Regulation of ovarian follicle differentiation in gonadotrophin-stimulated rats

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The aim of the present study was to investigate the regulation of the in vitro DNA synthesis of ovarian cells recovered from prepubertal rats 48 h after administration of pregnant mare's serum gonadotrophin alone (granulosa cells) or followed by human chorionic gonadotrophin (luteal cells). Iso...

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Detalles Bibliográficos
Autor principal: Andreu, C.V
Otros Autores: Buscaglia, C.A, Parborell, F., Stein, P., Tesone, M.
Formato: Capítulo de libro
Lenguaje:Inglés
Publicado: 1997
Materias:
endocorion, elea, Argentina; novormon
Acceso en línea:Registro en Scopus
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Registro en la Biblioteca Digital
Aporte de:Registro referencial: Solicitar el recurso aquí
Biblioteca Central Dr. Luis F. Leloir (FCEN) de Universidad de Buenos Aires
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024 7 |2 scopus  |a 2-s2.0-0030972262 
024 7 |2 cas  |a chorionic gonadotropin, 9002-61-3; estradiol, 50-28-2; follitropin, 9002-68-0; gonadotropin, 63231-54-9; luteinizing hormone, 39341-83-8, 9002-67-9; seric gonadotropin, 9002-70-4; thymidine, 50-89-5; Culture Media, Conditioned; DNA, 9007-49-2; Gonadotropins; Gonadotropins, Equine; Steroids; Thymidine, 50-89-5 
040 |a Scopus  |b spa  |c AR-BaUEN  |d AR-BaUEN 
030 |a EOCRE 
100 1 |a Andreu, C.V. 
245 1 0 |a Regulation of ovarian follicle differentiation in gonadotrophin-stimulated rats 
260 |c 1997 
270 1 0 |m Tesone, M.; Inst. Biologia/Medicina Experimental, Obligado 2490, 1428 Buenos Aires, Argentina; email: mtesone@proteus.dna.uba.ar 
506 |2 openaire  |e Política editorial 
504 |a Auletta, F.J., Flint, A.P.F., (1988) Endocr. Rev., 9, pp. 88-105 
504 |a Keyes, P.L., Wiltbank, M.C., (1988) Ann. Rev. Physiol., 50, pp. 465-482 
504 |a Hsueh, A.J.W., Adashi, E.Y., Jones, P.B.C., Welsh Jr., T.H., (1984) Endocr. Rev., 5, pp. 76-127 
504 |a Bendell, J.J., Dorrington, J., (1991) Endocrinology, 128, pp. 2663-2665 
504 |a Peluso, J.J., Delidow, B.C., Lynch, J., White, B.A., (1991) Endocrinology, 128, pp. 191-196 
504 |a Hammond, J.M., Barañao, J.L.S., Skaleris, D.A., Knight, A.B., Romanus, J.A., Rechler, M.M., (1985) Endocrinology, 117, pp. 2553-2555 
504 |a Voutilainen, R., Miller, W., (1987) Proc. Natl. Acad. Sci. USA, 84, pp. 1590-1594 
504 |a Hirshfield, A., (1989) Growth Factors and the Ovary, , Plenum : New York 
504 |a Bley, M.A., Simón, J.C., Estévez, A.G., Jiménez De Asúa, L., Barañao, J.L., (1992) Endocrinology, 131, pp. 1223-1229 
504 |a Bley, M.A., Simón, J.S., Saragüeta, P.E., Barañao, J.L.S., (1991) Biol. Reprod., 44, pp. 880-888 
504 |a Roy, S.K., Greenwald, G.S., (1991) Biol. Reprod., 44, pp. 889-896 
504 |a Zhou, J., Chin, E., Bondy, C., (1991) Endocrinology, 129, pp. 3281-3288 
504 |a Yong, E.L., Baird, D.T., Yates, R., Reichert Jr., L.E., Hillier, S.G., (1992) J. Clin. Endocrinol. Metab., 74, pp. 842-849 
504 |a Maruo, T., Ladines-Llave, C.A., Samoto, T., Matsuo, H., Manalo, A.S., Ito, H., Mochizuki, M., (1993) Endocrinology, 132, pp. 924-931 
504 |a Dain, L.B., Stein, P., Krimer, A.R.D., Asch, R.H., Polak De Fried, E., Charreaue, H., Tesone, M., (1991) Fertil. Steril., 55, pp. 1093-1098 
504 |a Chaffkin, L.M., Luciano, A.A., Peluso, J.J., (1992) J. Clin. Endocrinol. Metab., 75, pp. 1404-1408 
504 |a Bomsel-Helmreich, O., Gougeon, A., Thebault, A., Saltarelli, D., Milgram, E., Frydman, R., Papiernik, E., (1979) J. Clin. Endocrinol. Metab., 48, pp. 686-694 
504 |a Dain, L.B., Bley, M.A., Barañao, J.L., Tesone, M., (1993) Mol. Reprod. Dev., 36, pp. 159-163 
