 0 |a MESSENGER RNA 
690 1 0 |a PHENOBARBITAL 
690 1 0 |a ANIMAL 
690 1 0 |a ARTICLE 
690 1 0 |a BIOSYNTHESIS 
690 1 0 |a DRUG EFFECT 
690 1 0 |a ENZYME INDUCTION 
690 1 0 |a EXPERIMENTAL DIABETES MELLITUS 
690 1 0 |a GENE EXPRESSION REGULATION 
690 1 0 |a LIVER 
690 1 0 |a MALE 
690 1 0 |a METABOLISM 
690 1 0 |a RAT 
690 1 0 |a RAT STRAIN 
690 1 0 |a ANIMALS 
690 1 0 |a BUCLADESINE 
690 1 0 |a DIABETES MELLITUS, EXPERIMENTAL 
690 1 0 |a ENZYME INDUCTION 
690 1 0 |a FERROCHELATASE 
690 1 0 |a GENE EXPRESSION REGULATION 
690 1 0 |a GLUCOSE 
690 1 0 |a LIVER 
690 1 0 |a MALE 
690 1 0 |a PHENOBARBITAL 
690 1 0 |a RATS 
690 1 0 |a RATS, INBRED STRAINS 
690 1 0 |a RNA, MESSENGER 
700 1 |a Pereda, M.P. 
700 1 |a Llambías, E.B. 
700 1 |a Grinstein, M. 
773 0 |d 1992  |g v. 70  |h pp. 26-33  |k n. 1  |p Biochem. Cell Biol.  |x 08298211  |w (AR-BaUEN)CENRE-3918  |t Biochemistry and cell biology = Biochimie et biologie cellulaire 
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856 4 0 |u https://doi.org/10.1139/o92-004  |y DOI 
856 4 0 |u https://hdl.handle.net/20.500.12110/paper_08298211_v70_n1_p26_Canepa  |y Handle 
856 4 0 |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08298211_v70_n1_p26_Canepa  |y Registro en la Biblioteca Digital 
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962 |a info:eu-repo/semantics/article  |a info:ar-repo/semantics/artículo  |b info:eu-repo/semantics/publishedVersion 
999 |c 61308