Identification of cougar and jaguar feces through bile acid chromatography

The use of feces for population size assessment and diet analysis of carnivore species depends on accurate feces identification. Fecal bile acid composition appears to be species among carnivores and therefore a useful method to identify fecal samples. To help identify 194 field-collected feces of c...

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Autor principal: Fernández, G.J
Otros Autores: Corley, J.C, Capurro, A.F
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: Wiley-Blackwell 1997
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100 1 |a Fernández, G.J. 
245 1 0 |a Identification of cougar and jaguar feces through bile acid chromatography 
260 |b Wiley-Blackwell  |c 1997 
270 1 0 |m Fernandez, G.J.; Instituto de Biol. y Medicina Exptl., CONICET, Vuelta de Obligado 2490, (1428) Capital Federal, Argentina 
506 |2 openaire  |e Política editorial 
504 |a Aldred, D.R., (1980) Biochemical Identification of Carnivore Scats, 16p. , M.S. Thesis. Mississipi State Univ., Missisipi State 
504 |a Asa, C.S., Wallace, M.P., Diet and activity pattern of the Sechurian desert fox (Dusicyon sechurae) (1990) J. Mammal., 71, pp. 69-72 
504 |a Clinite, E.W., (1981) Biochemical Analysis of Mountain Lion and Bobcat Scats: Difference between Species and Sex, 49p. , M.S. Thesis. San Jose State Univ., San José , Calif 
504 |a Capurro, A.F., Novaro, A.J., Travaini, A., Romero, M., Improved bile-acid thin-layer chromatography to identify feces of neotropical carnivores (1997) J. Wildl. Manage., , In Press 
504 |a Daner, D.A., Dodd, N., Comparison of coyote and grey fox scat diameters (1982) J. Wildl. Manage., 46, pp. 240-241 
504 |a Doncaster, C.P., Dickmen, C.R., Mac Donald, D.W., Feeding ecology of red fox (Vulpes vulpes) in the city of Oxford, England (1990) J. Mammal., 71, pp. 188-194 
504 |a Duran, J.C., Poiani, A., Raedeke, K., Food habits of Dusicyon griseus in Northern Chile (1984) J. Mammal., 65, pp. 515-517 
504 |a Jaksic, F.M., Yañez, J.L., Ran, J.R., Trophic relation of the southernmost populations of Dusicyon in Chile (1983) J. Mammal., 64, pp. 693-697 
504 |a Johnson, M.K., Aldred, D.R., Clinite, E.W., Kutilek, M.J., Biochemical identification of bobcat scats (1981) Proc. Bobcat Res. Conf., Nat. Wild. Fed. Sci. and Tech. Ser., 6, pp. 92-96 
504 |a Belden, R.C., Aldred, D.R., Differentiation of mountain lion and bobcat scats (1984) J. Wildl. Manage., 48, pp. 239-244 
504 |a Clark, T.W., Schroeder, M.H., Richardson, L., Fecal bile acids of black-footed ferrets (1986) Great Basin Nat., 8, pp. 141-144 
504 |a Kritchevsky, D., Martak, D.S., Rothblat, G.H., Detection of bile acids in thin-layer chromatography (1963) Anal. Biochem., 5, pp. 388-392 
504 |a Major, M., Johnson, M.K., Davis, W.S., Kellogg, T.F., Identifying scats by recovery of bile acids (1980) J. Wildl. Manage., 44, pp. 290-293 
504 |a Quinn, T., Jackman, W.R., Influence of diet on detection of fecal bile acids by thin-layer chromatography (1994) J. Wildl. Manage., 58, pp. 295-299 
504 |a Weaver, J.L., Fritz, S.H., Comparison of coyote and wolf scat diameters (1979) J. Wildl. Manage., 43, pp. 786-788 
504 |a Windberg, L.A., Mitchell, C.D., Winter diets of coyotes in relation to prey abundance in southern Texas (1990) J. Mammal., 71, pp. 439-447 
520 3 |a The use of feces for population size assessment and diet analysis of carnivore species depends on accurate feces identification. Fecal bile acid composition appears to be species among carnivores and therefore a useful method to identify fecal samples. To help identify 194 field-collected feces of cougars (Puma concolor) and jaguars (Felis onca), 2 sympatric carnivore species of the Chaco Regia (Paraguay), we developed a method to determine feces origin using thin layer chromatography (TLC) bile acid patterns. Bile acids were extracted from 11 fecal samples from cougars and 10 from jaguars from different zoological gardens. Although cougar and jaguar feces contain the same identifiable bile acids, feces can be distinguished by the presence of a single unknown bile acid present consistently only among jaguar feces. The use of this bile acid as an indicator of feces origin, evaluated by means of 2 blind tests of 10 samples each, reached 80%. We also compared our results with a previous identification of the field collected feces based on physical characteristics. We suggest that TLC analysis of fecal bile acids is the best identification technique of cougar and jaguar feces technique available.  |l eng 
593 |a Departamento de Biología, Fac. de Ciencias Exactas y Naturales, Ciudad Universitaria, (1426) Buenos Aires, Argentina 
593 |a Departamento de Investigación, Universidad de Belgrano, Zabala 1851, (1426) Buenos Aires, Argentina 
593 |a Inst. de Biol. y Med. Experimental, CONICET, Vuelta de Obligado 2490, (1428) Capital Federal, Argentina 
651 4 |a CHACO REGION 
651 4 |a PARAGUAY 
651 4 |a CHACO 
690 1 0 |a CHROMATOGRAPHY 
690 1 0 |a COUGAR 
690 1 0 |a FECAL BILE ACIDS 
690 1 0 |a FECES 
690 1 0 |a FELIS ONCA 
690 1 0 |a JAGUAR 
690 1 0 |a PUMA CONCOLOR 
690 1 0 |a THINLAYER 
690 1 0 |a CYAMOPSIS TETRAGONOLOBA 
690 1 0 |a FELIS 
690 1 0 |a PANTHERA ONCA 
690 1 0 |a PUMA CONCOLOR 
700 1 |a Corley, J.C. 
700 1 |a Capurro, A.F. 
773 0 |d Wiley-Blackwell, 1997  |g v. 61  |h pp. 506-510  |k n. 2  |p J. WILDL. MANAGE.  |x 0022541X  |w (AR-BaUEN)CENRE-3198  |t Journal of Wildlife Management 
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