504 |a Greenwald, G.S., Roy, S.K., (1994) The Physiology of Reproduction, pp. 629-724. , Knobil, E. and Neill, J. D. (eds.). New York: Raven 
504 |a Peluso, J.J., Steger, R.W., Hafez, E.S.E., (1976) J. Reprod. Fertil., 47, pp. 55-58 
504 |a Zarrow, M.X., Wilson, E.D., (1961) Endocrinology, 69, pp. 851-855 
504 |a Hirshfield, A., (1986) Biol. Reprod., 34, pp. 229-235 
504 |a Amsterdam, A., Rotmensch, S., (1987) Endocr. Rev., 8, pp. 309-337 
504 |a Yong, E.L., Baird, D.T., Hillier, S.G., (1992) Clin. Endocrinol., 37, pp. 51-58 
504 |a Bradbury, J.T., (1961) Endocrinology, 68, pp. 115-120 
504 |a Merk, F.B., Botticelli, C.R., Albright, J.T., (1972) Endocrinology, 90, pp. 992-1007 
504 |a Pencharz, R.I., (1940) Science, 91, p. 554 
504 |a Stumpf, W.E., (1969) Endocrinology, 85, pp. 31-37 
504 |a Tetsuka, M., Whitelaw, P.F., Bremner, W.J., Millar, M.R., Smyth, C.D., Hillier, S.G., (1995) J. Endocrinol., 145, pp. 535-543 
504 |a Hirai, M., Hirata, S., Osade, T., Hagihara, K., Kato, J., (1994) J. Steroid Biochem. Mol. Biol., 40, pp. 1-7 
504 |a Wilson, C.A., Ter Haar, M.B., Bonney, R.C., Buckingham, J., Dixson, A.F., Yeo, T., (1983) J. Endocrinol., 99, pp. 63-76 
504 |a Guerrero, H.E., Stein, P., Asch, R.H., Polak De Fried, E., Tesone, M., (1993) Fertil Steril., 59, pp. 803-809 
504 |a Bornstein, M.B., (1958) Lab Invest., 7, pp. 134-137 
504 |a Saragüeta, P., Krimer, A.R.D., Charreau, E.H., Tesone, M., (1989) J. Steroid Biochem., 32, pp. 393-397 
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520 3 |a The aim of the present study was to investigate the regulation of the in vitro DNA synthesis of ovarian cells recovered from prepubertal rats 48 h after administration of pregnant mare's serum gonadotrophin alone (granulosa cells) or followed by human chorionic gonadotrophin (luteal cells). Isolated granulosa cells were cultured in serum-free medium, different stimuli added for periods of 48 h, and 3H-thymidine incorporation was measured. Both follicle-stimulating hormone (FSH) and luteinizing hormone (LH) inhibited 3H-thymidine incorporation by cultured granulosa cells in a dose-dependent manner (FSH: 10, 100, 200 ng/mL = 26, 41, 49% inhibition, respectively; LH: 0.1, 1, 10 ng/mL = 11, 37, 75% inhibition, respectively). On the other hand, estradiol was found to stimulate 3H-thymidine incorporation in granulosa cells (Estradiol: 5, 50, 500 ng/mL = 17, 37, 76% stimulation, respectively). In luteal cells, the rate of basal 3H-thymidine incorporation was very low (granulosa cells: 2560 ± 310; luteal cells: 661 ± 92 cpm/100,000 cells) and not modified by any stimulus. To determine the possible production of an inhibitory growth factor by the early corpus luteum, 3H-thymidine incorporation by granulosa cells was assessed in the presence of 10% conditioned media (CM) recovered from luteal cell cultures. A marked inhibition both in basal and estradiol-stimulated 3H-thymidine incorporation was observed (74 and 76% of inhibition, respectively). Results suggest that an inhibitory growth factor produced by luteal cells after luteinizing gonadotrophin stimulus could be involved in the differentiation of growing follicles to corpus luteum.  |l eng 
536 |a Detalles de la financiación: Universidad de Buenos Aires 
536 |a Detalles de la financiación: Consejo Nacional de Investigaciones Científicas y Técnicas, T6cnicas, 0488/ 
536 |a Detalles de la financiación: Universidad de Buenos Aires 
536 |a Detalles de la financiación: The present study was funded by grants from the Consej o Nacional de Investigaciones Cientificas y T6cnicas (0488/ 88-National Research Council, Buenos Aires, Argentina), Roemmers Foundation (Buenos Aires, Argentina), and the Universidad de Buenos Aires (UBA EX-149-Buenos Aires, Argentina). 
