Porphyrinogen carboxylyase. Studies on the existence of isoenzymes.
Porphyrinogen carboxylyase from normal rat liver was subjected to purification methods. Two different purification protocols were used. In both cases, the initial steps consisted in obtaining a liver homogenate, followed by centrifugation, salt precipitation and phosphate gel absorption. Scheme I co...
Guardado en:
| Autor principal: | |
|---|---|
| Otros Autores: | , , , |
| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
1996
|
| Acceso en línea: | Registro en Scopus Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| LEADER | 03437caa a22005177a 4500 | ||
|---|---|---|---|
| 001 | PAPER-3427 | ||
| 003 | AR-BaUEN | ||
| 005 | 20230518203253.0 | ||
| 008 | 190411s1996 xx ||||fo|||| 00| 0 eng|d | ||
| 024 | 7 | |2 scopus |a 2-s2.0-0030325438 | |
| 024 | 7 | |2 cas |a porphyrin, 24869-67-8; porphyrinogen, 4396-11-6; Isoenzymes; Porphyrinogens; Porphyrins | |
| 040 | |a Scopus |b spa |c AR-BaUEN |d AR-BaUEN | ||
| 100 | 1 | |a Ríos de Molina, M.C. | |
| 245 | 1 | 0 | |a Porphyrinogen carboxylyase. Studies on the existence of isoenzymes. |
| 260 | |c 1996 | ||
| 270 | 1 | 0 | |m Ríos de Molina, M.C. |
| 506 | |2 openaire |e Política editorial | ||
| 520 | 3 | |a Porphyrinogen carboxylyase from normal rat liver was subjected to purification methods. Two different purification protocols were used. In both cases, the initial steps consisted in obtaining a liver homogenate, followed by centrifugation, salt precipitation and phosphate gel absorption. Scheme I consisted in then submitted the preparation to DEAE-cellulose, followed by Sephacryl S-200 and Phenyl-sepharose sequential column chromatographies. Scheme II involved an affinity column followed by a Sephadex G-75 gel filtration column. In both cases, the enzyme was stored at -20 degrees C until its assay. The addition of 2mM dithiotreytol to the incubation media or to the enzyme extract before storage, did not help improve the activity nor the stability of the enzyme. Those fractions containing the maximal enzyme activity, detected using Uroporphyrinogen III or Pentacarboxy-porphyrinogen III as substrate, were not always present in the same tubes for the different columns employed. In addition, the degree of purification obtained in some steps was different according to the substrate employed. The results suggest the existence of at least two isoenzymes for rat liver porphyrinogen carboxy-lyase. |l eng | |
| 593 | |a Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina | ||
| 690 | 1 | 0 | |a ISOENZYME |
| 690 | 1 | 0 | |a PORPHYRIN |
| 690 | 1 | 0 | |a PORPHYRINOGEN |
| 690 | 1 | 0 | |a ANIMAL |
| 690 | 1 | 0 | |a ARTICLE |
| 690 | 1 | 0 | |a COMPARATIVE STUDY |
| 690 | 1 | 0 | |a ENZYMOLOGY |
| 690 | 1 | 0 | |a ISOLATION AND PURIFICATION |
| 690 | 1 | 0 | |a LIVER |
| 690 | 1 | 0 | |a METABOLISM |
| 690 | 1 | 0 | |a RAT |
| 690 | 1 | 0 | |a ANIMALS |
| 690 | 1 | 0 | |a ISOENZYMES |
| 690 | 1 | 0 | |a LIVER |
| 690 | 1 | 0 | |a PORPHYRINOGENS |
| 690 | 1 | 0 | |a PORPHYRINS |
| 690 | 1 | 0 | |a RATS |
| 700 | 1 | |a Chaufan, G. | |
| 700 | 1 | |a Iglesias, S. | |
| 700 | 1 | |a Billi de Catabbi, S. | |
| 700 | 1 | |a San Martín de Viale, L.C. | |
| 773 | 0 | |d 1996 |g v. 46 |h pp. 265-275 |k n. 4 |p Acta Physiol Pharmacol Ther Latinoam |x 03276309 |w (AR-BaUEN)CENRE-1948 |t Acta physiologica, pharmacologica et therapeutica latinoamericana : órgano de la Asociación Latinoamericana de Ciencias Fisiológicas y [de] la Asociación Latinoamericana de Farmacología | |
| 856 | 4 | 1 | |u https://www.scopus.com/inward/record.uri?eid=2-s2.0-0030325438&partnerID=40&md5=16c26c137aa18d2211cf5592fd479a34 |y Registro en Scopus |
| 856 | 4 | 0 | |u https://hdl.handle.net/20.500.12110/paper_03276309_v46_n4_p265_RiosdeMolina |y Handle |
| 856 | 4 | 0 | |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_03276309_v46_n4_p265_RiosdeMolina |y Registro en la Biblioteca Digital |
| 961 | |a paper_03276309_v46_n4_p265_RiosdeMolina |b paper |c PE | ||
| 962 | |a info:eu-repo/semantics/article |a info:ar-repo/semantics/artículo |b info:eu-repo/semantics/publishedVersion | ||