Stability study on renal type I mineralocorticoid receptor
The purpose of this work is to review stability and activation properties of type 1 receptor, in order to explain the reasons for its extreme in vitro instability. We demonstrate that the treatment of rat kidney cytosol with H2O2 prevents aldosterone binding, DNA/steroid- receptor complex interactio...
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| Autor principal: | |
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| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier Inc.
1996
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| LEADER | 07223caa a22010097a 4500 | ||
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| 001 | PAPER-3526 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203259.0 | ||
| 008 | 190411s1996 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0030581094 | |
| 024 | 7 | |2 cas |a 5,5' dithiobis(2 nitrobenzoic acid), 69-78-3; aldosterone, 52-39-1, 6251-69-0; DNA, 9007-49-2; edetic acid, 150-43-6, 60-00-4; egtazic acid, 67-42-5; hydrogen peroxide, 7722-84-1; iron, 14093-02-8, 53858-86-9, 7439-89-6; mercaptoethanol, 60-24-2; n ethylmaleimide, 128-53-0 | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 030 | |a LIFSA | ||
| 100 | 1 | |a Galigniana, M.D. | |
| 245 | 1 | 0 | |a Stability study on renal type I mineralocorticoid receptor |
| 260 | |b Elsevier Inc. |c 1996 | ||
| 270 | 1 | 0 | |m Galigniana, M.D.; Departamento de Quimica Biologica, FCEN, Universidad de Buenos Aires, Buenos Aires 1428, Argentina |
| 506 | |2 openaire |e Política editorial | ||
| 504 | |a Arriza, J., Weinberg, C., Cerelli, G., Glaser, T., Handelin, B., Housman, D., Evans, R., (1987) Science, 237, pp. 268-275 | ||
| 504 | |a Patel, P., Sherman, T., Goldman, D., Watson, S., (1989) Mol. Endocrinol., 3, pp. 1877-1885 | ||
| 504 | |a Ress, A., Bell, P., (1975) Biochem. Biophys. Acta, 411, pp. 121-132 | ||
| 504 | |a Bodwell, J., Halbrook, N., Munck, A., (1984) Biochemistry, 23, pp. 4237-4242 | ||
| 504 | |a Emadian, S., Luttge, W., Desmore, C., (1986) J. Steroid Biochem., 24, pp. 953-961 | ||
| 504 | |a Galigniana, M., Vicent, G., Lantos, C., Burton, G., (1993) An.Asoc.Quim.Argent., pp. 81333-81340 | ||
| 504 | |a Luttge, W., Gray, H., Densmore, C., (1984) J. Steroid Biochem., 20, pp. 545-553 | ||
| 504 | |a Binart, N., Lomes, M., Rafestin-Oblin, M., E.baulieu, E., (1991) Proc. Natl. Acad. Sci., 88, pp. 10681-10685 | ||
| 504 | |a Luttge, W., Emadian, S., (1988) Life Sci., 43, pp. 837-843 | ||
| 504 | |a Falks, J., (1964) Porphyrins and Metalloporphvrins. BBA Library, 185-186, Elsevier Publishing Co., New York | ||
| 504 | |a Furhop, J., Smith, K., Laboratory Methods in Porphvrins and Metalloporphvrins Research. Elsevier Biomedical Press, 147-149, p. 1975. , New York | ||
| 504 | |a Teutsch, G., Costerousse, G., Deraet, R., Benzoni, J., Fortin, M., Philibert, D., (1981) Steroids, 38, pp. 651-655 | ||
| 504 | |a Burton, G., Galigniana, M., Delavallaz, S., Brachet-Cota, A., Sproviero, A., Ghini, A., Lantos, C., (1995) Mol. Pharmacol., 47, pp. 535-543 | ||
| 504 | |a Fraser, R., Lantos, C., (1978) J. Steroid Biochem., 9, pp. 273-276 | ||
| 504 | |a Bradford, M., (1976) Analyt. Biochem., 72, pp. 248-256 | ||
| 504 | |a Krozowski, Z., Funder, J., (1983) Proc. Natl. Acad. Sci., 80, pp. 6056-6060 | ||
| 504 | |a Rafestin-Oblin, M., Michaud, A., (1977) J. Steroid Biochem., 8, pp. 19-23 | ||
| 504 | |a Pratt, W., Ishii, D., (1972) Biochemistry, 11, pp. 1401-1410 | ||
| 504 | |a Emadian, S., Luttge, W., (1988) J. Neurochem., 50, pp. 1456-1460 | ||
| 504 | |a Ermacora, M., Fox, R., (1992) Proc. Natl. Acad. Sci., 89, pp. 6383-6387 | ||
| 504 | |a Beeuwkes, R., Ichikawa, I., Brenner, B., (1981), The Kidney. B.Brenner and F.Rector (Eds.), 249-288, W.B.Saunders Co., Philadelphia; Bresnick, E., Sanchez, E., Pratt, W., (1988) J. Steroid Biochem., 30, pp. 267-269 | ||
| 504 | |a Grillo, C., Vallee, S., Piroli, G., McEwen, B., De Nicola, A., (1992) J.Steroid Biochem. Molec. Biol., pp. 42515-42520 | ||
