Suppression of SPARC expression by antisense RNA abrogates the tumorigenicity of human melanoma cells
Acquisition of invasive/metastatic potential is a key event in tumor progression. Cell surface glycoproteins and their respective matrix ligands have been implicated in this process. Recent evidence reveals that the secreted glycoprotein SPARC (secreted protein, acidic and rich in cysteine) is highl...
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| Autor principal: | |
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| Otros Autores: | , , , , , , |
| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
| Publicado: |
1997
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| Acceso en línea: | Registro en Scopus DOI Handle Registro en la Biblioteca Digital |
| Aporte de: | Registro referencial: Solicitar el recurso aquí |
| Sumario: | Acquisition of invasive/metastatic potential is a key event in tumor progression. Cell surface glycoproteins and their respective matrix ligands have been implicated in this process. Recent evidence reveals that the secreted glycoprotein SPARC (secreted protein, acidic and rich in cysteine) is highly expressed in different malignant tissues. The present study reports that the suppression of SPARC expression by human melanoma cells using a SPARC antisense expression vector results in a significant decrease in the in vitro adhesive and invasive capacities of tumor cells, completely abolishing their in vivo tumorigenicity. This is the first evidence that SPARC plays a key role in human melanoma invasive-metastatic phenotype development. |
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| Bibliografía: | Stetler-Stevenson, W.G., Aznavoorian, S., Liotta, L.A., Tumor cell interactions with the extracellular matrix during invasion and metastasis (1993) Annu. Rev. Cell Biol., 9, pp. 541-573 Stetler-Stevenson, W.G., Liotta, L.A., Kleiner, D.E., Extracellular matrix 6: Role of matrix metalloproteinases in tumor invasion and metastasis (1993) FASEB J., 7, pp. 1434-1441 Albelda, S.M., Role of integrins and other cell adhesion molecules in tumor progression and metastasis (1993) Lab. Invest., 68, pp. 4-17 Wewer, U.M., Taraboletti, G., Sobel, M.E., Albrechtsen, R., Liotta, L.A., Role of laminin receptors in tumor cell migration (1987) Cancer Res., 47, pp. 5691-5698 Lane, T.F., Sage, E.H., The biology of SPARC, a protein that modulates cell-matrix interactions (1994) FASEB J., 8, pp. 163-173 Sage, E.H., Bornstein, P., Extracellular proteins that modulate cell-matrix interactions (1991) J. Biol. Chem., 266, pp. 14831-14834 Tremble, P.M., Lane, T.F., Sage, E.H., Werb, Z., SPARC, a secreted protein associated with morphogenesis and tissue remodelling, induces expression of metalloproteinases in fibroblasts through a novel extracellular matrix-dependent pathway (1993) J. Cell Biol., 121, pp. 1433-1444 Porter, P.L., Sage, E.H., Lane, T.F., Funk, S.H., Gown, A.M., Distribution of SPARC in normal and neoplastic tissue (1995) J. Histochem. Cytochem., 43, pp. 791-800 Podhajcer, O.L., Comparative expression of the SPARC and stromelysin-3 genes in mammary tumors (1996) Breast, 5, pp. 13-20 Bellahcene, A., Castronovo, V., Increased expression of osteonectin and osteopontin, two bone matrix proteins, in human breast cancer (1995) Am. J. Pathol., 146, pp. 95-100 Porte, H., Neoplastic progression of human colorectal cancer is associated with overexpression of the stromelysin-3 and BM-40/SPARC genes (1995) Int. J. Cancer, 64, pp. 70-75 Ledda, F., The expression of the secreted protein acidic and rich in cysteine, SPARC, is associated with the neoplastic progression of human melanoma J. Invest. Dermatol., , in the press Podhajcer, O.L., Expression of cathepsin D in primary and metastatic human melanoma and dysplastic nevi (1995) J. Invest. Dermatol., 104, pp. 340-1144 Terranova, V.P., Williams, J.E., Liotta, L.E., Martin, C.R., Modulation of the metastatic activity of melanoma cells by laminin and fibronectin (1984) Science, 226, pp. 982-985 |
| ISSN: | 10788956 |
| DOI: | 10.1038/nm0297-171 |