593 |a Inst. de Biol. y Med. Experimental, CONICET, Buenos Aires, Argentina 
593 |a Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina 
593 |a Inst. de Biol. y Med. Experimental, Obligado 2490(1428), Buenos Aires, Argentina 
690 1 0 |a CORPUS LUTEUM 
690 1 0 |a FOLLICLE DIFFERENTIATION 
690 1 0 |a GRANULOSA CELL 
690 1 0 |a OVARY 
690 1 0 |a CHORIONIC GONADOTROPIN 
690 1 0 |a ESTRADIOL 
690 1 0 |a FOLLITROPIN 
690 1 0 |a GONADOTROPIN 
690 1 0 |a LUTEINIZING HORMONE 
690 1 0 |a NOVORMON 
690 1 0 |a SERIC GONADOTROPIN 
690 1 0 |a THYMIDINE 
690 1 0 |a UNCLASSIFIED DRUG 
690 1 0 |a ANIMAL CELL 
690 1 0 |a ANIMAL EXPERIMENT 
690 1 0 |a ARTICLE 
690 1 0 |a CONTROLLED STUDY 
690 1 0 |a CORPUS LUTEUM 
690 1 0 |a FEMALE 
690 1 0 |a GRANULOSA CELL 
690 1 0 |a LUTEAL CELL 
690 1 0 |a NONHUMAN 
690 1 0 |a OVARY FOLLICLE 
690 1 0 |a PRIORITY JOURNAL 
690 1 0 |a RAT 
690 1 0 |a ANIMALS 
690 1 0 |a CELL DIFFERENTIATION 
690 1 0 |a CELLS, CULTURED 
690 1 0 |a CORPUS LUTEUM 
690 1 0 |a CULTURE MEDIA, CONDITIONED 
690 1 0 |a DNA 
690 1 0 |a FEMALE 
690 1 0 |a GONADOTROPINS 
690 1 0 |a GONADOTROPINS, EQUINE 
690 1 0 |a GRANULOSA CELLS 
690 1 0 |a OVARIAN FOLLICLE 
690 1 0 |a OVARY 
690 1 0 |a PREGNANCY 
690 1 0 |a RATS 
690 1 0 |a RATS, SPRAGUE-DAWLEY 
690 1 0 |a STEROIDS 
690 1 0 |a STIMULATION, CHEMICAL 
690 1 0 |a THYMIDINE 
653 0 0 |a endocorion, elea, Argentina; novormon 
700 1 |a Buscaglia, C.A. 
700 1 |a Parborell, F. 
700 1 |a Stein, P. 
700 1 |a Tesone, M. 
773 0 |d 1997  |g v. 6  |h pp. 145-151  |k n. 2  |p ENDOCRINE  |x 1355008X  |t Endocrine 
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856 4 0 |u https://hdl.handle.net/20.500.12110/paper_1355008X_v6_n2_p145_Andreu  |y Handle 
856 4 0 |u https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_1355008X_v6_n2_p145_Andreu  |y Registro en la Biblioteca Digital 
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999 |c 64194 

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