Suppression of SPARC expression by antisense RNA abrogates the tumorigenicity of human melanoma cells

Acquisition of invasive/metastatic potential is a key event in tumor progression. Cell surface glycoproteins and their respective matrix ligands have been implicated in this process. Recent evidence reveals that the secreted glycoprotein SPARC (secreted protein, acidic and rich in cysteine) is highl...

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Autor principal: Fernanda Ledda, M.
Otros Autores: Adris, S., Bravo, A.I, Kairiyama, C., Bover, L., Chernajovsky, Y., Mordoh, J., Podhajcer, O.L
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 1997
Acceso en línea:Registro en Scopus
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Registro en la Biblioteca Digital
Aporte de:Registro referencial: Solicitar el recurso aquí
LEADER 06425caa a22009497a 4500
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024 7 |2 scopus  |a 2-s2.0-0031056656 
024 7 |2 cas  |a Oligonucleotides, Antisense; Osteonectin 
040 |a Scopus  |b spa  |c AR-BaUEN  |d AR-BaUEN 
030 |a NAMEF 
100 1 |a Fernanda Ledda, M. 
245 1 0 |a Suppression of SPARC expression by antisense RNA abrogates the tumorigenicity of human melanoma cells 
260 |c 1997 
270 1 0 |m Podhajcer, O.L.; Inst. de Investigaciones Bioquimicas, Fundacion Campomar, IIBBA-CONICET, Patricias Argentinas 435, 1405 Buenos Aires, Argentina 
506 |2 openaire  |e Política editorial 
504 |a Stetler-Stevenson, W.G., Aznavoorian, S., Liotta, L.A., Tumor cell interactions with the extracellular matrix during invasion and metastasis (1993) Annu. Rev. Cell Biol., 9, pp. 541-573 
504 |a Stetler-Stevenson, W.G., Liotta, L.A., Kleiner, D.E., Extracellular matrix 6: Role of matrix metalloproteinases in tumor invasion and metastasis (1993) FASEB J., 7, pp. 1434-1441 
504 |a Albelda, S.M., Role of integrins and other cell adhesion molecules in tumor progression and metastasis (1993) Lab. Invest., 68, pp. 4-17 
504 |a Wewer, U.M., Taraboletti, G., Sobel, M.E., Albrechtsen, R., Liotta, L.A., Role of laminin receptors in tumor cell migration (1987) Cancer Res., 47, pp. 5691-5698 
504 |a Lane, T.F., Sage, E.H., The biology of SPARC, a protein that modulates cell-matrix interactions (1994) FASEB J., 8, pp. 163-173 
504 |a Sage, E.H., Bornstein, P., Extracellular proteins that modulate cell-matrix interactions (1991) J. Biol. Chem., 266, pp. 14831-14834 
504 |a Tremble, P.M., Lane, T.F., Sage, E.H., Werb, Z., SPARC, a secreted protein associated with morphogenesis and tissue remodelling, induces expression of metalloproteinases in fibroblasts through a novel extracellular matrix-dependent pathway (1993) J. Cell Biol., 121, pp. 1433-1444 
504 |a Porter, P.L., Sage, E.H., Lane, T.F., Funk, S.H., Gown, A.M., Distribution of SPARC in normal and neoplastic tissue (1995) J. Histochem. Cytochem., 43, pp. 791-800 
504 |a Podhajcer, O.L., Comparative expression of the SPARC and stromelysin-3 genes in mammary tumors (1996) Breast, 5, pp. 13-20 
504 |a Bellahcene, A., Castronovo, V., Increased expression of osteonectin and osteopontin, two bone matrix proteins, in human breast cancer (1995) Am. J. Pathol., 146, pp. 95-100 