| 504 | |a Ganesan, S., Bashirelahi, N., Young, J., Cohen, S., (1987) Life Sci., 41, pp. 2767-2776 | ||
| 504 | |a Lemesle-Varloot, L., Ojasoo, T., Mornon, J., Raynaud, J., (1992) J. Steroid Biochem. Mol. Biol., 4, pp. 369-388 | ||
| 504 | |a Hebbel, R., (1990) Semin. Hematol., 27, pp. 51-69 | ||
| 504 | |a Berg, J., (1986) Science, 232, pp. 485-487 | ||
| 504 | |a Stancato, L., Silverstein, A., Gitler, C., Groner, B., Pratt, W., (1996) J. Biol. Chem., 271, pp. 8831-8836 | ||
| 504 | |a Abate, C., Patel, L., Rauscher, F., Currant, T., (1990) Science, 249, pp. 1157-1161 | ||
| 504 | |a Grippo, J., Holmgren, A., Pratt, W., (1985) J. Biol. Chem., 260, pp. 93-97 | ||
| 504 | |a Silva, C., Cidlowski, J., (1992) J. Steroid Biochem. Molec. Biol., 41, pp. 1-10 | ||
| 520 | 3 | |a The purpose of this work is to review stability and activation properties of type 1 receptor, in order to explain the reasons for its extreme in vitro instability. We demonstrate that the treatment of rat kidney cytosol with H2O2 prevents aldosterone binding, DNA/steroid- receptor complex interactions, and prevents the receptor thermal inactivation. In contrast, exogenous sulfhydryl reducing reagents are necessary to insure maximum binding of mineralocorticoid receptor and DNA/steroid-receptor interaction. However, the presence of β- mercaptoethanol in thermal induced incubations reverts the H2O2 protection. We also demonstrate that contaminations with free or sequestered iron are harmful for both, receptor binding capacity (in a reversible form) and for hormone-receptor/DNA binding properties (in a partially reversible form). We propose a sulfhydryl oxidative mechanism for type I mineralocorticoid receptor inactivation in which iron contaminants might accelerate this process by oxidative catalysis. We also demonstrate that when thiol groups are blocked by specific reagents such as N-ethyl-maleimide or dithionitrobenzoic acid, type I sites loose binding capacity, but the protein is protected from oxidation as well as inactivation. |l eng | |
| 593 | |a Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires (1428), Argentina | ||
| 690 | 1 | 0 | |a MINERALOCORTICOID RECEPTOR |
| 690 | 1 | 0 | |a OXIDATION |
| 690 | 1 | 0 | |a STABILITY |
| 690 | 1 | 0 | |a THIOL GROUPS |
| 690 | 1 | 0 | |a 5,5' DITHIOBIS(2 NITROBENZOIC ACID) |
| 690 | 1 | 0 | |a ALDOSTERONE |
| 690 | 1 | 0 | |a DNA |
| 690 | 1 | 0 | |a EDETIC ACID |
| 690 | 1 | 0 | |a EGTAZIC ACID |
| 690 | 1 | 0 | |a HYDROGEN PEROXIDE |
| 690 | 1 | 0 | |a IRON |
| 690 | 1 | 0 | |a MERCAPTOETHANOL |
| 690 | 1 | 0 | |a MINERALOCORTICOID RECEPTOR |
| 690 | 1 | 0 | |a N ETHYLMALEIMIDE |
| 690 | 1 | 0 | |a STEROID |
| 690 | 1 | 0 | |a THIOL GROUP |
| 690 | 1 | 0 | |a ANIMAL CELL |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a CELL FREE SYSTEM |
| 690 | 1 | 0 | |a CYTOSOL |
| 690 | 1 | 0 | |a DNA BINDING |
| 690 | 1 | 0 | |a HIGH TEMPERATURE PROCEDURES |
| 690 | 1 | 0 | |a KIDNEY |
| 690 | 1 | 0 | |a NONHUMAN |
| 690 | 1 | 0 | |a OXIDATION |
| 690 | 1 | 0 | |a RAT |
| 690 | 1 | 0 | |a RECEPTOR BINDING |
| 690 | 1 | 0 | |a THERMOSTABILITY |
| 690 | 1 | 0 | |a ANIMALIA |
| 773 | 0 | |d Elsevier Inc., 1996 |g v. 59 |h pp. 511-521 |k n. 7 |p LIFE SCI. |x 00243205 |w (AR-BaUEN)CENRE-749 |t Life Sciences | |
| 856 | 4 | 1 | |u https://www.scopus.com/inward/record.uri?eid=2-s2.0-0030581094&doi=10.1016%2f0024-3205%2896%2900331-1&partnerID=40&md5=e89811f76604df37c751dfdef02ce2f7 |y Registro en Scopus |
| 856 | 4 | 0 | |u https://doi.org/10.1016/0024-3205(96)00331-1 |y DOI |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_00243205_v59_n7_p511_Galigniana |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_00243205_v59_n7_p511_Galigniana |y Registro en la Biblioteca Digital |
| 961 | |a paper_00243205_v59_n7_p511_Galigniana |b paper |c PE | ||
| 962 | |a info:eu-repo/semantics/article |a info:ar-repo/semantics/artículo |b info:eu-repo/semantics/publishedVersion | ||
| 999 | |c 64479 | ||