504 |a Porte, H., Neoplastic progression of human colorectal cancer is associated with overexpression of the stromelysin-3 and BM-40/SPARC genes (1995) Int. J. Cancer, 64, pp. 70-75 
504 |a Ledda, F., The expression of the secreted protein acidic and rich in cysteine, SPARC, is associated with the neoplastic progression of human melanoma J. Invest. Dermatol., , in the press 
504 |a Podhajcer, O.L., Expression of cathepsin D in primary and metastatic human melanoma and dysplastic nevi (1995) J. Invest. Dermatol., 104, pp. 340-1144 
504 |a Terranova, V.P., Williams, J.E., Liotta, L.E., Martin, C.R., Modulation of the metastatic activity of melanoma cells by laminin and fibronectin (1984) Science, 226, pp. 982-985 
520 3 |a Acquisition of invasive/metastatic potential is a key event in tumor progression. Cell surface glycoproteins and their respective matrix ligands have been implicated in this process. Recent evidence reveals that the secreted glycoprotein SPARC (secreted protein, acidic and rich in cysteine) is highly expressed in different malignant tissues. The present study reports that the suppression of SPARC expression by human melanoma cells using a SPARC antisense expression vector results in a significant decrease in the in vitro adhesive and invasive capacities of tumor cells, completely abolishing their in vivo tumorigenicity. This is the first evidence that SPARC plays a key role in human melanoma invasive-metastatic phenotype development.  |l eng 
593 |a Lnstituto de Invest. Bioquimicas, Fundacion Campomar, IIBBA-CONICET, Patricias Argentinas 435, 1405 Buenos Aires, Argentina 
593 |a Fac. Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, 1428 Buenos Aires, Argentina 
593 |a Hospital E. Peron, Balcarce 900, 1650 Provincia de Buenos Aires, Argentina 
593 |a Kennedy Institute of Rheumatology, 6 Bute Gardens, London W6 7DW, United Kingdom 
690 1 0 |a COMPLEMENTARY RNA 
690 1 0 |a GLYCOPROTEIN 
690 1 0 |a OSTEONECTIN 
690 1 0 |a ANIMAL EXPERIMENT 
690 1 0 |a ARTICLE 
690 1 0 |a CANCER INHIBITION 
690 1 0 |a CANCER INVASION 
690 1 0 |a CONTROLLED STUDY 
690 1 0 |a HUMAN 
690 1 0 |a HUMAN CELL 
690 1 0 |a MELANOMA CELL 
690 1 0 |a METASTASIS 
690 1 0 |a MOUSE 
690 1 0 |a NONHUMAN 
690 1 0 |a PRIORITY JOURNAL 
690 1 0 |a ANIMALS 
690 1 0 |a CELL ADHESION 
690 1 0 |a CELL DIVISION 
690 1 0 |a CELL MOVEMENT 
690 1 0 |a DOWN-REGULATION 
690 1 0 |a GENE EXPRESSION REGULATION, NEOPLASTIC 
690 1 0 |a HUMANS 
690 1 0 |a MELANOMA 
690 1 0 |a MELANOMA, EXPERIMENTAL 
690 1 0 |a MICE 
690 1 0 |a OLIGONUCLEOTIDES, ANTISENSE 
690 1 0 |a OSTEONECTIN 
690 1 0 |a TRANSFECTION 
690 1 0 |a TUMOR CELLS, CULTURED 
690 1 0 |a ANIMALIA 
700 1 |a Adris, S. 
700 1 |a Bravo, A.I. 
700 1 |a Kairiyama, C. 
700 1 |a Bover, L. 
700 1 |a Chernajovsky, Y. 
700 1 |a Mordoh, J. 
700 1 |a Podhajcer, O.L. 
773 0 |d 1997  |g v. 3  |h pp. 171-176  |k n. 2  |p NAT. MED.  |x 10788956  |w (AR-BaUEN)CENRE-6218  |t Nature Medicine 
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856 4 0 |u https://doi.org/10.1038/nm0297-171  |y DOI 
856 4 0 |u https://hdl.handle.net/20.500.12110/paper_10788956_v3_n2_p171_FernandaLedda  |y Handle 
856 4 0 |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_10788956_v3_n2_p171_FernandaLedda  |y Registro en la Biblioteca Digital 
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